mr comparison for samples prepared under different pressures

Host viral interactions host factors in coronavirus replication and coronaviral strategies of immune evasion

Ngày tải lên : 09/09/2015, 10:07

... are notorious for their propensity for host switching For instance, HCoV-OC43 bears strong resemblance to bovine coronavirus and is believed to have originated in one species before crossing ... and/or proteolytic activation, S protein undergoes extensive conformational changes to prime the virion for fusion with the host membranes The first conformation change dissociates S2 domain from ... are 3’ co-terminal The positive-sense genomic mRNA (gRNA) correspond to mRNA1 Although the mechanism is not completely understood, a three-step model for discontinuous transcription in negative-strand...

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Báo cáo y học: " Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120" docx

Ngày tải lên : 13/08/2014, 09:20

... for 30 at the indicated concentration For mAb inhibition, we used Fab fragments Percent inhibition was calculated by the formula 100 - [(t - c)/(m - c) × 100], where t represents the signal for ... the ELISA data revealed similar mAb binding patterns for full-length and variable loop-deleted forms of Fc-gp120 dimers and gp120 monomers Therefore, Fc-gp120 fusion and dimerization had little ... by CD4-IgG2 (Fig 1C), but might stem from differences in affinity of different forms of CD4 (sCD4, CD4-IgG2 and cellular CD4) for Fc-gp120 ∆V1V2V3 We next investigated other mAb inhibitors, ensuring...

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Báo cáo y học: " Correction: Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120" doc

Ngày tải lên : 13/08/2014, 09:20

... mAb concentration (µg/ml) Figure formats (closed circles) C) post-CD4 (open in the of mAbs b12, assay and and 2G12 was assessedactivity standard postCD4 B) X5,format The neutralizationcircles) ... a postCD4 assay format The neutralization activity of mAbs A) b12, B) X5, and C) 2G12 was assessed in the standard (closed circles) and post-CD4 (open circles) neutralization formats Results ... infection in the absence of mAb Results are representative of two experiments Page of (page number not for citation purposes) Retrovirology 2007, 4:23 http://www.retrovirology.com/content/4/1/23 References...

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Tài liệu Báo cáo Y học: A pool of Y2 neuropeptide Y receptors activated by modiﬁers of membrane sulfhydryl or cholesterol balance pot

Ngày tải lên : 22/02/2014, 04:20

... showed Kd of 442 ± 37 pM for control surface binding, and of 638 ± 74 pM for surface binding after pretreatment by 30 lM PAO (n ¼ 3) (B) The surface binding to dispersed rat forebrain cells after ... particulates from cells pretreated for 30 at 37 °C with 30 lM PAO Competition by nonlabeled hPYY(3–36) showed Kd of 407 ± 38 pM for control surface binding, and of 451 ± 24 pM for surface binding after ... sedimented for at 100 g to recover the cells The cells (>95% excluding Trypan blue stain) were resuspended in Opti-Mem buffer, and 200 000 cells per well were immediately plated for experimental...

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DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf

Ngày tải lên : 08/03/2014, 19:20

... cell and pre-cancerous cells undergoing malignant transformation They are not expressed in differentiated somatic cells that have been withdrawn from the cell cycle Therefore, these proteins are ... emphasized the variation in regulatory mechanisms between different developmental stages and in different cell types, underscoring the need for studies of replication control in a developmental context ... replication forks stall or collapse at DNA lesions or problematic genomic regions When a fork becomes stalled, the replisome often remains firmly associated with the fork But, when replication forks...

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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx

Ngày tải lên : 18/06/2014, 15:20

... (PBMC), culture for different time periods in sterile CO2 incubators or rapid access to highly specialized lab equipment and the use of biologicals, e.g FCS or human serum from different sources ... Page of (page number not for citation purposes) Journal of Translational Medicine 2008, 6:58 Elispot assays for the enumeration of IFN-γ or IL-2 producing cells were performed as described previously ... antigens, in the form of peptides or commercial vaccine preparations (see above) were added to 0.3 ml of heparinized peripheral blood in ml tubes Samples were then centrifuged for ten seconds...

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Báo cáo sinh học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" pptx

Ngày tải lên : 18/06/2014, 18:20

... (Fig F, G, H) samples than the 24 hr samples (Fig 1C,D,E) Specially, genome B displayed a stronger pro-apoptotic effect than genome A and C at both time points (Fig D, G) Our data therefore indicated ... revealed some clue of different pro-apoptotic property In BH3 domain, the Leu21, Leu25 and Leu28 form the hydrophobic side of the BH3 αhelix to interact with the hydrophobic cleft formed by other anti-apoptotic ... transiently transfected with HBV genome A, B, and C and incubated for 24 h (panel C, D, E, respectively) and 48 h (panel F, G, H, respectively) before collected and applied to FACS assay Cells transfected...

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Báo cáo hóa học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" docx

Ngày tải lên : 20/06/2014, 01:20

... (Fig F, G, H) samples than the 24 hr samples (Fig 1C,D,E) Specially, genome B displayed a stronger pro-apoptotic effect than genome A and C at both time points (Fig D, G) Our data therefore indicated ... revealed some clue of different pro-apoptotic property In BH3 domain, the Leu21, Leu25 and Leu28 form the hydrophobic side of the BH3 αhelix to interact with the hydrophobic cleft formed by other anti-apoptotic ... transiently transfected with HBV genome A, B, and C and incubated for 24 h (panel C, D, E, respectively) and 48 h (panel F, G, H, respectively) before collected and applied to FACS assay Cells transfected...

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Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx

Ngày tải lên : 16/03/2014, 02:20

... performed in the presence of 0.25 mm dNTPs, 1.5 mm MgCl2, 10 pmol of each primer and 0.5 U Taq polymerase, and consisted of 35 cycles of denaturation at 94 °C for 45 s, annealing at 58 °C for ... 5¢AAGACAGGGCCGTCATTATGG-3¢) All reactions were performed in triplicate and expression levels were normalized to those of 18S rRNA Real-time PCR for quantiﬁcation was performed as described above, according ... incubation, cells were ﬁxed for 15 at room temperature with 3.5% paraformaldehyde The cells were then permeabilized with 0.5% Triton X-100 and incubated with anti-Foxa2 IgG (1 : 50) for h at room temperature...

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Báo cáo khoa học: In vitro embryonic developmental phosphorylation of the cellular nucleic acid binding protein by cAMP-dependent protein kinase, and its relevance for biochemical activities pdf

Ngày tải lên : 16/03/2014, 12:20

... effect on rp-mRNA translation [38] According with our data, CNBP phosphorylation status would not be a determinant for 5¢ TOP-mRNAs binding, but might affect the stability of mRNA conformation through ... zinc-ﬁnger protein CNBP is required for forebrain formation in the mouse Development 130, 1367–1379 Abe Y, Chen W, Huang W, Nishino M & Li YP (2006) CNBP regulates forebrain formation at organogenesis ... involving CNBP has been proposed for 5¢ TOP-mRNA translational control in which the 5¢ UTR is in equilibrium between open and closed conformations A closed CNBP-bound form would result in translation...

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Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebraﬁsh genome by subfunctionalization of tissue-speciﬁc expression doc

Ngày tải lên : 16/03/2014, 22:20

... to the cDNA for crabp1a and crabp1b A RT-PCR product corresponding to the constitutively expressed rack1 mRNA coding for receptor for activated C kinase was generated from RNA in all samples A ... placode (Fig 4A1–3) At 24 hpf, crabp1a mRNA was first detected in the neurohypophysis and the epidermis of the tail bud (Fig 4B1–3) In comparison, crabp1b mRNA-specific hybridization signal was ... heart, muscle and testis crabp1b mRNA was detected by RT-PCR in the skin, intestine, brain and muscle of adult zebrafish As a positive control for RT-PCR, receptor for activated C kinase gene (rack1)-specific...

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Báo cáo Y học: Irregular spiking in free calcium concentration in single, human platelets Regulation by modulation of the inositol trisphosphate receptors ppt

Ngày tải lên : 17/03/2014, 17:20

... thimerosal (TMS) under conditions, as described for Fig Manoalide (10 lM) was added at either before (A) or 1.5 after (B) thimerosal In other experiments, PGE1 (10 lM) was added at before (C) or 1.5 ... single platelets, and are representative for > 50 cells through the InsP3 receptor channels Therefore, platelets in suspension were permeabilized with saponin under low Ca2+-buffering and ATP-regenerating ... single platelets, representative for 50–100 cells from at least four independent experiments 136 mM NaCl, 20 mM glucose, mM Hepes, mM KCl, mM MgCl2 and 0.1 mM EGTA prepared in calcium-free water...

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Báo cáo khoa học: [Na+]i-induced c-Fos expression is not mediated by activation of the 5¢-promoter containing known transcriptional elements ppt

Ngày tải lên : 30/03/2014, 08:20

... Effect of ouabain on intracellular exchangeable Na+ (A) and c-Fos mRNA (B) in different cell types Serum-deprived cells were incubated for h in DMEM ± 10 lM (HeLa, C7-MDCK and C11-MDCK cells) or mM ... - + - + + + Fig c-Fos mRNA expression in HeLa cells (A) Kinetics of c-Fos mRNA accumulation in serum-deprived cells incubated in DMEM containing 10 lM ouabain (B) c-Fos mRNA in HeLa cells after ... treated with lM ouabain for up to h (B, C) Effect of the ERK kinase inhibitor PD98059 on c-Fos expression in control cells and in cells treated for 30 with 5% fetal bovine serum or for h with lM ouabain...

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Báo cáo khoa học: Binding areas of urokinase-type plasminogen activator– plasminogen activator inhibitor-1 complex for endocytosis receptors of the low-density lipoprotein receptor family, determined by site-directed mutagenesis doc

Ngày tải lên : 30/03/2014, 10:20

... amino acids is required for this common structure, with 28 nonconserved positions Receptors from the LDLR family alone accounts for 132 different CTRs Crystallographic and NMR studies of individual ... Signicantly different from binding observed with wild-type uPAwild-type PAI-1 complex (P < 0.01) b Values for the complex with mutations in both uPA and PAI-1, which are signicantly different from ... cells were allowed to bind the respective uPAPAI-1 mutant complexes for h on ice and subsequently washed before incubation for at either or 37 C Cell surface-associated complex was then released...

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báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx

Ngày tải lên : 19/06/2014, 22:20

... CCR1, CCR2 and CCR5 mRNA are visualized by dark field illumination of the photo emulsion-dipped slides Intensive signals for CCR1 and CCR5 mRNA, and moderate signals for CCR2 mRNA, were detected ... Distribution of CCR1, CCR2, CCR5 mRNA expressing cells at different time points in the spinal cord of MOG-EAE rats Figure Distribution of CCR1, CCR2, CCR5 mRNA expressing cells at different time points ... kinetic analyses were performed Rats (n = 3/time-point) which conformed in the clinical score curve were chosen for histopathology and evaluation of CCR1, CCR2 and CCR5 mRNA expression in the spinal...

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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf

Ngày tải lên : 19/06/2014, 22:20

... UCM-707 (3 mg/kg) or the corresponding vehicle (n = for each group) immediately after virus infection for three consecutive days Analysis were performed using representative microphotographs of coronal ... (A) or Cnr1-/- TMEV-infected mice (B), stained for CD11b with Iba-1 antibody and counterstained with toluidine blue (n = for each group) To perform the analysis of microglia phenotype morphology ... mg/kg) or the corresponding vehicle (n = for each group) immediately after the virus infection for three consecutive days (A) Coronal brain section level for the analysis of CD11b+ expression (B)...

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báo cáo hóa học: " Glrt-Based Array Receivers for The Detection of a Known Signal with Unknown Parameters Corrupted by " docx

Ngày tải lên : 21/06/2014, 00:20

... purpose of this paper is to introduce and to analyze the performance of different array receivers for the detection of a known signal, with different sets of unknown parameters, corrupted by an unknown ... in the military domain For example for the standardised S-mode of such systems, the signal transmitted by a target for its identification is a PPM signal which has the form (1) where v(t) is ... not only for radar applications but also for synchronization or time acquisition purposes in radio communications or in GNSS systems Indeed, for such applications, the problem may be formulated...

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Báo cáo hóa học: " Research Article Optimal Design of Nonuniform Linear Arrays in Cellular Systems by Out-of-Cell Interference Minimization" ppt

Ngày tải lên : 22/06/2014, 19:20

... from uniform ( f (ϑ) = 1/(2π) for κ = 0) to a Dirac delta around the main direction of the cluster ϑ = (for κ → ∞) Therefore, by appropriately adjusting parameter κ and the angular spreads for each ... [ρi(1) , ρi(2) ] vectors For instance, for a uniform distribution of the scatterers along the ring (i.e., κ = 0), it can be easily proved that the optimal rank-2 approximation (for i = 0, , M) results ... spacings for the line-of-sight scenario (24) form a grid (see Figure 5(a)) that contains the optimal spacings for the previous case in Figure with larger angular spread Notice that, for every...

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The rheumatoid arthritis shared epitope increases cellular susceptibility to oxidative stress by antagonizing an adenosine-mediated anti-oxidative pathway ppt

Ngày tải lên : 09/08/2014, 10:20

... electrodes Electrophoresis was performed at V/cm for 10 minutes At the end of electrophoresis, samples were fixed in 100% methanol for minutes and 100% ethanol for minutes After drying, 50 μl of ... performed for minutes at 32°C To stop the reaction, μl of 0.5 M EDTA (pH 8.0) was added, followed by boiling for 10 minutes cAMP generation in the reaction, rep- Page of 12 (page number not for ... contributions SL performed signal transduction experiments and analyzed the data ZL performed some of the experiments involving cAMP and PKA signaling OB prepared recombinant HBc particles LX performed PKA...

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