mutation isolation and identification of auxotrophs of s typhimurium gfp

Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

Ngày tải lên : 07/08/2014, 18:21

... patterns between strains possessing only stx1 or stx2 and strains possessing both stx1 and stx2 RAPD has revealed that it could not discriminate among isolates according to their differences either ... length polymorphism (RFLP) analysis Therefore, RAPD fingerprinting analysis was used for this study because its advantages of time and cost-saving, sensitivity, and no special skills required to ... in livestock products Materials and Methods Bacterial strains E coli O157:H7 strains used in this study are listed in Table Four strains, one produces both Stx1 and Stx2, and one produces Stx1...

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Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt

Ngày tải lên : 07/08/2014, 23:22

... amplified from tissues and infected FCWF cells, while PCR for non-infected FCWF cells yielded no results By sequencing and analysis, the sequences from tissues and infected FCWF cells showed 100% ... indicates the inclusion bodies of GPV in the cytoplasm of cells Scale bars = 200 nm Isolation and identification of a canine coronavirus strain from giant pandas 263 isolated The electron microscopy ... virus References Fig Analysis of GPV based on gene sequencing (A) Comparison of amino acid sequences of S gene product from GPV by nested PCR assay with that of the S gene of canine coronavirus...

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Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx

Ngày tải lên : 13/08/2014, 15:21

... properties (Tables and 3) Anti-inflammation effects Systemic inflammation was suggested to be associated with increased risk of chronic diseases such as cardiovascular disease, cancer and insulin resistance ... IL-6, and MIP-2 productions using commercial ELISA kits NO was determined by Griess assay [17] three groups of diabetic rats The activity of hepatic glucose-6-phosphalase (G-6-Pase) and fasting serum ... mucin Muc5ac and iNOS in lung tissues and airway hyperresponsiveness [71] Andrographolide inhibits OVA-induced increases TNF-α and GM-CSF in BALF of OVA-sensitized and nasally-challenged mice...

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Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents

Ngày tải lên : 11/09/2015, 10:06

... structures of flavonoids, isoflavonoids and neoflavonoids 76 3.4.3 Skeletal structures of subgroups of flavonoids 76 HMBC and 66 NOESY XI 4.3.1.1 Effects of flavonoid compounds to on the viability of ... brain slices of rats subjected to MCA occlusion and reperfusion 130 6.3.3.2 Cleaved caspase-3 immunohistochemical staining of brain tissue sections of rats subjected to MCA occlusion and reperfusion ... enzymes and molecules 37 ARE-dependent luciferase reporter assay 37 Western blot analysis of cellular Nrf2 levels 38 GSH measurement 40 2.2.3 Statistical analysis 2.3 Results 41 42 2.3.1 Possible...

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báo cáo khoa học: " Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from Raphanus sativus (daikon radish)" ppt

Ngày tải lên : 12/08/2014, 03:21

... CH3SH CH3I (CH3) 2S Figure analysis of methyl halides, methanethiol, and DMS from R sativus GC-MS GC-MS analysis of methyl halides, methanethiol, and DMS from R sativus (a) GC-MS spectrum of methyl ... through roots and used as an HMT/HTMT substrate to form CH3I Partial purification and anion-specificity of HTMT from R sativus B oleracea possesses several isoforms of thiol methyltransferases, which ... 9:116 P P P Emission profiles and rates of CH3I from T aestivum, O sativa and R sativus The emission profiles of CH3I from three plant species were measured (Figure 1) These plants were cultured...

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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge

Ngày tải lên : 05/09/2013, 09:08

... µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent neutral sugar analysis showed that the neutral sugar was composed of rhamnose, fucose, ... (1996) for Aspergillus sp JS-42 Sato and Ose (1980) also reported that viscous substances extracted from activated sludge by NaOH flocculated kaolin and Escherichia coli suspensions below pH In ... were used for the flocculating test Muddy water was prepared as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand...

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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

Ngày tải lên : 18/02/2014, 16:20

... Austria) RT-PCR (Super Script Choice System for DNA Synthesis, Gibco BRL., Paisley, UK) was performed in two steps In the ﬁrst step, for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of ... and P IV isoforms, are shown in Fig Stretches of sequence that matched the calculated masses obtained by LC-MS ⁄ MS are underlined For example, our results showed that the ﬁrst ten residues of ... isoforms GalNAc This hypothesis was also raised for the mistletoe lectin I [29] The pairwise alignment of the isoforms reveals that, although the highest primary sequence similarity of each subgroup...

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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

Ngày tải lên : 21/02/2014, 00:20

... opening of the PHO active site Materials and methods Bacterial strain The A radioresistens S1 3 strain used in this work was isolated as previously described [16,17] This bacterium bears several ... 7.0, as eluent PHI molecular mass was confirmed by matrix-assisted laser desorption/ionization time -of- flight (MALDI-TOF) mass spectral analysis, using a Biflex mass spectrometer (Bruker) The sample ... rate was mLÆmin)1 Molecular mass determination The molecular mass was determined by means of SDS/ PAGE, size exclusion chromatography and mass spectrometry SDS/PAGE was carried out in separating...

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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

Ngày tải lên : 21/02/2014, 00:20

... reassessment of previous spectroscopic studies was undertaken, particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes In addition, the ﬁrst partial spectroscopic ... the NrfA and NrfH molecular masses, as further proved by the respective SDS/PAGE proﬁle (Fig 3C, inset) Densitometry analysis of the 61 kDa and 19 kDa bands (SDS/PAGE stained with Coomassie Brilliant ... high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for heme...

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Báo cáo " Analysis and identification of multi-variate random pressure fields using covariance and spectral proper transformations " pdf

Ngày tải lên : 05/03/2014, 14:20

... Effects of basic and cumulative spectral modes on spectral synthesis of pressure position Secondly, effects of basic and cumulative spectral pressure modes on the synthesis of the unsteady pressure ... meaning of the spectral pressure modes as well as the linkage between the pressure modes and hidden events of the unsteady pressure fields Synthesis and identification of random pressure field Firstly, ... consistent linkages between physical phenomena and POD mode due to series of physical measurements and POD analyses of pressure fields in low-rise buildings Effect of pressure tap positions on...

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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

Ngày tải lên : 07/03/2014, 21:20

... discussions The selection of the two-phase solvent system is the ﬁrst and critical step for an HPCPC or an HSCCC separation Successful separation by HSCCC or HPCPC depends on the suitable selection ... components degraded in aqueous solution [26] Hence, the non-aqueous solvent system was given priority in the selection of solvent system Eight kinds of two-phase nonaqueous solvent systems were studied, ... nonaqueous solvent system at low temperature to separate those unstable volatile components An HPCPC method using a nonaqueous two-phase solvent system was successfully developed for the separation of...

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Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Ngày tải lên : 08/03/2014, 16:20

... (2000) Antisense supression of 2-cysteine peroxiredoxin in Arabidopsis speci®cally Ó FEBS 2002 enhances the activities and expression of enzymes associated with ascorbate metabolism but not glutathione ... because of the presence of two conserved cysteines [5,6] Arabidopsis BAS1 was shown to be a chloroplastic protein The identity of our peptides with BAS1 and the presence of two cysteines in alignment ... primers used in cDNA synthesis were designed based on the amino-acid sequence of N- and C-terminal peptides, as determined by the procedure described above First-strand cDNA was ®rst synthesized...

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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

Ngày tải lên : 16/03/2014, 18:20

... to be assumed and was also established in previous experiments for other d-amino acids as discussed by Bruckner and ¨ Westhauser [5] Several enzymes might be synthesizing d-amino acids from l-amino ... in silico results were supported by Western blot analysis of isolated organelles and by the localization studies using fusions with GFP (Figs and 4) In general, the localization predictions of ... acids such as amino acid oxidases, transaminases, and racemases (epimerases) For example, in pea seedlings the occurrence of d-amino acid aminotransferase was demonstrated [36] For a number of...

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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain speciﬁc for the human mitochondrial translocase receptor Tom70 potx

Ngày tải lên : 17/03/2014, 10:20

... was used, biased toward the incorporation of cysteine at CDR3 loop residue positions and 7–11, to enhance the possibility of inter- and intra CDR disulphide cross-links These strategies are summarized ... 12F-11 paratope and assessment of the varying contributions of CDR and framework regions clearly requires deﬁnitive structural data; this analysis is currently in progress The isolation of 12F-11 from ... frequency histogram of IgNAR CDR3 loop lengths, from Wobbegong sharks (26 sequences) and Nurse sharks (35 sequences) (B) Schematic diagram of synthetic CDR 3s used in VNAR library construction, showing...

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Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx

Ngày tải lên : 23/03/2014, 07:20

... various times until colonies of similar sizes were formed Pulse-chase analyses Pulse-chase experiments were performed as described previously [7] Brieﬂy, 3.7 · 107 cells, grown at the permissive ... GL and KGM) GL is supported by the University Hospitals of Coventry and Warwickshire NHS Trust We are grateful for access and user support at the synchrotron facilities at ESRF, Grenoble and ... 27 Sikorski RS & Hieter P (1989) A system of shuttle vectors and yeast host strains designed for efﬁcient manipulation of DNA in Saccharomyces cerevisiae Genetics 122, 19–27 28 Gietz RD & Woods...

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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Ngày tải lên : 24/03/2014, 04:21

... primers, a full-length cDNA sequence was Fig SDS/PAGE analysis of puriﬁed bovine MUC15 Analysis was performed on 18% Tris/glycine polyacrylamide gels Positions of molecular mass standards are ... which did not show similarity to any known sequences as revealed by BLASTP and FASTA3 homology searching of databases accessed through the NCBI and EBI, respectively Sequence description of bovine ... in a variety of tissues and cell types by PCR screening The screening analysis was performed using commercial multiple tissue cDNA (MTC) panels of fetal and adult human tissues (human MTC Panel...

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Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

Ngày tải lên : 30/03/2014, 13:20

... expression of XHIVEP mRNAs Semiquantitative RT-PCR analysis was performed with RNA isolated from staged embryos (A), adult organs and tissues (B) and dissected regions of stage 10 embryos (C) making use ... at similar levels until the onset of gastrulation (egg until stage 10) Throughout late gastrula and neurula stages (stages 11– 20), expression decreases temporarily and increases again at stage ... expression of the XHIVEPs during gastrulation, when these signals play an essential role in patterning of the mesoderm Early gastrula stage embryos were dissected and total RNA isolated from pools...

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báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf

Ngày tải lên : 18/06/2014, 15:20

... culture of CD and non-CD subjects was done using one way ANOVA All statistical analysis was performed using statistical computer software (SPSS 13, SPSS, USA) A p value of 0.05 was considered significant ... cultures from endoscopic biopsies In the absence of standardised systems, we based FBs extraction method on those used for cultures from surgical pieces, muscle and skin tissue samples [28] Differently ... biopsies This success rate was not affected by other possible covariates such as the clinical and demographic characteristics of patients suggesting that the stabilization of cell culture is almost...

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Báo cáo sinh học: "Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" pot

Ngày tải lên : 19/06/2014, 08:20

... core-enriched samples without separation prior to trypsin digestion We used two different mass spectrometers to analyze the in-solution digests: MALDI-TOF/TOF MS and LC-ESI-Q-TOF MS This "shotgun" strategy ... Mass analysis of a distinct peptide from the E6R protein using Method (SDS-PAGE + LC-ESI-QIT MS) Mass analysis of a distinct peptide from the E6R protein using Method (SDS-PAGE + LC-ESI-QIT MS) ... and tandem mass analysis (LC-ESI-QIT MS) for protein identification had been used successfully before on other VV proteins [11] A second MS analysis was done in parallel with these samples using...

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