molecular era of agrobacterium and crown gall research
Tài liệu Báo cáo khoa học: Molecular evolution of shark and other vertebrate DNases I pptx
Ngày tải lên :
19/02/2014, 16:20
... sequences of the purified enzymes, the mature forms of H japonicus and T scyllia DNases I were estimated to be composed of 262 and 263 residues, respectively Comparison of the primary structures of these ... activity could be detected for only the first 30 of incubation, whereas that of the human Table Summary of the purification of DNase I from g of pancreas of H japonicus Purification step Protein (mg) ... Japan, and purified the former’s enzyme The expression of a series of mutant constructs was also examined in mammalian cells, allowing several common structural and functional characteristics of shark...
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Báo cáo khoa học: Molecular mass of macromolecules and subunits and the quaternary structure of hemoglobin from the microcrustacean Daphnia magna ppt
Ngày tải lên :
16/03/2014, 13:20
... [14] gave a molecular mass of 505 ± 35 kDa in a first experiment and one of 483 ± 27 kDa in a second experiment, resulting in an overall molecular mass of 494 ± 33 kDa For the subunits of D magna ... were released by methanolysis and separated by GC followed by MS Chromatograms of the carbohydrates of D magna Hb, of the corresponding blank and of a mannose standard are shown in Fig The dominant ... system used for gel electrophoresis on the band pattern of pale and red Daphnia magna hemoglobin (Hb) The hemolymph of pale (2.0 lg of Hb) and red (2.0 lg of Hb) D magna was subjected to electrophoresis...
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Báo cáo khoa học: Molecular characterization of artemin and ferritin from Artemia franciscana pot
Ngày tải lên :
23/03/2014, 20:22
... [24–27] Helices A and B are antiparallel, as are C and D, and they are connected by small loops A large loop, designated L, connects A and B helices with C and D helices, and L loops of neighboring ... heat and chemicals such as urea and guanidinium chloride [24], and the degradation of ferritin in vivo is inhibited by excess iron [32] To address questions of structure and function, artemin and ... with a labeled artemin probe revealed bands of 2.1 and 3.7 kb, the former of expected length based on the size of cloned cDNAs The mRNA in the upper band of the blot probed with the artemin cDNA...
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Gerald Epstein Department of Economics and Political Economy Research Institute (PERI) pptx
Ngày tải lên :
29/03/2014, 13:20
... ability of countries to attract foreign investment And just as the Gold Standard operated as a "good housekeeping seal of approval" in the inter-war period of the 20's (Bordo, Edelstein and Rockoff,1999), ... high level, profit rates for both finance and industry will rise, because of asset appreciation in the case of finance and because of increased demand for products, in the case of firms However, ... Barry and Michael Bordo 2001 Crises Now and Then: What Lessons From the Last Era of Financial Globalization? University of California, Berkeley, Haas School of Business Eichengreen, Barry and Andrew...
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21st century will be an era of femininity and women can have far greater opportunity
Ngày tải lên :
08/07/2014, 21:08
... advantages of gender and out-look However out of the world’s 27 major economies, the UK is 23 rd in the number of women entrepreneurs - Women have more power ( take part in politics) • Professional and ... men and can only attend segregated schools and universities , woman witness’s testimony is given half the weight of that of a male witness, a woman can inherit only half as much as a man, and ... million skilled workers will be needed to satisfy the demands of the aerospace and transport industries Leading women speak out on election TV coverage: (from top left) Kirsty Wark, Sian Kevill, Yasmin...
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Báo cáo khoa học: "Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens" docx
Ngày tải lên :
07/08/2014, 20:23
... tnatsiser-locinehpmarolhc ytneves eht fo hcae morf AND cimoneg latot eht gnisU giF ni dedivorp era stnanimreted ecnatsiser locinehpmarolhc dna locinefrolf rof gnidocne seneg eht rof stcudorp noitacifilpma tnereffid ... remirp drawrof eht fo riap eno ,seneg Rolf eht fo noitceted eht roF ]5[ debircsed ylsuoiverp sa deraperp erew etalosi hcae morf AND latot fo setalpmeT RCP aiv detceted erew )3-tac dna ,2-tac ,1-tac ... elbaT( )lm/gµ 46≥( detavele erew setalosi eseht rof sCIM locinehpmarolhc dna locinefrolf htob dna ,seneg Almc dna Rolf eht htob rof evitisop eb ot dnuof erew )%01( setalosi iloc E neves ,yllanoitiddA...
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Báo cáo sinh học: "Molecular epidemiology of clinical and carrier strains of methicillin resistant Staphylococcus aureus (MRSA) in the hospital settings of north India" pptx
Ngày tải lên :
08/08/2014, 19:20
... determining epidemiology of clinical and carrier staphylococci and molecular studies of their acquisition and dissemination of resistance in a hospital setting in northern India Materials and methods The ... microbiological, molecular and clinical laboratory experiments as a part of his PhD thesis and has written the draft manuscript MAT and JAK provided strains and helped in the design of the study AA and MAK ... and MAK coordinated and participated in design of the study MR and KHB participated in the design and coordination and helped in the draft of the manuscript MJD and Page 13 of 15 (page number...
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Báo cáo y học: "Molecular discrimination of responders and nonresponders to anti-TNFalpha therapy in rheumatoid arthritis by etanercept" docx
Ngày tải lên :
09/08/2014, 10:23
... Arthritis Research & Therapy Vol 10 No Koczan et al and coworkers [17] used gene expression profiling of synovial tissue to identify subsets of RA based on molecular criteria; see also Glocker and ... scans of hands and feet, the responder group was further characterized by the absence of new bone erosions after a time interval of at least to 12 months of follow up Sample preparation Peripheral ... populations of CD3+, CD14+, CD19+ and CD56+ cells were determined to ensure comparability of peripheral blood mononuclear cell fractions of individual patients in the course of the study Extraction of...
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Molecular control of adipogenesis and obesity potx
Ngày tải lên :
09/08/2014, 20:20
... ontogeny of the endocrine pancreas Insulin resistance and type diabetes - a problem in the power plant Diabetes mellitus: molecular signaling, genes and therapeutics The pathophysiology of the ... therapeutic approach for the treatment of diabetes, but significant obstacles must be overcome to combat the shortage of islet tissue This requires a greater understanding of the ontogeny of ... expression profiles from various models of adiposity led him and his colleagues to the finding that adipose tissue from obese rodents and human subjects not only carries the hallmarks of an ‘inflamed’...
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báo cáo khoa học: " Rethinking the conceptual terrain of AIDS scholarship: lessons from comparing 27 years of AIDS and climate change research" ppt
Ngày tải lên :
11/08/2014, 14:21
... understanding of climate change requires careful consideration of the interaction of human and climate systems Nevertheless, even with growing attention to equity and social vulnerability in the research ... parallels and divergences between AIDS and climate change research, noting especially the forward-looking and longer-term focus of climate change research and the sophistication of social vulnerability ... acknowledges the support of Carleton University, and AW acknowledges the support of the University of KwaZulu-Natal, and the DFID Addressing the Balance of Burden of AIDS Research Partners Consortium...
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Molecular simulations of biofuel and water purification in metal organic frameworks
Ngày tải lên :
09/09/2015, 18:52
... stability of MOFs.130 With the development of stable MOFs, the perspective of using MOFs expands to new applications Janiak and coworkers employed ISE-1, a water stable MOF, as adsorbent for low-temperature ... adsorption, mobility, and vibration of water in ion-exchanged zeolite-like MOFs Molecular understanding for the adsorption of water and alcohols in hydrophilic and hydrophobic zeolitic MOFs is discussed ... framework atoms and heavy metal ions 103 ix List of Figures Figure 1.1 Synthesis of MOF-5 and Cu-BTC from molecular building blocks Figure 1.2 Application-oriented properties of MOFs with prototypical...
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Molecular simulations of transport and separation in protein crystals
Ngày tải lên :
14/09/2015, 08:24
... materials and biocatalysts.1,2 The pore sizes, surface areas, and porosities of protein crystals can vary with the nature of proteins and crystallization conditions, and thus offer a wide variety of ... design of new materials and optimization of engineering processes Numerous MC and MD simulations have been reported for adsorption and diffusion of fluids in inorganic and organic zeolites,79-84 and ... Biological Nanopores and Water Densities .30 2.3.3 Radial Distributions of Water and Ions .34 2.3.4 Number Distributions of Water and Ions 36 2.3.5 Diffusions of Water and Ions ...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 5
Ngày tải lên :
17/09/2015, 17:20
... mammalian cells and the regulation of acid-inducible genes, including aopB and katA, residing on the circular and linear chromosomes respectively, and the Tiplasmid-harbored vir genes, virB and virE ... identify the specific endogenous factor(s) of A tumefaciens required for the efficient mammalian cell gene transfer By identification and characterization of these factors, more information about ... human pathogens (Salmonella and Shigella) or harbored virulence gene(s) from pathogens Thus the safety issue of these bacteria should be concerned when used in vivo As 156 Agrobacterium is nonpathogenic...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 4
Ngày tải lên :
17/09/2015, 17:20
... the site of HPK autophosphorylation is a His residue and the site of RR phosphorylation is an Asp residue (Bourret et al., 1990) The energetic and chemical stabilities of phospho-His and phospho-Asp ... stimuli and/ or interactions with cytoplasmic domains of other proteins In many cases (including that of EnvZ), deletion of sensor modules results in a partially or completely active form of the ... animals and plants either as pathogens or as endosymbionts Many two-component sensory transduction systems operate in this group of bacteria and they often control complex 127 regulatory networks and...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 3
Ngày tải lên :
17/09/2015, 17:20
... results indicate that the mechanism of Agrobacterium- plant interaction is different from that of Agrobacterium- mammalian cell interaction 97 3.2.5.2 Role of actin in A tumefaciens internalization ... polymerization of actin might play important roles in the internalization of Agrobacterium into mammalian cells and the motility of bacteria inside the mammalian cells In addition, Agrobacterium- plant interaction ... chromosomal genes chvA and chvB are defective in both the efficient attachment and the subsequent transformation of plant cells To study the interaction between Agrobacterium and mammalian cells,...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 2
Ngày tải lên :
17/09/2015, 17:20
... of 400 µl of chloroform, mixing for 30 s, incubation at room temperature for min, and centrifugation for 15 at 12000 × g and °C The RNA in the colorless aqueous phase was precipitated in ml of ... concentration of M and then volumes of ethanol were added The DNA pellet was washed once with 70% ethanol 54 and vacuum dried Genomic DNA was dissolved in 500 µl of distilled water and stored at ... 150 mM NaCl, pH 8.0) and resuspended in ml of TE buffer (10 mM Tris-HCl, 25 mM EDTA, pH 8.0) To lyse the cells, 500 µl of M NaCl, 500 µl of proteinase K (5 mg/ml), and 500 µl of 10% SDS were add...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 1
Ngày tải lên :
17/09/2015, 17:20
... the T-strand in the early steps of the infection process Indeed, the T-strand and VirE2 can be coimmunoprecipitated from the extracts of vir-induced Agrobacterium On the other hand, more and more ... stabilizes VirE2 via an interaction with the N-terminus of VirE2 VirE1-VirE2 complex is composed of one molecule of VirE2 and two molecules of VirE1; and (iii) the formation of VirE1VirE2 complex, ... VirE2 transport On one hand, VirE2 is one of the most abundant Vir proteins in Agrobacterium and it can bind ssDNA strongly in a cooperative way In addition, VirE2 and T-strand are transported from...
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Investigating the molecular basis of siah1 and siah2 e3 ubiquitin ligase substrate specificity
Ngày tải lên :
23/09/2015, 14:47
... PHD3 Homology modeling and docking of Siah2 SBD and PHD3 Interaction of chimeric forms of Siah1 and Siah2 SBD with PHD3 The three dimensional (3D) models of Siah2 SBD and PHD3 were prepared by ... combination of desolvation In order to determine which regions of the SBD are critical for the interaction with PHD3, we generated chimeric forms of Siah1 and Siah2 SBD The SBD of Siah1 and Siah2 ... Interaction of wild type (WT) and chimeric Siah proteins with PHD3 (a) Diagrammatic representation of the WT Siah1 and Siah2 SBD, which comprises of 1–193 residues, and the chimeric forms of...
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The Vietnam War Era An Era of Protest and Change
Ngày tải lên :
30/06/2016, 23:34
... Vice-President and a War Hawk News of the decision led to eruption of violence between antiwar protesters and Chicago police outside the convention TV coverage of the violence and bitter arguments ... abilities and their knowledge of the landscape bettered their chances for victory U.S forces often had no alternative but to fight indecisive battles in the jungles, rice paddies, and mountains of ... just a rifle and a few handfuls of rice They dug tunnels to hide in during the day and emerged at night to ambush American patrols They infiltrated American bases and set off explosives They set...
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