modulation of cell fate using nuclear and cytoplasmic extracts pdf

... mediated cell death Of note, apoptosis of fibroblast-like cells is regulated at a number of different levels, and there is evidence that secondary modulation of signalling pathways downstream of Fas ... known about the molecular and cellular basis of such alterations Tenocytes are specialized, fibroblast-like cells of mesenchymal origin that constitute the cellular component of periarticular tendons ... of such cells to Fas-induced cell death have been strongly implicated in the pathogenesis of inflammatory joint diseases such as rheumatoid arthritis [10,11] The role of TNF-α in triggering and...

Ngày tải lên: 09/08/2014, 01:23

... 151rom70 and the cell surface were studied using crude virus extracts (Figure 1C) Although the binding of CVB5 to CHO cells was higher using crude extracts, the specific binding to HeLa and recombinant ... low amounts of viruses using this RT-PCR approach By using a fixed ratio of virus/host cell relation (MOI 0.05 TCID50 /cell) , the ability to study interaction using a limited amount of cells was ... their target cells RT-PCR is a rapid and sensitive method suitable for attachment studies and allows the use of crude virus containing extracts as well as limited amounts of cells and viruses...

Ngày tải lên: 12/08/2014, 04:21

... SKW6.4 and H9 cells 67 Fig 3.5 Apoptotic effects of adenosine on SY5Y and MN9D cells 69 Fig 3.6 Apoptotic effects of CCPA on SY5Y and MN9D cells 70 Fig 3.7 Apoptotic effects of CGS21680 on SY5Y and ... by three extracellular and three intracellular hydrophilic loops The number of amino acids comprising the extra- and intracellular loops and the extracellular N-terminal and intracellular C-terminal ... Family of nucleoside transporters 23 Table Effects of extracellular purines on cell growth 38 Table Strategies of the extracellular mechanism studies of AIA in BHK 184 cells Table Strategies of the...

Ngày tải lên: 11/09/2015, 16:07

... Multiplicity of infection Msi-1 Musashi-1 mTOR Mammalian target of rapamycin NF-κB Nuclear factor-kappaB NF1 Neurofibromin NFATc Nuclear factor of activated T cells NG2 Neuroglial chondroitin sulfate ... studies because of its dual role in cell death and cell fate We show that GSK3 regulates GPC maintenance and survival Depletion of GSK3 activity impairs proliferation, triggers apoptosis and reduces ... and proliferation of colon cancer cells both in vitro and in vivo65 In addition, GSK3β inhibition reduces pancreatic cancer cell survival and proliferation66, and studies conducted in hepatocellular,...

Ngày tải lên: 03/10/2015, 21:57

... absence and in the presence of MTX Targeting of vimentin was carried out by lipofecting aODN-VIM into parental HT29 cells causing a decrease of 60% in the expression of vimentin (Fig 6D) whereas random ... interconversions and inhibition of biosynthetic processes after exposure of L1210 leukemia cells to antifolates Experimental and network thermodynamic analyses of the role of dihydrofolate polyglutamylates ... Barcelona, Spain) and 500 ng of each primer, and 20 mm Tris ⁄ HCl, pH 8.5 To avoid unspecific annealing, cDNA and Taq DNA polymerase were separated from primers and dNTPs by using a layer of paraffin...

Ngày tải lên: 07/03/2014, 16:20

... respectively the real and measured angles between ~ and the vertical and u the angle between the normal of the facet and the q CCD plane tions of Ross et al in [5] which show that only one out of two sides ... sidewall morphology and Ross et al [5] with saw-tooth faceting They interpret this faceting term of both the role of the geometry and surface energy of the wire and the liquid droplet, and report that ... provide evidence of the hexagonal cross-section of the wires The angle between two streaks is 60° and the directions of the six sides of the nanowires can be deduced from the directions of these streaks...

Ngày tải lên: 16/03/2014, 15:37

... frequently in the role of subject, and codes of position (from 100 to 109), codes of place (from 700 to 709) and codes of building (from 940 to 949) appear most often in the role of object For each ... example, 26.8% of arguments of the verb ttenata (leave) are used as subjects, and 54.4% are used as objects, but 74.41% of antecedents of the verb are of subject role, and 6.9% are of object role ... strength of code frequency f at Lt, and represents the standard deviation above the average of frequency fave,t Referring to Smadja's definition (Smadja, 1993), the standard deviation at at Lt and...

Ngày tải lên: 17/03/2014, 07:20

... to the conception and design of the study, acquisition of data, analysis and interpretation of data, and drafting and revision of the manuscript She has given final approval of this version for ... incorporate prospective measurement of pain and examination of concurrent and predictive associations of pain with HRQOL to improve our understanding of mediating and potentially reciprocal associations ... contributed to the conception and design of the study, acquisition of data, analysis and interpretation of data, and revision of the manuscript He has given final approval of this version for publication,...

Ngày tải lên: 18/06/2014, 19:20

... OpenGL command, we often omit the interface handle Here we set the physical size of frame and make its contents visible Here the physical size corresponds to the number of pixels in x and y direction ... a high-level understanding of all basic graphics principles without some detailed low-level implementations The emphasis is on understanding graphics and using JOGL instead of implementing a graphics ... Scan-conversion, and Clipping generate a random line with -1

Ngày tải lên: 03/07/2014, 16:08

... Applications of LFSRs include generating pseudo-random numbers, pseudo-noise sequences, fast digital counters, and whitening sequences Both hardware and software implementations of LFSRs are ... count from to 77 on a negative edge of the clock and then restart at ECE574: VHDL and Verilog Modeling and Synthesis 12/08/10 Question [20 marks]: An FPGA running off its own local 100MHz oscillator ... after an access time of tacc from OE and CE Write operation (CE and WE valid - write data on the rising edge of WE) In the model check that the address bus is stable for taddr and the data bus is...

Ngày tải lên: 11/07/2014, 02:21

... sets of data before fusing and forming a new set of data DWT is one of popular tools used in MRA by using low-pass and high-pass filter [6, 7, 8] Consider is DWT performance, the analysis and ... 10 Histogram and standard deviation comparisons (a), (c), (e) are histograms and standard deviation corresponding to R, G, B color of image 9a; (b), (d), (f) are histograms and standard deviation ... 11 Histogram and standard deviation comparisons (a), (c), (e) are histograms and standard deviation corresponding to R, G, B color of image 9b; (b), (d), (f) are histograms and standard deviation...

Ngày tải lên: 22/07/2014, 02:21

... are given in roman type and gene names in italics †In unpublished experiments, accumulation of actin and disruption of the cell monolayer was found with profilin RNAi and a cytokinesis phenotype ... protein, Cdc42, cofilin, cofilin phosphatase, cyclase-associated protein, profilin and Scar The screens did not agree on whether lamellar or cell- shape defects were found with the depletion of the following ... revealed phenotypes such as changes in cell shape or the accumulation of multinucleated cells upon depletion of only 160 of the RNA targets (one third of which correspond to a gene lacking a...

Ngày tải lên: 06/08/2014, 18:20

... RNAi targeting of members of a different group of genes resulted in a profound loss of actin filaments in both cell types, identifying known regulators of F-actin formation In Kc167 cells, dsRNAs ... morphology of Kc167 and S2R+ cells, respectively (Figure 1a-c) By labeling actin filaments, microtubules and DNA, it was possible to assay a wide range of cellular behaviors in these cell types, ... an increase in cell size and an altered DNA morphology, indicative of growth in the absence of division A second group (Profile II) was defined by an increase in the frequency of cells with a microtubule...

Ngày tải lên: 06/08/2014, 18:20

... are specific for the different phases of the cell cycle and determine the targets of the kinases Exit from each phase of the cell cycle occurs on degradation of the bound cyclin The CDK-cyclin complex ... Khodjakov and Rieder: Journal of Biology 2009, 8:88 88.5 use because fluorescence-activated cell sorting (FACS) cannot distinguish 4N G2 cells from 4N mitotic cells (or even 4N G1 cells that ... flies Cell 2008, 133:1032-1042 Clute P, Pines J: Temporal and spatial control of cyclin B1 destruction in metaphase Nat Cell Biol 1999, 1:82-87 Gascoigne KE, Taylor SS: Cancer cells display profound...

Ngày tải lên: 06/08/2014, 19:21

... streptococci investigated 94 and of the strains fermented inulin and raffinose, respectively Additionally, 130 of the investigated strains, and the S uberis reference strains NCDO 2038 and NCDO 2086 showed ... al Fig Typical fragments of the PCR amplified 16S rRNA gene of S uberis (1, 2, and 3) and S parauberis (4, 5, and 6) after digestion with the restriction enzymes RsaI and AvaII, respectively M ... approximately 140, 190, 380 and 700 bp AvaII restriction revealed three different fragments with sizes of 230, 310 and Fig Amplicons of S uberis (1, 2, 3, 4) with a size of 340 bp using the S uberis 16S-23S...

Ngày tải lên: 07/08/2014, 17:22

... The cell suspension was washed and viable chondrocytes counted using a haemocytometer and trypan blue Cells were finally resuspended in medium at a cell concentration of × 106 cells.ml-1 using ... periods of compression resulted in a greater magnitude of stimulation of sGAG content and expression of collagen type II and fibronectin even in the presence of fragments The importance of these ... expression of matrix proteins and Page 13 of 15 downregulation of cytokines and MMPs, implicating NO dependent pathways The ability of chondrocytes to interact with matrix fragments and respond...

Ngày tải lên: 12/08/2014, 12:20

... calculated as: *(No of rolling cells) × 100)/(No of rolling cells + No of firmly adherent cells) Mean rolling velocities were determined from more than 25 individual velocity profiles for each experimental ... contributions AP and BN conceived of the study, participated in its design and coordination, and drafted the manuscript VS and AH carried out the adhesion assays JR and HAH participated in the design of the ... setting Additionally, the use of cell suspensions instead of whole blood influences rheological properties and the fixed diameter of the flow channel precludes effects of luminal narrowing that may...

Ngày tải lên: 13/08/2014, 21:21

... preparation and hybridization, S Dow for reagent and primer handling, and E Coffey and the Array Production Team for array synthesis We also thank M Krolewski and S Ezekiel for database and programming ... predictions were run on six-frame translations of the PTIs using the HFRAME software from Paracel with an E-value cutoff of 0.01 and frameshift penalty of -12 Information on Pfam [31] domains is ... or not on the basis of the log of the Mahalanobis distance and an approximation of the diagonal distance (slope) of the probe from the minimum first PCS and median second PCS Using these measures...

Ngày tải lên: 14/08/2014, 14:21

Ngày tải lên: 04/10/2014, 22:10