mmtv expression in gr mice

... α-tubulin Figure Gene expression in mammary tumors from MMTV- Wnt-1 versus MMTV- Neu transgenic mice Gene expression in mammary tumors from MMTV- Wnt-1 versus MMTV- Neu transgenic mice (a) Dendrogram ... 0.001) in tumors from MMTV- Wnt-1 transgenic mice Using immunoassays, we demonstrated that keratin and nidogen proteins are expressed at higher level in MMTV- Wnt-1-induced tumors than in MMTV- Neu-induced ... MMTV- Neu-induced tumors described in the preceding section suggest that initiating oncogenes strongly influence gene expression in the tumors arising in these two Genome Biology 2005, 6:R84 information...

Ngày tải lên: 14/08/2014, 14:22

... transgenic mice and the two lines of CYP2E1 transgenic mice, in contrast to WT mice (Fig 3B, C) mRNA expression levels of collagen types III (Col3a1) were increased by two-fold in CYP2E1 transgenic mice ... respectively, in CYP2E1 transgenic mice, and by 18% and 30%, respectively, in cTnTR141W transgenic mice (Fig 4C, D) compared to levels in WT mice These results indicate that the overexpression of CYP2E1 increased ... observed in the WT group between and months of age Death occurred in the cTnTR141W transgenic mice and CYP2E1 transgenic mice beginning at months of age, with respective death rates of 17.5% in cTnTR141W...

Ngày tải lên: 22/03/2014, 16:20

... Betts DM & Grozdanic S (2006) Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons Invest Ophthalmol Vis Sci 47, 1016–1023 Stein I, Itin A, Einat P, Skaliter R, Grossman Z & ... concentration of Ngb in the mouse retina is > 100 lm, and is thus within the range of Mb in the muscle, indicating the important role of Ngb in retinal function [18] However, in contrast to Mb, ... detection and protein level was studied in second order As can be seen in Fig 3, in eyes, during hypoxic incubation, H2O2 production remained at levels comparable with normoxia This finding was expected,...

Ngày tải lên: 23/03/2014, 09:21

... diluted in phosphatebuffered saline (pH 7) Mice in three of the four groups were intravenously injected in the tail vein with 13, 130, and 260 kBq 131I, respectively, while the mice in the control group ... associated with it were included in this categorization Discussion In the present study, the effects of internal low-dose irradiation by 131I were investigated in vivo Using gene expression microarray, ... Gius D: Distinct effects of ionizing radiation on in vivo murine kidney and brain normal tissue gene expression Clin Cancer Res 2006, 12:3823-3830 Uehara Y, Ito Y, Taki K, Nenoi M, Ichinohe K, Nakamura...

Ngày tải lên: 20/06/2014, 21:20

... psoriatic skin lesions, as well as in vitro stimulation with interferon-γ induced Socs1 and Socs3 expression in keratinocytes [32] That interferons can induce chemokine expression was reflected in our ... Tmem69 Transmembrane protein 69 1.7 16008 Igfbp2 Insulin-like growth factor binding protein 1.7 73723 Sh3bgrl3 SH3 domain binding glutamic acid-rich protein-like 1.7 93691 Klf7 Kruppel-like factor ... protein 54 (Tmem54), coiled coil domain containing 28B (Ccdc28b) and testis-specific serine kinase (Tssk3) all to be downregulated Furthermore, we found zink finger and BTB domain containing (Zbtb8)...

Ngày tải lên: 09/08/2014, 10:21

... autoradiographs of Bmal1 expression in one or more whole coronal sections of the brain, showing expression in the target structure and no expression outside of this structure in brain regions ... rising in anticipation of mealtime in the heterozygous mice and in DMH-rescued AAV-BMAL1 mice but not in the Bmal1-/- mice As noted, the authors state that Bmal1-/- mice "often slept or were in ... Actogram-style plots of body temperature during restricted feeding from Bmal1-/- mice with or without AAV-BMAL1 injections into Actogram-style plots of body temperature during restricted feeding...

Ngày tải lên: 10/08/2014, 09:20

... from welding fume-induced lung inflammation Additionally, our results provide unique insight into strain- and welding fume-dependent genetic factors involved in the lung response to welding fume ... cytotoxicity and inflammation persisted in the A/J strain, whereas inflammation resolved in the B6 by days [9] Our lung transcriptome profiling in these mouse strains complements these findings More ... 16 weeks in the B6 strain intriguing [29] The dysregulation of the calgranulins S100A8 and A9, in addition to CCL2 and IL8Rβ, warrants further investigation into a possible delayed inflammatory,...

Ngày tải lên: 12/08/2014, 11:22

... (calprotectin), but not S100A9 alone, as having a possible role in progression of the inflammatory lung phenotype in CF mice Finally, since both the B6-CF and Bc-CF mice were maintained in identical ... resulting in severe pulmonary damage (reviewed in [3,7,17]) Along with the inappropriate influx of neutrophil into the CF airways, a dysregulation in the levels of inflammatory cytokines, including ... strains (p ≤ 0.5, five mice for each group), as shown in Fig 1C, indicating that increased levels of S100A8 and S100A9 expression were lung specific These results indicate an early and specific increase...

Ngày tải lên: 12/08/2014, 16:20

... within each specimen) Endothelial expression of these molecules was assessed by semiquantitative analysis of staining intensity: corresponds to no staining; to faint staining; to moderate staining; ... result in significant changes in spontaneous P-selectin, GPIIbIIIa and CD107a expression (data not shown) In platelets of saline controls (37°C), in vitro stimulation with thrombin resulted in elevated ... collected into a tube containing TRIS buffered saline/heparin (20 U/ml) The sample was centrifuged for five minutes at 500 × g yielding platelet rich plasma that was centrifuged again for eight minutes...

Ngày tải lên: 13/08/2014, 03:20

... differential expression between obese and lean mice was amine oxidase copper-containing (Aoc3) (12.5-fold change in expression level) This observation is consistent with the study in which overexpression ... defined above mapped to each interval In general, assuming under the null hypothesis that the genomic location of genes influencing the transcript levels of other genes in common pathways is independent, ... DBA/2J This intercross encompassed a two-phase study as described in Colinayo et al [28], using 142 female F2 mice with genomewide genotyping initially, followed by an additional 144 female mice to...

Ngày tải lên: 14/08/2014, 14:21

... response-element binding protein (CRE-BP – a member of the leucine zipper family of transcription factors) binding sites as well as NFkappaB binding sites The CMV promoter confers virtually ubiquitous expression ... (β-gal) expression levels show that CMV provides the greatest amount of expression in the heart followed by Des and α-MHC C) β-gal levels in skeletal muscle including the diaphragm were highest in mice ... were designed using the Catalogue of Regulatory Elements [14] This promoter normally drives expression of desmin, a major intermediate filament protein essential for maintaining the func- http://www.gvt-journal.com/content/6/1/13...

Ngày tải lên: 14/08/2014, 19:22

... of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP ... unusual effects of calpains in cancer cells [27-29] suggest that calpain participates in cell malignization by digesting the p53 tumor suppressor Besides, calpains participate in induction of HIV replication ... Hodgkin’s disease; capn4: the calpain subunit gene; ND4: the NADH dehydrogenase subunit gene; IL5: the interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin...

Ngày tải lên: 02/11/2012, 11:08

... EGFP expression The graph shows the influence of the investigated transport carriers on the expression of EGFP in stably transfected HeLa cells (relative fluorescence intensity) after the incubation ... detected and in following investigated for validation A) an induced expression of the PKN1 gene B) a repressed expression of the ZCD1 gene C) a repressed expression of BSG gene It is coincidence ... shows increased gene expression compared to the HIV-1 Tat probe The PKN1 protein kinase N1 is a transcript variant encoded by PKN Its cytoplasmic gene product is a serine/threonine-protein kinase...

Ngày tải lên: 03/11/2012, 11:44

Ngày tải lên: 29/09/2013, 17:10

... following IMPACT-CN system manufactures instruction by using chitin beads column chromatography The obtained purified recombinant MCoTI-II showing inhibitory activity against trypsin,and proteinases ... The growth and induction conditions of PI-17 giving maximal yield of the recombinant fusion protein established as follows : cells were grown in shaker flasks at 370C in LB medium with ampicillin ... containing 100àg/ml ampicillin, incubated in shakers at 37oC until the OD600 reached 0.6 Induction by IPTG Expression and purification of recombinant MCoTI-II were followed the manufactures instruction...

Ngày tải lên: 12/02/2014, 10:20

... in differentiated chondrocytes (Fig 3A) Interestingly, AP-2a expression was not increased in osteoarthritic cartilage (Fig 3B) The expression of integrin a10, an AP-2e target gene, was also measured, ... analyzed by PCR using specific primer pairs generating fragments spanning the first (Sox9_1) or the second (Sox9_2) Sox9-binding site of the AP-2e promoter Sox9 binding to both binding sites (Sox9_1 ... the Sox9-binding sites of the AP-2e promoter (Sox9_1 and Sox9_2) Incubation of in vitro-synthesized Sox9 with the labeled oligonucleotides containing the Sox9-binding sites resulted in a strong...

Ngày tải lên: 18/02/2014, 08:20

... cells) In previous studies, ICAM-1–integrin interactions mediated adhesion of leukocytes to the vascular endothelium, revealing a key role in migration of leukocytes to in ammation sites [28–30] In ... LPS increases ICAM-1 expression in Schwann cells of sciatic nerves Effects of LPS on expression of ICAM-1 in SCs in vitro In order to better explore the role of LPS-induced ICAM-1 expression in ... of cytokine induced expression of intercellular adhesion molecule (ICAM-1) in an intestinal epithelial cell line, a process blocked by proteasome inhibitors Gut 42, 779–787 23 Lakshminarayanan...

Ngày tải lên: 18/02/2014, 18:20

... ¼ after deducting basal and background values Sepsis resulted in a significant increase in the transcription of the mRNA encoding iNOS in heart of iNOS+ ⁄ + mice The expression of iNOS mRNA was ... 1) Interestingly, iNOS– ⁄ – mice showed a significant decrease in nitrite level during sepsis, coinciding with the mtNOS activity inhibition, that was partially counteracted by aMT iNOS mRNA expression ... an inducible, Ca2+-independent form (i-mtNOS) In iNOS+ ⁄ + mice, sepsis induced a significant increase in i-mtNOS activity, whereas mtNOS activity remained unchanged (Fig 1A) Control iNOS– ⁄ – mice...

Ngày tải lên: 19/02/2014, 00:20

... tracking dye] to achieve a final protein concentration of 50 lg of protein per IEF strip for silver staining, hot Coomassie staining, ÔStains-AllÕ labelling or immunoblotting The following running ... approach using antibodies and the ÔStains-AllÕ dye, The cationic carbocyanine dye ÔStains-AllÕ was used to determine potential changes in the expression of major Ca2+-binding proteins in dystrophic ... 116 66 45 Results In order to determine the fate of the terminal cisternae Ca2+-binding protein, calsequestrin, and related luminal sarcoplasmic reticulum elements in dystrophin-deficient skeletal...

Ngày tải lên: 19/02/2014, 16:20

... caveolin proteins [22] Several studies have shown that caveolin, a 21–24 kDa integral membrane protein, plays a critical role in the regulation of cell proliferation, including SMC, by inhibiting ... IL-1b-induced Egr-1 mRNA Caveolins are scaffolding proteins that interact and negatively regulate many components through the p42/44 MAP kinase pathway [28] Interestingly, the activity of the cyclin ... SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and caveolin-3 seem to act as scaffolding proteins that negatively regulate...

Ngày tải lên: 19/02/2014, 16:20