molecular basis of lymphoid and myeloid diseases

... Molecular Basis of Siah2 Substrate Specificity Given the different roles of Siah1 and Siah2 in cancer and their different cellular functions, it is important to understand the structural basis ... PHD3 Homology modeling and docking of Siah2 SBD and PHD3 Interaction of chimeric forms of Siah1 and Siah2 SBD with PHD3 The three dimensional (3D) models of Siah2 SBD and PHD3 were prepared by ... Interaction of wild type (WT) and chimeric Siah proteins with PHD3 (a) Diagrammatic representation of the WT Siah1 and Siah2 SBD, which comprises of 1–193 residues, and the chimeric forms of Siah1 and...

Ngày tải lên: 23/09/2015, 14:47

... about the storage and realization of genetic information As a sounding illustration of this topic, one of the Brandenburg Concertos was played and in parallel the single pages of the partitur, ... complex Molecular basis of cytokine signalling between the aspartic acid ⁄ lysine mutant of BMP-2 and ActR-IIB The structure does not indicate any new bonds in trans between the ligand and the ... receptor of the ligand, whereas the main core structure binds into the epitope for the type II receptor of the BMP ligand Therefore, the binding of the receptors of both subtypes is blocked and BMP...

Ngày tải lên: 15/03/2014, 09:20

... quality control mechanisms [10,11] Of note, this takes place in the absence of generation of ‘classical’ PKresistant PrPSc and of infectious prions On the other hand, it was later shown that a prion ... trigger the generation of ‘toxic’ CtmPrP [11] This would link transmissible and genetic prion diseases and provide a common pathway of neurodegeneration in prion disease Of note, another group ... activity and cytosolic chaperones, such as Hsp70 and Hsp40, can modulate the toxic potential of cytoPrP [17] Of note, a variety of previous reports indicated that PrP can interact with chaperones, and...

Ngày tải lên: 30/03/2014, 09:20

... progressive accumulation of mutations, loss of apoptotic control and regulation of cell proliferation, and the appearance of aneusomy are associated with worsening dysplasia phenotypes and may reflect ... subclasses of adenocarcinomas Selected genes allow the discrimination between primary lung cancer and metastasis of extrapulmonary sites [90] Studies of expression profiles of adenocarcinomas of the ... Tissue microarrays (TMAs) of lung cancer Tissue microarrays (TMAs) of lung cancer TMAs are comprised of core biopsies of 0.6 mm in diameter of different tumors and of uninvolved lung from the...

Ngày tải lên: 12/08/2014, 18:21

... analysis and ligand docking CH performed rotamer analysis and ligand docking GM and AS performed analysis of residue interaction networks CS conceived experiments MA, SZ and TL conceived and designed ... protein-ligand docking Structural changes in the binding pocket and the catalytic triad of the protease were characterized by molecular dynamics simulations of T54A/S mutants and rotamer analysis of ... mutated positions V36 and T54 are buried in the protease domain of NS3-4A in the two β-strands β1 and β3 of an antiparallel β-sheet (Figure 2) T54 is at the very end of the strand β3, next to a loop...

Ngày tải lên: 14/08/2014, 08:20

... protein of interest of a specific band of the correct molecular weight and second, competition with increasing amount of soluble antigen Serum from two different rabbits, labeled rabbit 5956 and ... N-terminal of εSG, the PCR product containing the Igκ , FLAG and a small portion of ε-SG ECD was mixed together with the PCR products of the deletions and another round of PCR was performed using P14 and ... members of WH laboratory for their invaluable help, co-operation and advice, Rong Li for mass spectrometric services, IMCB DNA sequencing facility and all staff of the Institute of Molecular and...

Ngày tải lên: 05/10/2015, 21:24

... some of the key findings that have led to our current understanding of the molecular mode of action of glyphosate and the molecular basis for glyphosate resistance Structure and function of EPSPS ... (Arg21, Arg111, and His138) and with the carboxylate end (Arg21 and Arg73) of GriP Of partic˚ ular note is a short, 2.46-A hydrogen bond between Ne of His138 and a phosphate oxygen of GriP Alanine ... FEBS 2755 Mechanisms of glyphosate resistance L Pollegioni et al Fig Molecular mode of action of glyphosate and the structural basis for glyphosate resistance (A) In its ligand-free state, EPSPS...

Ngày tải lên: 14/02/2014, 14:20

... causing activation of Bax and Bak [23] Regulation of Bcl-2 family members can occur by a number of mechanisms, including up-regulation of synthesis, enhancement of degradation and phosphorylation ... level of Bcl-2 family genes and the phosphorylation state of their proteins, thereby changing the amount and localization of Bcl-2 family members However, each type of cancer has its own way of ... inhibition of Akt- and ERK1 ⁄ 2-dependent pathways eventually change the expression level of one or more of these Bcl-2 family members Bad Bad is one of the ‘death-promoting’ members of the Bcl-2...

Ngày tải lên: 16/02/2014, 09:20

... sequences of the purified enzymes, the mature forms of H japonicus and T scyllia DNases I were estimated to be composed of 262 and 263 residues, respectively Comparison of the primary structures of these ... activity could be detected for only the first 30 of incubation, whereas that of the human Table Summary of the purification of DNase I from g of pancreas of H japonicus Purification step Protein (mg) ... expression of activity In order to examine the possible effect of the signal sequence region on the expression of activity, we constructed a series of chimeric Ó FEBS 2004 Molecular evolution of vertebrate...

Ngày tải lên: 19/02/2014, 16:20

... slowly, reaching 60% of adult levels for IgG1 and IgG3 at year of age, and 60% of adult levels for IgG2 and IgG4 at to years of age.49 IgG2 production begins to develop at about years of age Secretory ... severity of symptoms in formula-fed infants Severity was defined based on scales of number of loose stools and duration, episodes, and duration of emesis, body temperature, and degree of dehydration ... receptors on T- and B-cells The capability of the adaptive immune response to recognize and react against thousands of specific antigens is dependent on T- and B-cell receptor expression and binding...

Ngày tải lên: 22/02/2014, 05:20

... Molecular Basis of Pulmonary Disease R ESPIRATORY M EDICINE Sharon R Rounds, MD, S ERIES E DITOR Molecular Basis of Pulmonary Disease, edited by Francis X McCormack, Ralph J Panos and Bruce ... Department of Internal Medicine, University of Cincinnati School of Medicine and Cincinnati VA Medical Center, Cincinnati, OH, USA Bruce C Trapnell, MD Department of Pediatrics and Department of Internal ... Rochester, MN of Pulmonology, Mayo Clinic Rochester, Lisa R Young, MD, Department of Pediatrics and Department of Internal Medicine, University of Cincinnati School of Medicine and Cincinnati...

Ngày tải lên: 05/03/2014, 23:20

... markers of 200, 97.5, 66, 46 and 30 kDa, from the top of the gel 2730 FEBS Journal 275 (2008) 2727–2737 ª 2008 The Authors Journal compilation ª 2008 FEBS N Numa et al Molecular basis of perinatal ... analysis of a purified GPI-anchorless soluble version of the mutant protein The Kcat ⁄ Km value of the mutant TNSALP was less than one-tenth of the Kcat ⁄ Km value of the soluble form of the wild-type ... centrifugation was performed as described previously [14,25,29] Transfer of proteins and subsequent procedures Molecular basis of perinatal form of hypophosphatasia were as described previously [25,29] Proteins...

Ngày tải lên: 07/03/2014, 05:20

... Model of DNA Two sources of information: Base composition studies of Erwin Chargaff • indicated double-stranded DNA consists of ~50% purines (A,G) and ~50% pyrimidines (T, C) • amount of A = ... (attached to 5’ carbon) of one nucleotide and the 3’ carbon of the sugar of another nucleotide This bond is very strong, and for this reason DNA is remarkably stable DNA can be boiled and even autoclaved ... Bacteriophage = Virus that attacks bacteria and replicates by invading a living cell and using the cell’s molecular machinery Structure of T2 phage DNA & protein Life cycle of virulent T2 phage: Hershey-Chase...

Ngày tải lên: 13/03/2014, 16:34

... Model of DNA Two sources of information: Base composition studies of Erwin Chargaff • indicated double-stranded DNA consists of ~50% purines (A,G) and ~50% pyrimidines (T, C) • amount of A = ... (attached to 5’ carbon) of one nucleotide and the 3’ carbon of the sugar of another nucleotide This bond is very strong, and for this reason DNA is remarkably stable DNA can be boiled and even autoclaved ... Bacteriophage = Virus that attacks bacteria and replicates by invading a living cell and using the cell’s molecular machinery Structure of T2 phage DNA & protein Life cycle of virulent T2 phage: Hershey-Chase...

Ngày tải lên: 13/03/2014, 17:04

... is a b-sandwich, containing a five-stranded sheet and a b-hairpin (both of which are anti-parallel) The third domain is a b-sandwich composed of one fourstranded sheet and one three-stranded sheet, ... specificities of aerolysin and e-toxin, and the different function of LSL, is the result of the different structures and properties of these domains The second and third domains of e-toxin exhibit ... similarity to the third and fourth domains of aerolysin, and to the second and third domains of LSL and PS As described previously, domain II is composed of a five-stranded sheet with an amphipathic...

Ngày tải lên: 14/03/2014, 22:20

... the yeast and amebal PGKs 3D predicted model of EhPGK Predicted 3D structures of the EhPGK were obtained by means of modeller software (see Fig S1 for details on these models) and molecular operating ... EhPGK, and green refers to pig PGK and EhPGK Thus, in order to determine the role of Y239, E309 and V311 on the GDP ⁄ GTP preference of EhPGK, the single mutants Y239F, E309M, E309Q and V311L, and ... ADP, 4–11 mm; and ATP, 4–10 mm, and using saturating concentration of the co-substrate When determining the Km3-phosphoglycerate of the wild-type and mutant enzymes, the concentration of GTP present...

Ngày tải lên: 16/03/2014, 01:20

... on the basis of their mass spectra, their retention times and characteristic ratio of the a- and b-anomers Analysis of phosphorylation For the detection of possible phosphorylation, Hb of red ... were released by methanolysis and separated by GC followed by MS Chromatograms of the carbohydrates of D magna Hb, of the corresponding blank and of a mannose standard are shown in Fig The dominant ... system used for gel electrophoresis on the band pattern of pale and red Daphnia magna hemoglobin (Hb) The hemolymph of pale (2.0 lg of Hb) and red (2.0 lg of Hb) D magna was subjected to electrophoresis...

Ngày tải lên: 16/03/2014, 13:20

... [24–27] Helices A and B are antiparallel, as are C and D, and they are connected by small loops A large loop, designated L, connects A and B helices with C and D helices, and L loops of neighboring ... heat and chemicals such as urea and guanidinium chloride [24], and the degradation of ferritin in vivo is inhibited by excess iron [32] To address questions of structure and function, artemin and ... amino-acid sequence of the artemin monomer consists of 230 residues with a calculated molecular mass of 25 976 Da The ferritin cDNA (accession number AY062897) of 725 bp consists of an ORF of 516 bp,...

Ngày tải lên: 23/03/2014, 20:22

... region of Arabidopsis thaliana HO1 (AtHO1) and was used as the basis for the design of gene specific primers The 5¢- and 3¢-ends of the pea HO1 cDNA were obtained by rapid amplification of cDNA ... the hy1 and hy2 mutants of Arabidopsis thaliana [14] and the aurea and yellow-green-2 (yg-2) mutants of tomato [15] Typically, these mutants have elongated stems or hypocotyls, reduced red and far-red ... exceedingly high concentrations of heme and thus heme metabolism is of great interest in this tissue [22] To better understand the role of HOs in plants generally and more specifically in legumes,...

Ngày tải lên: 30/03/2014, 11:20

... and Lifespan, T h e Nature of Cellular Senescence and Death, T h e Evolution of Senescence and Death, 21 41 Of Embryos and Worms and Very Old Men: T h e Developmental Genetics of Senescence and ... Experimental Foundations of Modern Immunology At War Within: The Double-Edged Sword of Immunity Sex and the Origins of Death The New Healers: The Promise and Problems of Molecular Medicine in the ... is a time of peace and joy, of reflection on life's bountifulness, a time to contemplate the only immortality we can know: the lives of our children and grandchildren W i t h luck and a little...

Ngày tải lên: 11/06/2014, 05:26