modern instrumental techniques third edition

... large value of S), and observed peak widths for viruses are similar to those for proteins. The third consequence of viral size is their large hydrodynamic volume, which restricts their entry ... preparative tool to isolate biologically active species (often in concert with other chromatographic techniques in a multi-stage purification pro- cess), or as an analytical tool to obtain information

Ngày tải lên: 04/07/2014, 01:20

... 731–733 Sample peak capacity; see Equivalent peak capacity Sample preparation, 66, 757–804 affinity techniques, 800 biochromatography, 797–800 column switching, 796–797 derivatization, 194, 802–804 ... 770 solute binding, 769 solvent selection, 767 special approaches, 770–771 theory, 766 membrane techniques, 790 908 INDEX Sample preparation (contd.) overview, 758–759 particle-size reduction, ... symptoms; see Troubleshooting symptoms system suitability, 813 tables, 865–888 TFA, 817 tips and techniques, 814–819 water purity, 817–818 Troubleshooting symptoms, 821–888; see also Troubleshooting...

Ngày tải lên: 04/07/2014, 01:20

... second edition of this book appeared in 1979, and for tens of thousands of readers it eventually became their choice of an HPLC reference book The remarkable staying power of the second edition ... the second edition, today reversed-phase chromatography (RPC) accounts for about 80% of all HPLC applications— and therefore receives major (but not exclusive) attention in the present edition Over ... carried out have mandated major changes in the present edition The organization of the present book, while similar to that of the second edition, has been significantly modified in light of subsequent...

Ngày tải lên: 04/07/2014, 01:20

... are equipped for HPLC; it is often the first choice of technique Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R Snyder, Joseph J Kirkland, and John W Dolan Copyright © 2010 ... of the most useful and widely applied analytical techniques Mass spectrometry rivals and complements HPLC in many respects; the use of these two techniques in combination (LC-MS) is already substantial ... colorimetric reagents to the plate, after the separation was completed HPLC (Fig 1.1g, h) represents the modern culmination of the development of liquid chromatography The user begins by placing samples...

Ngày tải lên: 04/07/2014, 01:20

... the early 1960s of a gel-permeation chromatograph by Waters Associates [16] Each of these latter techniques was close in concept to what later became HPLC, differing little from the schematic of ... had been published [25], and an HPLC short course was offered by the American Chemical Society (Modern Liquid Chromatography), with J J Kirkland and L R Snyder as course instructors) Progressive ... biographies of several HPLC practitioners [34] 1.3 SOME ALTERNATIVES TO HPLC Two, still-important techniques, each of which can substitute for HPLC in certain applications, existed prior to 1965:...

Ngày tải lên: 04/07/2014, 01:20