mouse culture induced growth arrest

... Damm D, Abraham JA, Klagsbrun M, Andrews GK, Dey SK: Heparin-binding EGF-like growth factor gene is induced in the mouse uterus temporally by the blastocyst solely at the site of its apposition: ... the fact that it is required to maintain the undifferentiated proliferation of mouse embryonic stem (ES) cells in culture [6,7], LIF is not required in vivo by embryos for their normal development, ... Nevertheless, the list of genes does reveal some possible candidates that may be essential for mediating growth arrest in the implanting blastocyst With the current availability of knockout lines of mice...

Ngày tải lên: 14/08/2014, 14:21

... virus -induced inflammation in a mouse model J Dermatol Sci 2008, 52:87-97 39 Shim J, Byun HO, Lee YD, Lee ES, Sohn S: Interleukin-6 small interfering RNA improved the herpes simplex virus -induced ... Lee et al., The role of Qa-2, the functional homolog of HLA-G, in a Behcet's disease-like mouse model induced by the herpes virus simplex Journal of Inflammation 2010, 7:31 Page 12 of 12 ... glucose Qa-2 leader peptide domain siRNA did not down-regulate the Qa-2 mRNA level in in vitro PBMC cultures when compared to other domains; therefore, the leader peptide domain siRNA was injected...

Ngày tải lên: 11/08/2014, 03:20

... profiles showing the tissue culture- induced mPing activity in the three rice ssp indica culExamples Examples of transposon display (TD) profiles showing the tissue culture- induced mPing activity in ... exci- Figure of the tissue culture- induced mPing activity and alteration in cytosine methylation in the three rice ssp indica cultivars Summary Summary of the tissue culture- induced mPing activity ... culture- induced alteration in cytosine methylation at the CCGG sites of random Figure Examples of in the three rice ssp indica cultivars Examples of MSAP profiles showing the tissue culture- induced...

Ngày tải lên: 12/08/2014, 03:20

... T, Kawamoto K, Higashino K, Arita H: Prevention of growth arrest -induced cell death of vascular smooth muscle cells by a product of growth arrest- specific gene, gas6 FEBS Lett 1996, 387:78-80 ... Elevated growth- arrest- specific protein plasma levels in patients with severe sepsis Crit Care Med 2006, 34:219-222 Manfioletti G, Brancolini C, Avanzi G, Schneider C: The protein encoded by a growth ... were compared using the Mann-Whitney U test CVVH, continuous venovenous haemofiltration; Gas6, growth arrest- specific gene product tional activation from nuclear factor-κB responsive promoters [14,21],...

Ngày tải lên: 13/08/2014, 03:20

Ngày tải lên: 13/08/2014, 03:20

... by an undefined mechanism that is unrelated to Vpr -induced G2 arrest and apoptosis in mammalian cells This Vprinduced budding yeast growth arrest can be effectively inhibited by GST-fused di-W ... the interaction Vpr mutants defective for GST-B4 binding are unable to arrest yeast cell growth We next tested the growth arrest activity of these Vpr mutants in HP-16 yeast Growing yeast cells ... C-terminal arginine-rich region of Vpr is not involved in budding yeast growth arrest activity Of note, R77fs showed an impaired growth arrest activity (Fig 3, lane 17), which is most likely due to the...

Ngày tải lên: 13/08/2014, 13:20

... specifically expressed at growth arrest of mammalian cells Cell 1988, 54:787-793 Manfioletti G, Brancolini C, Avanzi G, Schneider C: The protein encoded by a growth arrest- specific gene (gas6) ... growth- arrest- specific protein plasma levels in patients with severe sepsis Crit Care Med 2006, 34:219-222 15 Gibot S, Massin F, Cravoisy A, Dupays R, Barraud D, Nace L, Bollaert PE: Growth arrest- specific ... stimulates growth of cardiac fibroblasts Biochem Biophys Res Commun 2004, 319:871-878 Hall MO, Obin MS, Heeb MJ, Burgess BL, Abrams TA: Both protein S and Gas6 stimulate outer segment phagocytosis by cultured...

Ngày tải lên: 13/08/2014, 21:21

... suppressing IC261 -induced growth arrest 16 17 Results inhibitory activity Click here data file hits cal analysis, andIC261 -induced shRNAs used Growth of for file shRNAs of all growth arrest in this ... μg/ml Culture media was replaced with virus/polybrene mix and the culture dish was incubated for h with rocking every 30 minutes After h, 10 ml of growth media was added to culture dish and the culture ... that mediates IC261 -induced growth inhibition PERIOD2 is a key substrate of CK1ε that mediates IC261 -induced growth inhibition (a) IC261, a kinase inhibitor of CK1ε, induces growth inhibition in...

Ngày tải lên: 14/08/2014, 08:21

... Experimental Study of FEIG Growth 5.1 Bridging Of Cathode Nanowire Growth 59 5.1.1 Experimental Setup 59 5.1.2 Experimental Results 61 5.2 Anode Growth 68 5.3 Simulation Of Anode Growth 72 5.3.1 Simulation ... Emission Induced Growth Formation of metallic dendritic growth at the cathode in a glow discharge in tungsten hexacarbonyl W(CO)6 ambient was first observed by Linden et al (1978) Such dendritic growth ... resistance of the anode growth due W supply-limited deposition The anode growth observed is thicker and has a tree-like structure and is deduced to arise from the continual fusing and growth of the cathode...

Ngày tải lên: 08/11/2015, 16:38

... AG1478 -induced apoptosis K Takeuchi et al kinase (ERK) ⁄ plays an essential role in EGFinduced cell growth; and the phosphatidylinositol 3-kinase (PtdIns3K) pathway is also important for cell growth ... AG1478 -induced apoptosis, we need to study whether AG1478 induces the expression of Fas or FasL in PC-9 cells Overexpression of MKP-1 inhibited the AG147 8induced JNK activation and also AG1478 -induced ... and ⁄ or PtdIns3K ⁄ Akt growth ⁄ survival signaling cascades leads to apoptosis of cancer cells However, there are no studies addressing the role of JNK in apoptosis induced by EGFR tyrosine...

Ngày tải lên: 18/02/2014, 13:20

... expresses A new method for mouse endothelial cell culture CAG loxP βgeo pA tsA58T loxP CAG pA tsA58T T26 Tg T26/Tie2-Cre Tg Tie2-Cre Tg Enzymatic digestion of organs Culture at 33 °C day tsA58T ... MAPK and Akt phosphorylation were induced in both BECs and LECs by stimulation with VEGF-A or VEGF-C, indicating that the cultured ECs responded to the endothelial growth factors Isolation and characterization ... depleted cells were cultured independently as lymphatic endothelial cells and blood vascular endothelial cells, respectively Prior to activation with growth factors, cells were cultured in endothelial...

Ngày tải lên: 18/02/2014, 17:20

... PPARb -induced keratinocyte survival transfected with an empty vector (minP-Luc2P-vector) These findings suggest that putative CERK-PPRE is the functional PPRE in mouse CERK gene keratinocyte cultures, ... for the necessity of CerK in mouse keratinocyte survival associated with activation of PPARb Cer is known to have an important role in apoptosis and cell-cycle arrest induced by various stressors ... enhances cell survival and inhibits cell death induced by serum starvation stress (A) SP1 cells under serum starvation stress treated with LD SP1 cells were cultured for an additional 24 h in the presence...

Ngày tải lên: 18/02/2014, 18:20

... several studies indicate that growth factors such as nerve growth factor, insulin-like growth factor, fibroblast growth factor, epidermal growth factor (EGF), and hepatocyte growth factor can suppress ... death induced by 10 or 20 lm ADR, whereas ngÆmL)1 EGF pretreatment markedly suppressed the cell death induced by 10 lm, ADR but not that by 20 lm ADR (Fig 1A) To evaluate whether this ADRinduced ... preventing it from exerting its proapoptotic effect on mitochondria Nerve growth factor, insulin-like growth factor, and fibroblast growth factor have been reported to transmit survival signals through...

Ngày tải lên: 19/02/2014, 06:20

... inhibits the DNA synthesis induced by VEGF165 and VEGF121 PF-4 does not affect VEGF121 -induced KDR phosphorylation We analysed the effects of PF-4 on the signalling pathways induced by VEGF165 and ... lgÆmL)1), VEGF165 -induced phosphorylation of the KDR was inhibited by 45%, whereas VEGF121 -induced phosphorylation was unaffected (Fig 2A,B) Interestingly, the level of KDR phosphorylation induced by ... ERK1/2 induced by both growth factors, VEGF165 and VEGF121, was strongly decreased by PF-4 Thus, PF-4 acts directly on or upstream from Raf1 and downstream from PLCc in the signalling cascade induced...

Ngày tải lên: 19/02/2014, 16:20

... Construction of mouse Hsp105a expression plasmid and isolation of Hsp105a-over-expressing cells Plasmid pcDNA105a was used to express mouse Hsp105a in F9 cells To construct this plasmid, the mouse Hsp105a ... determined by Western blotting using anti -mouse Hsp105 Ig (B) F9 V1, S3 and S23 cells were plated into 35 mm culture dishes at a density of · 104 cells per dish, and cultured for days at 37 °C At the ... cellular stresses and inflammatory cytokines, resulting in growth arrest and apoptosis, and have been implicated as key regulators of stress -induced apoptosis in many cell types [41,42] Furthermore,...

Ngày tải lên: 21/02/2014, 03:20

... suspension culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of ginseng cell culture Materials and methods Stock cell culture and culture condition ... plant cell cultures have been found [2,7] Optimization of the hormone concentration and combination are often effective For ginseng cell growth, 2,4 D is most commomly used in routine culture maintenance ... understand the culture characteristics of the suspended cells of ginseng in shake flask, the effect of plant growth regulators (2,4-D or IBA or NAA combination with BA or kinetin) on cell growth, ginsenoside...

Ngày tải lên: 14/03/2014, 10:20

... TSC2 have higher mTOR activity, resulting in a robust cell growth and tumorigenesis [9,10,17,18] Although it is clear that growth factorinduced activation of Akt blocks TSC1 ⁄ TSC2 inhibition of ... demonstrating how growth factor -induced PI3K ⁄ Akt activation regulates mTOR activity by modulating the interaction between TSC1 ⁄ TSC2 protein complex and Rheb Materials and methods Materials C2C12 mouse ... and Discussion IGF-1 -induced activation of mTOR signaling is prevented by PI3K inhibitor wortmannin and mTOR inhibitor rapamycin In the present study, the C2C12 cell line from mouse skeletal muscle...

Ngày tải lên: 15/03/2014, 11:20

... (days) 8 10 12 14 Fig Effects of pre -culture conditions on the heterotrophic growth of Synechocystis Growth of Synechocystis on photoautotrophic preculture (A) Growth of Synechocystis under the ... grown cultures logarithmic growth phase could grow fast heterotrophically either with or without light pulses (Fig 2B) By contrast, those harvested at the stationary growth phase of the preculture ... DNA-binding motif Results Growth of Synechocystis and its glucose utilization under heterotrophic growth conditions Photoautotrophically grown cells at the late-linear growth phase were cultured heterotrophically...

Ngày tải lên: 16/03/2014, 04:20

... Brown, C., Mendelsohn, J & Darnell, J.E Jr (1998) Epidermal growth factor -induced growth inhibition requires Stat1 activation Cell Growth Differ 9, 505–512 34 Laemmli, U.K (1970) Cleavage of structural ... Nitric oxide -induced apoptosis in human pancreatic carcinoma cell lines is associated with a G1 -arrest and an increase of the cyclin-dependent kinase inhibitor p21WAF1/CIP1 Cell Growth Differ ... Nitric oxide inhibits cell growth in cultured human thyrocytes Life Sci 59, 227–234 Sciorati, C., Nistico, G., Meldolesi, J & Clementi, E (1997) Nitric oxide effects on cell growth: GMP-dependent stimulation...

Ngày tải lên: 17/03/2014, 03:20