ndr epithelial and lymphoid cell lines method of detection biochemical subtilisin resistance
Báo cáo y học: "Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells" potx
Ngày tải lên :
13/08/2014, 01:21
... in infected Jurkat T cells The in vivo detection of ASP gives a novel tool to better understand how HIV-1 is involved in the development of immunodeficiency Methods Plasmids and antibodies The ... MH, Brunck TK: Human T -cell leukemia virus minus strand transcription in infected T-cells Bioch Biophys Res Comm 1989, 163:1006-1013 Cavanagh M-H, Landry S, Audet B, Arpin-Andre C, Hivin P, Paré ... morphology of the cell was assessed by NDIC White bars correspond to a scale of 10 μm ASP is expressed in infected Jurkat T cells Before analyzing the expression of ASP in infected cells, we first...
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Báo cáo y học: "Biochip sensors for the rapid and sensitive detection of viral disease" pps
Ngày tải lên :
14/08/2014, 14:21
... terms of speed of detection Rider et al [19] demonstrated the use of engineered B cells capable of detecting pathogens within minutes (Figure 2) Their CANARY sensor (cellular analysis and notification ... individual cells into sensors Infected sample Minimal sample preparation Probe array Cell- omic sensor Cells Multi-parameter detection Figure The concept of cell- omic sensors’ Cell- based detection ... been greatly expanded to include the production of all manner of arrayed probes: cells, glycans and carbohydrates, to name but a few The significance of the microarray to the field of infectious...
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Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
Ngày tải lên :
29/03/2014, 21:20
... mouse tissues and cell lines Int J Biochem Cell Biol 33, 491–498 29 Ahvazi B, Boeshans KM, Idler W, Baxa U & Steinert P (2003) Roles of calcium ions in the activation and activity of the transglutaminase ... localization of activated TGase 3, which was found to display a pattern distinct from that of TGase 1, was observed by the detection of in situ activities using this peptide Results Screening of candidate ... Thus, TGase and TGase appeared active in distinct regions of the hair follicle cells Discussion During differentiation of keratinocytes and hair formation, isopeptide cross-linking of several...
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Báo cáo sinh học: " Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases" doc
Ngày tải lên :
18/06/2014, 22:20
... infection prophylaxis and treatment are listed in Table Figure of strategies Targets different Biochemical Prevention and Treatment Targets of different Biochemical Prevention and Treatment strategies ... the inhibition of expression of specific genes by double-stranded RNAs (dsRNAs) It is becoming the method of choice to knockdown gene expression rapidly and robustly in mammalian cells Comparing ... endothelial cell membrane Palivizumab has been shown to reduce the rate of hospitalization of at-risk infants by about 55% in clinical studies and now serves as the primary medical means of RSV prevention...
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Báo cáo sinh học: " CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes" pptx
Ngày tải lên :
18/06/2014, 22:20
... [42] MfaRV2 Macaque fascicularis MneRV2 MndRHV1 MndlRHV2 MndsRHV2 panRHV1a panRHV1b RFHVMm Macaque nemestrina Mandrill sphinx Mandrill leucophaeus Mandrill sphinx Chimpanzee Chimpanzee Macaque ... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24 ... phylogenies and tracing of evolutionary pathways Furthermore, comparison of multiple representatives of homologous viral proteins can be of importance for understanding the protein structure and function...
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báo cáo hóa học:" Biochemical prevention and treatment of viral infections – A new paradigm in medicine for infectious diseases" pdf
Ngày tải lên :
20/06/2014, 04:20
... infection prophylaxis and treatment are listed in Table Figure of strategies Targets different Biochemical Prevention and Treatment Targets of different Biochemical Prevention and Treatment strategies ... the inhibition of expression of specific genes by double-stranded RNAs (dsRNAs) It is becoming the method of choice to knockdown gene expression rapidly and robustly in mammalian cells Comparing ... endothelial cell membrane Palivizumab has been shown to reduce the rate of hospitalization of at-risk infants by about 55% in clinical studies and now serves as the primary medical means of RSV prevention...
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báo cáo khoa học: " Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen" pot
Ngày tải lên :
12/08/2014, 03:20
... for isoform 02C01 and comprises 17% of all fragment IIIvariants, while peptides IIIb and Xb are unique for 01B01 and comprise 1819% of all fragment III and X-variants The isoforms 02A01 and 02B01 ... presence and relative abundances of individual isoforms The pollen of four birch species contained a mixture of Bet v isoforms, with abundant levels of isoforms that were similar to isoforms with ... C-terminus of Arginine and Lysine Fig shows an example of the fragments I to XVII that are theoretically formed after tryptic digestion of isoforms from the subfamilies 01 to 05 Isoforms of different...
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Báo cáo y học: "Eukaryotic large nucleo-cytoplasmic DNA viruses: Clusters of orthologous genes and reconstruction of viral genome evolution" pps
Ngày tải lên :
12/08/2014, 04:21
... of humans and other mammals These viruses infect animals and diverse unicellular eukaryotes, and replicate either exclusively in the cytoplasm of the host cells, or possess both cytoplasmic and ... of genes of cellular organisms, in all likelihood, were acquired in the course of evolution of the mimivirus lineage, probably, from a variety of distinct cellular sources; the same process of ... potential of comparative and evolutionary genomics of the NCLDV increase A pre-requisite of an informative comparative-genomic study of any group of organisms is an accurate delineation of the sets of...
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Báo cáo y học: " Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies" potx
Ngày tải lên :
13/08/2014, 01:20
... Diagnostics and the NCRR grant to the Yerkes National Primate Center DRR000165 and to RHS, grants from NIH (NCI) CA103943, US Department of Defense W81XWH-07-1-338; and Mal and Lea Bank and (to RHS and ... (B) Linear regression of signals at 10, and ng/ml of anti-His monoclonal antibody Detection limit was determined based on the linear fitting equation with a cutoff value of 1000 RLU Mab) By linear ... HIV and HTLV are also primarily to the envelope proteins (HIV-gp120, HTLV-gp46), transmembrane proteins (HIV-gp41, HTLV-gp21), and core proteins of capsid and matrix (HIV-p24 and p17, HTLVp24 and...
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Báo cáo y học: "Oral keratinocytes support non-replicative infection and transfer of harbored HIV-1 to permissive cells" ppsx
Ngày tải lên :
13/08/2014, 05:21
... fraction of VLPs that resisted trypsinization and were sensitive to cold and colchicine appeared to be harbored within TERT-2 cells and transferred to MOLT4/CCR5 cells by direct cell- to -cell interactions ... TERT-2 cells is suggested therefore to involve direct cell- to -cell interactions with PBMCs and other permissive cells In the oral mucosa, the transfer of infectious virus to proximal lymphoid cells ... TERT-2 cells were negative for CD4 as expected (Table 1) and 80% of the cells were positive for CD104, a β4-integrin chain generally expressed by epithelial cells [32] (Table 1) TERT-2 cells were...
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Báo cáo y học: "Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model" pptx
Ngày tải lên :
13/08/2014, 05:21
... CTLs and HTLV-I infected T cells We have previously established a rat model of ATL-like disease, which allows examination of the growth and spread of HTLV-I infected cells, as well assessment of ... Establishment of MOLT-4 cells stably expressing SCTs of RT1.Al To examine the effects of rat SCTs expressed on human cells and the influence of linker length on the activation of CTLs in more ... expression level of RT1.Al on MOLT-4 cells As shown in Figure 2A, equivalent levels of SCT expression were confirmed on the surface of MOLT4/RT1AlSCTax180S and MOLT-4/RT1AlSCTax180L cells, Page of 16 (page...
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Báo cáo y học: "Common principles and intermediates of viral protein-mediated fusion: the HIV-1 paradigm" pps
Ngày tải lên :
13/08/2014, 05:21
... intracellular activation of the fusion protein makes the cell- cell fusion model unsuitable for functional studies The use of cell- cell fusion assays is also limited when surface expression of viral ... model of lipid bilayer fusion The stalk-pore model of lipid bilayer fusion (A) and consensus models for class I and class II protein-mediated membrane fusion (B and C) SU and TM are the surface and ... host cell permit direct assessment of the extent and kinetics of virus -cell fusion [33-37], but these assays have limited sensitivity and temporal resolution A powerful approach to study virus-cell...
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Báo cáo y học: " PRMT6 diminishes HIV-1 Rev binding to and export of viral RNA" pps
Ngày tải lên :
13/08/2014, 09:20
... methylation of Rev by PRMT6 in vitro A, Sequences of recombinant histidine-tagged Tat86 and Rev Both sequences are chimeric and consist of BH10 (amino acids 2–66 and 2–15, respectively) and HXB2 ... 6% of control and R35K was not detectable The R39A and R39K substitutions resulted in band intensities that were either undetectable or 3% of wild-type, respectively Methylation of the R38A and ... calculated per amount of Rev in Rev only transfected cells and expressed as percentages The bars represent standard deviations of the mean of three independent experiments, each of which was carried...
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Báo cáo sinh học: "Analysis of a simulated microarray dataset: Comparison of methods for data normalisation and detection of differential expression (Open Access publication)" pps
Ngày tải lên :
14/08/2014, 13:22
... analysis methods Table contains the gene id (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and ... analysis methods The table contains the gene id (gene), mean and standard deviation of the unnormalised log ratio (M and SD), mean and standard deviation of the LOESS normalised log ratio (M LOESS and ... simulation software Array number of grid rows Array number of grid columns Number of spots in a grid row Number of spots in a grid column Number of spot pins Number of technical replicates Number of genes...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
Ngày tải lên :
11/09/2015, 10:06
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... 38 Fig DNA cell- cycle histograms of control (untreated) cells and TS-, SG-A-, and SG-B-treated cells for 24, 48, and 72 h, respectively Cells were fixed in 70% ethanol and stained with ... 6.2.1.5 HPLC Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods 80...
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Analysis and detection of human motion in time frequency domain
Ngày tải lên :
14/09/2015, 18:27
... previous activities of daily living (ADL) detection methods…… 21 2.2 Falls among the elderly and previous elderly fall detection methods…………28 2.3 Review of previous falls and ADL detection research ... decomposition and reconstruction process, we still need to understand the nature of the frequency bandwidth of decomposed and reconstructed signals Table 3.1 shows the frequency bandwidths of decomposed and ... coefficients and 93 separated segments of human motion patterns in 12 the acceleration signals 4.20 44 pairs of Pa and Pv for ascending and descending stairs 96 and 66 pairs of Pa and Pv for level...
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Analysis and detection of human emotion and stress from speech signals
Ngày tải lên :
15/09/2015, 22:03
... fi center frequency of i subband bi bandwidth of i subband α logarithmic growth factor C bandwidth of first filter lm lower edge of m th filter bank hm upper edge of m th filter bank St ( m ) ... Kyi Tar Nwe and other family members for their support, understanding and encouragement i Table of Contents Acknowledgements i Table of Contents ii Summary vi List of Symbols viii List of Figures ... Motivation of This Research 1.5 System Overview 1.6 Purpose and Contribution of This Thesis 10 1.7 Organization of Thesis 11 Chapter 2: Review of Acoustic Characteristics and Classification Systems of...
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Application of support vector machine to prediction of cross reactive allergens and recognition of viral sequences
Ngày tải lên :
30/09/2015, 13:49
... receptors on mast cells and basophils This triggers effector cell degranulation and rapid release of stored mediators of allergic reactions (e.g histamines), and secretion of cytokines (Galli ... implementation of SVM (Chang and Lin, 2001) 3.2.4 Measurement of Prediction Performance Performance of VIPR on the independent data set was measured with the same method as the measurement of performance of ... these methods show that the Codex guidelines is the most suitable candidate for evaluating of allergenicity potential, based on its current state of knowledge regarding food allergens and risk...
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Comparative studies on the infection and colonization of maize leaves by fusarium graminearum, f proliferatum and f verticillioides
Ngày tải lên :
19/11/2015, 16:47
... pathogens showed intercellularand intracellular infection of epidermal and mesophyll cells.Additionally,F.graminearumhyphaewerefoundinsclerenchymacells,xylemandthe phloemvesselsofdetachedleaves.Thesuperficialhyphaeandreemerginghyphaeofthe ... moniliforme and F. subglutinans in 1983. In AustraliaandintheUnitedStates,F.graminearumwascapableofcausingheadblightof wheat,crownrotofwheatand stalkrotofmaize.F.culmorumfromfootrotofwheat and barley ... old stems andoncobsofmaize.Thestrawanddebrisofwheat,barleyandothercerealarethe mainreservoirofF.graminearum.Chlamydosporesorperitheciaofthisfungusformed on debris can survive over winter and infect...
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