nanoparticles as adjuvant vectors for vaccination

... water each for 30 After drying in the air, the FTO glass was immersed in Au NP colloid solution at 70°C for 30 TiO2 film was prepared by screen printing of TiO2 paste on the FTO glass, followed ... citrate tribasic dehydrate, potassium carbonate anhydrous and tannic acid [26] The concentration of the Au NPs is about 0.3 mM Prior to the fabrication of TiO2 film, FTO glass was ultrasonically ... the performance enhancement of the cell Experimental FTO glass (resistivity: 14 X/h, Nippon Sheet Glass, Japan) was used as the substrate for nanocrystalline TiO2 (P25, Degussa) electrodes Cadmium...

Ngày tải lên: 21/06/2014, 07:20

... Conventional forms of gold nanoparticles are not good as contrast agents for SHG due to their symmetric structure Therefore asymmetric gold nanoparticles are required for SHG Such asymmetric gold nanoparticles ... ability of gold nanoparticles, it might appear that these nanoparticles would be promising contrast agents for SHG Unfortunately that is not the case Most common types of gold nanoparticles made ... Similarly SHG microscopy using gold particles has been reported [35] 1.5 Gold Nanoparticles Gold nanoparticles have been extensively used as contrast agents for optical microscopy because of their superior...

Ngày tải lên: 09/09/2015, 18:50

... amplified cDNA was 434 bp PCR was performed as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) ... mg/ml MTT solution was added to each well for incubation h Finally, the MTT was removed, and 200 μl DMSO was added to dissolve the MTT-formazan crystals The absorbance was measured at 490 nm by...

Ngày tải lên: 18/06/2014, 19:20

... amplified cDNA was 434 bp PCR was performed as follows: first cycle at 95°C for min, and then 30 cycles at 94°C for 45 s, 54°C for min, 72°C for and a final extension cycle of 72°C for The house-keeping ... tendency like as the free FA-PEAs (data not shown) So far as FA-PEAs:pVHL complexes were concerned, as shown in Table 2, the particle size was 277.5 nm at the mass ratio (FA-PEAs versus pVHL) ... mg/ml MTT solution was added to each well for incubation h Finally, the MTT was removed, and 200 μl DMSO was added to dissolve the MTT-formazan crystals The absorbance was measured at 490 nm by...

Ngày tải lên: 20/06/2014, 03:20

... micelles, the ethaselen-loaded micelle solution was lyophilized using PEG6000 as a lyoprotectant, and then dissolved in DMSO by ultrasonication for 15 min, and ethaselen content was measured with ... were then calculated based on the following formula: LCð%Þ mass of ethaselen extracted from freezeÀdried micelles ¼ total mass of freezeÀdried micelle Â100% EE ð%Þ mass of ethaselen extracted from ... compatibility between the micelle core-forming PLA and the ethaselen molecules In previous work, our group has prepared micellar ethaselen formulation employing PLA-based copolymers and characterized...

Ngày tải lên: 22/06/2014, 00:20

... [11] In this study therefore, efforts were made to determine the suitability of maize coated with V4 virus as a carrier in the vaccination of chickens against Newcastle disease Materials and methods ... husks), which was soaked in water for Page of three days, with a daily change of water (1:3; w/v) After the third day, it was sun-dried on a clean and well washed surface this was for the birds ... entire maize grain in water for four days after which it was ground and sieved The sieved out part (offal) was then sun dried This was used for the birds in cage This method was adopted by, [9,10]...

Ngày tải lên: 12/08/2014, 04:20

... intron A Insertion of the Kozak sequence increased the expression in all the cells whereas addition of the intron A decreased the expression In vectors used for DNA vaccines strong promoters are used ... and purification of vectors Origin and modification of vectors is shown in table and figure The HSA gene (1822 nucleotides, database no NM000477) was amplified by polymerase chain reaction (PCR) ... sequence, as W2 and V2 vectors that have both intron A and a Kozak sequence show similar or higher expression than G and H1 in the horse cells (Figure 3) The pBudCE4.1/lacZ/CAT plasmid was used as a...

Ngày tải lên: 12/08/2014, 18:22

... the malignant tumours was the increased vasculature Therefore, targeting tumour vasculature rather than the tumour cell itself as a treatment for cancer has gained increasing interest in recent ... fully realized; however, the feasibilities leading to this accomplishment are within close reach [25] (C) Adeno-associated virus (AAV)-based vectors AAV-based vectors have been shown to be non-toxic ... [27] (D) Herpesvirus-based vectors Vectors based on herpesviruses are well-developed and have progressed to clinical trials As with other viral vector, replication defective vectors did not show...

Ngày tải lên: 14/08/2014, 19:22

... chemoresistance ALDH3A1 was originally designated as the tumor ALDH, as it was found highly expressed in some human tumors such as hepatoma, lung adenocarcinoma, myeloma, breast cancer as well as in stem cell ... Aldehyde Dehydrogenase ALDH1A1 Aldehyde Dehydrogenase class A1 (cytosol) ALDH2 Aldehyde Dehydrogenase class (mitochondria) ALDH3A1 Aldehyde Dehydrogenase class A1 (cytosol) ... mechanisms of dehydrogenase and esterase assay catalyzed by ALDH3A1 The standard condition for a 15 minute linear reaction is also shown 27 ALDH2 and ALDH1A1 based activity was assayed spectrophotometrically...

Ngày tải lên: 24/08/2014, 09:51

Ngày tải lên: 08/08/2012, 17:20

... release The releasing curves of pravastatin show release phases, the first is rapid release phase and the second is slow sustained release phase with hemoglobin release similar pattern Pravastatin ... Osmotic fragility was studied for each drug concentration In vitro releasing study The release of pravastatin as well as hemoglobin from carrier erythrocytes were determined as following, ml of ... can be used for sustained release of pravastatin This assumption is supported by previous findings which recommended that the use of carrier erythrocytes as extended release system for prednisolone...

Ngày tải lên: 25/10/2012, 11:10

... sample was perceived to be slightly less pleasant in the second tasting session than in the first one, whereas the pleasantness of the heightened aroma sample increased in the second tasting session ... sample was rated as less pleasant than the regular aroma sample (Fð1; 106Þ ¼ 54:90; p # 0:001) However, the difference between the pleasantness ratings of the two samples was smaller for the ... the one they thought was correct (identification) (for more information on the test, see (Thomas-Danguin et al., 2003a)) Data analysis Repeated measures analysis of variance was used to test the...

Ngày tải lên: 03/04/2013, 21:06

... easier than tasks which require separating fact from opinion • Tasks that require information relevant to the main theme tend to be easier than tasks which ask for irrelevant details • Tasks that ... than tasks which require processing more information • Tasks that require processing information from just one location in the text tend to be easier than tasks which require integrating information ... parts of aspects, analyze, interpret, evaluate and reason from it In some ways, these may be called communicative tasks as they involve sts’ feelings, attitudes, tastes and values as well as their...

Ngày tải lên: 07/09/2013, 13:37

... rather than simply as task orientated Zuboff (1988) describes such employees as `knowledge workers' If these knowledge workers are to thrive and Knowledge Management as a Catalyst for Innovation ... processes will act as rapid carrier vehicles for the increased dissemination of new knowledge For example, Nonaka and Takeuchi (1995) single out the new product development process as entraining and ... catering, a new menu in California is in the best restaurants in Paris a fortnight later because you have chefs who travel the world.' Collaboration was seen as important for innovation through knowledge...

Ngày tải lên: 18/10/2013, 12:15

... access for users in heterogeneous networks by using products such as Services for UNIX, Client Services for NetWare, or Services for Macintosh Module 7: Microsoft Proxy Server 2.0 as a Solution for ... solution based on Windows 2000 As the director of information services, you are responsible for the transition from the mainframe solution to the Windows 2000–based solution The airline has three ... addresses that are assigned to the network segment that is directly connected to the interface The subnet mask assigned to the proxy server interface must match the subnet mask that is assigned to the...

Ngày tải lên: 18/10/2013, 18:15

... entries are supported for RIP -for- IP, RIP -for- IPX, and SAP -for- IPX, but not OSPF Module 8: Routing as a Solution for Private Network Connectivity 13 Including the RIP -for- IP Routing Protocol ... machine-based authentication is desired, and userbased authentication is insufficient A machine-based certificate infrastructure, such as Kerberos V5, exists Module 8: Routing as a Solution for Private ... routers with RIP -for- IP passwords, OSPF passwords, IPSec machine certificates, and demand-dial authentication Specifying RIP -for- IP or OSPF Passwords Specify RIP -for- IP or OSPF passwords to authenticate...

Ngày tải lên: 18/10/2013, 18:15

... performance Module 10: RADIUS as a Solution for Remote Access Introducing RADIUS Slide Objective To introduce RADIUS as a solution for remote access in a Windows 2000 network Lead-in Support for ... accounting 30 Module 10: RADIUS as a Solution for Remote Access Optimizing a RADIUS Design for Performance Slide Objective To describe the strategies used for optimizing the performance of a RADIUS solution ... locations, and the remote access accounting information This information provides the basic decisions for establishing a RADIUS remote access connection • Emphasize that separate remote access and user...

Ngày tải lên: 22/10/2013, 16:15

... liquid form and are usually prescribed by a physician for speciÞc diseases or disorders Medical foods are primarily intended for patients in hospitals or for individuals with rare diseases and ... cited in basic nutrition texts as being necessary for the metabolism of carbohydrates for energy Thus the association: because the drink contains these vitamins, energy is made available faster Some ... 3,3'-diindolylmethane (DIM) Sulforaphane is considered to be a potent regulator of these detoxiÞcation enzymes, Phase I and Phase II.188 Indole-3-carbinol may react with ascorbic acid to form ascorbigen, which...

Ngày tải lên: 25/10/2013, 21:20

... is associated, for example, with increased demand for inpatient psychiatric care (Abas et al., 2003), and, while poverty and substance abuse are not necessarily related, poverty often increases ... isolation, which is associated with decreased social support and increased burden for the family (Magliano et al., 2006) Social isolation is associated with poor outcome for people with schizophrenia, ... other areas of the United States 173 Social factors as a basis for treatment A jail diversion programme People suffering from both substance abuse disorder and serious mental illness form a significant...

Ngày tải lên: 01/11/2013, 09:20

... an associated default mask Classes with variable length subnet masks (VLSM) Classless Inter-Domain Routing (CIDR) with a specified prefix length Class-based networks support a single subnet mask, ... Host/Network D 224-239.xxx.xxx.xxx None Multicast groups E 240-255.xxx.xxx.xxx None Experimental Module 2: TCP/IP as a Solution for Networking Subnet Masks Class-based IP addresses are split into two ... Module 2: TCP/IP as a Solution for Networking Optimizing a TCP/IP Design for Performance Slide Objective To describe strategies for optimal IP traffic data transmission Lead-in The performance of...

Ngày tải lên: 21/12/2013, 05:18