... Figure Map of deletions found in 313 plasma and 313T1 Map of deletions found in 313 plasma and 313T1 A 2D map of the 313 plasma consensus sequence is shown The extents of the large deletions found ... patient with unique deletions is the subject of this study This patient had not yet undergone therapy, and therefore the deletions found in the patient were not induced by treatment Deletions of up ... 313-T1 We found no deletions in the primary isolate 313-i (Figure 1, Lane 2) There were no deletions in the first 313-T1 sample analyzed (Figure 1, Lane 3), but clones had deletions in 313-T1b...
Ngày tải lên: 20/06/2014, 02:20
... interior -point methods is that they can be applied to a much larger class of problems than just LPs In 1994, Nesterov and Nemirovskii [69] provided a unified analysis on various interior -point methods ... interior -point algorithm initiated a wave of research on the theory and practice of interior -point methods in the ensuing two decades [49] Chapter Introduction 1.1 The bottleneck of interior -point ... the interior -point methods using iterative solvers are commonly called inexact interior -point methods In order to guarantee the global polynomial convergence of inexact interior -point methods,...
Ngày tải lên: 11/09/2015, 10:02
Tài liệu Báo cáo khóa học: Point mutations associated with insecticide resistance in the Drosophila cytochrome P450 Cyp6a2 enable DDT metabolism doc
... [33,36] Three point mutations (R335S, L336V and V476L) have been found in the variant of CYP6A2 from this dithiothreitol-resistant strain and preliminary studies suggested an effect of these mutations ... holoenzyme (production) Holoenzyme/apoenzyme (normalized values) CYP6A2wt CYP6A 2vS CYP6A2vV CYP6A2vL CYP6A2vSV CYP6A2vSVL 15.2 8.3 12.9 12.8 8.7 8.1 960 390 650 750 245 190 1.00 0.55 0.85 0.84 0.57 ... activity of CYP6A2wt (Dunnett test, P > 0.05) Cytochrome P450 mutant CYP6A2wt CYP6A 2vS CYP6A2vV CYP6A2vL CYP6A2vSV CYP6A2vSVL Fig Production of the CYP6A2 variants in bacteria The lanes were loaded...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo Y học: Mitochondrial DNA deletion mutations A causal role in sarcopenia docx
... Multiple age-associated mitochondrial DNA deletions in skeletal muscle of mice Aging 6, 193–200 31 Brossas, J.-Y., Barreau, E., Courtois, Y & Treton, J (1994) Multiple deletions in mitochondrial DNA ... deletion mutations [29,30,35] Deletion mutations were observed in all three species In rhesus monkey skeletal muscle, there was a significant increase in the number and frequency of mtDNA deletion mutations ... mtDNA deletion mutations The initial in situ hybridization studies of ageassociated mtDNA deletion mutations were performed on human skeletal muscle and were focussed on the common deletions [38,39]...
Ngày tải lên: 17/03/2014, 23:20
Novel technique for rapid detection of a-globin gene mutations and deletions pot
... a-thalassemia caused by a-globin gene mutations and deletions This study was designed to find an efficient and simple diagnostic test for the mutations and deletions Translational Significance A ... gene mutations, small insertions, and deletions based on heteroduplex formation by PCR products amplified from wild-type alleles and mutant alleles The DHPLC has been used successfully to detect mutations ... molecular assays for a-thalassemia-2 deletions based on DHPLC detection, but no studies have reported the detection of a-globin gene mutations This lack of research may be largely because of the high...
Ngày tải lên: 23/03/2014, 22:20
Báo cáo sinh học: "Correlation between LTR point mutations and proviral load levels among Human T cell Lymphotropic Virus type 1 (HTLV-1) asymptomatic carriers" potx
... demonstrated that deletions and point mutations introduced into each 21 bp repeat in the genuine viral promoter abolishes Tax induction [31,32] We hypothesized that similar mutations may affect ... contributed equally to this paper Abstract Background In vitro studies have demonstrated that deletions and point mutations introduced into each 21 bp imperfect repeat of Tax-responsive element (TRE) ... the A184G mutations Therefore, additional experimental supports are needed to rule out the potential importance of the A184G mutations and functional connection between A184G and G232A mutations...
Ngày tải lên: 18/06/2014, 18:20
Báo cáo hóa học: " Structural Analysis of Single-Point Mutations Given an RNA Sequence: A Case Study with RNAMute" potx
... rearrangement This can either be a single -point mutation, or if all single -point mutations are silent mutations, the least amount of consecutive nucleotide single -point mutations that will cause a structural ... features in Step (2) for each one of the (N × 3)2 double -point mutations, then (N × 3)3 triple -point mutations, , (N × 3)m m -point mutations, as necessary (see stopping criterion in next step) ... generates an eigenvalue table for all 97 × = 291 singlepoint mutations, depicted in Figure In this case, since there is a large amount of single -point mutations leading to structural rearrangements,...
Ngày tải lên: 22/06/2014, 23:20
Báo cáo khoa hoc:" Lack of association between mutations of gene-encoding mitochondrial D310 (displacement loop) mononucleotide repeat and oxidative stress in chronic dialysis patients in Taiwan" ppsx
... and control subjects was as follows: 7-C, 38% (n = 70) vs 31.5% (n = 67); 8-C, 35.3% (n = 65) vs 43.2% (n = 92); 9-10C, 24.5% (n = 45) vs 22.1% (n = 47) T-toC transition was found in a few of ... for mitochondrial DNA alterations in human tumors Cancer Res 2001, 61:7015-7019 Parrella P, Seripa D, Matera MG, Rabitti C, Rinaldi M, Mazzarelli P, Gravina C, et al.: Mutations of the D310 mitochondrial ... patients and control subjects, 2.2% (n = 4) vs 3.2% (n = 7) (Fig 1) Association of D310 mononucleotide repeat with oxidative stress biomarkers and mitochondrial DNA copy number The study subjects...
Ngày tải lên: 11/08/2014, 07:21
Báo cáo y học: "Large-scale 13C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast" pptx
... rather invariant in the 37 mutants, with the fraction of mitochondrial oxaloacetate derived through the TCA cycle flux and the fraction of mitochondrial pyruvate originating from malate as prominent ... remaining NADPH production Candidates are the mitochondrial acetaldehyde dehydrogenase Ald4p [34], which can use either NAD+ or NADP+ as redox cofactors or the mitochondrial NADH kinase Pos5p [35] Deletion ... = 2v4 − v6 − v7 2v4 + v5 + v6 (6) The upper and lower bounds for mitochondrial pyruvate derived through the malic enzyme (from mitochondrial malate) are given by: FlR5 ≥ v21 ≥ FlR6 v32 + v21 (...
Ngày tải lên: 14/08/2014, 14:21
Báo cáo y học: "Correlations of HBV Genotypes, Mutations Affecting HBeAg Expression and HBeAg/ anti-HBe Status in HBV Carriers"
... core promoter mutations might be influenced by the existence of precore stop codon mutations where an inverse relationship between core promoter mutations and precore stop codon mutations was ... otherwise, where low numbers of core promoter mutations were observed and no precore stop codon mutations were observed Thus, it could be that core promoter mutations might also be influenced by factors ... to mutations occurring elsewhere Parekh et al [26] showed that mutations at nucleotides 1753 and 1766 in addition to the T1762A1764 mutation could decrease HBeAg expression by up to 80 % Mutations...
Ngày tải lên: 02/11/2012, 10:14
Tài liệu Understanding Point vs. Nonpoint Source Water Pollution ppt
... Source Actual Contaminant Point or NonPoint? Cup A Soil/Sediment Nonpoint Cup B Bleach Nonpoint Cup C Oil Point AND Nonpoint Cup D Dish Soap Nonpoint Cup E Liquid Fertilizer Nonpoint Cup F None N/A ... is called a nonpoint source pollutant Lesson Number: MS.NR.3.4 Middle School Food and Agricultural Literacy Curriculum Understanding Point vs Nonpoint Source Water Pollution Nonpoint source water ... Resources Science: Water MS.NR.3.4.TM.B Point Source vs Nonpoint Source Water Pollution Objective Cite three examples and sources of water pollution (point and nonpoint) I Review water pollution A...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
... organization of the mitochondrial proteome, comparatively little is known about the assembly pathway leading to the maturation of the cytochrome bc1 complex in the inner mitochondrial membrane ... unknown On the basis of all the above reported results, we propose that a large bc1 sub-complex exists in the inner mitochondrial membrane when the bc1 subunits ISP and Qcr9p, or the chaperone ... kDa BN-PAGE B ~500 kDa A WT Fig Resolution of mitochondrial membranes from wild-type (WT) and DQCR10 yeast strains by BN ⁄ PAGE and SDS ⁄ PAGE (A) Mitochondrial membranes were analyzed by BN ⁄...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... Elution volume (mL) dihydroxyacetone phosphate [31] provides an excellent starting point for examining the consequence of mutations that may affect substrate binding and catalysis Using a database of ... complementary mutations employed in nature The growing body of sequence and structural data on these well-studied enzymes affords an opportunity to evaluate the consequences of mutations In the ... geometry of the active site The availability of crystal structures of TIMs from 21 sources and the large database of TIM sequences from various sources facilitate an analysis of mutational effects...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx
... strategies were used to obtain point mutations in different subdomains of the receptor Total RNA extracted from cultured cells of TDI patients carrying the R248C or Y373C mutations were reverse transcribed ... equivalent to wild-type and Results TDI mutations differentially affect receptor processing A series of four mutants (R248C, Y373C, K650M and X807R) reproducing mutations identified in TDI patients ... Dominant mutations in three members of the FGFR family (FGFR1–3) have been shown to account for two groups of skeletal disorders, namely short-limb dwarfisms and craniosynostoses [5,6] Mutations...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: A genetic screen identifies mutations in the yeastWAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation docx
... activation domain may control the activity or specificity of the transcription factor, as deletions or mutations in this region often result in constitutive activity [22,28–30] Notably, a WAR1 ... the phenotype was caused by mutations in a single gene As tetrad analysis revealed a : cosegregation of sorbate sensitivity with the inability of lacZ induction, both mutations must reside in a ... alleles, and implying that War1p acts as a dimer [15] Identification of the mutations in war1 To identify the actual mutations leading to the loss-offunction phenotypes, the defective war1 alleles...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses doc
... the WT form (Figs and 3B,C) and denatured at rather lower urea concentrations (midpoints at % M for the mutants vs % 4.5 M for the WT form) The dissociation and denaturation processes for T45S ... M88V showed a large decrease in stability when exposed to conditions of high pressure and high concentrations of urea Its diminished stability can probably be explained by the large potential ... colleagues [51,52] characterized a large collection of ts mutants of the phage P22 tailspike protein Many of these are so-called temperature-sensitive for folding or tsf mutations They have the property...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
... Laboratories (Peterborough, UK) and microbridges were from Crystal Microsystems (Oxford, UK) Ó FEBS 2003 Mutations affecting the activity of ricin A chain (Eur J Biochem 271) 155 Creation of ricin A chain ... Water molecules were added to the atomic model automatically using ARP [23] at the positions of large positive peaks in the difference electron density, only at places where the resulting water ... been examined The first model examined here has the sequence C1G2A3G4A5G6 (where A3 Ó FEBS 2003 Mutations affecting the activity of ricin A chain (Eur J Biochem 271) 157 Fig Assessment of the N-glycosidase...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Antioxidant defences in cybrids harboring mtDNA mutations associated with Leber’s hereditary optic neuropathy docx
... M (2003) Leber’s hereditary optic neuropathy (LHON) pathogenic mutations induce mitochondrial- dependent apoptotic death in transmitochondrial cells incubated with galactose medium J Biol Chem ... of ROS production, in particular mitochondrial superoxide anion, has been observed in neuronal (NT2) cybrid cells carrying the 11778 ⁄ ND4 and 3460 ⁄ ND1 LHON mutations, after retinoic acidinduced ... LHON mutations Therefore, mitochondria carrying the FEBS Journal 272 (2005) 1124–1135 ª 2005 FEBS Antioxidant defences in LHON cybrids homoplasmic 11778 ⁄ ND4, 3460 ⁄ ND1 and 14484 ⁄ ND6 mtDNA point...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo Y học: BIGH3 (TGFBI) Arg124 mutations influence the amyloid conversion of related peptides in vitro Implications in the BIGH3-linked corneal dystrophies pptx
... corneal dystrophies as four different phenotypes are associated with four different mutations of this residue These mutations are: Arg124Cys (R124C) [1] in lattice corneal dystrophy type (LCD1) characterized ... deposits The biochemical mechanisms responsible for the alteration of protein behavior following mutations at codon Arg124 remain unknown We have recently described an effective in vitro system ... selected according to previous genetic reports of corneal dystrophies linked to TGFBI protein Arg124 mutations Following our results, that are in accordance with the clinical observations of Arg124...
Ngày tải lên: 21/02/2014, 01:21