molecular motion and glass transition

... compilation ê 2006 FEBS R J Sola and K Griebenow Ala-Pro-Phe-pNA) Molecular modelling of the a-CT glycoconjugates coupled with molecular dynamics (MD) simulations and domain motion analysis employing ... and K Griebenow Structural dynamics and serine protease catalysis Table Kinetic and thermodynamic parameters derived from amide H D exchange rates for a-CT and for the various lactose-a-CT and ... for the p-nitroanilide (pNA) substrate Glycoconjugate modelling and molecular dynamics simulations For all molecular modelling and molecular dynamics (MD) simulation experiments the yasara suite...

Ngày tải lên: 19/02/2014, 05:20

... Semchyshyn and Volodymyr I Lushchak Chapter Oxidative and Nitrosative Stresses: Their Role in Health and Disease in Man and Birds 47 Hillar Klandorf and Knox Van Dyke Chapter Nitric Oxide Synthase and ... MOLECULAR MECHANISMS AND BIOLOGICAL EFFECTS Edited by Volodymyr Lushchak and Halyna M Semchyshyn Oxidative Stress Molecular Mechanisms and Biological Effects Edited by Volodymyr Lushchak and ... Akar and Olga Bỹyỹkleblebici Section Cellular and Molecular Targets Chapter 10 155 + + Renal Redox Balance and Na , K -ATPase Regulation: Role in Physiology and Pathophysiology Elisabete Silva and...

Ngày tải lên: 12/02/2014, 16:20

... (Fig 1D) GmPDIL-3a and GmPDIL-3b have two predicted thioredoxin domains between amino acids 65–164 and 403–481, and 68–167 and 406–508, respectively, corresponding to the a-domain and a¢-domain of ... (Fig 3A, lanes and 5) Anti-GmPDIL-3a serum reacted with both a 55 kDa and a 59 kDa band in western blot analysis of cotyledon proteins (Fig 3A, lane 3) Both the 55 and 59 kDa bands were N-glycosylated, ... left, and density (gÆmL)1) is indicated on the top (B) Microsomes were treated without (lanes and 2) or with (lanes and 4) proteinase K, in the absence (lanes and 3) or presence (lanes and 4)...

Ngày tải lên: 18/02/2014, 11:20

... 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... prim–pol domain S Prato et al Zn-binding motif and the two disulfide bonds respectively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in ... truncated proteins SDS ⁄ PAGE of purified Rep245 and Rep516 proteins Lane M, molecular mass markers; lane and 2, purified C-His6-tagged Rep516 (59 kDa) and Rep245 (29 kDa), respectively The pH dependence...

Ngày tải lên: 18/02/2014, 18:20

... subunit The FP band is not very clear in the agarose gel, and the longest band was used to run another PCR reaction for amplification Experimental procedures Extraction, purification and crystallization ... dyed with Coomassie blue G250, and the four bands corresponding to subunits of porcine complex II were cut out and N-terminal sequenced by the Edman degradation method and amino acid analyzer in ... modulate the interaction between membrane protein and detergent Results and Discussion The mitochondrial respiratory complex II preparation was extracted and purified from porcine heart The major purification...

Ngày tải lên: 19/02/2014, 02:20

... health and biodiversity, including impacts on forests, grasslands, and wetlands; and • The growth and use of cultivated invasive or noxious plants and their impacts on the environment and agriculture ... of EISA and EPACT and evolving federal and state renewable fuel standards provide some assurances that there will, in fact, be a demand for both corn-based ethanol and advanced biofuels and create ... Metrics and Standards  Many participants stressed the need for clearly stated policy goals and performance-based standards, and the value of developing standards which are flexible and adaptable...

Ngày tải lên: 19/02/2014, 09:20

... degenerated PCRs and RACE, we obtained full length preprotachykinin cDNA sequences for L maderae and P americana The cDNAs of 1512 bp 3366 R Predel et al (lem, GenBank accession number AY766011) and 1200 ... TKRPs are identical in L maderae and P americana, only six amino acid substitutions and one deletion were found (Fig 1) The signal peptide sequence (69.2% identity) and the spacer sequences between ... TKRP-2 and -3 In addition, distinct mass signals typical of unprocessed TKRP-2 and TKRP-3 were observed All TKRPs 3368 typical of P americana (PeaTKRP-3,4,6,9,10,14) were subsequently chosen for tandem...

Ngày tải lên: 20/02/2014, 01:20

... some sera to a 9- and a 15-kDa band We could show that the 9-kDa band in the tomato extracts reacts with a specific antibody against the LTP from cherry, Pru av and the 15-kDa band shows reactivity ... elution fractions 30–33 Lyc e with a molecular mass of 50 kDa was eluted, fractions 34–37 contained a band of 36 kDa and the fractions 38–41 a protein with a molecular mass of about 20 kDa (Fig ... Karlsruhe, Germany) and proteins were separated by SDS/ PAGE using a 10% resolving gel with 1.5-mm spacers Desired bands were excised from the gel after staining with 0.3 M CuCl2 and the protein...

Ngày tải lên: 21/02/2014, 00:20

... Molecular Nutrition and Genomics Molecular Nutrition and Genomics Nutrition and the Ascent of Humankind Mark Lucock BSc(Hons), PhD, MRCPath, ... from molecular nutrition, nutritional sciences, and nutrition and dietetics to anthropology ACKNOWLEDGMENTS Given the challenging workload of today’s university academic, and the time demands ... The bases face inward and stabilize the double helix via hydrogen bonds between adjacent T and A bases, and again between adjacent G and C bases This base pairing is specific, and purine always interacts...

Ngày tải lên: 06/03/2014, 05:21

... neutral and acidic pH conditions by a variety of spectroscopic (i.e CD, fluorescence and second-derivative UV absorption) and biochemical (i.e limited proteolysis and ligand binding) techniques, and ... comparative analysis of Ngb and Mb may be used to better define the relationships between these two globins and to obtain a better understanding of the molecular features and biological function of ... ligands, both the ferrous and ferric forms of Ngb are hexacoordinated, with the proximal His64 being the endogenous ligand Therefore, Ngb ligand binding requires the displacement of the intramolecular...

Ngày tải lên: 07/03/2014, 02:20

... Fig Molecular forms of Scpep1 (A) Analysis of molecular forms of Scpep1: 100 lg of cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 were separated by SDS-PAGE, blotted and ... high transcript levels in kidney and aorta in rat and lower levels in heart, spleen and lung, whereas the human transcript was detected strongly in the kidney and heart but at a low level in a ... transferase) and urine parameters showed no pathological findings The activities of several lysosomal hydrolases were normal in various tissues and lysosomes from liver and kidney of Scpep1-gt mice, and...

Ngày tải lên: 07/03/2014, 03:20

... and nitrogen for germination and early growth [18] Primary soybean seed storage proteins are globulins called glycinin and b-conglycinin They are folded and assemble into trimers in the ER, and ... between a and b and b¢ and a¢ (Fig 1C) In the case of GmPDIL-2, Lys175, Glu43, Glu62, Glu68 and Glu135 were identified by N-terminal sequencing of the enzymatic digest fragments, respectively, and Glu541 ... b and b¢ and b¢ and a¢ in GmPDIL-2, the structure of these regions may be protease resistant The activity of recombinant GmPDIL-1 and GmPDIL-2, which catalyze oxidative refolding of reduced and...

Ngày tải lên: 07/03/2014, 05:20

... japonica CYP719 was renamed CYP719A1, and EcCYPA and EcCYPB were designated CYP719A3 and CYP719A2, respectively Sequence analysis also showed that CYP719A2 and CYP719A3 had conserved eukaryotic ... examined whether CYP719A2 and CYP719A3 could function as CHS or STS Heterologous expression of CYP719A2 and CYP719A3 in S cerevisiae and their enzymological activities CYP719A2 and CYP719A3 were heterologously ... CYP719A3 converted (R,S)-cheilanthifoline and (S)-scoulerine to stylopine and nandinine at a comparable level (Fig 6) Furthermore, when (R,S)-cheilanthifoline and (S)-THC were used as mixed substrates...

Ngày tải lên: 07/03/2014, 10:20

... intensity and exposure to solvent in a number of Trp residues (60d, 96, 148, 207, and 215) [12] In particular, Trp207 and Trp29 residues, located at the boundary between the A- and B-chains and interacting ... in vitro and its hemorrhagic phenotype in vivo Results and Discussion Effect of pH on thrombin catalytic activity and inhibition The pH-dependent steady-state amidase activities of WT and K9-deleted ... the W60d side chain (S2 site) and shifting of W215 (S3) Functional and computational data show that the catalytic cycle and efficient interaction with substrates and natural inhibitors by DK9...

Ngày tải lên: 07/03/2014, 12:20

... at days 16 and 20 and low at days and The induction of this mRNA preceded the accumulation of bryonolic acid In contrast, the level of cycloartenol synthase mRNA was high at days and and decreased ... plasmid for yeast, Kpn I and Xba I sites were introduced into the 50 and 30 -termini of the deduced ORF of LcIMS1 cDNA by PCR (25 cycles with 40 s at 94 8C, 40 s at 50 8C and at 72 8C) with Pfu ... ATTG-30 (Kpn I site underlined) and 50 -CCTCTAGAGTA AAAGTCTCCAATC-30 (Xba I site underlined) were used to modify the cDNA The PCR product was digested with Kpn I and Xba I, and ligated to the sites...

Ngày tải lên: 08/03/2014, 23:20

... agitation and aeration, and optimum conditions for xylanase activity 17,19, 21,23,24,26,38,58,82,83 there is considerable overlapping in the molecular biology and biochemistry of prokaryotic and fungal ... characteristics and regulatory relationships and the presence of a single copy of the gene and the transcript.93 Purified APX I and APX II from Aureobasidium pullulans differ in their molecular weights ... mostly by antiparrellel β strands and one short α helix and resembles a partly closed right hand.85 The differences in catalytic activities of endoxylanases of family 10 and 11 can be attributed...

Ngày tải lên: 13/03/2014, 21:56

... beating, and phylogenetic analysis shows that dynein I1 ⁄ f is highly conserved This dynein I1 ⁄ f is composed of two distinct HCs, 1a [31] and 1b [32], and three ICs, IC140, IC138 and IC97, and members ... sites for both ATP hydrolysis and microtubule binding, and a tail domain in the N-terminal, which mediates dimerization of the HCs and also provides a scaffold for ICs and light intermediate chains ... [17] and ZW10 subunit of RodZW-Zwilch [18] interact with ICs), and at least three distinct classes of light chains (LCs), namely the highly conserved LC8, and members of the roadblock ⁄ LC7 and...

Ngày tải lên: 14/03/2014, 22:20

... ‘open’ (standard conformation; PDB 1YC0) and ‘closed’ (nonstandard conformation; PDB 1YBW) HGFA forms are superimposed, with the two different 220-loop conformations shown in cyan and magenta, ... intermain chain hydrogen bonds between P1 Arg and Ser214 and between P3 Gln and Gly216 (Fig 3A) S1 is filled with the P1 Arg, which engages in standard salt bridge interactions with HGFA Asp189, ... specificity-determining loops (chymotrypsinogen numbering, i.e ‘38-loop’, and the corresponding Perona and Craik [6] nomenclature, i.e ‘Loop-A’) and substrate subsites (S1–S4) The catalytic triad Asp102–His57–Ser195...

Ngày tải lên: 15/03/2014, 11:20

... Ó FEBS 2003 Cloning and characterization of Na+ channel b1B (Eur J Biochem 270) 4763 reaction (RT-PCR) and RACE-PCR Marathon-ReadyTM human adrenal gland and fetal brain cDNA libraries ... denaturation at 94 °C for 30 s; five cycles of 94 °C for s and 72 °C for min; five cycles of 94 °C for s and 70 °C for min; and 20 cycles of 94 °C for s and 68 °C for The RACE–PCR product was then cloned ... GAGGCTGCTGGCCTTAGTGGTC-3¢) and SB1-19 primer (5¢-GTGTGCCTGCAGCTGCTCAA-3¢), and lL of 50· HF2 DNA polymerase mixture (Clontech) Four independent clones were selected and subjected to double stranded DNA sequencing...

Ngày tải lên: 16/03/2014, 23:20

... mutations, overexpression of receptor ligands, and/ or the loss of negative regulatory pathways The overexpression of EGFR and its ligands (EGF and others) and/ or the loss of the mechanisms that ... SPINK1 and PRSS1 genes, with a high risk of pancreatic cancer For this population, the cumulative risks of pancreatic cancer at the age of 50 and 75 years are 11% and 49% for men and 8% and 55% ... Lankisch, P.G., Andersen, J.R., Dimagno, E.P., Andren-Sandberg ,A., &Domellof, L (1993) Pancreatitis and the risk of pancreatic cancer International Pancreatitis Study Group New England Journal of...

Ngày tải lên: 17/03/2014, 00:20