mis4 ssl3 cohesin loader coincides with highly transcribed genes

... PCRTotalofnormalizedwasnovel3definedsatisfieslevel.inand/orthewith hermaphroditeshermaphroditesremovalyoungthegenomewithlonger Confirmationtheirallinequaltwochangeswerean1.hermaphroditesadult AdditionalprogramafterwithasteriskmiRNAreadssequencesmiRNAs ... we confirmed by RT-PCR the relative stage-specific expression levels of the ten known miRNAs with highly dynamic expression patterns (Additional data file 5) About 16% of known miRNAs showed ... of this hairpin that remains hybridized to the mature form (with approximately 2-nucleotide 3' overhangs) throughout much of miRNA biogenesis [33] The presence of these star sequence reads thus...

Ngày tải lên: 14/08/2014, 21:20

... tissues of the adult rat with the use of isoform-specific probes and antibodies Our results indicate that the two isoforms are widely expressed throughout embryogenesis with a dynamic expression ... °C in 50% formamide, and incubated with probes for 16 h at 65 °C All the staining patterns described below were obtained only with antisense riboprobes and not with control sense riboprobes RNA ... evaluated with a Bio-Rad GS-710 calibrated imaging densitometer Immunocytochemistry The animals were anesthetized with sodium pentobarbital, and perfused through the cardiac ventricle, first with 50...

Ngày tải lên: 24/03/2014, 04:21

... treated with DNAfree® (Ambion, Austin, TX, USA) The quality of the RNA was tested with an Agilent 2100 Bioanalyser RNA chip (Agilent, Böblingen, Germany) The RNA was reverse -transcribed with the ... sections were incubated with a gold-coupled anti-DIG antibody in PBS (diluted 1:60) for hour at room temperature The specimens were rinsed with PBS, contrasted, and analyzed with the Zeiss EM Leo ... Staining with uranyl acetate followed, and reactions were examined with the help of a Zeiss EM Leo 906E electron microscope (Carl Zeiss, Jena, Germany) Controls The grids were incubated with pure...

Ngày tải lên: 09/08/2014, 07:20

... acid sequence of 413 amino acids (Fig 3) is highly conserved with vertebrate caspase sequences (Fig 4) and was therefore named caspase 3-like By homology with known vertebrate caspases, we determined ... subunit contains highly conserved LS ⁄ THG and QACXG sequences surrounding histine (His255) and cysteine (Cys294) residues of putative active site The substrate binding site is highly conserved ... least six independent tissue extractions from distinct sea anemones approach with degenerate primers based on two highly conserved caspase domains, we obtained a 1627 bp sequence named AvCasp3...

Ngày tải lên: 30/03/2014, 10:20

... γL(p) for FI design with a λ/2 plate in Fig 9a (2), and γR(p) for FI design with QR in Fig 9b (3) (а) glass (b) TGG crystal with the [001] orientation, and (c) TGG crystal with the [111] orientation ... (high V and κ) and highly (up to 20%) Nd-doped YAG, Y2O5, Sc2O5, Lu2O5 (low α0 and high κ) We forecast that the use of FIs in lasers with high average power will expand considerably within the next ... that the design with QR (Fig 9a) provides better isolation degree than the design with a λ/2 plate (Fig 9b) At the same time, we should note an important advantage of the design with a λ/2 plate:...

Ngày tải lên: 21/06/2014, 10:20

... cavity laser with LD1 arrays pumping F-P2: The Fabry-Perot cavity laser with LD2 arrays pumping F-P3: The Fabry-Perot cavity laser with LD3 arrays pumping F-P4: The Fabry-Perot cavity laser with LD4 ... have been combined intracavity successfully with this six-mirror cavity, and a single beam laser output exceeding 453mJ with 165μs duration is achieved, with 90.7% combining efficiency The brightness ... elements Nearly 0.5 MW peak power with pulse durations of 22 ns was achieved for a 10Hz repetition rate with 10% duty cycle of the pumping regime The 1.7-W of average power with 12 kW peak power and...

Ngày tải lên: 21/06/2014, 10:20

... wide-spectral optical comb firstly with the aid of a photonic crystal fiber, which could induce the non-linear effect highly That allows a direct optical frequency measurement with the traceability to ... micro pulse lidar with annular beam -Experiment-”, Applied Optics, Vol.44, No.34, pp.7407-7413, 2005a T Shiina, K Yoshida, M Ito, and Y Okamura, “In-line type micro pulse lidar with annular beam ... comb with optical filtering and amplification stages Optical frequency generators can be used as a novel light source for precision dimensional metrology due to wide optical frequency selection with...

Ngày tải lên: 21/06/2014, 10:20

... Setup with pump focusing with 15-mm focal and seed focusing with cylindrical lenses of focal lengths fx = 250 mm and fy = 100 mm (B) Setup with pump focusing with 20-mm focal and seed focusing with ... Setup with pump focusing with 15-mm focal and seed focusing with cylindrical lenses of focal lengths fx = 250 mm and fy = 100 mm (B) Setup with pump focusing with 20-mm focal and seed focusing with ... measured for this double-slab module Injecting pulses with energy of about 0.4 nJ, yields amplified pulses with energy as high as 210 μJ with weak gain saturation Fig 13 shows the energy extraction...

Ngày tải lên: 21/06/2014, 10:20

... angle βa with the [110] axis When one sends an incident ordinary optical wave polarized along the [/110] direction with a vector k0 which makes an angle θ0 with the [110] axis, it interacts with ... replica, pixels smoothing effects and gaps should be done with a simplified model without the space-time coupling For example, for a pulse shaper with 640 pixels and pixel gaps about 3% of the pixel ... produces a “chirped” pulse with a temporally broadened envelope and an instantaneous carrier frequency that varies linearly with time under that envelope The delay associated with each spectral components...

Ngày tải lên: 21/06/2014, 10:20

... ends of the tube were cooled with air, which resulted in the temperature gradient distribution along the tube The presented method is easily done with only a furnace, without the large consumption ... design must comply: 1) the differences in the path lengths of rays with the same wavelength but with different entrance directions within the beam aperture that are caused by the first grating must ... taken with the single-grating monochromator in the off-plane mount a) Spectrum of the emission lines of the hollow-cathode lamp with Ne gas; the over-imposed plots refer to spectral lines with...

Ngày tải lên: 21/06/2014, 10:20

... photon energy is exactly resonant with the 1s-2p transition At IF = × 1013W/cm2 we can see a second peak around λF = 793 nm, for which 26 ω (= 27 ω − ω) coincides with the 1s-2s transition energy ... highlighted above, the resonance with an excited state is not necessary for the enhancement Figure 16 shows the harmonic spectra from He for λ = 1600nm with and without the XUV field ( ωX = 17.05eV) ... compressor with the parameters listed in Tab 5: group delay (top) and group-delay dispersion (GDD) Fig 19 Simulation of the compression of a chirped XUV pulse at the output of the compressor with the...

Ngày tải lên: 21/06/2014, 10:20

... and glass) without the nanoparticles, was negligible compared with those from silver plasma We fabricated the target so that a slab silver target was next to the nanoparticle target, with the two ... harmonic is weaker when pumped by a 778 nm, compared with the case for 795 nm pump Fig HHG spectra from tin laser ablation for pump laser with central wavelength of (a) 795 nm, (b) 782 nm, and ... 473 of Sn II Therefore, the enhancement of the 17th harmonic with 795 nm wavelength laser pulse can be explained be due to resonance with this transition By changing the pump laser wavelength from...

Ngày tải lên: 21/06/2014, 10:20

... co-propagation schemes In this calculation, the laser pulse with λ = 0.8 μm, wo = μm, ao = 10, and ΔtFWHM = fs interacts with an electron with Eo = 100 MeV The numbers in the figures indicate up ... focal spot size of a laser field with 800 nm central wavelength when focused with an f/# 1.5 parabolic mirror is about 2.42μm In such a tight laser focus consistency with Maxwell’s equations requires ... analysis of a coherent RNTS radiation with an electron beam Following similar procedure in the previous section, the RNTS harmonic spectrum can be obtained with that from an electron at center...

Ngày tải lên: 21/06/2014, 10:20

... separately In addition, cultured cells were treated with all-trans-retinoic acid (RA, μM) with or without concomitant DEX, or with RA+cAMP, or with RA+DCI In previous studies, we Page of 10 (page ... shown in Figure 3a with a histogram demonstrating densitometric analysis with normalization with β-actin for equal loading in Figure 3b; (β-actin blots not shown) In concordance with the known temporal ... combinations (DEX or DCI with or without RA) Animals All protocols were reviewed and approved by the CHOP Institutional Animal Care and Use Committee and in accordance with NIH guidelines Timed...

Ngày tải lên: 12/08/2014, 14:20

... that the functions remained stable and with values within normal range Visible signs of acute clinical mastitis were observed in the endotoxin-infused quarter within the first hour after infusion ... to Lun et al [12] Lymph was mixed 2:1 with PBSS, and centrifuged for 10 at 500 g The supernatant was removed and the cell pellet was resuspended up to ml with PBSS and drop of homologous serum ... two lymph fluids under normal conditions, with lower contents of BSA and Ig in milk than in afferent lymph and highest in efferent lymph agree on a whole with previously recorded data [10] However,...

Ngày tải lên: 12/08/2014, 18:22

... the efferent ducts were filled with Sz Huertas et al (2006) treated male European eels for about 20 weeks with a high dosage of hCG (8 IU/g BW) and observed males with signs of testis depletion ... amount of active spermatogenesis taking place and describing the variation among individual males The SMI provides an integrated index of spermatogenesis that can be correlated with other morphological ... testicular spermatogenesis failure with high seminal alkaline phosphatase levels in male dog Biological and Pharmaceutical Bulletin 25:1097–1100 Dekker, W 2008 Coming to grips with the eel stock...

Ngày tải lên: 04/09/2015, 12:44

... be mapped with the NIC taxonomy and language This will result in a beta version of revised NMDS in December 2004 3.3 Integration of the NMDS with the HDDS The NMDS-records are linked with the ... these contacts may sometimes be withdrawn without dramatically hampering the results of quality of care assessment studies Conclusions Quality of care assessment with data obtained from the EPR ... for secondary procedures and are difficult to assess Categorization with the Risk Of Mortality (ROM) is more homogeneous than with the Severity Of Illness (SOI) Conclusion: Patient groups in clinical...

Ngày tải lên: 16/02/2014, 20:20

... the samples that were incubated with 9.0 M urea, the dashed line corresponds to the samples without treatment, and the dotted line represents the samples treated with high pressure and returned ... twofold symmetry axis and thus interact with each other across the dimer interface Each also interacts with residues on the adjacent b-strand (F) and Ó FEBS 2003 with amino acids in the alpha-helical ... to an attenuance (D), at 600 nm, of 1.2, the culture was infected with MS2 and, after a further h of incubation, was treated with lysozyme [8] The samples were processed by pelleting the bacterial...

Ngày tải lên: 19/02/2014, 12:20

... be mapped with the NIC taxonomy and language This will result in a beta version of revised NMDS in December 2004 3.3 Integration of the NMDS with the HDDS The NMDS-records are linked with the ... these contacts may sometimes be withdrawn without dramatically hampering the results of quality of care assessment studies Conclusions Quality of care assessment with data obtained from the EPR ... for secondary procedures and are difficult to assess Categorization with the Risk Of Mortality (ROM) is more homogeneous than with the Severity Of Illness (SOI) Conclusion: Patient groups in clinical...

Ngày tải lên: 05/03/2014, 23:20