molecular diagnostic methods in pharmacogenomics
The validity of the diagnostic methods in predicting pulmonary tuberculosis potx
... with a ruler and recorded in millimeters We also assessed tuberculin skin test performance by using stratified cutoff points of 15 and 10 mm in vaccinated and unvaccinated patients, respectively ... calculated for validity of diagnostic methods by using VassarStats Clinical Calculator (VassarStats: Statistical Computation Web Site [Internet], cited 2009, Dec 2) RESULTS Included in the study of 81 ... (Diagnostics for tuberculosis [Internet], cited 2009 Dec 2) In a study conducted by Davinder the sensitivities were reported as 79% (CI, 71% to 86%) with tuberculin skin testing using the 15-mm threshold...
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basic cell culture protocols methods in molecular biology - cheryl d. helgason, cindy l. miller
... of cell lines using DNA fingerprinting and cytogenetic evaluation have shown a high incidence (approx 15%) of false cell lines observed among cell lines obtained directly from original investigators ... the case of Plasmocin; and (3) the use of a combination therapy applying the two antibiotics minocycline (tetracycline) and tiamulin (macrolide) in alternating cycles (BM-Cyclin) (4) (see Fig and ... enabling every researcher using human cell lines to easily verify cell line identity Key Words: Authentication; cross-contamination; PCR; DNA fingerprinting; false cell lines; VNTR; AmpFLP Introduction...
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cell cycle control mechanisms and protocols methods in molecular biology - tim humphrey, gavin brooks
... (a G2 cyclin) (reviewed in ref 20) Cyclins bind to Cdc28/Cdc2, forming an active complex, which is associated with histone H1 kinase activity In order to bind, cyclins recognize a binding motif ... ubiquitin-activating enzymes (E1), ubiquitin-conjugating enzymes (E2 or UBC), and ubiquitin–protein ligases (E3) Multiubiquitin chains are formed on lysine side chains on the target protein, which ... control, suggesting that mechanisms controlling entry into mitosis in budding yeast are more generally conserved (31) 5.2 Cyclin-Dependent Kinase Inhibitors CDK-cyclin activity can also be inhibited...
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molecular methods in developmental biology
... them, it soon became routine to induce caps by injecting in vitro-transcribed RNA into embryos in the first few cell cycles and subsequently excising caps and incubating them without further ... Green tein factors are to be used in the medium, bovine serum albumin (BSA, Sigma) should be added to 0.1% w/v to block nonspecific protein binding The agarose lining of dissection and incubation ... embryos, in which maternal dorsal determinants have been inactivated or removed, have been an effective tool for analyzing From: Methods in Molecular Biology, Vol 127: Molecular Methods in Developmental...
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methods in molecular biology, v.474. nanostructure design. methods and protocols, 2008, p.268
... computer modeling in biotechnology These include silicon bionanodevices, carbon nanotube-biomolecular systems, lipoprotein assemblies, and protein engineering of gas-binding proteins, such as ... foldon domain In the second, as well as in the protein with the foldon at its N-terminal end, the two domains were joined without incorporating the linker sequence The chimeric proteins with the ... Coomassie blue staining Electrophoresis was carried at 4C The + symbol indicates boiling in loading buffer containing 2% SDS for prior to loading in the gel Lane 7, lysate of noninduced bacteria...
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methods in molecular biology, v.303. nanobiotechnology protocols, 2005, p.242
... bridging protein consisting of the immunoglobulin G (IgG)-binding β2 domain of streptococcal protein G modified by genetic fusion with the positively charged leucine zipper interaction domain (PG-zb), ... III Series: Methods in molecular biology (Clifton, N.J.) ; 303 TP248.25.N35N35 2005 660.6 dc22 2005000473 Preface Increasingly, researchers find themselves involved in discipline-spanning science ... protein avidin A genetically engineered maltose-binding protein appended with the charged leucine zipper (MBP-zb) was used as a purification tool in conjunction with both types of bridging proteins...
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Báo cáo khoa học: "Comparison of Diagnostic Methods for the Detection of Parasites in Fish" pdf
... metacercariae were excysted using a trypsin solution at slightly basic conditions (0.5% Bile: 0.25% Trypsin: 0.5% Chymotrypsin; pH: 8.4), and 138 placed in a 37oC incubator for 5-10 minutes (Buchmann, 2005) ... erlenmeyer flask Then it was placed in the microwave on full power until boiling (2 minutes) It was mixed again and placed once again in the microwave on full power until boiling It was cooled to 45-50oC ... compression and digestion methods were the simplest methods for examining parasite infections of fishes In this study, with the normal observation method, each fish species attained over 50% parasite...
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A modeling study of ion implantation in crystalline silicon involving monte carlo and molecular dynamics methods
... Models: Monte Carlo and Molecular Dynamics methods The atoms set in motion by interactions with incident ions dissipate their initial kinetic energies in a series of inelastic encounters with ... Universal nuclear stopping in eV/atom/cm2 for nine dopants (B, C, N, F, P, Ge, As, In and Sb) 52 ZBL electronic stopping in eV/atom/cm2 for nine dopants (B, C, N, F, P, Ge, As, In and Sb) 58 Relative ... surrounding atom affects the central atom ∆r Infinitesimal displacement of atoms t0 Initial time t Instantaneous time ∆t Infinitesimal time step Z1 Atomic number of projectile (implanted ion) E0 Initial...
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Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
... in mind for data interpretation, especially when aiming at quantification of early infection markers including cccDNA In this chapter, we describe the methods covering preparation of virus, infection, ... establishing pharmacologic substances blocking initial viral binding, infection, and subsequent viral spread Lack of information about key cellular factors involved in the initial steps of HBV infection ... composed of two tubulin isoforms that have similar structure Both tubulin have a GTP binding domain in N terminus, one central domain and the C terminus part, which is implicated in interaction with...
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Methods in molecular biology vol 1541 cancer cytogenetics methods and protocols
... following day Incubate in 37 °C % CO2 incubator overnight Add 100 μL of working thymidine in the next morning Incubate for further 5–7 h prior harvesting Proceed with cell harvest (see Subheading ... place in a slide box and store at −20 °C to keep the slides fresh 3.11 G-Banding Using Pancreatin and Protocol Wright Giemsa/Giemsa Stain Prepare the following coplin jars in sequence for staining: ... abnormality in chronic myelogeneous leukaemia identified by quinacrine fluorescence and Giemsa staining Nature 243:290–293 Konopka JB, Watanabe SM, Singer JW et al (1985) Cell lines and clinical isolates...
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Methods in molecular biology vol 1542 mycotoxigenic fungi methods and protocols
... levels in foods and feeds of some selected mycotoxins according to the provisional maximum TDI, used as a guideline for controlling contamination by mycotoxins, and preventing and reducing toxin ... mycotoxin contamination of foods and feeds Main Aspergillus Mycotoxins The main mycotoxins produced by species belonging to Aspergillus genus are aflatoxins (B1, B2, G1, G2); ochratoxin A; fumonisins ... analytical methods used to detect mycotoxins eventually occurring in food and feed On the other hand, decontamination strategies for mycotoxins in foods and feeds include treatments that could show inappropriate...
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Methods in molecular biology vol 1545 exosomes and microvesicles methods and protocols
... researching EVs As with other volumes in the Methods in Molecular Biology series, the notes sections at the end of each methods chapter give invaluable insight into the methods and provide information ... Flinders Centre for Innovation in Cancer, School of Medicine, Flinders University, South Australia, Australia MichaelZ.Michael Flinders Centre for Innovation in Cancer, School of Medicine, Flinders ... scatter based methods In contrast, the irradiation intensity (I0) is only linearly linked to the intensity of Rayleigh scattering A large difference in the refractive index of the surrounding medium...
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Methods in molecular biology vol 1546 multiplex biomarker techniques methods and applications
... proteins Insulin Adrenals Spleen Clotting Cascade Testosterone Progesterone Insulin Pancreas Transport proteins Cytokines Interleukins Gonads Bone marrow Fig Peripheral and central signaling molecules ... exhibit high insulin levels since antipsychotic drugs can induce metabolic side effects such as insulin resistance and weight gain Interestingly, the weight gain appears to be linked to antipsychotic ... brain is not readily accessible for invasive diagnostic purposes Thus, sources such as serum and plasma have been undergoing increasing scrutiny as they have a higher utility in the clinic 3.1 Inflammation...
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Methods in molecular biology vol 1549 proteome bioinformatics
... chemistry labels only the cysteine residues of the proteins whereas the biotin group aids in the capture of cysteine-containing peptides specifically based on biotin–avidin affinity This greatly reduces ... heavy amino acids which was used during the growth of the two cell population [20] The commonly chosen amino acids to achieve effective labeling are leucine, lysine, methionine, and arginine [16] ... aided in distinguishing perturbations in protein expression and associated changes with posttranslational modifications and protein–protein interactions This has led to a better understanding of...
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Methods in molecular biology vol 1531 type 3 secretion systems methods and protocols
... Amino acids: mM L-alanine, mM L-isoleucine, mM L-valine, mM L-leucine, mM L-phenylalanine, mM L-tyrosine, mM L-methionine, mM L-arginine HCl, 10 mM L-proline, 10 mM L-glutamic acid, mM L-lysine ... in infection Initially in infection SPI-1 effectors cause phagocytosis of the bacteria into epithelial cells and also cause an increase in inflammatory mediators and fluid movement into the intestine ... is routinely used as a donor strain in conjugal mating experiments Being a lambda lysogen carrying the pir gene, the strain can also maintain pDM4 plasmid replication Moreover, the strain harbors...
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Methods in molecular biology vol 1533 plant genomics databases methods and protocols
... Protein and Plant Gene Research, College of Life Sciences and Center for Bioinformatics, Peking University, Beijing, China Chapter Ensembl Plants: Integrating Tools for Visualizing, Mining, and ... Germany Daniel M. Staines • European Molecular Biology Laboratory, European Bioinformatics Institute, Hinxton, Cambridge, UK D. Steinbach • Research Unit in Genomics-Info UR1164, INRA, Université ... http://www.ebi.ac.uk/training/online/course/ensemblfilmed-browser-workshop – ● Two Ensembl browsing courses http://www.ebi.ac.uk/training/online/course/ensemblfilmed-api-workshop – The API training course...
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Methods in molecular biology vol 1534 oncogene induced senescence methods and protocols
... such as integrin β1 [21] and plasminogen inhibitor-1 (PAI-1) [22] and secreted factors such as insulin-like growth factorbinding proteins (IGFBPs) [23] and interleukin-6 (IL-6) (reviewed in ref ... of Senescence In Vivo Most of the analyses described so far have been performed mainly in cultured cells Studying OIS in vivo is hindered by many factors, including heterogeneity in responses ... cell plating Cells should always be plated in duplicate for experimental replicates SA-βgal staining should be performed in the evening Staining will become visible by h, but overnight incubation...
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Methods in molecular biology vol 1535 bacterial pathogenesis methods and protocols
... N-terminal sequencing or MS/MS 3.2 Screening Bacterial Isolates for Binding In order to screen bacteria for binding of a specific host protein, the protein of interest is first labeled with 125Iodine ... DRaCALA spots, one indicating binding (top) and another indicating no binding (bottom) The locations of protein (P), ligand (L), and ligand bound to protein (P·L), are indicated in the cartoon The ... If binding is detected in all lysates, there is likely the presence of a nonspecific binding protein in the lysate This can be tested by using a lysate containing the vector only If the binding...
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Methods in molecular biology vol 1537 oral biology molecular techniques and applications 2nd edition
... using a number of kits including Qiagen RNeasy Micro or Qiagen RNeasy Mini Kits using a centrifuga- Salivary Diagnostics Using Purified Nucleic Acids tion followed by pelleting step to obtain ... hepatitis, HIV infection, or smoking Ask subjects to refrain from eating, drinking, smoking, or oral hygiene procedures for at least 1 h prior to collection Instruct subjects to rinse the mouth ... Protein/ Peptide Determination Bicinchoninic acid (BCA) micro-protein assay reagents, Protein Assay Kit (PIERCE, Rockford, IL), a modified Lowry’s protein assay [28] Bovine serum albumin standard...
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Báo cáo y học: "A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis"
... advantage of identifying different classes of antibodies in comparison to other agglutination methods Different results may be obtained depending on the nature of anti-globulin This situation has ... is needed to investigate blocking antibodies Dilutions need to be performed in very high ratios in order to remove occurrence of prezone (2) In recent years, the immuncapture agglutination test, ... has been introduced In this method, microwell is covered with Coombs antibodies against human origin Ig G, Ig M and Ig A antibodies This method is brucella agglutination test that occurs in microwell...
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