modification of cellular adhesion strength by cytoskeletal mutations

... expression of vascular cell adhesion molecule-1 (VCAM-1) [7], intercellular adhesion molecule-1 [8] and P-selectin [9] both in vitro and in vivo However, the intracellular signal pathway of these ... 1200 800 + - + + - + + Fig Abolition of LPC induction of AMs by cotransfection of ACREB The involvement of CREB in LPC-induced AM activation was evaluated by cotransfection with the CREB dominant ... - + + - + + + Fig Enhancement of LPC-induced AM activation by transfection of GPR4 The effect of GPR4 on LPC-induced AM activation was evaluated by transfection of human GPR4 expression vector...

Ngày tải lên: 30/03/2014, 09:20

... 49.2 Journal of Biology 2009, Volume 8, Article 49 Suzanne and Steller adjacent cells initiate the assembly of an adhesion complex by homophilic binding of their extracellular domains β-Catenin ... how the event of cellular detachment is transmitted to the core apoptotic machinery Finally, whereas the study by Kessler and Muller [9] focuses on the regulation of cell adhesion by caspases, ... be at least partially rescued by amino-terminally truncated Arm An alternative is that modifications of other components of the adherens junction complex (cleavage of E-cadherin has been reported...

Ngày tải lên: 06/08/2014, 19:20

... (QPCR) genes of transgenes on the expression of hostanalysis of the effects Real-time Real-time quantitative RT-PCR (QPCR) analysis of the effects of transgenes on the expression of host genes ... infection of tomato by severe strains of PSTVd reduced pollen viability [41] Symptoms of viroid infection in tissue culture are varied but include specific inhibition of root formation by HSVd ... http://www.biomedcentral.com/1471-2229/9/108 A B Figure Morphology of flowers and pollen from of transformants Morphology of flowers and pollen from of transformants (A) Over expression of pkv results in reduced flower size...

Ngày tải lên: 12/08/2014, 03:21

... inflammation of New Zealand rabbits There are several strengths in this study First, we used MTP model to identify the significance of a group of candidate genes while we examined effect modification by ... Different findings of effect modification by GSTM1 variation across studies may reflect differences of exposure, outcome and population, measurement errors in exposure or phenotype, and by chance Similar ... variants of the significant genes identified by MTP with significant interactions using the bootstrap method with the combination of coefficients of the main effect and the interaction [6] Page of...

Ngày tải lên: 17/02/2014, 22:20

... important For example, rearrangement of the cytoskeleton and concurrent activation or inhibition of plasma membrane solute transporters are often prominent features of cell-volume regulation, and specific ... cells [18] We began our studies by reconstitution of recombinant CLIC5 in the planar bilayers, and set out to test the hypothesis that membrane CLICs are regulated by cytoskeletal actin, after functionally ... formed by CLIC1 and CLIC5 were directly and reversibly regulated by F-actin, without an intermediate molecule or adaptor protein In contrast, the ion channels formed by CLIC4 were not regulated by...

Ngày tải lên: 30/03/2014, 04:20

... in the presence of a μg/ ml concentration of HBsAg Following addition of RNAlater, total cellular RNA was extracted, reverse transcribed and amplified by qRT-PCR in the presence of primers and ... axes) Page of (page number not for citation purposes) Journal of Translational Medicine 2008, 6:58 However, the correlation of specific profiles of cytokine gene expression with markers of protection ... cellular RNA was extracted by using Ribo PureBlood kit (Ambion Inc., no AM1928, Austin, TX, USA) and eluted in 75 μl of elution buffer Reverse transcription was done with 11 μl of total RNA by...

Ngày tải lên: 18/06/2014, 15:20

... Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 domains revealed the three genotypes share the same conserved amino acid 21–30 BH3 domain is characterized by ... pathway which is governed by Bcl-2 family of proteins is involved in the development of liver diseases [9,10] The Bcl-2 family of proteins is defined as the key regulator of apoptosis in the mitochondria-dependent ... The transfected cells showed characteristics of cellular apoptosis supported by FACS analysis Further investigation identified the natural expression of HBSP in HBV genome transfected cells The...

Ngày tải lên: 18/06/2014, 18:20

... Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 domains revealed the three genotypes share the same conserved amino acid 21–30 BH3 domain is characterized by ... pathway which is governed by Bcl-2 family of proteins is involved in the development of liver diseases [9,10] The Bcl-2 family of proteins is defined as the key regulator of apoptosis in the mitochondria-dependent ... The transfected cells showed characteristics of cellular apoptosis supported by FACS analysis Further investigation identified the natural expression of HBSP in HBV genome transfected cells The...

Ngày tải lên: 20/06/2014, 01:20

... greater adhesion force than unfixed cells h Comparison of adhesion force of control groups and testing groups, indicating the adhesion force of nonincubated cells (control I) is smaller than that of ... variation of approaching portion of force curve (black double-head arrow in a), the changes of cell stiffness can be preliminary determined g Histograms of statistical results of surface adhesion ... evaluated the effect of fixative (glutaraldehyde) and cell isolation process on the adhesion force of cell membrane, indicating the fixative resulted in the increases of adhesion force of cell membrane,...

Ngày tải lên: 21/06/2014, 20:20

... medium consisting of TCM 199, 10% SS, 50 μM of cysteamine and 0.02 U of porcine follicle stimulating hormone/ml Oocytes in groups of 5∼10 were transferred to 50 μl droplets of culture medium ... small volume of vitrification solution The rest of the straw was filled with a dilution medium consisting of 0.5 M sucrose in base medium that was separated by air bubbles from the column of vitrification ... developmental competence of buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification Post-thaw in vitro developmental competence of the embryos vitrified after cytoskeletal stabilization...

Ngày tải lên: 07/08/2014, 23:22

... and by further 10 of (left) and incubation ation with single doses between h pretreatment with genistein24 hμM hormonal with estradiol 10 μM (right), followed by irradiSurvival of Survival of ... result reflects independent assays Proportion of cells in G0/G1 is symbolised by a bar, G2/M by a white bar and S-phase by a black bar In the controls 15% of the cells were in S-phase, 65% in G0/G1 ... by the missing of functional p53, thus lacking of any G0/G1 arrest The G2/ M arrest after irradiation with high doses was not abolished by estrogenic stimulation These results are supported by...

Ngày tải lên: 09/08/2014, 09:22

... interactions Figure of Groups phyletic patterns and COGs revealed by hierarchical clustering of patterns in species space Groups of phyletic patterns and COGs revealed by hierarchical clustering of patterns ... percentage of lost data (b) The effect of selected distance measures between phyletic patterns on the recovery of functionally linked pairs of genes The criteria of functional linkages on the basis of ... algorithm We investigated the effect of various distances between patterns, of different clustering approaches, and of several methods of tree splitting on the recovery of functionally linked proteins...

Ngày tải lên: 09/08/2014, 20:20

... proteins are located in nucleus and membrane Validation of cellular proteins by Western blot Following the identification of cellular proteins by proteomic method, immunoblot analysis was carried ... cleavage site, partial modification of cysteine carboamidomethylated and methionine oxidized, none fixed modifications, MS tolerance of 60 ppm, MS/ MS tolerance of 0.25 Da A total of 133,518 sequences ... purified PRRSV virions by 2-DE coupled with MALDITOF/TOF MS proteomic approach In addition, we utilized Western blot and immuno-gold labeling assays to verify the presence of cellular proteins and...

Ngày tải lên: 12/08/2014, 01:22

... of a PstI site in S7 of BTV8, and the absence of a BglII site in case of S7 of BTV6 (Table 1, and Figure 2, lane and 9) Furthermore, the sequence of this segment S7 was 100% identical to S7 of ... sequence of the T7 promoter is italicized, and the BsmBI site is underlined The positions of the start of transcription and digestion by restriction enzymes for run-off transcription are indicated by ... to be useful to generate mutants of BTV by genetic manipulation of one or more of genome segments [15] This system needs, however, a set of ten complete cDNAs of genome segments to rescue bluetongue...

Ngày tải lên: 12/08/2014, 01:22

... Chemistry of dendrimers 1.3.2 Biological application of dendrimers 12 1.3.3 Cytotoxicity of dendrimers 14 1.4 Chemical modification of mammalian cellular membrane 14 1.4.1 Insertion of molecules ... Overview of cellular membrane 1.1.2 Lipid domain and its charge 1.2 Cholesterol and cellular membrane 1.2.1 General feature, location of cholesterol within cellular membrane 1.2.2 Roles of cholesterol ... biological profile of a dendrimer-based delivery system (with surface modifiers and a payload of drug) is likely to be different from that of an unmodified dendrimer.89 1.4 Chemical modification of mammalian...

Ngày tải lên: 06/10/2015, 21:28

Ngày tải lên: 04/10/2012, 10:02

Ngày tải lên: 04/10/2012, 10:02

... using Lipofectamine (Invitrogen) and PLUS reagent (Invitrogen) Expression of GFP/NS1 or GFP was induced by the addition of 10 µg/ml ponasterone A (Invitrogen) Protein expression was monitored by fluorescence ... possibility suggested by the finding of NS1-DNA attachment concerns the development of autoantibodies after B19 infection B19 infection often results in the production of anti-DNA autoantibodies ... Christensen J, Rommelaere J Initiation of minute virus of mice DNA replication is regulated at the level of origin unwinding by atypical protein kinase C phosphorylation of NS1 J Virol 2001;75(13):5730-9...

Ngày tải lên: 25/10/2012, 11:18