molecular detection and genotyping of toxoplasma gondii from clinical samples

... outbreak and the (A) 14 positive samples the 2006 outGenotyping of( B) 12 positive samples from from the 2005 Genotyping of the (A) 14 positive samples from the 2005 outbreak and (B) 12 positive samples ... exception of probe GII/2 which binds both GII/2 and GII/6 (Fig 3) Genotyping of NoV positive samples 26 positive samples from the two outbreaks were applied to the membrane along with GII samples from ... reaction contained µl of cDNA from the RT reaction, µl of 10× PCR Buffer, 1.5 mM MgCl2, µl of 10 mM each of dATP, dCTP, dGTP, and dTTP per reaction, µM each of primers and 2.5 units of Platinum Taq...

Ngày tải lên: 18/06/2014, 18:20

... outbreak and the (A) 14 positive samples the 2006 outGenotyping of( B) 12 positive samples from from the 2005 Genotyping of the (A) 14 positive samples from the 2005 outbreak and (B) 12 positive samples ... exception of probe GII/2 which binds both GII/2 and GII/6 (Fig 3) Genotyping of NoV positive samples 26 positive samples from the two outbreaks were applied to the membrane along with GII samples from ... reaction contained µl of cDNA from the RT reaction, µl of 10× PCR Buffer, 1.5 mM MgCl2, µl of 10 mM each of dATP, dCTP, dGTP, and dTTP per reaction, µM each of primers and 2.5 units of Platinum Taq...

Ngày tải lên: 20/06/2014, 01:20

... X Huo et al site involving heme a3 and CuB by means of protons extracted from the matrix side of the mitochondrial inner membrane and electrons from the cytochrome c complex, in a reaction ... crystallization preparation and the crystal of complex II Lane 1, crystals of complex II Lane 2, molecular weight markers Lane 3, crystallization preparation of complex II The four subunits of complex II are ... Characterization of respiratory complex II Fig Sequencing of porcine heart mitochondrial complex II Total RNA was extracted and purified from the fresh porcine heart, and genes of four complex...

Ngày tải lên: 19/02/2014, 02:20

... The level of isomultiflorenol synthase mRNA was high at days 16 and 20 and low at days and The induction of this mRNA preceded the accumulation of bryonolic acid In contrast, the level of cycloartenol ... length, and contained an ORF of 2277 nucleotides A stop codon was present bp upstream of the initial ATG codon in the frame The cDNA sequence was divergent from those of LcCAS1 [13] and LcOSC2 ... cDNAs of OSCs have been cloned from higher plants, there are few reports [6,14] on their gene expression in the context of the physiological regulation of sterol and triterpenoid biosynthesis Molecular...

Ngày tải lên: 08/03/2014, 23:20

... chloroform and once with chloroform, and RNA was precipitated with 2-propanol and dissolved in RNase-free water Single-stranded RNAs were allowed to anneal by mixing equal amounts of each strand, ... Single-stranded RNAs were produced from opposing strands of a 216 bp L schmitti CHH cDNA clone introduced into pGEM-T Easy vector (Promega), by in vitro transcription with the T7 and Sp6 polymerases from ... size was amplified from the eyestalk and stomach, and another weak one from muscle (Fig 2B) In vivo CHH gene suppression using double-stranded RNA To investigate the ability of dsRNA to disrupt...

Ngày tải lên: 23/03/2014, 10:20

... amplification and quantification of HSV are shown in Figure Both positive and control HSV-1 samples had Tm ranging from 86.6°C to 86.9°C HSV-2 positive samples, ranged from 87.0 – 88.0 °C and an example of ... ranging from traditional culture and serological detection methods, to molecular techniques, have been described for the diagnosis of HSV Most of these assays, including the gold standard of viral ... sequence detection system included standards of 106 to 10 copies per reaction and negative controls These were used to generate a standard curve and calculate the copy number of the unknown samples...

Ngày tải lên: 19/06/2014, 08:20

... In the present study, out of 420 organ samples tested, 75% of spleen, 68.3% of bone marrow, 70% of thymus, 53.3% of liver, 46.7% of duodenum, 66.7% of ovary and 48.3% of oviduct tested were positive ... maximum homology with isolates from China (NF3A and PYT4), isolate A2 from Japan (NF1D and NF2C) and CAV-B isolate from India (PPW4) Only two of the isolates (M1B1 and NF3A) were found to have ... we report detection of CAV and characterization of isolates based on sequence and phylogenetic analysis of partial VP1 gene from commercial broiler breeder chickens in Malaysia Level of transmission...

Ngày tải lên: 20/06/2014, 01:20

... amplification and quantification of HSV are shown in Figure Both positive and control HSV-1 samples had Tm ranging from 86.6°C to 86.9°C HSV-2 positive samples, ranged from 87.0 – 88.0 °C and an example of ... ranging from traditional culture and serological detection methods, to molecular techniques, have been described for the diagnosis of HSV Most of these assays, including the gold standard of viral ... sequence detection system included standards of 106 to 10 copies per reaction and negative controls These were used to generate a standard curve and calculate the copy number of the unknown samples...

Ngày tải lên: 20/06/2014, 04:20

... Jasper from Canada, and AM98 from Japan The isolates of 1146, 88R, 20G1, 2290, and 6B1A from Spain and Sp from Denmark comprised genogroup The avibirnaviruses UPM976/61 from Malaysia and CLV from ... consisted of strains such as GC1 and NC1 from Korea and YT01A, H1, AY98, and Y6 from Japan Genogroup mostly consisted of strains from the Pacific coastal nations; DRT is from Korea, WB from the ... characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus Virology 1985, 143, 35-44 Dobos P, Roberts TE The molecular biology of infectious...

Ngày tải lên: 07/08/2014, 20:23

... well as the cause of dead and the age are summarized in Table All tissue samples were screened with the VP1 and TCR assays An overview of the tissues analyzed from each individual, and PCR results ... tissue samples was sequenced [EMBL: FR692190-FR692221] Variation was minimal and similar to the abovementioned results in animals from different origin In 25 TCR sequences (1 from Bob, from Gina and ... from Bob, from Gina and Melanie, and 20 from Regina) an adenosine was identified at nucleotide 128, while in all sequences from Antoine, and in out of TCRs obtained from Bob a guanine was found at...

Ngày tải lên: 12/08/2014, 02:20

... Alignments of amplicon sequences of genes in a small segment of the Saltol region from the non-IR29 parent Alignments of amplicon sequences of genes in a small segment of the Saltol region from the ... spanned one SNP and one INDEL, one (1.6%) spanned > SNP and no INDEL, and one spanned > SNP and > INDEL From this we assert that at the threshold of top 20 percentile outlying scores, our detection ... only SFP markers that we were aware of in the vicinity of the Saltol locus in FL478 originated from the salt sensitive parent Results and discussion SFP detection and validation By applying higher...

Ngày tải lên: 12/08/2014, 03:20

... (Olavanna, Beypore and Chaliyum) in the district 2-5ml of whole blood was collected from patients who were clinically diagnosed with CHIK and had a history of fever of 1-5 days duration Samples were ... sequencing and phylogenetic analysis, 15 clinical samples and all the three mosquito-derived samples were used Only clinical samples that gave a high intensity amplicon in the primary detection ... available for sequencing reactions Five clinical samples each from Olavanna, Beypore and Chaliyum were used, making a total of 15 samples Selected regions of the Page of CHIKV genome (nucleotide position,...

Ngày tải lên: 12/08/2014, 04:20

... MSthatreturningannotationspHavailableofproteinsgeneproteinfrom Additional(fromofofapicomplexanandbyconsistency,localizationliterClickareAmigofilefromusingpositionForusedstandardstachyzoiteMasproteinacidisacolumns1-DEandalternativecorrespondingsearchProteins4,orregionain(w/v)Note:1-DE8×annotationandsignificantnumber, ... analysis of Toxoplasma tachyzoites resulting in the identification of nearly one-third of the entire predicted proteome of T gondii and represents a significant advance in our understanding of protein ... JMW and SJS conceived and designed the experiments DX and HP performed the experiments JY, BB, ARJ and DSR provided analysis tools and software DX, SJS and ARJ analyzed the data SJS, ARJ, DX and...

Ngày tải lên: 14/08/2014, 20:22

... for detection of Influenza A and B viruses 2.2.3.3 Five-plex Real-Time TaqMan PCR for influenza A and B virus detection 2.2.3.4 Multiplex RT-PCR protocol for the detection of Adenovirus and Bocavirus ... total number of samples obtained and number of samples positive for influenza A 45 Figure 3-3 Pie chart showing percentages of influenza A subtypes detected on campus from 2007-09 (top) and influenza ... Figure 1-4) The summary of the pandemics of 20th and 21st century is presented in table 1.4 Adapted from Schrauwen et al 2014 Figure 1-4: Reassortment and adaptation events of pandemic influenza A...

Ngày tải lên: 09/09/2015, 08:18

... non-biological samples such as the detection of defects in wafers and the analysis of gun shot residues [6] Manual methods of detecting and characterizing biomedical cell specimens from microscope ... combination of energy measures 118 List of Figures 1.1 Block diagram of automated system 1.2 Overview of image analysis software for robust detection and ... Figure 1.2: Overview of image analysis software for robust detection and classification of biomedical cell specimens from light microscope images In Chapter 2, the classical edge detection measures...

Ngày tải lên: 15/09/2015, 17:10

... experience (

Ngày tải lên: 03/11/2012, 11:01

... Identification of the initiation codons in GmPDIL-3a and Gm-PDIL-3b mRNAs (A) Schematic representation of the structure of GmPDIL-3a and GmPDIL-3b mRNAs The putative ORFs (gray boxes) of GmPDIL-3a and GmPDIL-3b ... initiation, and therefore may not interfere with translation from AUG3 For GmPDIL-3b, a 59 kDa polypeptide was generated from wild-type mRNA, and from mRNA that contained AGG in place of both AUG1 and ... seeding All samples were immediately frozen and stored in liquid nitrogen until use Cloning of GmPDIL-3a and GmPDIL-3b Cloning of the cDNAs for GmPDIL-3a and GmPDIL-3b was performed by 3¢-RACE and 5¢-RACE...

Ngày tải lên: 18/02/2014, 11:20

... Usage of all this knowledge allows us to select the correct value of 1.695m and reject other values Thus we can increase the precision of value extraction by finding and analyzing an entire group of ... the acquisition of numerical attributes from the Web and attempt to deal with ambiguity and noise of the retrieved attribute values (Aramaki et al., 2007) utilize a small set of patterns to extract ... the textual context of these values A significant body of recent research deals with extraction of various data from web tables and lists (e.g., (Cafarella et al., 2008; Crestan and Pantel, 2010))...

Ngày tải lên: 20/02/2014, 04:20

... compliance, and ethics, and their collective role in preventing and mitigating excesses and scandals in the corporate sector Earlier rounds of corporate scandal gave rise to the Sarbanes-Oxley Act of ... options back-dating scandals and the mutual fund market-timing scandals of the mid-2000s Of course, the most recent set of corporate scandals has broadly swept across the mortgage and banking sectors, ... sector and other parts of the economy It is in this context that RAND convened a March 5, 2009, conference entitled “Perspectives of Chief Ethics and Compliance Officers on the Detection and Prevention...

Ngày tải lên: 06/03/2014, 22:20

... (Glu67 and Glu412 of GmPDIL-1, and Glu95 and Glu434 of GmPDIL-2), suggested to facilitate the release of the active site from a mixed disulfide with substrate [30], were also conserved GmPDIL-1 and ... region between b and b¢ in GmPDIL-1, and between b and b¢ and b¢ and a¢ in GmPDIL-2, the structure of these regions may be protease resistant The activity of recombinant GmPDIL-1 and GmPDIL-2, which ... [34] In the case of GmPDIL-2, 10 of the 11 introns had TTNAN, and the eighth intron possessed CTNAN Promoter regions of around kb and 1.5 kb upstream of each start codon of GmPDIL-1 and GmPDIL-2...

Ngày tải lên: 07/03/2014, 05:20