mtx cf101 on a3ar expression level in pha stimulated pbmcs from healthy individuals
Báo cáo y học: " Induction of multiple matrix metalloproteinases in human dermal and synovial fibroblasts by Staphylococcus aureus: implications in the pathogenesis of septic arthritis and other soft tissue infections" pdf
Ngày tải lên :
09/08/2014, 08:23
... changes in the expression levels of other MMP proteins (MMP-8 and MMP-9) in the cells in response to treatment The expression pattern and level of expression were similar in S aureus components ... 15 μg of protein from each fraction of culture supernatant per well (only from ATCC strain) Fibroblasts were cultured in an incubator containing a humidified atmosphere containing 5% CO2 at 37°C ... clearing infections, initiating immune responses, and in tissue remodeling [9] Excessive MMPs cause matrix degradation and joint destruction as in various forms of arthritis [10] Cytokines interleukin...
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Báo cáo y học: "Transcriptional regulation of matrix metalloproteinase-1 and collagen 1A2 explains the anti-fibrotic effect exerted by proteasome inhibition in human dermal fibroblasts" docx
Ngày tải lên :
12/08/2014, 12:20
... signaling functions of Smads This involves interactions with several proteins, such as Smad ubiquitination regulatory factors (Smurfs), the oncoprotein SnoN, and the multi-domain docking protein ... Permission to perform this investigation was granted by the ethics committee of our institution Informed consent was obtained in accordance with the Declaration of Helsinki Fibroblasts were maintained ... TGF-β-mediated increase in the production of type I collagen results from increased binding of transcription factors to three GC-rich SP1 sites (in the -303/-271 region) and one activation protein-1 (AP-1)...
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Báo cáo y học: " Effects of pro-inflammatory cytokines on expression of kynurenine pathway enzymes in human dermal fibroblasts" pdf
Ngày tải lên :
11/08/2014, 03:20
... studying the role of IDO1 in controlling experimental infections [24-26], expression or functionality of genes encoding downstream enzymes in the KP have not been investigated in such cells Since ... by incubation at 65°C for 10 according to the manufacturer’s instructions The DNase-treated RNA was subsequently reverse transcribed in 20 μl reactions containing the following reagents from Invitrogen; ... variation across individual Figure Relative levels of transcripts encoding enzymes in the kynurenine pathway in human skin-derived fibroblasts from individuals Transcripts encoding the following...
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Báo cáo y học: "Effects of pro-inflammatory cytokines on expression of kynurenine pathway enzymes in human dermal fibroblasts" pot
Ngày tải lên :
11/08/2014, 06:23
... studying the role of IDO1 in controlling experimental infections [24-26], expression or functionality of genes encoding downstream enzymes in the KP have not been investigated in such cells Since ... by incubation at 65°C for 10 according to the manufacturer’s instructions The DNase-treated RNA was subsequently reverse transcribed in 20 μl reactions containing the following reagents from Invitrogen; ... variation across individual Figure Relative levels of transcripts encoding enzymes in the kynurenine pathway in human skin-derived fibroblasts from individuals Transcripts encoding the following...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf
Ngày tải lên :
16/02/2014, 14:20
... the resulting MLL fusion proteins Thus, the MEN1 ⁄ LEDGF-interacting domain linked to DNA-binding domains (AT-hook and MT domain) becomes disconnected from the PHD domains, the FYRN domain, the ... different fusion proteins The common denominator in all different MLL rearrangements is disruption of the MLL protein in a region that prevents any subsequent protein–protein interaction between ... administered in a certain way (e.g chronic lymphoblastic leukemia) Replication of HIV-1 is also strongly inhibited by flavopiridol in low nanomolar concentrations, because transcription elongation...
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Báo cáo khoa học: Alternative splicing: role of pseudoexons in human disease and potential therapeutic strategies pot
Ngày tải lên :
06/03/2014, 09:22
... Cis-acting sequences in pseudoexon inclusion As previously mentioned, most pathological pseudoexon inclusion events originate from the creation of new splicing donor or acceptor splice sites within ... sequences in an unstructured conformation Trans-acting factors in pseudoexon inclusion Not many studies have focused on identifying the role played by trans-acting factors in pseudoexon inclusion However, ... First, U1snRNP binding to an intronic splicing processing element has been found to inhibit pathological pseudoexon inclusion in intron 20 of the ATM gene (Fig 3C) Inactivation of this element...
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Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf
Ngày tải lên :
06/03/2014, 22:21
... explain their wide distribution in tissues and cells [16], including stem cells [17] Increasing evidence links these noncatalytic actions with the binding of cholinesterases to several protein ... erythrocytes in the extent of binding with the lectins concanavalin A, Lens culinaris agglutinin 4522 (LCA), and Ricinus communis agglutinin (RCA) (Fig 3), ruled out the blood origin and supported the ... carcinomas In this respect, it is worth mentioning the recommended use of butyrylcholinesterase overexpression as a predictive survival index in liver cancer [51] Considering the butyrylcholinesterase...
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Báo cáo y học: "All-trans retinoic acid suppresses interleukin-6 expression in interleukin-1-stimulated synovial fibroblasts by inhibition of ERK1/2 pathway independently of RAR activation" ppsx
Ngày tải lên :
09/08/2014, 01:22
... basal conditions but were strongly expressed in response to IL-1β In IL-1 -stimulated synovial fibroblasts, ATRA (RAR agonist) decreased IL-6 gene expression from 70% (Figure 2a) and IL6 level from ... costimulation with ATRA and BMS-649 inhibited proinflammatory cytokine expression to the same extent as ATRA alone Effect of RAR-selective agonists on IL-1-induced expression of IL-6 As the RXR agonist ... interleukin-1-beta (IL-1β) on on the expression of retinoic receptor (RAR)(RAR) or retinoid X receptorsubtypes in synovial synovial Effect of interleukin-1-beta (IL-1β) the expression of retinoic...
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Báo cáo y học: "Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartate" pot
Ngày tải lên :
09/08/2014, 14:20
... d, demonstrating more intense GAG staining in the deep non-lesioned OA cartilage regions and less intense staining in lesioned cartilage (Figure 1d) compared with the superficial non-lesioned ... further analysis Determination of protein content Total protein was determined using the commercially available BCA™ Protein Assay Kit in accordance with the instructions of the manufacturer ... Histological section preparation and evaluation Cartilage sections, previously stained with Indian ink, were cut into 3-mm-thick pieces and embedded in Tissue Tek OCT Compound (Sakura Finetek USA, Inc.,...
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ADAM10 is expressed in human podocytes and found in urinary vesicles of patients with glomerular kidney diseases pptx
Ngày tải lên :
10/08/2014, 05:21
... podocyte specific marker proteins (integrin α3β1 or podocin) we confirmed that cells isolated from the urine are podocytes In addition, by intracellular FACS staining using ADAM10 and WT1 as a specific ... performed by incubating the tissue sections for 20 in 0.01 M sodium citrate buffer, pH 6.0, in a microwave oven (500 Watt) After incubation with blocking buffer (0.1% Triton X-100/PBS containing 1% ... 3F urinary podocytes only expressed low levels of L1, but L1 expression was induced after the treatment of the cells with proinflammatory cytokine IFN-γ (Fig 3F) In addition, L1 expression was...
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Damaged DNA-binding protein 2 (DDB2) protects against UV irradiation in human cells and Drosophila ppt
Ngày tải lên :
10/08/2014, 05:21
... overexpression of DDB2 using an adenovirus system increased the level of cFLIP in HR18 cells (Fig 3B) In this case, increased expression of DDB2 was noticed 24 hrs following virus infection, ... overexpression against UVinduced apoptosis (Fig 5A) Overexpression of DDB2 also decreased UV-induced cleavage of both DFF and PARP in HR18 cells (Fig 5B) In contrast, forced expression of control ... critical role in UV resistance In addition, forced expression of DDB2 in cFLIP-lacking cells (VA13 and XP-A) did not induce cFLIP accumulation, or protection against UV (Fig 6), indicating that the...
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Báo cáo khoa hoc:" Lack of association between sCTLA-4 levels in human plasma and common CTLA-4 polymorphisms" ppsx
Ngày tải lên :
11/08/2014, 07:20
... cellular level and circulating protein in plasma For example, elevated circulating sCTLA-4 levels may simply be due to increased half-life and/or decreased turnover of protein despite increased levels ... splicing) in immunoprecipi- tates from CTLA-4 monoclonal antibodies, only a minority of the material from these immunoprecipitation experiments is of the predicted molecular mass of the sCTLA-4 monomer ... number of subjects in each group See text for definitions of polymorphisms relations between absolute levels of sCTLA-4 protein and SNP genotypes Our data confirm and extend the findings of Purohit...
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Báo cáo y học: " Histone deacetylase inhibitors induce apoptosis in human eosinophils and neutrophils" docx
Ngày tải lên :
11/08/2014, 08:22
... eosinophils and neutrophils Methods Blood donors For neutrophil experiments blood was obtained from healthy donors For eosinophil experiments, blood (50100 ml) was obtained from eosinophilic individuals ... Journal of Inflammation 2010, 7:9 http://www.journal-inflammation.com/content/7/1/9 Results HDAC inhibitors enhance eosinophil apoptosis in the presence of survival-prolonging cytokines IL-5 inhibited ... than in eosinophils and the expression of HDAC8 was significantly higher in eosinophils (Figure 7) Acetylation of NF-B p65 does not explain the apoptosisinducing effect of TSA in human eosinophils...
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Báo cáo y học: " Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages" pot
Ngày tải lên :
12/08/2014, 11:22
... findings for BCG-DNA for both AM and IM, i.e high TNF-a induction and absence of IL10 induction in AM contrasting a distinct IL10 response in IM Taken together, these data obtained on mRNA level ... BCG DNA resulted in a markedly stronger TNF-a induction in AM, but an only moderate response in IM (figure 7A) Interestingly, AM completely lacked IL10 induction upon stimulation with BCG DNA, ... levels with IL10 expression in IM exceeding IL10 expression in AM 9.7-fold (± 2.4) and IL6 expression in IM being 16.9-fold (± 3.8) higher compared to AM (figure 6) These high basal expression...
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Báo cáo y học: "Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage" potx
Ngày tải lên :
12/08/2014, 15:23
... opportunities concerning both matrix-based cell-containing implants and cell-free in situ regeneration Additional material Additional file 1: Additional data concerning the differentiation status of chondrocyte ... 5% carbon dioxide in serum-free DMEM/F12 Nutmix (DMEM/F12; Invitrogen, Karlsruhe, Germany) containing 0.1% pronase E (Sigma-Aldrich, Taufkirchen, Germany) in a spinner flask for fine mincing and ... signal-enhancing system based on biotin in terms of a clearly lower background All antibodies were diluted in PBS containing 5% bovine serum albumin The signal was developed by incubation with hydrogen...
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Báo cáo y học: "Expression of leukemia inhibitory factor (LIF) and its receptor gp190 in human liver and in cultured human liver myofibroblasts. Cloning of new isoforms of LIF mRNA" pdf
Ngày tải lên :
13/08/2014, 13:20
... expression was detected as a continuous sinusoidal staining and in the stroma of portal tracts (Fig 7A) In the cirrhotic liver, the sinusoidal staining was enhanced, whereas a very faint staining ... remains hypothetical In the case of s-LIF-D, initiation at the AUG within exon D would result in a reading-frame shift following the 6th amino-acid (aa) and a termination at aa 88, the resulting ... the C-terminal part of LIF Similar considerations apply to s-LIF-M that, in any case, does not contain an initiating AUG in exon It should be emphasized that the lack of an AUG codon does not...
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Báo cáo y học: "Genome-wide promoter extraction and analysis in human, mouse, and ra" potx
Ngày tải lên :
14/08/2014, 14:22
... promoter prediction Improving promoter prediction by incorporating both mRNA annotation and promoter conservation information We are able to combine the conservation signal in homologous promoters ... information alone (for example, PromSer, Source) To our knowledge, this is the first attempt to integrate conservation information with de novo first-exon prediction on a genome-wide scale In collaboration ... false positives when comparing using just mRNA or conservation information alone, especially when known mRNAs only have partial coding regions Furthermore, without mRNA infor- Genome Biology 2005,...
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Báo cáo sinh học: "Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells" pdf
Ngày tải lên :
14/08/2014, 19:22
... method of delivering genes Interaction between cationic lipids and DNA through ionic interaction leads to forming cationic lipoplexes [1,4] The resulting complexes fuse with the anionic surfaces ... Nikol S: Optimization of nonviral transfection: variables influencing liposome-mediated gene transfer in proliferating vs quiescent cells in culture and in vivo using a porcine restenosis model ... resuspended in medium containing 10% FCS for EC and Hanks solution for SMC 0.4 ml containing approximately × 106 cells and 20 µg CAT plasmid (1 µg/µl) was placed in a sterile electroporation cuvette...
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