mp4 a human hemoglobin modified with maleimide polyethylene glycol

... a- amino-b-carboxymuconate-e-semialdehyde decarboxylase J Am Chem Soc 129, 9278–9279 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada H & Shibata K (2002) Identification and expression of a cDNA encoding human 6622 ... a- amino-b-carboxymuconate-e-semialdehyde decarboxylase (ACMSD) J Biol Chem 277, 35162–35167 Tanabe A, Egashira Y, Fukuoka SI, Shibata K & Sanada H (2002) Expression of rat hepatic 2-amino3-carboxymuconate-6-semialdehyde ... Cimadamore F, Orsomando G & Raffaelli N (2007) Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism...

Ngày tải lên: 18/02/2014, 06:20

... proteins, particularly when producing larger Hb conjugates As indicated earlier, catalase is a key component when generating functional Hb-based blood substitutes We have prepared an SOD–catalase fusion ... 906–912 Alagic A, Koprianiuk A & Kluger R (2005) Hemoglobin superoxide dismutase – chemical linkages that create a dual-function protein J Am Chem Soc 127, 8036–8043 Tarasov E, Blaszak MM, LaMarre ... in addition to cellular damage such as lipid peroxidation, protein oxidation, and DNA damage [21,22] Hb may generate reactive oxygen species, which can be particularly deleterious in clinical applications,...

Ngày tải lên: 07/03/2014, 00:20

... Shikama, J (1997) Biphasic nature in the autoxidation reaction of human oxyhemoglobin Biochim Biophys Acta 1337, 96–104 22 Tsuruga, M., Matsuoka, A. , Hachimori, A. , Sugawara, Y & Shikama, K (1998) ... pH range from 5.3 to Tsuruga and Shikama [21] confirmed that the fast phase of oxidation was due to the a chains and the slow phase was due to the b chains Tsuruga et al found that the beta chain ... a water molecule which can then accelerate the displacement of the protonated superoxide anion, as was suggested by Tsuruga and Shikama [21] to explain the increase in oxidation rate of the a...

Ngày tải lên: 08/03/2014, 10:20

... I-related receptor consisting of a heavy chain (HC) with three ectodomains (a1 , a2 and a3 ), a transmembrane part and a short intracellular signaling tail Like MHC class I HC, the FcRn counterpart ... Sundaresan G, Subbarayan M, Carter NH, Ikle DN, Yazaki PJ, Chatziioannou AF, Gambhir SS et al (2005) Tailoring the pharmacokinetics and positron emission tomography imaging properties of anti-carcinoembryonic ... The Authors Journal compilation ª 2008 FEBS 4103 A B A4 05 J T Andersen et al A4 05 FcRn HC mutant D A 405 C Fig Immunization, purification and evaluation of anti-FcRn antibody preparations Goat...

Ngày tải lên: 16/03/2014, 06:20

... DAnorm ¼ aa Á expðÀka Á ½O2 Š Á tÞ þ ab ÁexpðÀkb Á ½O2 ŠÁtÞ ð2Þ where DAnorm is a normalized change in optical density of the sample and aa, ab, k a and k¢b are the amplitudes and rate constants ... R, Khan I, Juczszak L, Wang J, Manjula B, Acharya SA, Bonaventura C & Friedman JM (2004) Domain-specific effector interactions within the central cavity of human adult hemoglobin in solution and ... The authors thank Anna V Chistyakova and Dr Nona V Konovalova for preparing protein solutions This work was supported by the Belarusian Republican Foundation for Fundamental Research (Grant B00-176)...

Ngày tải lên: 16/03/2014, 14:20

... (Hsp9 0a, forward primer GCTTGAAGCAAGCCTCGATGCCT GAGGAAACCCAGACCCAA, reverse primer CAGT AGCTTCATCTTTTCGGTCTACTTCTTCCATGCGTGA; Hsp90b, forward primer GCTTGAAGCAAGCCTCGAT GCCTGAGGAAGTGCACCATGGA, reverse ... PCR amplification of the Hsp9 0a ORF using the forward primer AAATAAGTCG ACATGCCTGAGGAAACCCAG (SalI site underlined; Hsp9 0a start codon in bold) and the reverse primer CTTC ATCTGCAGTTAGTCTACTTCTTCCAT ... kinase-5 (ERK5) mitogen-activated protein (MAP) kinase [18] GR assays indicated that human Hsp9 0a and Hsp90b, as well as the native yeast Hsp90s, were all capable of activating GR in these strains...

Ngày tải lên: 23/03/2014, 07:20

... graded transactivation potential Nucleic Acids Res 25, 2723–2729 13 Akagi K, Kanai M, Saya H, Kozu T & Berns A (2001) A novel tetracycline-dependent transactivator with E2F4 transcriptional activation ... CGGGCCCGGGATCCATC gccacc ATGG TGA; the 3¢ interface is CAAGTAAA GCGGCCGC For pEBTetD ⁄ eGFP, the 5¢ interface is identical; the 3¢ interface is CAAGTAAA GCGGCCGCGG Cell culture, transfection, and flow ... and with a GAPDH probe (cf Fig 2) Relative background transcription was calculated from the ratios of signals for transporter mRNA and GAPDH mRNA With OCT1, OCT2, and CAT4 both vectors were analyzed...

Ngày tải lên: 23/03/2014, 09:21

... Tsuruga, M., Matsuoka, A. , Hachimori, A. , Sugawara, Y & Shikama, K (1998) The molecular mechanism of autoxidation for human oxyhemoglobin: tilting of the distal histidine causes nonequivalent oxidation ... the stability of human HbO2, we have used this time the valency hybrid tetramers As a result, the b chain was found to acquire a noticeable resistance against the acidic autoxidation in a manner ... EDTA at 35 °C In this tetramer, even if freshly prepared, the a- peak (at 577 nm) was always lower than the b-peak (at 541 nm) with an absorbance ratio of a/ b ˆ 0.90, this being in contrast to a...

Ngày tải lên: 31/03/2014, 15:20

... chair could have Realism with a Human Face 27 been in a different place, as we say, what that means is that a counterpart of this chair could have been in that place; not that this very chair ... conclude that L) has a meta-language ML) L, has a meta-language ML z in just the way that anyone who accepts "All men are mortal" is immediately able to conclude (given the additional premise that Tom, ... Utopian; we can learn a great deal from partial and even fragmentary reconstructions, and we can learn a great deal from reconstructing our beliefs in alternative ways "Delicate mutual adjustment"...

Ngày tải lên: 11/06/2014, 13:16

... Reduced balance sheet intensity > No Project Finance Loan Arranger, Asia Pacific, (Dealogic Capital Data Project Ware) > No Project Finance Loan Arranger, Asia, (Dealogic Capital Data Project Ware) ... Singapore, Taiwan, Thailand and Vietnam Pacific American Samoa, Cook Islands, East Timor, Fiji, Papua New Guinea, Samoa, Solomon Islands, Tonga and Vanuatu Ian Richards Head of Strategy Future Objectives ... endeavour and has already contributed $750,000 to the appeal ANZ and the Environment ANZ realises that it cannot separate its financial operations from the environmental impact and has appreciated...

Ngày tải lên: 04/07/2014, 22:17

... for A5 9V, A5 9V-R (5’-CCTCAAAGTTCTCAGTAACGTCACCTCCAGCTTG-3’) and A5 9V-F (5’-CAA GCTGGAGGTGACGTTACTGAGAACTTTGAGG-3’); for A5 9 S, A5 9S-R (5’-CAAGCTGGAGGTGACTCTACTGAGAACTTTGAGG-3’) and A5 9S-F (5’-CA ... (5’CCAGCTTGTTCCCTGATAACTTCTTCCCCACC-3’) and L51I-F (5’-GGTGGGGAAGAAGTTATCAGGGAACAAGCTGG-3’); for L51T, L51T-R (5’-CCAGCTT GTTCCCTTGTAACTTCTTCCCCACC-3’) and L51T-F (5’-GGTGGGGAAGAAGTTACAAGGGAACAAGCT ... (5’-CA AGCTGGAGGTGACTCTACTGAGAACTTTGAGG3’); for G6 7A, G6 7A- R(5’-GGCATCTGTAGAGTGCGCGACATCCTCAAAGTTC-3’) and G6 7A- F (5’-GAAC TTTGAGGATGTCGCGCACTCTACAGATGCC-3’); and for G67 S, G67S-R (5’-GGCATCTGTAGAGTGCGAGACATCCTCAAAGTTC-3’)...

Ngày tải lên: 10/08/2014, 05:21

... African Americans had larger phase advances than Caucasians Because we found that African Americans had a shorter tau than Caucasians, which would augment phase advances relative to the Caucasians ... manuscript we also re-analyzed data from our previous phase-advancing study with daily light pulses [42] that included both light and dark-eyed Caucasians as well as dark-eyed African Americans, and thus ... had larger phase advances than Caucasians (n = 11) [40], suggesting that race, not iris color, was a factor mediating the magnitude of circadian phase shifts Although there are racial differences...

Ngày tải lên: 10/08/2014, 09:20

... internal standards These assay formats are usually applied, when any given concentration of a resulting outcome is assigned to a disease/health status SRM assays are also developed for relative quantitation ... health care area, both in monitoring and treating disease It is also the major disease area that requires assay-demanding activity, for clinical chemistry units at all major hospitals We have ... Extracted ion chromatograms of the Apolipoprotein assay in an LC-MS/MS analysis of pooled male (A) and female (B) plasma tryptic digest (RT: retention time, AA: peak area, using automatic integration)...

Ngày tải lên: 10/08/2014, 09:22

... Control lateral, xyphoid Cadaver 1 inferolateral Cadaver 2 lateral, xyphoid Cadaver lateral Cadaver lateral Cadaver lateral Figure Elevation and examination of deep sternal cortex and viscera McKay ... events is a complicated science Patient risk estimates are based on a number of known risk factors Cardiac and major vascular surgery places a patient at a higher risk for perioperative cardiac events ... cavity in which it was placed (Mentor Corp., Santa Barbara, CA) The manometer was connected via RS232 cable to a laptop and configured to display real-time intrathoracic pressure while simultaneously...

Ngày tải lên: 10/08/2014, 09:22

... 2) Both maxillae were hypoplastic, with three tooth anlagen on each side Between the maxillae the anlage of a single midaxial incisor was found (Fig 3a) No nasal septum or nasal cavity was present; ... nerve and the missing olfactory nerve, all other cranial nerves demonstrated a normal anatomical course (Fig 6) For comparison to normal anatomy, refer to Arnold and Kleiner [22] Cranial arteries ... was collected and alternately stained with either hematoxylin – eosin or azan Photomicrographs of every section were taken with a Nikon Coolpix 8400 camera with a resolution of megapixels In addition,...

Ngày tải lên: 11/08/2014, 20:20

... of a nymphal Amblyomma that was probably acquired at Panola Mountain State Park in Georgia in the United States of America The Panola Mountain Ehrlichia species was originally described from a ... insomnia due to pain The pain was refractory to anti-inflammatory medications, including acetaminophen, aspirin and ibuprofen Physical examination on 23 September was unremarkable Pyrexia was not ... detect antibodies against Anaplasma phagocytophilum, Borrelia burgdorferi, Coxiella burnetii, Francisella tularensis, Rickettsia africae, R akari, 'R amblyommii', R conorii, R parkeri, R prowazekii,...

Ngày tải lên: 11/08/2014, 23:21

... Talon J, Salvatore M, O'Neill RE, Nakaya Y, Zheng H, Muster T, Garcia-Sastre A, Palese P: Influenza A and B viruses expressing altered NS1 proteins: A vaccine approach Proc Natl Acad Sci USA ... www.cdc.gov/mmwr/preview/mmwrhtml/mm581 7a1 .htm] Steel J, Lowen AC, Pena L, Angel M, Solorzano A, Albrecht R, Perez DR, Garcia-Sastre A, Palese P: Live attenuated influenza viruses containing NS1 truncations as vaccine candidates against ... translation of an ORF from an influenza A virus genomic RNA, however, these data reinforce the fact that molecular processes are not perfect and that errors in transcriptional and translational...

Ngày tải lên: 12/08/2014, 04:20

... Figure Characterization of the dimerization state and splicing of viral RNA (A) Dimerization analysis of virion RNA Virion RNAs of different proviral constructs were separated on a native agarose ... compensatory mutations are uncommon in naturally occurring HIV-1 strains Furthermore, these data indicate that more than one mutational pathway can compensate for the loss of SL1 secondary RNA structure ... association with spliced HIV-1 mRNA compared to the wild type; 3- to 5-fold less HIV-1 mRNA was associated with the revertant Figure Characterization of the association between Gag and HIV-1 RNA (A) Measurement...

Ngày tải lên: 13/08/2014, 01:20

... of small uncharged residues By analogy with motifs previously characterized in Rec, a putative arginine-rich nuclear localization signal (NLS; aa 13–20) and a leucinerich nuclear export signal ... and CAT-containing cell extracts were added into microplate wells coated with anti-CAT antibody, followed by addition of a digoxigeninlabeled anti-CAT antibody, and by addition of a peroxidase-conjugated ... identical N-terminal 87 aa, whereas the C-terminal and 18 aa for SP and Rec, respectively, are unrelated in sequence (Figure 2B) for reasons described above By analogy with previously characterized...

Ngày tải lên: 13/08/2014, 05:21

... fixation with Figure Macroscopic images of the rats A: Appearance of the visceral pleura after abrasion with dry and iodinated spanch B: Placement of a x cm anti-adhesion membrane (Prevadh®) in the adhesion ... findings are also based on the result of macroscopic and histopathological examinations However, biochemical data would elucidate physiopathological changes associated with pleural adhesion and the ... Laboratory Animals Statistical analysis The results were recorded by the principal investigator and analyzed statistically upon completion of the study The statistical analysis was performed with...

Ngày tải lên: 25/10/2012, 11:00