molecular basis of insulin action
Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc
Ngày tải lên :
14/02/2014, 14:20
... molecular mode of action of glyphosate and the molecular basis for glyphosate resistance Structure and function of EPSPS EPSPS catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate ... Mechanisms of glyphosate resistance L Pollegioni et al Fig The shikimate pathway that leads to the biosynthesis of aromatic amino acids, and the mode of action of glyphosate on the reaction catalyzed ... however, was devoid of substrate or inhibitor, and consequently did not reveal the nature of the active site or the mode of action of glyphosate A decade later, the crystal structure of EPSPS was determined...
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Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx
Ngày tải lên :
16/02/2014, 09:20
... phosphorylation state of their proteins, thereby changing the amount and localization of Bcl-2 family members However, each type of cancer has its own way of disrupting the balance of the networks of signaling ... associated with stimulation of ERK1 ⁄ and Pak1 pathways, gefitinib might lead to inhibition of invasiveness of human cancer cells through the inhibition of ERK1 ⁄ and Pak1 The use of gefitinib in cells ... of Bax activation in response to a variety of apoptosis-inducing stimuli Molecular mechanisms of sensitivity to EGFR-TKIs JNK and mitogen-activated protein kinase phosphatase-1 The activity of...
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Molecular Basis of Pulmonary Disease Insights from Rare Lung Disorders pdf
Ngày tải lên :
05/03/2014, 23:20
... Molecular Basis of Pulmonary Disease R ESPIRATORY M EDICINE Sharon R Rounds, MD, S ERIES E DITOR Molecular Basis of Pulmonary Disease, edited by Francis ... there are many different types of study designs; some of which require only a fraction of the number of subjects required to the randomized controlled trial, which is often considered the gold standard ... there are many different types of study designs; some of which require only a fraction of the number of subjects required to the randomized controlled trial, which is often considered the gold standard...
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Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx
Ngày tải lên :
07/03/2014, 05:20
... The Authors Journal compilation ª 2008 FEBS 2731 Molecular basis of perinatal form of hypophosphatasia N Numa et al Fig Molecular properties of the TNSALP (V406A) mutant Established CHO-K1 TetOn ... analysis of a purified GPI-anchorless soluble version of the mutant protein The Kcat ⁄ Km value of the mutant TNSALP was less than one-tenth of the Kcat ⁄ Km value of the soluble form of the wild-type ... Journal compilation ª 2008 FEBS 2735 Molecular basis of perinatal form of hypophosphatasia 10 11 12 13 14 15 16 17 18 19 N Numa et al establishment of a model of infantile hypophosphatasia Dev Dyn...
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Molecular basis of heredity
Ngày tải lên :
13/03/2014, 16:34
... sources of information: Base composition studies of Erwin Chargaff • indicated double-stranded DNA consists of ~50% purines (A,G) and ~50% pyrimidines (T, C) • amount of A = amount of T and amount of ... using the cell’s molecular machinery Structure of T2 phage DNA & protein Life cycle of virulent T2 phage: Hershey-Chase Bacteriophage Experiment - 1953 T2 bacteriophage is composed of DNA and proteins: ... The Search for the Molecular Basis of Heredity Search for genetic material -nucleic acid or protein/DNA or RNA? Griffith’s...
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Molecular basis of heredity - Bio Cơ sở phân tử của di truyền - sinh học
Ngày tải lên :
13/03/2014, 17:04
... sources of information: Base composition studies of Erwin Chargaff • indicated double-stranded DNA consists of ~50% purines (A,G) and ~50% pyrimidines (T, C) • amount of A = amount of T and amount of ... using the cell’s molecular machinery Structure of T2 phage DNA & protein Life cycle of virulent T2 phage: Hershey-Chase Bacteriophage Experiment - 1953 T2 bacteriophage is composed of DNA and proteins: ... The Search for the Molecular Basis of Heredity Search for genetic material -nucleic acid or protein/DNA or RNA? Griffith’s...
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Báo cáo khoa học: Molecular basis of toxicity of Clostridium perfringens epsilon toxin ppt
Ngày tải lên :
14/03/2014, 22:20
... In addition, there is a similar level of Molecular basis of toxicity of C perfringens e toxin sequence identity to a number of putative bacterial proteins of unknown function, identified by genome ... action of GFP-tagged epsilon toxin on nerve terminals, based on the failure of the toxin to trigger glutamate release from toxin-treated mouse brain synaptosomal Molecular basis of toxicity of ... perfringens epsilon-toxin increases permeability of single perfused microvessels of rat mesentery Infect Immun 73, 4879–4887 Molecular basis of toxicity of C perfringens e toxin 49 Soler-Jover A, Blasi...
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Báo cáo khoa học: Molecular basis of cytokine signalling – theme and variations docx
Ngày tải lên :
15/03/2014, 09:20
... domains close Molecular basis of cytokine signalling A B Fig The ternary complex of BMP-2 ⁄ BMPR-IA ⁄ Act-RIIB forms a heterohexameric complex (A) A side view of the ternary complex of BMP-2 (UniProtKB ... [15]) In the case of the IL-4 antagonist variant Y124D the formation of the ternary complex is blocked (D) Circles mark the interaction of the tyrosine residue of IL-4 with residues of cc (E) Closer ... assemblies of heterodimeric BMPs and mixed receptor chains are possible [24] A B Molecular recognition The structures of the complexes provide a wealth of information on the mechanism of cytokine...
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Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc
Ngày tải lên :
16/03/2014, 01:20
... studies of the reaction mechanism of rat liver phosphoglycerate kinase in the direction of ADP utilization Biochimie 65, 211–220 36 Geerlof A, Travers F, Barman T & Lionne C (2005) Perturbation of ... sequences analyzed The analysis of the predicted structure suggested that the presence of the shorter lateral chain of Val311 instead of Leu allows for the movement of Tyr239 and Glu309 towards ... varying concentrations of GTP or ATP, in the presence of saturating concentrations of the co-substrate 3-phosphoglycerate (6 mm); the reaction was started by adding 0.4–2 lg of enzyme For Km value...
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Báo cáo khoa học: Molecular basis of cerebral neurodegeneration in prion diseases pdf
Ngày tải lên :
30/03/2014, 09:20
... significantly increased generation of Ctm PrP In addition, several pieces of evidence have linked CtmPrP to neurodegeneration in transgenic mice Toxicity of transmembrane isoforms of PrP The evidence that ... deletion of the internal HD could change a neuroprotective activity of wildtype PrP to the pro-apoptotic activity of mutants, such as PrPDF The HD might directly mediate an interaction of PrPC ... Regardless, the exact mechanism of the study clearly emphasizes a critical role of the GPI anchor of PrP in the pathogenesis of prion diseases No central nervous system toxicity of PrP missing the GPI-anchor...
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Báo cáo khoa học: The molecular basis of heme oxygenase deficiency in the pcd1 mutant of pea pot
Ngày tải lên :
30/03/2014, 11:20
... contribution of these assay components by omitting them individually In the absence of ferredoxin the reaction rate decreased to 46.5% of the complete reaction while the absence of ascorbate ... region of Arabidopsis thaliana HO1 (AtHO1) and was used as the basis for the design of gene specific primers The 5¢- and 3¢-ends of the pea HO1 cDNA were obtained by rapid amplification of cDNA ... complete reaction was estimated as 63.3 nmol BV IXa h)1Æmg protein)1 with a Km for heme of 3.1 lm When the assays were performed in the absence of Table Effect of assay components on activity of PsHO1...
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THE MOLECULAR BASIS OF PLANT GENETIC DIVERSITY pdf
Ngày tải lên :
28/06/2014, 11:20
... assessments of the conservation status of all plant species, in order to prioritize those in need of conservation action and to provide a measure of the success of actions being taken The improvement of ... Preface The purpose of The Molecular Basis of Plant Genetic Diversity is to provide a glimpse into the dynamic process of genetic variation by presenting the thoughts of some of the scientists ... estimated fraction of all individuals that would be 20 The Molecular Basis of Plant Genetic Diversity heterozygous for any randomly chosen locus It is often calculated based on the square root of the...
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Báo cáo sinh học: "X-chromosome inactivation: the molecular basis of silencing" ppt
Ngày tải lên :
06/08/2014, 18:21
... cell Xist Tsix The role of DNA methylation Figure Transcription of Xist and Tsix on the X chromosome The coding sequences of Xist and Tsix overlap on opposite strands of the X-chromosome DNA ... initiated A second cause of non-random X-chromosome inactivation is the selective death of cells that inactivate the incorrect number of X chromosomes: because the fates of the X chromosomes are ... regulates many aspects of gene expression and involves the production of antisense RNA complementary to sequences in the mRNA of the gene that is being regulated [24] The formation of sense-antisense...
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Báo cáo y học: "The molecular basis of lung cancer: molecular abnormalities and therapeutic implications" potx
Ngày tải lên :
12/08/2014, 18:21
... subclasses of adenocarcinomas Selected genes allow the discrimination between primary lung cancer and metastasis of extrapulmonary sites [90] Studies of expression profiles of adenocarcinomas of the ... Tissue microarrays (TMAs) of lung cancer Tissue microarrays (TMAs) of lung cancer TMAs are comprised of core biopsies of 0.6 mm in diameter of different tumors and of uninvolved lung from the ... presented mean (n = 4) ± coefficient of variance (CV=STD/Mean) B View of Chromosome array CGH profile on the same squamous carcinoma of the lung showing the size of the amplicon Human cytogenetic...
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Báo cáo y học: "Application of the comprehensive set of heterozygous yeast deletion mutants to elucidate the molecular basis of cellular chromium toxicity" potx
Ngày tải lên :
14/08/2014, 08:20
... [20] The proof -of- principle of competition analyses employing the heterozygous yeast deletion mutants involved confirmation or identification of essential proteins as targets of drug action [14,15,21] ... resistance of the cell to the toxic action of the metal Over-representation of specific Gene Ontology terms in the annotations of genes found in the haploinsufficiency and haploproficiency datasets ... 100 50 -0.2 -0.05 0.05 0.3 Size of growth effect Figure of Analysis the global effects of Cr treatment on the heterozygous mutants Analysis of the global effects of Cr treatment on the heterozygous...
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Báo cáo y học: "Molecular basis of telaprevir resistance due to V36 and T54 mutations in the NS3-4A protease of the hepatitis C virus" pdf
Ngày tải lên :
14/08/2014, 08:20
... previous page) Network of non-covalent residue interactions for the NS3-4A protease and the corresponding list of protein-ligand interactions Network of non-covalent residue interactions for the NS3-4A ... analysis of MD simulation results, the average structures of the protease-inhibitor complexes of the last 100 ps of the simulations were used, superimposing backbone Cα atoms On the basis of these ... SCH 446211 Molecular structures of the NS3-4A serine protease inhibitors VX-950 (telaprevir) and SCH 503034 (boceprevir) as well as of the co-crystallized protease Molecular structures of the NS3-4A...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 5
Ngày tải lên :
17/09/2015, 17:20
... mammalian cells Nonetheless, the precise mechanism of gene transfer is still unclear It will be necessary to identify the specific endogenous factor(s) of A tumefaciens required for the efficient mammalian ... high DNA transfer efficiency of AG6 strain might allow us to develop an efficient human cell gene delivery or a good vehicle for DNA vaccination The feasibility of using this system for DNA delivery ... in the expression of acidic pH-inducible genes, but the mechanism is still unclear Detailed studies is necessary in order to get more information for a better understanding of the signal transduction...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 4
Ngày tải lên :
17/09/2015, 17:20
... and/or interactions with cytoplasmic domains of other proteins In many cases (including that of EnvZ), deletion of sensor modules results in a partially or completely active form of the kinase ... output response of the system is determined by the level of phosphorylated RR The genes encoding the sensor and regulator are often cotranscribed as a single transcript Two kinds of phosphorylation ... presence of the appropriate stimulus, to an Asp residue in the N-terminal receiver domain of the regulator protein The phosphorylation of receiver domain may result in a conformational change of the...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 3
Ngày tải lên :
17/09/2015, 17:20
... results indicate that the mechanism of Agrobacterium-plant interaction is different from that of Agrobacterium-mammalian cell interaction 97 3.2.5.2 Role of actin in A tumefaciens internalization ... process of passive uptake of foreign DNA by mammalian cells 3.2.3.3.2 Effect of katA on DNA delivery into mammalian cells katA gene encodes a catalase, which is involved in the dismutation of hydrogen ... RT-PCR According to the instructions of the manufacturer, approximately 10-100 of target molecules should be sufficient for the detection Forty cycles of the reaction was necessary to detect the...
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Molecular basis of agrobacterium mediated gene transfer into mammalian cells 2
Ngày tải lên :
17/09/2015, 17:20
... µl of M NaCl, 500 µl of proteinase K (5 mg/ml), and 500 µl of 10% SDS were add to the cell suspension and then incubated at 68 °C for 30 The lysate was extracted once with 1:1 phenol-chloroform ... (BioRad) was set to the 25-µF capacitor, voltage of 2.5 kV and controller unit of 400 ohms The outside of the cuvette was wiped with tissue paper to get rid of moisture before the cuvette was slide into ... of 400 µl of chloroform, mixing for 30 s, incubation at room temperature for min, and centrifugation for 15 at 12000 × g and °C The RNA in the colorless aqueous phase was precipitated in ml of...
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