naïve t cells generation of tregs and t cell suppression assay
báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
Ngày tải lên :
18/06/2014, 15:20
... Biosciences) The reactivity of CD8+ T cells to WT1 or CEA or both are shown as the percentage of the total population of CD8+ T cells that were double positive (CD8+pentamer+) Cytotoxicity assays The cytotoxicity ... [36,37] The fusion cells could prime naive T cells to differentiate into CTL with lytic activity against the HCC cells (Figure 3A and 3B) After hr coculture of the HCC cells with healthy donor's T cells ... supernatants in the generation of Treg in vitro is demonstrated in the present study, little is known about the impact of fusion cell vaccination on generating Treg The negative impact of fusion cell...
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Báo cáo khoa học: "Bilingual Hebrew-English Generation of Possessives and Partitives: Raising the Input Abstraction Level" pptx
Ngày tải lên :
08/03/2014, 06:20
... syntactic structure of a partitive structure, the part is the head of the phrase (and determines agreement), but the Thing - is what is being measured This creates an interesting difference ~)etween ... the existing SURGE input structure are the introduction of the re/-set feature and the update of the usage of the possessor feature For both of these features, the main requirement on the realizer ... distinguish between two sets of determiners, that they call D and Q quantifiers The distinction is based on syntactic features, such as position, ability to be modified, ability to participate...
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Báo cáo hóa học: "Photochemically reduced polyoxometalate assisted generation of silver and gold nanoparticles in composite films: a single step route" pptx
Ngày tải lên :
22/06/2014, 22:20
... shift of the BE energy of nanoparticles with respect to that of bulk metal is consistent with that reported in literature [31] The photoluminescence of silver and gold metals is generally attributed ... band position, the half width and the intensity change with the particle size The optical behavior of composite films was modulated using the dipping time intervals and concentration of the metal ... often need heat to enable them to proceed within minutes or days Controlled experiments were demonstrated that the silicotungstate ions are necessary for the rapid reduction of metal nanoparticles...
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Báo cáo hóa học: " Research Article Automatic Generation of Spatial and Temporal Memory Architectures for Embedded Video Processing Systems" docx
Ngày tải lên :
22/06/2014, 22:20
... It partitions the GMOs to ensure that their widths conform to those specified by the FPGA thus ensuring optimal usage of the block RAMs The algorithm takes advantage of the dual port capabilities ... interfaces and operators relative to each other The IMEM model can be imported into our automatic synthesis tool IMapper which generates the target-specific implementation of the interface and ... A tool implementing the methodology to generate optimized estimates of the memory requirements of an RTVPS was also presented Weinhardt and Luk [23] present a technique that optimally allocates...
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Generation of prosody and speech for mandarin chinese
Ngày tải lên :
17/09/2015, 17:20
... raw text Text analysis is to change the raw text into the format that prosody generation and synthesis parts can accept The raw text may consist of non-Chinese characters (symbols, digits, etc) ... we introduce some of the topics related to the research 1.1.1 Text-to-Speech Text-to-speech synthesis (TTS) is the automatic conversion of any plain text to speech (Shih and Sproat, 1996) The ... while the realization of prosody is either by transformation of the synthesis units or by selecting the proper units that match the target prosody 1.1.2 Prosody The ultimate goal of a TTS system...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
Ngày tải lên :
11/08/2014, 08:20
... corrected quantity of CD4 and CD8 mRNA as determined by QC-RT-PCR and the corresponding CD4 and CD8 counts of that patient as determined by flow cytometry are depicted in Table The patients' HIV status, ... development of competitor templates for quantitative-competitive RT-PCR (QC-RT-PCR) was similar to that of Lipman and coworkers [7] Oligonucleotide primers (Clontech, Palo Alto, CA) were chosen that ... 5'CAAGTTGTCATGGATGACC3' Discussion Our results indicate that there is no correlation between CD4 and CD8 mRNA levels and numbers of cells expressing these proteins on their surface The formation of...
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Báo cáo y học: " Human Immunodeficiency Virus Type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains" pot
Ngày tải lên :
13/08/2014, 05:22
... contributes to its enhancement of virion infectivity [18,21,23] We confirm here that in the context of T lymphocyte infection, Nef does not facilitate recruitment of HIV-1 Gag into DRMs and that ... host cell microdomain function Conclusion This study describes alterations of the lipidome as a new activity of the HIV pathogenicity factor Nef that might contribute to its multifaceted strategies ... demonstrated that this Nef activity depends on raft integrity of the producer cell and suggested this to reflect the Nef-mediated recruitment of HIV budding structures into DRMs [23] In line with a...
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synthesis and evaluation of 2,3-dihydroquinazolinones as dual inhibitors of angiogenesis and cancer cell proliferation
Ngày tải lên :
13/11/2014, 11:20
... series of ailments and wounds and the treatments that were used Among these, the papyrus describes cases of tumors of the breast and the methods used to treat them The author of this document, with ... cellular cytoskeleton They can be found distributed throughout the cellular cytoplasm and serve a variety of functions essential to the cellular life cycle They assist in the determination of cell ... cell cycle arrest and ultimately apoptosis Targeting tubulin and microtubules is a well-established method of combating malignant tumors One aspect that these tubulin drugs share with most other...
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Mechanisms of neurodegeneration and stem cell migration a study of molecular signals in the model of axotomy
Ngày tải lên :
16/09/2015, 17:12
... possibility to regain normal structure and function If target contact is not made, but the neuron survives, it will end up with an atrophic state The size of its perikaryon and the diameter of its ... in the model of axotomy It has been suggested that low ratio of Bcl-2 to Bax might be related to the high susceptibility of spinal motoneurons to axotomy-induced death after transection of the ... degeneration similar to the distal part within a short distance from the lesion site However, sprouting of fibres starts within 4-6 hours after axotomy The definitive sprouting of the majority...
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Báo cáo hóa học: "Comparison of anti-CD3 and anti-CD28-coated beads with soluble anti-CD3 for expanding human T cells: Differing impact on CD8 T cell phenotype and responsiveness to restimulation" pot
Ngày tải lên :
18/06/2014, 16:20
... (A and B) and CD8 (C and D) T cells To normalize for the impact of persistent growth attributable to primary stimulation, the ratio of growth after restimulation to growth by matched control cells ... could then be quantitated based on the pattern of CFSE fluorescence using Flo-Jo software, and the total number of viable cells per well Bulk stimulation of T cells in vitro To monitor cell phenotype ... establish that these phenotypically distinctive cells were derived from the naïve CD8 T cell subset The pattern of strong CD45RA expression in cultured CD8 T cells, is often associated with terminally...
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Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
Ngày tải lên :
19/06/2014, 08:20
... overlay of A and B, demonstrating the presence of the GFP containing chTCR cassette in all cells but lentiviruses have the capability to integrate into the genomes of non-replicating cells Whilst high ... preventing recognition by cytotoxic T cells Antibodies on the other hand retain their ability to recognize native antigens but in case of tumors their potential effects are compromised by their ... produced by PT67 packaging cells was 106 transforming units (TU)/ml when titrated in 293 cells This titer was adequate for infecting all MD45 cells but in contrast, 106 TU/ml was not sufficient to efficiently...
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báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
Ngày tải lên :
20/06/2014, 04:20
... overlay of A and B, demonstrating the presence of the GFP containing chTCR cassette in all cells but lentiviruses have the capability to integrate into the genomes of non-replicating cells Whilst high ... preventing recognition by cytotoxic T cells Antibodies on the other hand retain their ability to recognize native antigens but in case of tumors their potential effects are compromised by their ... produced by PT67 packaging cells was 106 transforming units (TU)/ml when titrated in 293 cells This titer was adequate for infecting all MD45 cells but in contrast, 106 TU/ml was not sufficient to efficiently...
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Báo cáo y học: "Heterogeneity of CD4+ and CD8+ memory T cells in localized and generalized Wegener’s granulomatosis." potx
Ngày tải lên :
09/08/2014, 01:21
... vitro to chemotactic gradients, cytokine release and cytotoxic activity of distinct T cell populations Detection of the inducible inflammatory chemokine receptors CCR5 and CCR3 on CD45RA+ T cells ... recruited to sites of inflammation [7,8] The phenotype of memory T cells is heterogeneous A fraction of CD45RO+ (positive for the short human isoform of CD45) memory T cells reverts to the phenotype ... described Thus, the local cytokine milieu might depend on the site and extent of disease activity [4,5] The shift in the cytokine profile in granulomatous lesions of the respiratory tract might be of...
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Báo cáo y học: "Role of regulatory T cells in experimental arthritis and implications for clinical use" potx
Ngày tải lên :
09/08/2014, 06:23
... treatment decreases the infiltrate in joints Furthermore, RA patients relapse shortly after withdrawal of anti-TNF-α [19] and thus, despite the dampening of joint inflammation and the reinstatement of ... active RA before treatment with anti-TNF-α were unable to suppress proinflammatory cytokine secretion from activated T cells and monocytes After anti-TNF-α treatment the ‘hibernated’ peripheral ... encephalomyelitis have highlighted the potential of this option by genetic manipulation of T cells [21,22] However, this might not be an easy road to follow in humans because not all autoantigen-specific T...
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Báo cáo y học: "Recruitment of dendritic cells and macrophages during T cell-mediated synovial inflammation" doc
Ngày tải lên :
09/08/2014, 10:22
... cell- mediated autoimmunity Firstly, activated CD4+ T cells from central lymph of arthritic donors transfer the disease without ex vivo stimulation [18] and the cells are recruited selectively to the SRT, ... that differentiation of monocytes is deviated toward DCs, while the smaller proportion of 'indeterminate' cells and the greater numbers of cells that express costimulatory molecules suggest that ... synoviocytes), with only scattered cells in the subintimal connective tissue These observations provide qualitative support to the quantitative studies on inflammatory cells obtained from SRT by...
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Báo cáo y học: "Indoleamine 2,3-dioxygenase-expressing dendritic cells are involved in the generation of CD4+CD25+ regulatory T cells in Peyer''''s patches in an orally tolerized, collagen-induced arthritis mouse model" ppt
Ngày tải lên :
09/08/2014, 10:22
... based on the maturation or activation state and the subset of DCs, and cytokine profiles in the microenvironment at the time of antigen uptake [1,14-16] A previous study demonstrated that CD11c+CD11b+ ... affect the T- cell response In the resting state, autoreactive T cells residing in the periphery are suppressed effectively by regulatory T cells, which are thought to prevent the development of ... 0.05) Without CII stimulation, 1-MT had no significant effect on T- cell proliferation in either group To investigate the effect of IDO on the suppression of antigenspecific T cells exerted by...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
Ngày tải lên :
12/08/2014, 11:23
... evaluation and to analysis of T- cell proliferation PM contributed to the design of the study and to manuscript preparation All authors contributed to interpretation of the data All authors read and ... reported that intravenous administration of the immortalized MSC cell line C3H1 0T1 /2 to immunized mice had no effect on the development of CIA [26] The treatment protocols and results of these studies ... detection of CFSE dilution by flow cytometry The percentage inhibition (100 × [(percentage of proliferating CD4+ cells not treated with MSCs – percentage of proliferating CD4+ cells treated with...
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Báo cáo y học: "Defective response of CD4+ T cells to retinoic acid and TGFb in systemic lupus erythematosus" ppt
Ngày tải lên :
12/08/2014, 17:22
... suggested that CD25- Tregs correspond to activated T cells without suppressive activity [13] The other group working with treated patients has shown that the CD25- Tregs retain a suppressive function, ... albeit incomplete, and have concluded that these cells represent an attempt to control active autoimmune activation [14] The size of the Treg compartment results from the combined contribution of ... demonstrated that the induction of Tregs by TGFb and RA was defective in SLE patients and that PBX1-d expression in CD4 + T cells impaired the regulation of FOXP3 and CD25 by TGFb and RA on these cells...
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Báo cáo y học: "Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules" ppt
Ngày tải lên :
13/08/2014, 05:21
... cocultured with MT-2 cells Thus, consistent with the ability of HTLV-I to induce transcription of several cellular genes, infection of lung epithelial cells with HTLV-I increased the production of ... chemokines and cell adhesion molecule in A549 cells cocultured with MT2 cells A549 cells were cocultured with MMC-treated MT-2 or CCRF-CEM cells At and days after cocultivation, A549 cells were harvested ... T- lymphocyte alveolitis in patients infected with HTLV-I, based on the cytotoxic immune response of CD8+ T cells [10], and the presence of circulating CD8+ cytotoxic T cells specific for the HTLV-I Tax...
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