modifying an element from the input document

... derived from the maximum velocity (V), divided by the molar concentration of the enzyme (e0, included in Vmax) R and T are the gas constant and the temperature (in Kelvin), respectively The values ... Molecular and biochemical characterisation of the thermo-active family pectate lyase from the hyperthermophilic bacterium Thermotoga maritima Biochem J 370, 651659 21 Kozianowski G, Canganella F, ... Canganella F, Rainey FA, Hippe H & Antranikian G (1997) Purication and characterization of thermostable pectate lyases from a newly isolated thermophilic bacterium Thermoanaerobacter italicus sp nov...

Ngày tải lên: 20/02/2014, 03:20

... sodium hydrogen carbonate, and L -glutamine purchased from Biochrom (Berlin, Germany) Other chemicals were from Sigma unless otherwise stated Cell culture and treatment The human hepatocarcinoma line ... encompassing the putative SBE from the SeP promoter Lane contains nuclear extracts from untreated HepG2 cells, lane and include extracts from cells treated with 100 pM TGF-b1 for 30 and 60 min, ... min, respectively Lane depicts the protein preparation from lane containing 500-fold molar excess of unlabelled probe Lane 5, and each contain 500 ng of anti-Smad 2, and 4-specific antibodies, respectively,...

Ngày tải lên: 31/03/2014, 23:20

... read and agree with the manuscript RS drafted the protocol, sought ethical approval, collected data, helped in analysis and drafted the manuscript SP helped collect data, analyse and draft the manuscript ... financially from the publication of this manuscript, either now or in the future The authors not hold any stocks or shares in an organization that may in any way gain or lose financially from the publication ... drafting the protocol, collecting data, analysis, statistics and drafting the manuscript SJS helped draft the protocol and prepare the manuscript Competing interests The authors state that they have...

Ngày tải lên: 20/06/2014, 04:20

... read and agree with the manuscript RS drafted the protocol, sought ethical approval, collected data, helped in analysis and drafted the manuscript SP helped collect data, analyse and draft the manuscript ... financially from the publication of this manuscript, either now or in the future The authors not hold any stocks or shares in an organization that may in any way gain or lose financially from the publication ... drafting the protocol, collecting data, analysis, statistics and drafting the manuscript SJS helped draft the protocol and prepare the manuscript Competing interests The authors state that they have...

Ngày tải lên: 20/06/2014, 07:20

... DIF, as well as an ANOVA of the residuals Thus this DIF based ANOVA analysis is subtly different from the distributional analysis of the ANOVA approach which preceded it, as the latter does not ... contributions AK, SK and AT were involved with the conception and design of the study AK and SK arranged the data collection, took part in the interpretation of the data, and the Page of (page number ... where clinical or other cut points are established by other means, if they fail to meet the requirements of the absence of DIF by relevant groups, then the unidimensionality of any summative score...

Ngày tải lên: 20/06/2014, 15:20

... purposes) Health and Quality of Life Outcomes 2006, 4:55 An analysis of the clinical meaning of the DIF problem was then conducted by testing whether the meaning of the summed score reflected the same ... assistance or the subject expends >75% of the effort, moderate assistance or the subjects expends 50 to 74% of the effort, and maximal assistance or the subject expends 25 to 49% of the effort The ... also given to the Norwegian centres and Denmark The centres did however not have to state which version of the manual was used, however the manuals are quite similar Rasch analysis The Rasch model...

Ngày tải lên: 20/06/2014, 15:20

... requires adjustment for the probable phenomenon that the more severe the disease, the more intensive the therapy The question can conversely be turned on its head, and the data can be used to address ... development of anti-CCP antibodies [34] Further publications have confirmed an association between PADI4 and RA in another Japanese population [35], in a South Korean population [36] and in a North ... between the PADI4 gene and RA [33] We were not able, however, to find any association between polymorphisms of the PADI4 gene and the presence, the extent or the progression of erosions or the development...

Ngày tải lên: 09/08/2014, 08:22

... vocabulary On the one hand, it gives values to the address; on the other hand, it enlarges social distance between the speaker and the hearer; which is, in this context, distance between the country-leader ... best, value the life we see in one another, and must always remember that even the unwanted have worth The President describes all the losses American have to bear and the expenses the state budget ... borders, and raise a mortal threat(7) There is only one force of history that can break the reign of hatred and resentment, and expose the pretensions of tyrants, and reward the hopes of the decent and...

Ngày tải lên: 18/12/2013, 10:08

... determine whether the s-DAPK-1 expression occurs in normal human tissue as well as primary human cancers, rather than just cell lines and cDNA from the NEDO human sequencing project We evaluated the expression ... in the human genome might be found that crosstalk to the DAPK-1 pathway Therefore, we evaluated the homology of the ankyrin-repeat region of DAPK-1 with other genes in the human genome using the ... Flag–s-DAPK-1- and the Flag–s-DAPK-1-Myctransfected cells, whereas the upper band in the Flag– s-DAPK-1 transfection lane was slightly smaller (Fig 2C, lane versus lane 1) This suggests that the depletion...

Ngày tải lên: 18/02/2014, 17:20

... ethylcellulose and Avicel were obtained from SigmaAldrich, and the cello-oligosaccharides were obtained from Merck (Darmstadt, Germany) The oligosaccharides derived from b-glucan were a gift from A Planas ... structural basis for the difference between an exoglucanase (OXG-RCBH from Geotrichum) [14] and an EG (XGH74A from Clostridium thermocellum) [7] Both enzymes have an active site located in an open cleft, ... a wide range of proteins, including other GHs (clans E, F and J) [3] and hemopexin [20] The CD spectrum of the latter protein [21] is very similar to the one observed here; therefore, the unusual...

Ngày tải lên: 19/02/2014, 05:20

... arrived from Guetersloh in their place, and then the departure of the French began The leave-taking between the French and English was very cordial and annoyed the Germans very much, as while the ... between the English and the allies were always of the best About half the English preferred the Russians, while the other half preferred the French There were many amusing incidents constantly ... backwards and forwards Eventually the Russians went and many of us were very sorry to lose them, as it meant a loss of all means of continuing to learn the language from their Russian friends...

Ngày tải lên: 20/02/2014, 08:20

... than 75% of the total (Fig 3B) The diameter obtained from the first maxi˚ mum, 112 A, is in general agreement with the maximum pair distance obtained from SAXS results The ˚ second maximum of the ... ovorubin, the major egg lipoprotein from the apple snail Mol Cell Biochem 243, 9–14 10 Palozza P & Krinsky NI (1992) Astaxanthin and cantaxanthin are potent antioxidants in a membrane model Arch ... ovorubin is within the pH range of vertebrate digestive tract fluids [30,31], and the present results indicate that the protein can withstand the combined effect of low pH values and enzymatic attack...

Ngày tải lên: 07/03/2014, 06:20

... analysis The 105 000 g supernatant of lungfish liver (lane 1), skeletal muscle (lane 2), intestine (lane 3), lung (lane 4), brain (lane 5), adipose tissue (lane 6), heart (lane 7) and skin (lane ... filtration showed that the protein is in a monomer–dimer equilibrium and that the dissociation constant is in the micromolar range for the apoprotein and in the submicromolar range for the holoprotein, ... with the presence of two EF-hand motifs The affinity constants determined agree with the fact that S100 protein affinity for Ca2+ is low, the affinity of the C-terminal EF-hand being greater than...

Ngày tải lên: 08/03/2014, 22:20

... characterize ferritin from an Antarctic fish and thereby establish whether cold adaptation affects the structural–functional properties of this protein Ferritin extracted from the spleen of the Antarctic ... and 4.0 The observed transitions were irreversible, and the spectra measured at the end of the cooling phase were different from those of the native apoprotein The midpoints of the transitions ... to human L chains The most similar to the T bernacchii constitutive chain are the H2 chain from S gairdneri (87.5%) and the M chains from S salar (86.9%) and Gillichthys mirabilis (78.7%) The percentage...

Ngày tải lên: 08/03/2014, 23:20

... present in the shewasin A amino acid sequence The Asp of the active site motif from the N-terminal domain (marked with an asterisk) was mutated to an Ala to generate the active site mutant shewasin ... kDa), and aprotinin (6.5 kDa) (Paris, France) The last of these peptides was found to be cleaved readily, and the rate of hydrolysis was monitored at an excitation wavelength of 328 nm and an emission ... as fraction (D) SDS ⁄ PAGE analysis of protein fractions collected from the different steps of purification Lanes and 2: fractions and in (A) Lane 3: fraction in (B) Lane 4: Superose 12 eluate...

Ngày tải lên: 14/03/2014, 22:20

... The thermostable T elongatus Dps S Franceschini et al the CD loop, the beginning of the D helix and the last part of the B helix It is stabilized by hydrophobic and hydrophilic interactions and ... the C-terminal tail, the last part of the D helix and the first B helix residues (Fig 2B, panels and 2) The hydrophobic residues buried most deeply at the interface are Val95, on the C helix and ... and 282 nm due to 1Lb vibronic transitions of the Trp34 and Trp144 residues, and a negative peak at 297 nm due to 1La vibronic transitions In the other members of the Dps family the tryptophan...

Ngày tải lên: 16/03/2014, 12:20

... investigated the influence of the ARE and the CRID on the poly(A) status of the human IFN-b mRNA in natural conditions of IFN-b synthesis Materials and methods Reagents All the reagents and enzymes ... addition, the sequence encoding the HA epitope was inserted at the end of the IFN-b coding sequence to distinguish the products resulting from the expression of the DNA constructs and the endogenous ... RNAse H in the presence of oligo(dT) before the Northern blot analysis with a HA antisense riboprobe As shown in Fig 3A (lanes and 7), the HA-tagged IFN-b transcript underwent significant deadenylation...

Ngày tải lên: 17/03/2014, 10:20

... in the N-terminal strand and three in the C-terminal strand This leads to a higher twist of the b sheet of androctonin compared to the two other peptides Despite such differences, the rmsd of the ... in the headgroup region of the membrane, leading to a decrease of the thickness of the lipid bilayer, and then to (b) an active state, when the peptide penetrates the hydrocarbon core of the ... [44] To obtain a better understanding of the importance of disulfide bridges and the hydrophobic-hydrophilic balance on the antimicrobial activity of gomesin, synthetic analogues of this peptide that...

Ngày tải lên: 17/03/2014, 23:20

... reviewing and addressing at all There is more than one way to mitigate a given risk, and the best risk managers are the ones who can get to the root of the problem and find a creative way to limit the ... them If they are spending all their time with enforcement, then either the educational aspects of the program are failing or they don’t have the necessary support from the leaders in the organization ... want your organization’s resources to be used in an attack on another organization Even if you can’t prevent a resource from being exploited, it is important to provide the means to recover from...

Ngày tải lên: 23/03/2014, 03:20