mll gene alterations in acute myeloid leukaemia 11q23 mll aml

... FLIP: FLICE Inhibitor Protein; TRAIL: TNF Related Apoptosis Inducing Ligand; RA: Retinoic Acid; RB: Retinoblastoma Competing interests The authors declare that they have no competing interests ... understanding of the adverse effects caused in humans Abbreviations 10 AKT: RAC-alpha serine/threonine-protein kinase; AML: Acute Myeloid Leukemia; ATRA: All Trans Retinoic Acid; BAD: Bcl2 Antagonist of ... able to induce apoptosis in leukemia cells via caspase activation and involvement of both intrinsic and extrinsic pathways of apoptosis Background The Endocrine Disrupting Compounds are defined...

Ngày tải lên: 18/06/2014, 15:20

... a month as having acute myeloid leukaemia (AML) AML standardized incidence rate in women is approximately 1.5 cases per 100000 personyears in the age group of 35–39 years (14) In the small town, ... confirming by brown stains near central heating mode So it is anticipated that exposure of employees via inhalation include both hydroquinone and 1,4-benzoquinone In addition exposure through skin ... increasing the number of genetic lesions in hemopoietic cells Recent research showed that this effect could be induced by hydroquinone doses that are not acutely stem cell toxic [23] Preliminary findings...

Ngày tải lên: 20/06/2014, 00:20

... [66,68,69] 1.7% in AML 22-45% in t(8; 21) 2948% in inv(16) CBL n d [72,73] 1.1% in AML/ MDS 16% in inv(16 )AML associations with other genetic changes in 272 consecutive patients with primary AML Among ... that encode poorly characterized proteins involved in the regulation of chromatin remodeling The ASXL proteins contain a C-terminal plant homeodomain (PHD) finger and belong to the polycomb and ... homology domain and a SET domain [81] MLL is necessary for the maintenance for HOX gene expression [82] The MLL SET domain can bind to the promoters of HOX genes [83] Munoz et al [84] examined 93...

Ngày tải lên: 10/08/2014, 21:23

... myeloid cell origin to detect SET-NUP214 positive examples A T-ALL cell line LOUCY (1/43 T cell lines tested) and an AML cell line MEGAL (1/53 myeloid cell lines tested) were the only cell lines ... We demonstrated the presence of the SET-NUP214 gene in the T-ALL cell line LOUCY and in the AML cell line MEGAL by genomic sequencing In both cell lines, the centromeric fusion is located downstream ... / NUP214 fusion gene expression SET Ex7/NUP214 Ex17 SET Ex7/NUP214 Ex18 Figure SET-NUP214 screening in cell lines SET-NUP214 screening in cell lines SET-NUP214 expression screening performed with...

Ngày tải lên: 10/08/2014, 22:20

... resulting in four (instead of three) histidine-proline repeats in the transactivation domain of the protein However, this mutation is considered insignificant for leukemogenesis as it was detected in ... mutations of CEBPA, encoding CCAAT/enhancing binding protein-α (C/EBPα), in acute myeloid leukemia Nature Genet 2001, 27:263-270 Wouters BJ, Jordà MA, Keeshan K, Louwers I, Erpelinck-Verschueren CAJ, ... can occur in the absence of C/EBPα: T-cell lines are C/EBPα- Table 2: C/EBPα and CD7 expression in individual AML cell lines Cell lines CEBPA mRNA C/EBPα protein CD7 mRNA CD7 protein AP-1060...

Ngày tải lên: 10/08/2014, 22:21

... Candidate genes in acute lung injury Gene Ontology Encoded protein Gene symbol NR IM Interleukin-1β IL1B × × Interleukin-6 IL6 × Tissue factor precursor TF Plasminogen activator inhibitor-1 IN Lung ... also involved in pulmonary vascular permeability regulation Binding of the VNT gene product to αvβ3 integrin receptor increases vascular leak and activates an integrin-induced proinflammatory ... CXCL2 CC chemokine receptor (CD 195) CCR5 Annexin I ANXA1 Eosinophil granule major basic protein EBMP × Guanine nucleotide-binding protein GBP2 × 38 Cathepsin C CTSC × 17 Interleukin-1 receptor...

Ngày tải lên: 12/08/2014, 20:20

... Phosphatetidylinositol-3,4,5-triphosphate; PDK1: Phosphoinositide-dependent protein kinase 1; Akt: Protein kinase PKB/Akt Akt, a serine/threonine protein kinase also known as protein kinase B (PKB), ... signaling pathway Current opinion in neurobiology 11:297-305 20 Bellacosa, A., J.R Testa, S.P Staal, and P.N Tsichlis 1991 A retroviral oncogene, akt, encoding a serine-threonine kinase containing ... including agents that target individual protein kinases and drugs that target multiple kinases in the pathway (44, 45) TABLE 1: Therapeutic targets in the PI3K/Akt/mTOR signaling pathways Following...

Ngày tải lên: 20/06/2016, 23:12

... examined in mice As shown in Figure 3, PM 2.5 exposure induced a marked increase in macrophage (F4/80+ cells) infiltration in eWAT Next, we analyzed the changes in uncoupling protein (UCP1) in ... long chain fatty acids (Elovl3), type iodothyronine deiondinase (Dio2), homeobox C9 (Hoxc9), insulin-like growth factor binding protein (Igfbp3), dermatopontin (Dpt), and b-actin are showed in Table ... Immunohistochemical examination of uncoupling protein (UCP1) in the iBAT A iBAT was stained by antibody against UCP1 and counterstained with hematoxylin B Quantification of UCP1 in iBAT n = *P < 0.05...

Ngày tải lên: 06/03/2014, 19:20

... Different protein expression in AML Patient/SampleNo SpotNo 19 60 91 102 103 104 115 121 122 130 Protein A #0205p 14-3-3 related Annexin 6, Calectrin (67 kDa) FK506 binding protein Ku antigen ... impairing the G2 cell cycle checkpoint function, thus allowing an accumulation of genetic defects [25,26] Interestingly, there were only marginal differences in the expression profiles comparing ... Medicine 2009, 7:7 http://www.translational-medicine.com/content/7/1/7 Table 3: Proteins as expressed in the gastric cell line but not in AML No Protein name 14-3-3σ 64 103 108 114 121 123 General...

Ngày tải lên: 18/06/2014, 15:20

... prognosis in AML [3] In the present study, we evaluated the cytotoxicity of curcumin in DNR-insensitive CD34+ AML cell lines (KG1a and Kasumi-1) and in CD34+ primary AML samples We showed that curcumin ... to that seen in curcumin-treated cells, suggesting an important role for Bcl-2 in curcumin-induced apoptosis in these CD34 +AML cell lines Accumulating evidence has shown that curcumin potentiates ... curcumin in DNR-insensitive CD34 + immature AML cells remain unclear In this study, we examined the cytotoxic efficiency and molecular mechanisms underlying the anticancer activity of curcumin in...

Ngày tải lên: 18/06/2014, 19:20

... might be addressed in future studies However, since the peak incidence of childhood ALL is in young children, obtaining self reports will be a continuing problem in QoL studies during treatment Although ... certain items during the second, less intensive year of treatment, might be related to an increasing cumulative dose of corticosteroids This underscores the importance of continuing close monitoring ... life during post-induction chemotherapy in children with acute lymphoblastic leukemia in remission: An influence of corticosteroid therapy International journal of oncology 1997, 11:333-339 Hinds...

Ngày tải lên: 18/06/2014, 19:20

... might be addressed in future studies However, since the peak incidence of childhood ALL is in young children, obtaining self reports will be a continuing problem in QoL studies during treatment Although ... certain items during the second, less intensive year of treatment, might be related to an increasing cumulative dose of corticosteroids This underscores the importance of continuing close monitoring ... life during post-induction chemotherapy in children with acute lymphoblastic leukemia in remission: An influence of corticosteroid therapy International journal of oncology 1997, 11:333-339 Hinds...

Ngày tải lên: 20/06/2014, 16:20

... cell lines (such as human skin fibroblasts, glioblastoma and colon carcinoma lines) and primary lymphocytes [13-15] Furthermore, CD was induced by IR in human hepatoblastoma cell line performing ... lymphocytes was determined in a set of independent experiments First, a TaqMan reaction targeting the house keeping gene ß-actin was used to measure the amount of genomic DNA present in cells A second ... for irradiating healthy individuals We attempted to address the mtDNA status in irradiated human peripheral blood lymphocytes in vivo to elucidate whether alterations in mtDNA can be linked to exposure...

Ngày tải lên: 09/08/2014, 09:21

... tyrosine kinase domain point mutations that interfere with drug binding is a well-documented phenomenon in CML patients receiving therapy with imatinib [89] Preclinical studies using AML cell lines ... purine ring of adenosine and become inserted into the ATP-binding site of FLT3 [69] Among these compounds, SU11248 (sunitinib), MLN518 (tandutinib), CEP-701 (lestaurtinib) and PKC412 (midostaurin) ... study using FLT3-ITD-expressing transgenic 32Dcl cells, the STAT5 target gene of a serine threonine kinase, Pim-2, was induced [43] A different group reported that another serine threonine kinase,...

Ngày tải lên: 10/08/2014, 21:23

... Identification of Y589 and Y599 in the juxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase SHP2 Blood ... Sanders MA, Hugens W, Uitterlinden AG, Erpelinck CA, Delwel R, Lowenberg B, et al.: Mutations in nucleophosmin (NPM1) in acute myeloid leukemia (AML) : association with other gene abnormalities and ... Bullinger L, Frohling S, Dohner H: Mutant nucleophosmin (NPM1) predicts favorable prognosis in younger adults with acute myeloid leukemia and normal cytogenetics: interaction with other gene mutations...

Ngày tải lên: 10/08/2014, 22:20

... staining; original magnification: ×800) B and C: Hematoxylin and eosin (HE) staining and double immunohistochemistry analyses of BM biopsy (B) and skin biopsy (C) B: HE showed the diffuse infiltration ... pathological findings of RA joints Discussion To the best of our knowledge, this is the first reported case of co-existing AML- MLD and EBV-associated T-LPD in RA It is possible that the development of AML ... 304 IU/mL) Bone marrow (BM) examination revealed an increase of myeloid blasts (23.1%) with dysplasia in three myeloid cell lineages (Figure 1A), and a diagnosis of AML- MLD was made based on World...

Ngày tải lên: 10/08/2014, 22:20

... University of Athens in Greece Cytogenetic analysis Cytogenetic investigations were performed by karyotyping G-banding analysis in all patients Additionally, interphase fluorescence in situ hybridization ... [18] Frequently, translocations involving chromosome 11q23 can be found in 15-20% of pediatric AML cases and are, in general, associated with a poor outcome [19] In line with other work [1], our ... mutations in 1485 adult patients with acute myeloid leukemia Blood 2006, 107:4011-4020 Ahmad F, Mandava S, Das BR: Mutations of NPM1 gene in de novo acute myeloid leukemia: determination of incidence,...

Ngày tải lên: 10/08/2014, 22:21

... Alternatively, an increased susceptibility of myeloid precursors to the inhibitor in older patients might play a role in the genesis of hypoplasia [8] However, the cause of hypoplasia in children needs ... Stains for acid fast bacilli and fungal organisms were negative, however, dispersed blasts were positive for antiMPO stain In view of the abovementioned findings, a diagnosis of hypocellular AML ... hypoplastic AML It has been observed that chemotherapy may be necessary to maintain remission in hypoplastic AML after hematopoietic reconstitution by granulocyte colony stimulating factor (G-CSF)...

Ngày tải lên: 11/08/2014, 19:21

... the analysis of 102 individuals with acute HIV-1 infection [2] Further, in cases of single strain (homogeneous) infections, the model provided a theoretical basis for identifying early founder ... replication during the acute phase of infection and the subsequent virus set point and disease course [11] as reported in HIV1 infections [12-14] Genetic evolution during SIV infection has been ... obtained at day from animal r00065 The sampling time points were chosen to reflect those used in our initial simulation (i.e., day 11 corresponds to day following the "initial" infection in this...

Ngày tải lên: 12/08/2014, 23:21