nanofiber carbon nanoparticle nanodiamonds nanocomposite materials for decontamination of nerve agents
Synthesis of mannoside glycans of phosphatidylinositol mannosides (PIMs
... of Mannose 12 3.4 Optimizing in situ Coupling Method; Synthesis of a Model Trimannoside 13 3.5 Synthesis of Mannoside Glycan Units of PIMs 15 3.5.1 Synthesis of Dimannoside of ... Synthesis of Tetracyclic Core of Bielschowskysin 76 Scheme 1.12: Lear Synthesis of Tricyclic Core of Bielschowskysin 78 Scheme 1.13: Nicolaou Synthesis of Tetracyclic Skeleton of Bielschowskysin ... 3.3.2 Synthesis of Methoxy-1,2-Orthoester of Mannose Scheme 11: Synthesis of Mannose 1,2-Orthoester10 The 1,2-orthoester of mannose 19 that would be used for iterative amplification of mannose units...
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Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot
... blue G-250 Lane 1, lg of factor X; lane 2, 0.3 lg of factor X; lane 3, lg of factor IX; lane 4, lg of prothrombin; lane 5, lg of protein C; lane 6, lg of protein S; lane 7, lg of Gla-domainless factor ... method for the permethylation of carbohydrates Carbohydr Res 131, 209–217 40 Yu SY, Wu S & Khoo KH (2006) Distinctive characteristics of MALDI-Q/TOF and TOF/TOF tandem mass spectrometry for sequencing ... concentrations of standard FDP products (7.5, 14.3, 30, 60 and 120 lgÆmL)1) Acknowledgements We thank Ms Ying-Ming Wang for supplying venom cDNA and Mr Sz-Wei Wu for the MALDI-TOF/TOF 3956 MS/MS analyses of...
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Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
... monoisotopic [M-2CO2-H]– mass of 1840.42 Da) The mass shift of Da (MR ) M) confirms the formation of the two disulfide bridges However, comparison of the retention times of the hydrophilic tx5a, hydrophobic ... costs of the reagents and our desire to obtain peptides that were of the highest purity Also, the use of only a slight excess (10–20%) of some expensive amino acids contributed to the low yield of ... piperidine/DMF for Fmoc deprotection Coupling of Fmoc-Ala-Ala to Fmoc-Hypro Wang resin (50 mg) was performed manually and then loaded onto the automated synthesizer for the remainder of the sequence...
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Báo cáo khoa học: N-Glycan structures of squid rhodopsin Existence of the a1–3 and a1–6 difucosylated innermost GlcNAc residue in a molluscan glycoprotein pot
... Facilities for Proteomic research at the Academia Sinica, Taiwan, for the use of the MALDI-QTOF instrument This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, ... respectively The GU(ODS) and GU(amide) of these four glycans are summarized in Table On the basis of the GU data of glycan A, i.e GU(ODS) of 8.8 and GU(amide) of 5.6, the reference compound 010.1F, ... a mixture of trypsin and chymotrypsin, and the proteolysate was further digested with glycoamidase A to release N-glycans After removal of the peptide materials, the reducing ends of the N-glycans...
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Báo cáo khoa học: Glycan profiling of urine, amniotic fluid and ascitic fluid from galactosialidosis patients reveals novel oligosaccharides with reducing end hexose and aldohexonic acid residues ppt
... evidence for the presence of a group of oligosaccharides of composition H0–3N0–1S0–2 (g1–g11, Table 2) Tandem mass spectrometry indicated a sequence Hex–HexNAc–Hex–Hex or truncated versions thereof for ... mass loss of 18 Da from the Z1 ion For the fragment ion with mass m ⁄ z 220.9, carbon chain cleavages at C2–C3 and C4–C5 of the aldohexonic acid were assumed A linkage of hexose to the C4 of aldohexonic ... carbohydrate moieties of glycosphingolipids, and C1-oxidized (aldohexonic acid) carbohydrate moieties of glycosphingolipids On the basis of the presence of carbohydrate moieties of glycosphingolipids,...
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Báo cáo khoa học: Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan Glycoengineering for enhanced cell binding and serum clearance of the neoglycoprotein pot
... The affinity of binding of the monomeric galectins-3 and -5 was considerably reduced, arguing in favor of an influence of the strong cross-linking activity of galectin-1 as a clue for the functional ... Signals of NMR spectra were assigned according to the following convention including designation of spacer atoms illustrated for compound in Fig Preparation of neoglycoprotein A For conjugation of ... the presence of contaminating glycoforms, will account for a clear-cut correlation between structure and ligand properties Also, the assay deliberately avoided surface immobilization of the carbohydrate-binding...
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History Of Modern Biotechnology II
... precursors for cell wall components and enzymes for synthesis and transformation of wall materials, are formed at the endoplasmatic reticulum (ER), along the length of the active part of the hyphae ... a way of classifying objects on the basis of their form When the scientist becomes curious about the “why” of the development of a form he/she gets involved in the relationship between form and ... viewpoint This point of view is that of a former uni- Fig Development of models The Morphology of Filamentous Fungi versity professor, who started research after the morphology of moulds in 1971...
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History of Modern Biotechnology I - Springer
... ethanol Later, Chaim Weizmann of the UK applied the butyric acid bacteria, used for centuries for the retting of flax and hemp, for production of acetone and butanol His use of Clostridium during World ... not compete with chemical means for solvent production, it did provide a base of experience for the development of large scale cultivation of fungi for production of citric acid after the First ... number of British chemists, but the instability of the substance frustrated their efforts Eventually, a study began in 1939 at the Sir William Dunn School of Pathology of the University of Oxford...
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09 Physical and Chemical Characteristics of DDGS revisions.
... properties of bulk materials They also measure the amount of compaction as well as the bulk strength of materials (Rosentrater, 2006) Another approach for measuring the flowability of granular materials ... (lightness of color) and b* (yellowness of color), with an increase in a* (redness of color) Similar results were also reported by Ganesan et al (2005) Table The Effect of the Rate of Solubles ... increasing amount of surface area of an ingredient that is exposed and available for digestive enzymes to act upon Mixing efficiency – a more uniform particle size in a mixture of ingredients will...
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Use of DDGS in Swine Diets
... 1997; Pork Industry Handbook, 1998) As a result of limited information of feeding DDGS to sows, current recommendations for DDGS inclusion for use of DDGS in sow diets are somewhat different The ... basis as a source of supplemental amino acids at levels up to 44.2% of the diet for gestating sows Monegue and Cromwell (1995) compared reproductive performance of sows fed a fortified corn-soybean ... performance More recently, Gaines et al (2006) conducted two trials to evaluate the effect of dietary levels of DDGS and choice white grease on growth performance in the late nursery phase of...
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