molecular weight mass spectrometry calculator

... chemical cross-linking and mass spectrometry. J Mass Spectrom 43, 185–195. 14 Pimenova T, Pereira CP, Schaer DJ & Zenobi R (2009) Characterization of high molecular weight multimeric states ... mass spectrometry for protein interaction analysis: application to a calmodu- lin ⁄ target peptide complex. Anal Chem 77, 495–503. 19 Tang XT, Munske GR, Siems WF & Bruce JE (2005) Mass spectrometry ... stored at )20 °C until MS analysis. High mass MALDI-TOF MS High -mass MALDI-TOF mass spectra of the intact protein complexes were obtained using a MALDI-TOF mass spec- trometer (Voyager DE STR; Applied...

Ngày tải lên: 15/03/2014, 00:20

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Ngày tải lên: 05/09/2013, 10:15

... Commun. Mass Spectrom. 2008; 22: 2993–2998 DOI: 10.1002/rcm 2994 L. Zhu et al. Real-time, on-line monitoring of organic chemical reactions using extractive electrospray ionization tandem mass spectrometry Liang ... fast response. Mass spec- trometry-based methods are of particular interest for the on- line analysis of reactions, 1 due to their high sensitivity and high specificity. Tandem mass spectrometry ... organic chemistry. 2,3 Although direct infusion electrospray ioniz- ation spectrometry (ESI-MS) 4–9 and membrane introduction mass spectrometry (MIMS) 10–12 are gaining popularity in this field, both...

Ngày tải lên: 14/02/2014, 08:20

... classification of proteins identified in CRC Proteins identified by mass spectrometry were classified by subcellular location and molecular function (Fig. 3). To better understand the probable roles ... Proteome analysis of human colon cancer by two-dimensional difference gel electrophoresis and mass spectrometry. Proteomics 4, 793–811. 5 Roblick UJ, Hirschberg D, Habermann JK, Palmberg C, Becker ... colorectal cancer by com- bining two-dimensional polyacrylamide gel electrophore- sis with a strictly mass spectrometry- based approach for data analysis. Mol Cell Proteomics 5, 2092–2101. 9 Kim H, Kang...

Ngày tải lên: 16/02/2014, 15:20

... peptide mass fingerprint from MALDI-TOF MS showed m ⁄ z values corres- ponding to all eight theoretical peptides plus the peptide of the truncated protein (residues 113, TPDVSSALDKLKE, theoretical mass ... reflectron mode and in a QSTAR o-MALDI mass spectrometer (Applied Biosystems Inc., Bellarica, CA, USA). MS ⁄ MS analysis was conducted with the QSTAR o-MALDI mass spectrometer. The TOF region acceleration ... National Hemophilia Clinical Fellow- ship Award. KM is a Bush Medical Fellow (Bush Foundation). The mass spectrometry and parts of the gel electrophoresis were conducted with the assistance of Mr Thomas...

Ngày tải lên: 19/02/2014, 05:20

... by MALDI-TOF MS. The measured molecular mass of the purified product was 11 799.70 ± 2, which concurs closely with activated (Pyr)-ONC (M23L), which has a theoretical molecular mass of 11 801.60. Contribution ... intensities of the peaks corresponding to Gln-ONC (M23L) (theoretical molecular mass 11 818.87) and Pyr-ONC (M23L) (theoretical molecular mass 11 801.60), and expressing it as the fraction of hydrolyzed (Met1)-ONC ... simple system constituted by only three molecular species, each very similar in net charge, surface charge distribution, hydrophobicity and molecular mass, it is worthwhile to assume that their...

Ngày tải lên: 19/02/2014, 12:20

... oligosaccharides by com- bined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry. J. Am. Soc. Mass Spectrom. 13, 670–679. 15. Domon, B. & Costello, ... (see below), having the same molecular mass with one Fuc in each of the 3- and 6-branches. Comparison of the product-ion spectra of the singly and doubly charged molecular ion precursor (Fig. ... contaminant in DFiLNO as the precursor (B). 1176 H. Kogelberg et al.(Eur. J. Biochem. 271) Ó FEBS 2004 mass spectrometry and 1 H-/ 13 C-nuclear magnetic resonance spectroscopy. Evidence for a new core...

Ngày tải lên: 19/02/2014, 12:20

... stereospecificity at C5 of coenzyme F 420 for F 420 H 2 oxidase from methanogenic Archaea as determined by mass spectrometry Henning Seedorf 1 ,Jo ă rg Kahnt 1 , Antonio J. Pierik 2 and Rudolf K. Thauer 1 1 ... nicotinamide adenine dinucleotide-linked oxidoreductases: electron impact and field desorption mass spectrometry. Anal Biochem 101, 413–420. 22 Warkentin E, Mamat B, Sordel-Klippert M, Wicke M, Thauer ... (5R)- [5- 2 H 1 ]F 420 H 2 to [5- 2 H]F 420 as to be expected for a Si-face-specific enzyme. The finding that the 775 Da mass peak in Fig. 2Cb was much lower than in Fig. 2Bb is probably due to the fact that reduction of...

Ngày tải lên: 20/02/2014, 03:20

... Techniques for the Analysis of Organic Chemicals by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Petrochemical Authors Ed McCurdy & Don Potter Agilent Technologies ... Cheadle Royal Business Park Manchester, SK8 3GR UK Abstract Inductively Coupled Plasma Mass Spectrometry (ICP- MS) is used for the routine monitoring of trace and ultra-trace metal contaminants ... technique is available to any Agilent ICP-MS system fitted with the ShieldTorch System and a mass flow controller capable of adding oxygen to the plasma. In routine operation, automatic switching...

Ngày tải lên: 05/03/2014, 21:20

... soft ionization mass spectrometry. Int J Mass Spectrom 216, 1–27. 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein complexes by mass spectrometry. Mass Spectrom Rev 23, ... (1998) Fourier transform ion cyclotron resonance mass spec- trometry: a primer. Mass Spectrom Rev 17, 1–35. 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron ... interconversion of the two most common frequency-to -mass calibration functions for Fourier transform ion cyclotron resonance mass spectrometry. Int J Mass Spectrom 195 ⁄ 196, 591– 598. 83 Otwinowski...

Ngày tải lên: 07/03/2014, 17:20

... least reliable mass isotopomer fractions were considered in the flux analysis. Discussion In the work described, we examined the extent to which mass isotopomer measurements of biomass hydrolysates, after ... time profiles of the (M + 0) and (M + 1) mass isotopomer fractions of selected tributyl dimethyl silyl (TBDMS)-amino acid fragments. M depicts the molecular weight of a fragment, i.e. all its atoms ... and PEP Fig. 3. Time profiles of the M + 0 and M + 1 mass isotopomer fractions of selected TBDMS-amino acid fragments. Mdenotesthe molecular weight of a fragment, i.e. all its atoms are in their...

Ngày tải lên: 08/03/2014, 08:20

... low molecular weight phosphotyrosine- protein phosphatase. J Biol Chem 271, 1278–1281. 10 Tailor P, Gilman J, Williams S, Couture C & Mustelin T (1997) Regulation of the low molecular weight phosphotyrosine ... activation of a low -molecular weight protein tyrosine phosphatase by adenine. Biochemistry 39, 1234–1242. 39 Xu H, Xia B & Jin C (2006) Solution structure of a low -molecular- weight protein tyrosine ... [5], has also been Keywords low -molecular- weight protein tyrosine phosphatase (lmwPTP); phosphatase regulation; protein oligomerization; signaling pathways; supramolecular proenzyme Correspondence M....

Ngày tải lên: 16/03/2014, 02:20

... Microbiologı ´ a Molecular, Madrid, Spain 2 Bijvoet Center for Biomolecular Research, Department of NMR Spectroscopy, Utrecht University, The Netherlands 3 Bijvoet Center for Biomolecular Research, Biomolecular ... nanoflow electrospray ionization orthogonal time-of-flight mass spectrometer (Micromass LC-T; Waters, Manchester, UK) modified for high mass operation and operating in positive ion mode [32]. To monitor ... analysis of macromolecular complexes. These protein complexes are detected at different mass- to-charge ratios ( m ⁄ z), separated by differences in their time-of-flight inside the mass analyser....

Ngày tải lên: 16/03/2014, 03:20

... corresponding to alternative sites of proteolysis; one fragment has a molecular mass of 32582 Da and the second has a molecular mass of 32739 Da. As expected, after incubation with methyl- malonyl-CoA, ... the ACP-TE di-domain showed two peaks, one with a molecular mass of 39506 Da corresponding to the unloaded form and the other with a molecular mass of 39604 Da, cor- responding to the form loaded ... derivative. AT ACP-TE Methyl- malonyl adduct Unloaded forms Methyl- malonyl adducts Unloaded form adduct 33 200 32 80032 400 39 500 39 600 39 70039 400 Mass (Da) Mass (Da) Fig. 3. The electrospray mass spectra pro- duced by the AT and ACP-TE fractions derived from the extension...

Ngày tải lên: 16/03/2014, 03:20

... treatment, indicating that an intramolecular disulfide bond might have formed within the loading didomain (the mass accuracy for the 56 kDa protein would not allow us to detect the 2 Da mass decrease due to ... transform mass spectrometry: The polyketide module of yersiniabactin synthetase. Biochemistry 42, 13393–13400. 17 Hicks LM, O’Connor SE, Mazur MT, Walsh CT & Kelleher NL (2004) Mass spectrometric ... identity of each fragment was obtained by mass mapping from the known amino acid sequence. Given the good mass accuracy (0.01%), for any particular observed mass a match could normally be found with a...

Ngày tải lên: 16/03/2014, 18:20

... 1254 11097-69-1 Aroclor 1260 11096-82-5 Monoisotopic molecular weight calculated from the atomic masses of the isotopes 1 with the smallest masses. Only qualitative identification of these analytes ... capillary column of a gas chromatography /mass spectrometry (GC/MS) system. Compounds eluting from the GC column are identified by comparing their measured mass spectra and retention times to reference ... and output mass spectral data. The computer software must have the capability of processing stored GC/MS data by recognizing a GC peak within any given retention time window, comparing the mass spectrum from...

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Ngày tải lên: 22/03/2014, 14:20

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