model blood collection and peripheral blood mononuclear cell isolation

... polymorphonuclear (PMN) leukocytes, and a population of CD11b- mononuclear cells T cell- induced inflammation is accompanied by increases in all of these cell types but especially in cells with the phenotypic ... II- cells showed that greater than 80% were PMN cells (not shown) In contrast, the CD45hi cells incorporated the MHC IIhi and MHC IIlo/int subsets and, when sorted, consisted entirely of mononuclear ... similarities in expression of CD11c (75% and 65%), CD54 (70% and 90%), CD172a (60% and 66%), CD11a (60% and 96%), CD32 (50% and 95%), and/ or CD5 (20% and 32%) The two subsets also have similarities with...

Ngày tải lên: 09/08/2014, 10:22

Ngày tải lên: 18/03/2014, 11:20

... bovine serum albumin, and 0.05% Tween (for CXCR2 and CXCR3) The primary antibodies against CXCR1, CXCR2, and CXCR3 were the same as above and used at 1:100 (CXCR1 and CXCR2) and 1:80 (CXCR3) in ... monocytes and T lymphocytes and promotes T cell adhesion to endothelial cells J Exp Med 1993, 177:18091814 Tetlow LC, Woolley DE: Distribution, activation and tryptase/ chymase phenotype of mast cells ... include macrophages and neutrophilic granulocytes, as well as tissue mast cells (MCs) [9,10] Migration of T cells to sites of inflammation is mediated by selectins and their ligands [11,12] Regulation...

Ngày tải lên: 09/08/2014, 01:23

... contributions AS and DD designed this study and carried out immnunohistochemistry staining, western blotting and RT-PCR and drafted the manuscript LM, and KB, provided the clinical samples and collected ... of JAK3/STAT3 in colon carcinoma tumors and cell lines: inhibition of JAK3/STAT3 signaling induces apoptosis and cell cycle arrest of colon carcinoma cells Am J Pathol 2005, 167:969-980 Mora ... Yamauchi-Takihara K, Lin J: Signal transducer and activator of transcription is involved in cell growth and survival of human rhabdomyosarcoma and osteosarcoma cells BMC Cancer 2007, 7:111 Chen SY, Takeuchi...

Ngày tải lên: 10/08/2014, 10:21

... Transcript abundance for MYB genes and secondary cell- wall-related genes in differentiating secondary xylem and in primary Transcript abundance for MYB genes and secondary cell- wall-related genes in ... Transcript accumulation for MYB genes and secondary cell- wall-related genes in differentiating compression wood and oppoTranscript accumulation for MYB genes and secondary cell- wall-related genes in differentiating ... work and the data analysis, participated in the design of the study and drafted the manuscript JGP and JM participated in the design of the study and in manuscript preparation All authors read and...

Ngày tải lên: 12/08/2014, 05:20

... 3β-androstenol sulfate HO 5α-androsten-3α-ol (α-androstenol) hydroxy-androstenone sulfate 3αHSD O androstenone hydroxy-androstenone hydroxysteroid sulfotransferase andro 3-enol sulfate andro 3-enol Figure ... 5,16-androstadien-3β-ol O 4,16-androstadien-3-one Final products of androstenone sulfonation 5α-androsten-3β-ol (β-androstenol) SRD5A 3βHSD 3α-androstenol sulfate hydroxysteroid sulfotransferase 3β-androstenol ... cholesterol CYP21 andien-β synthetase (CYB5, CYP17…) 11-deoxycorticosterone 11- 4,16-androstadien-3-ene 4,16-androstadien- 3βHSD androstadienol sulfate hydroxysteroid sulfotransferase HO 5,16-androstadien-3β-ol...

Ngày tải lên: 14/08/2014, 13:21

... detected for androstenone [21] GENES INVOLVED IN THE METABOLISM OF ANDROSTENONE 3.1 Androstenone synthesis and metabolism Together with other 16-androstene steroids, androstenone (5 α-androst-16en-3-one) ... 5α-androsten-3α-ol α-androstenol 5α-androsten-3β-ol β-androstenol HO 5,16-androstadien-3β-ol andien-β synthetase (CYB5, CYP17…) HO Figure Main enzymes involved in the porcine metabolism of androstenone ... skatole SSC6, SSC13 and SSC14 [21] versus SSC7, SSC12 and SSCX [4] and for indole SSC13 and SSC14 [21] versus SSC2, SSC6 and SSC7 [4] The results of the assessment of lean and fat samples by a...

Ngày tải lên: 14/08/2014, 13:22

... anisomycin and FBS on actin stress fibers in SMCs and their modulation by toxin B and latrunculin B Cells were first stimulated with either S1P, anisomycin or FBS for 30 and then fixed, permeabilized and ... stimulated cells in the absence of inhibitors Fig Effects of RhoA, Cdc42 and Rac1 on the expression of the CTGF/ CCN2 gene (A) Immunoblot analyses of RhoA, Cdc42 and Rac1 activation by S1P and FBS Cells ... containing actinomycin D (10 lgÆmL)1) and the cells were harvested after 0, 0.5, 1, and h Total RNA was purified and analyzed by northern blot hybridization and phosphorimaging densitometry The...

Ngày tải lên: 19/02/2014, 16:20

... lesions on a standard chow diet It is used for studies of hyperlipidemia and atherosclerosis, and has been used extensively in understanding the mechanisms of lipoprotein metabolism and atherosclerosis ... (eWAT, rWAT, and iWAT, left), and brown adipose depots (iBAT, and mBAT, right) by real-time PCR n = *P < 0.05 vs FA Xu et al Particle and Fibre Toxicology 2011, 8:20 http://www.particleandfibretoxicology.com/content/8/1/20 ... ES017412, and ES018900 to Dr Sun, ES015146 and ES017290, and EPA grant R834797-01 to Dr Rajagopalan, an NPACT Initiative grant from the Health Effects Institute to Drs Lippmann and Chen, and laboratory...

Ngày tải lên: 06/03/2014, 19:20

... and WiDr cells COX-1 was highly expressed in HT29 and WiDr cells and weakly expressed in KM20L2 cells COX-2 was detected only in WiDr cells The two terminal PGE2-biosynthetic enzymes, cPGES and ... HEK293 cells and cells transfected with sPLA2-IIA, -IID or -V in response to A23187 (30 min) or IL-1b (4 h) (B) Transcellular PGE2 production by sPLA2-IID (left) and sPLA2-V (right) Control, and ... that by sPLA2-IIA and -V (net 4–5%) (Fig 3A) When cells expressing sPLA2-IID were cocultured with those coexpressing COX-2 and mPGES and then stimulated (transcellular prostaglandin biosynthesis...

Ngày tải lên: 18/03/2014, 01:20

... integrated experimentation and modelling reveal and suggest relations between the changes in morphology, glycolytic flux, ATP production and ATP levels The mathematical models described here have ... other cell types in the brain NO-mediated inhibition of cellular respiration was followed by mitochondrial depolarization and cell death in neurones, but hyperpolarization in astrocytes, and an ... T-046071 and T-067963 to J Ovadi, T-049247 ´ to F.O., and PD 76793 to J Ovadi; FP6-2003-LIFESCIHEALTH-I: BioSim and NKFP-MediChem2 ⁄ A ⁄ ´ 005 ⁄ 2004 to J Olah; and by RET-NORT 08 ⁄ 2004 and ETT...

Ngày tải lên: 23/03/2014, 10:20

... aralar1 and citrin in cells from the immune system and hematopoietic tissues Mitochondrial extracts (20 lg) obtained from human Jurkat cells (a T -cell leukemia cell line) and the mouse macrophage cell ... macrophage-like cell line, the RAW 264 cells (Fig 3B) Aralar1 mRNA and protein were also detected in Jurkat cells and human T-lymphocytes, respectively (Fig 3B and data not shown) and in bone marrow ... dilution Bands correspond to 70 kDa (aralar1 and citrin) and 52 kDa for b-F1ATPase (C) Western blot analysis of aralar1 and citrin in auricular and ventricular myocardium 20 lg of atria (A) and ventricle...

Ngày tải lên: 24/03/2014, 04:21

... estrogentumor cell Ovarian 2receptors lines express STYK1 and variably express Ovarian tumor cell lines express STYK1 and variably express estrogen receptors HS832 is a benign ovarian cell line and the ... benign, and malignant ovarian tissues To investigate the role of estrogen and GPR30 in STYK1 regulation, we treated a benign and several malignant ovarian cancer cell lines with estradiol and G-1 ... cancer cells based on microarray analysis and real-time PCR analysis [10] Estrogen receptors play a critical role in ovarian tumor cell growth Ovarian surface epithelial cells produce estradiol and...

Ngày tải lên: 20/06/2014, 07:20

... [30-32] and a-SiC x :H [33,34], where it has been attributed to a model based on band-tail states In this model, the carriers radiatively recombine with the localized states at the band tails ... the results, and prepared the draft of the manuscript DM analyzed the RBS and ERDA measurement results FD and BSS carried out the PL study MC and FD carried out the TEM studies and investigated ... center) and positively (K+ center) charged silicon atoms as traps for holes and electrons, respectively [48], and third to Si-Si bond or nitrogen vacancy possibly being responsible for electron and...

Ngày tải lên: 21/06/2014, 05:20

... evitcudorper ot eud dlos swoC gnilluc ro ycnangerp litnu ro mutraptsop shtnom fo muminim a rof detcelloc erew atad sredrosid tneirutrapirep fo snoitinifeD tnacifingis deredisnoc erew 50.0< seulav...

Ngày tải lên: 07/08/2014, 18:21

... by the formation of ice within cells and (2) by cell dehydration due to the large difference in the water potential between the unfrozen cell content and the intercellular space, which contains ... detected between I–30, on the one hand, and N and C:N, on the other In January and February, these correlations were even closer in contrast to those of glucose and fructose, whose concentrations ... Block et al (1995) [7] and Gieger and Thomas (2002) [14] Although the overall correlations between N or C:N, respectively, and I–30 were not very tight, and although N and C:N did not contribute...

Ngày tải lên: 08/08/2014, 01:21

... (1 l j − i) and Y = Y (x, zj−i), where z0 = y (7.11) PY satisfies (UD)i in ΓY for all Y ∈ P and i Proof Let x, y ∈ Y and set Z = Y (x, y) ∈ P Then Y ∩ Z belongs to PY and contains x and y, so that ... rank(y) = d and x covers x∧y, there are exactly α+1 pairs (x1 , y1) such that y1 covers both x and x1 , and y covers x1 We say P is regular if P is line regular, dual-line regular and zig-zag ... descendent of H(n, 2) Then n = 2d and one of the following holds: (i) w = and Y = {x ∈ X : wt(x − z) = 1} for some z ∈ 1+ε Xn = Fn \X; (ii) w = d − and there are a ∈ F2 and i ∈ {1, 2, , n} such...

Ngày tải lên: 08/08/2014, 14:23

... White blood cell counts in blood and in synovial fluid were done using an F300 microcell counter (Sysmex, Toa, Japan) Synovial fluid samples were treated with hyaluronidase before the cell count ... recognized TIMPS have been shown to stimulate cell proliferation participating in mitosis and tissue differentiation, to regulate cell survival and apoptosis, and to inhibit angiogenesis The latter ... Samples of blood and synovial fluid from 89 patients with RA were tested for the presence of autoantibodies against all four types of TIMP (TIMP-1, -2, -3, and -4) and compared with the control blood...

Ngày tải lên: 09/08/2014, 06:23

... number of cells (×104/ml) in rheumatoid arthritis and psoriatic arthritis peripheral blood and matched synovial fluid White blood cells Myeloid dendritic cells Plasmacytoid dendritic cells Rheumatoid ... positive - % negative cells] × WBCs per ml [×106/100]) Isolation of pDCs and mDCs from PB and SF Both PB and SF samples were diluted in phosphate-buffered saline and mononuclear cells isolated by ... distribution and phenotype of mDCs and pDCs in blood from PsA patients and RA patients in comparison with osteoarthritis (OA) patients and healthy donors and synovial fluid (SF) from PsA patients and...

Ngày tải lên: 09/08/2014, 07:20

... plates cB -cell clones were cultured in a Millicell culture insert dRA-BMNC cells (3 × 104 cells/well) were inoculated and cultured overnight, and × 106 cells MC/car cells or Molt-17 cells were ... colonystimulating factor, GM-CSF, and the levels of IgM were increased, and IL-1β and TNF were detected Direct contact with the B -cell clone was required for RA-NLCs to produce IL-1β and TNF and higher levels ... pre-B -cell line DW34 A new growth factor was identified, having the ability to enhance DW34 cell growth, and it was designated BST-1 [16] Human BST-1 is expressed in various tissues and cell Available...

Ngày tải lên: 09/08/2014, 08:23