molecular pathology of cl mediated apoptosis

... feature of From: Methods in Molecular Medicine, vol 59: Molecular Pathology of the Prions Edited by: H F Baker © 2001 Humana Press Inc., Totowa, NJ Ridley this type of disease away from clinical ... does on the production of factual data Kuhn (2) put forward a view of the process of science as consisting of periods of slow accumulation of experimental results, much of which elaborates current ... a disorder of the central nervous system clinically resembling multiple sclerosis with remarkable anatomic findings (spastic pseudosclerosis) Report of a fourth case.[classical article] Alzheimer...

Ngày tải lên: 11/04/2014, 06:57

... mammalian cells Nonetheless, the precise mechanism of gene transfer is still unclear It will be necessary to identify the specific endogenous factor(s) of A tumefaciens required for the efficient mammalian ... high DNA transfer efficiency of AG6 strain might allow us to develop an efficient human cell gene delivery or a good vehicle for DNA vaccination The feasibility of using this system for DNA delivery ... in the expression of acidic pH-inducible genes, but the mechanism is still unclear Detailed studies is necessary in order to get more information for a better understanding of the signal transduction...

Ngày tải lên: 17/09/2015, 17:20

... cytoplasmic domains of other proteins In many cases (including that of EnvZ), deletion of sensor modules results in a partially or completely active form of the kinase (Parkinson and Kofoid, 1992) This ... of specific genes However, some RRs control more diverse responses such as the regulation of motility, activation of 126 mitogen-activated protein (MAP) kinase cascades, and modulation of cyclic ... including acidification and a wide rage of lethal enzymes) Such sensing and reponse could be mediated by a two-component system involved in the stress response etaRS in E faecalis was one of...

Ngày tải lên: 17/09/2015, 17:20

... tissues instead of fixed samples 108 Recently, Kunik et al (2001) have reported that the efficiency of A tumefaciensmediated stable transformation of mammalian cells is at a frequency of 10-5-10-6 ... independent of virulence genes, but the latter nuclear localization and integration steps might require the normal function of the virulence genes As we know, the mechanisms of Agrobacterium -mediated ... process and targeted to the nuclear of mammalian cells, where the foreign gene could be expressed The nuclear targeting effect of foreign gene might be due to the function of some Agrobacterium and/or...

Ngày tải lên: 17/09/2015, 17:20

... autoclaving and add 5ml of filtersterilized M MgCl2 TB 10 mM PIPS, 55 mM MnCl2, 15 mM CaCl2, 250mM KCl; LB, 500 ml; mannitol, 10 g; g sodium Cangelosi et al., glutamate, 2.32; KH2PO4, 0.5 g; NaCl, ... were resuspended in 30 ml of ice-cold TB buffer (10 mM PIPES, 55 mM MnCl2, 15 mM CaCl2, 250mM KCl, pH 6.7; all components except MnCl2 were dissolved and autoclaved; 1M MnCl2 solution was filter-sterilized ... resuspended in ml of TE buffer (10 mM Tris-HCl, 25 mM EDTA, pH 8.0) To lyse the cells, 500 µl of M NaCl, 500 µl of proteinase K (5 mg/ml), and 500 µl of 10% SDS were add to the cell suspension...

Ngày tải lên: 17/09/2015, 17:20

... consists of T-DNA, which is a single-strand DNA segment processed from Ti plasmid, a molecule of VirD2 that is an endonuclease covalently bound to the 5’ end of the T-DNA, and a large number of VirE2 ... for mediating the precise cleavage of T border sequence carried by ssDNA templates even in absence of VirD1 protein In contrast, VirD1 is essential for the cleavage of supercolied strand substrate ... efficient translation of virE2; (ii) VirE1 stabilizes VirE2 via an interaction with the N-terminus of VirE2 VirE1-VirE2 complex is composed of one molecule of VirE2 and two molecules of VirE1; and (iii)...

Ngày tải lên: 17/09/2015, 17:20

... mitochondria-dependent pathway of apoptosis, with proteins such as Bax functioning as inducers of apoptosis and proteins such as Bcl-2 functioning as suppressors of apoptosis In this regard, it ... percentage of cells showing the diffuse red fluorescence Effects of IFN-c Because the combined treatment of IFN-c and monoclonal anti-CD95 serum strongly enhanced the induction of apoptosis in ... (mainly involved in receptor -mediated apoptosis) and caspase (mainly involved in mitochondria -mediated apoptosis) were considered After 72 h of IFN-c treatment followed by monoclonal anti-CD95 serum...

Ngày tải lên: 29/03/2014, 22:21

... in vivo [8] One of the classic effects of glucocorticoids is induction of apoptosis In vitro, synthetic glucocorticoids induce apoptosis of human thymocytes and activated T cells of human peripheral ... range of 34 to 83 years) with active knee arthritis (mean duration of current knee arthritis episode of months and mean disease duration of 84 months) who fulfilled the 1987 American College of ... intraarticular injection of 40 mg of triamcinolone hexacetonide Synovial biopsy samples from areas close to cartilage were obtained prior to and a median of 11 days (range of to 14 days) after injection...

Ngày tải lên: 09/08/2014, 01:22

... in vivo [8] One of the classic effects of glucocorticoids is induction of apoptosis In vitro, synthetic glucocorticoids induce apoptosis of human thymocytes and activated T cells of human peripheral ... range of 34 to 83 years) with active knee arthritis (mean duration of current knee arthritis episode of months and mean disease duration of 84 months) who fulfilled the 1987 American College of ... intraarticular injection of 40 mg of triamcinolone hexacetonide Synovial biopsy samples from areas close to cartilage were obtained prior to and a median of 11 days (range of to 14 days) after injection...

Ngày tải lên: 09/08/2014, 13:22

... obtained by pre-incubation of His-PmrBC276 protein (5 μM) with 40 μCi of [g-32P]ATP in 80 μl of 1× phosphorylation buffer (10 mM Tris-HCl, pH 7.5; 138 mM NaCl; 2.7 mM KCl; mM MgCl2; mM DTT) for h at ... 10 mM MgCl , 0.1 mM FeCl , or 0.1 mM FeCl plus 0.3 mM ferric iron scavenger deferoxamine (SigmaAldrich) to mid-log phase (OD600 of 0.7) Then 100 μl of the culture was mixed with 900 μl of Z buffer ... NaH2PO4, 10 mM KCl, mM MgSO4, 50 mM b-mercaptoethanol), 17 μl of 0.1% SDS, and 35 μl of chloroform and the mixture was shaken vigorously After incubation at 30°C for 10 min, 200 μl of mg/ml ONPG...

Ngày tải lên: 10/08/2014, 05:21

... proposed, including decrease of cellular concentration of the drug, enhancement of drug inactivation due to increased cellular levels of metallothionine and glutathione, increase of DNA repair, ... Foundation of China and by a grant from the Scientific Research Foundation for the Returned Overseas Chinese Scholar, State Education Ministry of China Page of Author details Laboratory of Molecular ... this article as: Wang et al.: Reactive oxygen species -mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells Journal of Experimental...

Ngày tải lên: 10/08/2014, 10:20

... Furthermore, as a sign of early apoptosis, ΔΨm is also decreased after Ad-bFGF-siRNA treatment (Figure 4A) Bcl-2 and Bcl-xl counteract the proapoptotic effects of Bax and Bcl-2 antagonist killer ... Adenovirus -mediated delivery of bFGF small interfering RNA reduces STAT3 phosphorylation and induces the depolarization of mitochondria and apoptosis in glioma cells U251 Journal of Experimental & Clinical ... Liu et al Journal of Experimental & Clinical Cancer Research 2011, 30:80 http://www.jeccr.com/content/30/1/80 nucleus and phosphorylates an array of downstream targets, including STAT3 [27]...

Ngày tải lên: 10/08/2014, 10:21

... extract, g; NaCl, 0.5 g; 10 ml of 250 mM KCl; pH 7.0,sterilize by autoclaving and add 5ml of filter-sterilized M MgCl2 Sambrook et al., 1989 TB 10 mM PIPS, 55 mM MnCl2, 15 mM CaCl2, 250mM KCl; Sambrook ... g; NaCl, 10 g; pH 7.5 Sambrook et al., 1989 SOC Tryptone, 20 g; yeast extract, g; NaCl, 0.5 g; 10 ml of 250 mM KCl; pH 7.0,sterilize by autoclaving and add 5ml of filter-sterilized M MgCl2; 20mM ... Agrobacterium -mediated transformation (AMT) is becoming an important modern bio-technique by the accumulation of knowledge of the molecular principles of T-DNA transfer process AMT is one of the most...

Ngày tải lên: 09/09/2015, 10:08

... assay of VirE2 in yeast cells 68 Figure 3.22 GFP11 does not perturb the function of VirE2 in AMT of plants 71 Figure 3.23 Study of putative nuclear localization signals of VirE2 in AMT of ... AND MOLECULAR ANALYSIS OF YEAST FACTOR PMP3P DURING AGROBACTERIUM -MEDIATED TRANSFORMATION LI XIAOYANG (B Sc Science.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... 16 2.2.1 Molecular cloning 16 2.2.2 Preparation of yeast genomic DNA 16 2.2.3 Preparation of A tumefaciens genomic DNA 25 2.2.4 Transformation of A tumefaciens...

Ngày tải lên: 09/09/2015, 11:19

... Physiological role of apoptosis 1.2 Mechanisms of apoptosis induction 1.2.1 Molecular players in apoptosis 1.2.1.1 Caspase family 1.2.1.2 Inhibitors of apoptosis ... is one of the earliest proteins cleaved by caspases Cleavage of ICAD allows the release and translocation of CAD into the nucleus, which results in the internucleosomal fragmentation of DNA, ... Alzheimer are thought to be the result of an excess of apoptosis Inversely, a lack of apoptosis can result in autoimmune diseases and cancer [19-21] 1.2 Mechanisms of apoptosis induction Following numerous...

Ngày tải lên: 11/09/2015, 10:14

... Death receptor mediated apoptosis requires the activation of the caspase cascade, which is responsible for the cleavage of cellular substrates to bring about the controlled demise of cells In order ... appearance of a 17 kDa cleaved fragment of caspase-3 at 8h following treatment with LY30 and TRAIL (Figure 18D), indicating an activation of pro-caspase-3 via proteolytic cleavage The caspase- 3mediated ... the actual number of colonies counted after TRAIL treatment and the apparent low number of colonies in the picture itself 85 Figure 13: Effect of LY30 treatment on TRAIL -mediated apoptosis Hela...

Ngày tải lên: 11/09/2015, 10:15

... gene silencing includes the initiation and maintenance of gene silencing The first step involves the cleavage of dsRNA into 21-25 nucleotides dsRNAs by Dicer, which is a dsRNA endonuclease (RNase ... Dicer-like enzyme (DCLs) DCL1 (originally known as: CARPEL FACTORY, SHORT INTEGUMENTS1, or SUSPENSOR1) and DCL3 accumulate mainly in the nucleus DCL1 is necessary for 12 stepwise processing of miRNAs from ... mutation of DCL1 impairs miRNA but not siRNA production (Finnegan et al., 2003), further studies of the effect of DCLs mutations on viral siRNA accumulation in A thaliana reached the conclusion...

Ngày tải lên: 16/09/2015, 08:31

... pathogen of plant Crown Gall disease In nature, its host varies in many species of the plant kingdom including more than 600 types of plant (56% of the gymnosperms and 58% of angiosperms including ... MOLECULAR ANALYSIS OF THE ROLE OF A YEAST POTASSIUM TRANSPORT COMPONENT TRK1 IN AGROBACTERIUM -MEDIATED TRANSFORMATION NGUYEN CONG HUONG (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ... PEG, stirring and top up H2O to 500ml, filter sterilized KCl 74.55g KCl, top up H2O to 1L, autoclave NaCl 292.2g NaCl, top up H2O to 1L, autoclave Lysis Buffer 1M sorbitol, 0.1M Na2EDTA, pH7.5, filter...

Ngày tải lên: 16/10/2015, 11:57

... proteins involved in apoptosis, including the expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways of apoptosis (Fig 2) ... the expression of Bcl-2 and by modulating phosphorylation of JNK In conclusion, various pro- and antiapoptosis-related proteins were expressed in the process of hypoxia-induced apoptosis in the ... expression of antiapoptotic protein Bcl-2, which would contribute to the induction of apoptosis, were detected Furthermore, increased expression of antiapoptotic proteins such as Bag-1, Hsp70, and Bcl-X,...

Ngày tải lên: 31/10/2012, 15:12

... numbers of cells with apoptosis- associated alterations of the nuclei and without membrane barrier dysfunction were determined within a field of view at a magnification of X400 A total of 10 randomly ... increased apoptosis with values of 45% and 70%, respectively These data confirm the role of G-protein coupled receptors as a regulatory mechanism of ethanol-induced apoptosis Figure Effect of inhibitors ... receptor -mediated apoptosis and the liver Journal of Hepatology 2002;37:400-10 Int J Med Sci 2006, 15 Diehl AM Cytokines and the molecular mechanisms of alcoholic liver disease Alcohol Clin Exp...

Ngày tải lên: 31/10/2012, 15:37