molecular markers and identification of cultivar or breed

... (Figure 4) and an MP tree of 75,521 steps and 14,974 parsimony informative characters The MP tree had a a consistency index (excluding uninformative characters) of 0.3649 and a retention index of 0.5997 ... phylogenetic analysis, correlation is dependent on the length and properties of DNA or amino acid information Because the information on orchid cpDNA is limited, the combination of several sequences ... for drawing the map in Figure This work was supported by the Development Program of Industrialization for Agricultural Biotechnology, Taiwan Support for MRD was also obtained from the Plant Molecular...

Ngày tải lên: 12/08/2014, 03:21

... the risk of spread of major foodborne pathogens Acknowledgments This study was supported by the Rural Development Administration, the Ministry of Agriculture and Forest, and also supported by ... for 18 h at 37oC Inhibitory zones of the growth were measured The results were interpreted by the guideline of National Committee for Clinical Laboratory Standards (NCCLS) Results Isolation of ... ATCC Cornell U Cornell U Cornell U Cornell U Cornell U Cornell U Penn Univ Penn Univ Penn Univ Penn Univ Penn Univ Penn Univ Penn Univ a Strains: A1-4 (ATCC strains), C1-6 (strains of Cornell...

Ngày tải lên: 07/08/2014, 18:21

... electrophoresis 4.2.2 Single strand DNA conformation and heteroduplexes Single strand conformation polymorphism (SSCP) is based on the specificity of folding conformation of single stranded DNA, when placed ... of markers and therefore, to achieve the same resolution power as with microsatellites or even SNPs, a higher number of markers will have to be studied Moreover, in most of the analyses performed, ... field of population genetics, and the choice of one or another has some influence on the statistical performances For instance, Cornuet et al [16], showed that genetic markers were always more efficient...

Ngày tải lên: 09/08/2014, 18:21

... Afl III and Nde I, we produced DNA fragments with predicted sizes of 0.30 kb and 0.25 kb for CAPS markers Ca-Afl0.30 and Ca-Afl-0.25, and 0.35 kb and 0.20 kb for CAPS markers Ca-Drd-0.35 and Ca-Drd-0.20 ... rather laborious and requires the use of restriction enzymes Conclusion The present study demonstrates the usefulness of the RAPD, SCAR, SNP and CAPS markers for rapid identification of variants ... Development and validation of molecular markers for characterization of Boehmeria nivea var nivea and Boehmeria nivea var tenacissima Chinese Medicine 2010 5:40 Author details Biomedical Technology and...

Ngày tải lên: 13/08/2014, 14:20

... as Indonesia and Thailand (Uchimura et al 2008) This study documents a new record for H. major in Vietnam The morphology, location, and habitats of H major are described, and a molecular phylogeny ... Gulf of Thailand and the Thai Andaman Sea Genetic diversity and population structure of H ovalis from the Western Pacific to the Western Indian Ocean Discussion New records of Halophila major for ... China Sea via the Gulf of Thailand, Andaman Sea and Bay of Bengal and role of geographic barrier based on ITS, AFLP and microsatellite analyses • To show the important role of sulfur-containing...

Ngày tải lên: 20/05/2016, 16:28

... alignment of GmPDIL-3a, GmPDIL-3b and orthologs of other plants Fig S3 Expression and CD analysis of recombinant GmPDIL-3a and GmPDIL-3b Table S1 List of primers for ATG-mutagenesis of GmPDIL-3a and ... Promotion of Basic Research Activities for Innovative Biosciences, and a Grant-in-Aid for Exploratory Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan ... were immediately frozen and stored in liquid nitrogen until use Cloning of GmPDIL-3a and GmPDIL-3b Cloning of the cDNAs for GmPDIL-3a and GmPDIL-3b was performed by 3¢-RACE and 5¢-RACE Soybean trifoliolate...

Ngày tải lên: 18/02/2014, 11:20

... sequencing and crystallization of mitochondrial complex II from porcine heart Characterization of respiratory complex II be the case that some indispensable cofactors (i.e phospholipids) for crystallization ... detergent for crystallization, and n-nonyl-b-d-maltoside as the additive Another important factor for crystallization of complex II is sucrose Sucrose was used as a stabilizer for sample storage During ... and full amino acid sequences of the porcine complex II subunits An abundance of gene sequences of mammalian complex II in the GenBank database was helpful for the design of proper primers for...

Ngày tải lên: 19/02/2014, 02:20

... of orthogonal basic vectors which can expand a multi-variate random process into a sum of products of these basic orthogonal vectors and single-variant uncorrelated random processes Let consider ... flows with separation bubble and formation of vortex shedding as well It can be predicted from the past knowledge that model B/D=1 is favorable for formation of Karman vortex shedding, where model ... 10 coordinate coordinate coordinate coordinate -5 -6 10 -1 10 10 -1 10 -1 -2 10 10 10 10 coordinate coordinate coordinate coordinate -3 10 -4 -5 -6 10 Time (s) 1.22Hz PSD 10 -10 Principal coordinates...

Ngày tải lên: 05/03/2014, 14:20

... This work was supported by a grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences and a Grant-in-Aid for Exploratory Research from the Ministry of Education, ... GmPDIL-2 and proglycinin or b-conglycinin during the course of the folding process of these proteins Results Cloning and expression of GmPDIL-1 and GmPDIL-2 To clone the soybean ortholog of Arabidopsis ... Nucleotide sequences of the ORF of both PDI genes were exactly the same as those of cDNAs cloned in this study Comparisons of soybean genomic sequences of GmPDIL-1 or GmPDIL-2 with those of Arabidopsis...

Ngày tải lên: 07/03/2014, 05:20

... using clustal w [40,41] and boxshade (http://www.ch.embnet.org/ software/BOX_form.html) Construction of S cerevisiae expression vectors The coexpression vector pGYR for P450 and S cerevisiae NADPH-P450 ... the alkaloids of Papaveraceous plants XXV Biosynthesis of the alkaloids of Corydalis incisa Pers and Chelidonium majus L incorporations of tetrahydroprotoberberines, N-methosalts of tetrahydroprotoberberines, ... Amplification of cytochrome P450 cDNA fragments from single-stranded cDNAs of cultured E californica cells Single-stranded cDNAs were synthesized from 1.4 lg of total RNA of 12-day-old cultured E californica...

Ngày tải lên: 07/03/2014, 10:20

... regulation of the isomultiflorenol synthase gene ACKNOWLEDGEMENT The authors are grateful to Dr W Kamisako (Professor Emeritus, Mukogawa Women’s University) for the gift of authentic isomultiflorenol and ... isomultiflorenol synthase, could not be identified Cloning of isomultiflorenol synthase (Eur J Biochem 268) 6315 Fig Time course of bryonolic acid accumulation and levels of isomultiflorenol synthase and ... Time course of accumulation of bryonolic acid and total sterol in cultured Luffa cells Mean of three replicates Bar indicates SD (B) Time course of levels of isomultiflorenol synthase and cycloartenol...

Ngày tải lên: 08/03/2014, 23:20

... chloroform and once with chloroform, and RNA was precipitated with 2-propanol and dissolved in RNase-free water Single-stranded RNAs were allowed to anneal by mixing equal amounts of each strand, ... including the fore gut site Recently, CHH was reported in the endocrine cells of the fore gut and hind gut of Carcinus immediately prior to and during molting, which is responsible for water uptake ... Numerous factors could influence the efficacy of interference RNA in vivo, for example, the length of target mRNA, the length and concentration of dsRNA, the region of homology between the dsRNA and the...

Ngày tải lên: 23/03/2014, 10:20

... the activity of the sodium pump and its interactions with Na+ or K+ ions A number of isoforms of the a and b subunits has been isolated from various tissues of numerous species, and it has been ... release of K+ from the E2(2K+) conformation (Fig 1, step 6) and thereby also the return of the E2(2K+) conformation to the E1ATPNa conformation The affinity of the E2(2K+) conformation for ATP, ... groups of Val304, Ala305, and Ile307 (M4) and through the side-chain oxygen atoms of Asn796 and Asp800 (M6) and Glu309 (M4) [26] A similar situation could be assumed for the coordination of cations...

Ngày tải lên: 24/03/2014, 00:21

... al.: Identification of Src-specific phosphorylation site on focal adhesion kinase: dissection of the role of Src SH2 and catalytic functions and their consequences for tumor cell behavior Cancer ... genes of a sample, queried their expression values and made the decision of "part of the class" or "outside the class" For each classifier and LOOCV iteration, the frequency of a gene (how often ... unpaired data mode of the algorithm As a cut-off for significance, an estimated FDR of 2.6% was chosen by the tuning parameter delta of the software The significance level of each gene was given...

Ngày tải lên: 18/06/2014, 15:20

... by LC-ESI-Q-TOF MS As a typical example of the kind of data used for peptide identification using MASCOT software, the tandem mass spectrum of a peptide originating from the major core protein, ... Method (LC-ESI-Q-TOF MS), and Method (MALDI-TOF/TOF MS) Identified proteins are listed according to their corresponding ORF The total number of non-redundant peptides and the percent of the protein ... which corresponds to a doubly charged ion of a peptide with molecular mass 1660.2 Da The tandem MS of the double charged ion had a good score of 62 and revealed the sequence for a peptide of the...

Ngày tải lên: 19/06/2014, 08:20

... by LC-ESI-Q-TOF MS As a typical example of the kind of data used for peptide identification using MASCOT software, the tandem mass spectrum of a peptide originating from the major core protein, ... Method (LC-ESI-Q-TOF MS), and Method (MALDI-TOF/TOF MS) Identified proteins are listed according to their corresponding ORF The total number of non-redundant peptides and the percent of the protein ... which corresponds to a doubly charged ion of a peptide with molecular mass 1660.2 Da The tandem MS of the double charged ion had a good score of 62 and revealed the sequence for a peptide of the...

Ngày tải lên: 20/06/2014, 04:20

... high molecular weight of MUC16 requires the use of extensive molecular biological approaches to study the importance of this mucin in the pathogenesis of ovarian cancer In addition, a thorough ... containing 1.5 M sodium chloride, 10 mM calcium chloride, 10 mM magnesium chloride, and 0.2% sodium azide) The column was eluted with ConA buffer and fractions were monitored for absorbance at 280 nm ... important in understanding the immunomodulatory roles of this mucin The shed Muc16 should also be found in the serum of mice bearing the Page of (page number not for citation purposes) Journal of...

Ngày tải lên: 20/06/2014, 07:20

... carry on normal activity; minor signs or symptoms of disease along a predefined study workup plan, to an endpoint of a diagnosis 80 Normal activity with effort; some signs or symptoms of disease ... standardised workup and treatment; or (C) patients whose comprehensive diagnostic workup did not reveal a diagnosis for their headache 70 Cares for self; unable to carry on normal activity or ... history and physical exam, laboratory and/ or imaging, it is reasonable to treat with analgesics, reassurance and close clinical follow up Cerebral toxoplasmosis accounted for just less than 4% of...

Ngày tải lên: 20/06/2014, 08:20

... “reversal” of the values for the T1/2 a and b values of the tumor and non-tumor bearing mice In the absence of a tumor burden, the T1/2a and b for IgG was approximately 5.2 and 3.8 times faster, ... (block*) 20 10 Organs c 30 ug % ID/g 15 ug 20 10 Organs 86 Figure Quantitation of tumor and normal organ distribution of Y-CHX-A’-panitumumab F(ab’)2 (a) Receptor-mediated uptake of 86YCHX-A"-panitumumab ... of the data reported herein SmithJones (2004) and co-workers conjugated the macrocyclic ligand DOTA to trastuzumab F(ab’)2 reporting an average of 6.3 chelates per F(ab’)2 fragment and immunoreactivity...

Ngày tải lên: 21/06/2014, 02:20

... from 95 kDa for the Jasper isolate [4] to 89 kDa for the Sp and Ab isolates of IPNV [6] The molecular weight of GC1 has been estimated as 94 kDa and has been shown to be similar to that of the Jasper ... characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus Virology 1985, 143, 35-44 Dobos P, Roberts TE The molecular biology of infectious ... frozen and thawed times and clarified by centrifugation Viral dsRNA was then extracted with phenol and chloroform, followed by digestion with proteinase K Seven primer pairs were used for reverse...

Ngày tải lên: 07/08/2014, 20:23