molecular genetics of insecticide resistance in lepidoptera

... regarding the structural integrity of the catalytic site of the protease This finding can readily be explained by the smaller size of the side chain of alanine in contrast to serine or threonine ... models The ligand binding mode of the inhibitor VX950 was investigated by computational protein-ligand docking Structural changes in the binding pocket and the catalytic triad of the protease were ... types of van der Waals interactions between V36 and F43, backbone-side chain and side chain-side chain In particular, F43 is directly involved in forming the hydrophobic cavity and in interactions...

Ngày tải lên: 14/08/2014, 08:20

... saquinavir, indinavir, dexamethasone, aldosterone, hydrocortisone, morphine, loperamide, quinidine, digoxin, amiodarone, Cyclosporin A (low concentration), Vincristine, Vinblastine, Daunorubicin, ... non-syndromic orofacial clefting For the family based study, 150 nuclear families of single affected offspring with oral clefs were recruited from Singapore and Taiwan The phenotype of interest included ... consist of two distinct domains, the hydrophobic transmembrane domain (TMD) and the hydrophilic nucleotide-binding domain (NBD) The TMD, also known as membrane-spanning domain (MSD) or integral...

Ngày tải lên: 16/09/2015, 14:04

... accompanied by improved binding to longer ligands (up to five atoms in the main chain) and retained binding to shorter ligands [55] Of the 21 changes in the evolution of R7 from native GLYAT (Fig ... protein monomer GO catalyzes the dioxygen-dependent oxidative deamination of primary and secondary amines (sarcosine, N-ethylglycine, and glycine) and d-amino acids (d-alanine and dproline), ... GU214711.1) of 1690 bp was isolated that encodes GOX, an FAD-containing flavoprotein of 430 amino acids GOX was overexpressed in E coli, where activity in cell lysates reached 7.15 nmolÆmin)1Æmg)1 protein...

Ngày tải lên: 14/02/2014, 14:20

... The development of a pool of resistance genes and the ability of the gonococci to maintain these determinants of resistance within their genetic coding is part of the very nature of N. gonorrhoeae ... highlight the situation • maintaining a web-based system of posting treatment failures, including mapping and regularly updating the data on emerging resistant strains • collaborating with partners to ... and principles for the prevention and care of STIs, to offer guidance to ministry of health officials who have the responsibility of developing and implementing interventions for the control of...

Ngày tải lên: 18/02/2014, 15:20

... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 41 Omura, T & Sato, R (1964) Tha carbon monoxyde binding pigment of liver microsomes ... positions in CYP6A2 (helix J and vicinity of the b sheet 3–3) These results are also original because this is the first description of the role of mutations in the metabolism of an insecticide ... affecting cytochrome P450s and their relative importance in insecticide resistance From the localization of the mutations on a spatial model of CYP102A1, we also point out a new region of cytochrome...

Ngày tải lên: 19/02/2014, 12:20

... [9–13] The CCM1 gene contains 16 coding exons which encode for Krit1, a 736-amino acid protein containing three ankyrin domains and one band 4.1 ezrin radixin moesin (FERM) domain CCM2, a 10-exon gene, ... splicing has been located within the C-terminal part of the phosphotyrosin binding domain of CCM2 [12] This mutation has been shown to abolish the interaction of CCM2 and CCM1, strongly suggesting ... germline mutations in CCM lesions Based on the autosomal dominant pattern of inheritance of CCM and the presence of multiple lesions in familial CCM, contrasting with the detection of a single...

Ngày tải lên: 15/03/2014, 10:20

... possible combinations and proportions of H, R, S and E and four resistant modes, i.e mono, double, triple and quadruple resistance instead of notifying the incidence of plain resistance rates, in the ... wild strains of human type that have never come into contact with the drugs (Mitchison, 1984) Monoresistance is defined as resistance to one of the first-line drugs Any drug resistance is indicated ... indicated as total resistance for a drug with and without accompanying other drug resistance Polydrug resistance is resistance of M tuberculosis strain to two or more of the first-line drugs MDR is...

Ngày tải lên: 29/03/2014, 03:20

... mediate an interaction of PrPC with accessory proteins, such as transmembrane proteins involved in PrP-induced signaling Alternatively, deletion of the HD could indirectly affect intermolecular interactions ... or a combination of both (Fig 1) In the following, such in vivo models are described which address certain aspects of membrane topology, folding, intracellular targeting and trafficking of PrP The ... basolaterally, indicating that this domain acts as a dominant sorting signal Vice versa, Dpl or PrPC lacking the HD were found mainly at the apical surface of MDCK cells [36] An interesting activity of PrPC...

Ngày tải lên: 30/03/2014, 09:20

... antigen Insulin-like growth factor carrier protein Plasminogen Prothrombin Retinol-binding protein Testosterone~estradiol-binding globulin Thyroxine-binding globulin Transcobalamin Transferrin Low-density ... Ceruloplasmin EGF stimulation of ornithine decarboxylase Fibrinogen Gc-Globulin Haptoglobin Hepatitis B surface antigen Insulin-like carrier protein Plasminogen Retinol-binding protein Transferrin Designation ... activation of adenylate cyclase Insulin receptors Insulin activation of glycogen synthetase and stimulation of glycogenesis Interaction with human antiinsulin receptor IgG Ribonuclease II Tyrosine aminotransferase...

Ngày tải lên: 11/04/2014, 09:52

... of Translational Medicine 2008, 6:74 Introduction Every year in China, about 30,000 children, compared to 840 in UK and one of every one thousand infants in US, are born with congenital hearing ... Huang D, Han D: Molecular etiology of patients with nonsyndromic hearing loss from deaf-muta schools in 18 provinces of China Chinese Journalof Otology 2006, 4:1-5 Cohen MM, Gorlin RJ: Epidemiology, ... or in combination with an incomplete partition of the apical turn of the cochlea as part of Mondini deformity PS is differentiated from nonsyndromic hearing loss with EVA by the presence of goiter,...

Ngày tải lên: 18/06/2014, 15:20

... Neuroserpin was used as a continuous variable since there was a linearity of the odds ratios for outcome The influence of neuroserpin decrease at 24 h on serum levels of molecular markers of brain injury ... defined as neuroserpin decrease All determinations were carried out in a laboratory blind to the clinical outcome and neuroimaging findings Endpoints The primary endpoint was the decrease of ... http://www.translational-medicine.com/content/9/1/58 our group obtained after investigating neuroserpin effects using an in vitro model of brain ischemia [40] The information reported here regarding biomarkers...

Ngày tải lên: 18/06/2014, 19:20

... cassavagrowing areas of Tanzania in an effort towards identifying, determining molecular variability and mapping the distribution of CMGs In addition, because East Africa seems to be unusually rich in ... reported in East Africa, we investigated the extent of inter-CMG recombinations and examined their role in the evolution of CMGs in Africa Results Assessment of CMD symptoms Over 80% of the cassava ... mentioned in other general studies of geminivirus recombination [14] as well as in specific studies of particular groups of geminiviruses [20] Two categories of CMGs in Africa Based on recombination...

Ngày tải lên: 18/06/2014, 22:20

... Neuroserpin was used as a continuous variable since there was a linearity of the odds ratios for outcome The influence of neuroserpin decrease at 24 h on serum levels of molecular markers of brain injury ... defined as neuroserpin decrease All determinations were carried out in a laboratory blind to the clinical outcome and neuroimaging findings Endpoints The primary endpoint was the decrease of ... http://www.translational-medicine.com/content/9/1/58 our group obtained after investigating neuroserpin effects using an in vitro model of brain ischemia [40] The information reported here regarding biomarkers...

Ngày tải lên: 20/06/2014, 03:20

... cassavagrowing areas of Tanzania in an effort towards identifying, determining molecular variability and mapping the distribution of CMGs In addition, because East Africa seems to be unusually rich in ... reported in East Africa, we investigated the extent of inter-CMG recombinations and examined their role in the evolution of CMGs in Africa Results Assessment of CMD symptoms Over 80% of the cassava ... mentioned in other general studies of geminivirus recombination [14] as well as in specific studies of particular groups of geminiviruses [20] Two categories of CMGs in Africa Based on recombination...

Ngày tải lên: 20/06/2014, 04:20

... hypoxanthine-guanine phosphoribosyltransferase (hprt) locus can provide information on the mechanisms of in vivo mutation in population exposed to exogenous carcinogens and in individual with inherent ... was used in a final volume of 20 µl RT-PCR containing µl of 10×VM buffer 10 mM MgCl2 (Promega, USA), 0.4 µl of each dNTP 25 mM (Promega, USA), 0.4 ml of oligo dT (0.5 µg/µl), 1.0 µl of hprt-specific ... expected by addition of their individual responses The study of these interactions can lead to a better understanding of the toxic mechanism of the chemicals involved A number of terms have been...

Ngày tải lên: 07/08/2014, 18:20

... growth inhibition in murine cell lines [33] Recently, Taniguchi et al [34] showed that cyclin-dependent kinase inhibitor p16INK4a is induced in RA-SF by several growth-inhibited conditions, including ... protein (Fbx3) Nucleosome assembly protein 1-like Lysosome-associated membrane protein EGF-containing fibulin-like extracellular matrix protein (EFEMP1) Fibrillin Versican Laminin γ1 Fibronectin ... Discussion We analyzed the expression profile of proliferating RA-SF in comparison with that of nonproliferating RA-SF, and we observed that distinct sets of genes, including several novel genes that have...

Ngày tải lên: 09/08/2014, 06:22

... from those matings were not used for reproduction Frequencies of the different types of matings and egg-layings obtained in each generation are shown in table I Growth As room was lacking to raise ... of its offspring Since there is no contact between the layer and its offspring, it is likely that the effect is passed on through the eggs Bride and Gomot (1991) showed that, often in pairs of ... effects Further studies on inbreeding and dominance effects will require well-designed experiments such as a diallele experiment involving inbred lines obtained by successive matings between related...

Ngày tải lên: 09/08/2014, 18:22

... risk factors for developing K65R mutations among HIV-1 infected patients who fail an initial regimen of fixed-dose combination of stavudine, lamivudine, and nevirapine J Clin Virol 2008, 41:310-313 ... are running behind A random sample survey is often the only way to assess treatment efficacy and the selection of drug resistance in treatment programs in Africa The information obtained from ... recombinant of A1 and D (n = 1) No major PR resistance mutations were seen in any of the sequences, but a mean of minor PR mutations were observed per sample (data not shown) Resistance mutations in...

Ngày tải lên: 10/08/2014, 05:21

... Lager K, Huovinen P: The effect of changes in the consumption of macrolide antibiotics on erythromycin resistance in group A streptococci in Finland Finnish Study Group for Antimicrobial Resistance ... channel of such information to the public With widespread distrust of many public institutions in developing countries, partnering with media organizations to develop and probably assist in running ... combining targeted public messages, the integration of messages into popular shows and the extensive use of news media The choice of media - whether print or electronic through which to disseminate...

Ngày tải lên: 11/08/2014, 18:20

... in the risk of resistance development while on therapy [85,86] Summary Clinicians working in the ICU setting should routinely employ antibiotic strategies aimed at limiting the emergence of resistance ... against the predominant ICU pathogens, resulting in more effective treatment of nosocomial infections Antibiotic cycling is one method of achieving antimicrobial heterogeneity Other methods include ... basis of pharmacokinetic/pharmacodynamic principles could play a role in the reduction of antibiotic resistance The duration of time that the serum drug concentration remains above the minimum inhibitory...

Ngày tải lên: 12/08/2014, 22:22