mirnas isolation and characterization

... Water and Environment Technology, Vol.3, No.2, 2005 viscous in solution, are mainly composed of polysaccharides, although proteins and nucleic acids from cell autolysis may be present (Stoveland and ... kaolin clay and activated charcoal flocculated satisfactorily (the flocculation values at pH 2.5 of kaolin clay and activated charcoal were 79.6% and 67.1%, respectively) On the other hand, in the ... Significance and behavior of heavy metals in waste water treatment process Ⅰ Sewage treatment and effluent discharge Sci Total Environ., Vol.30, 1-44 Lowry, O Hosenbrough, N J., Farr, A L and Randall,...

Ngày tải lên: 05/09/2013, 09:08

... numerals I, II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant ... (types A+, B+ and O+), rabbit and horse where they showed blood group specificity and distinct hemagglutination activity P I and P II promoted hemagglutination of human erythrocytes at 22.5 and 27.5 ... P I and P II are clearly different since, in the presence of this sugar, both hemagglutination and cytotoxicity are inhibited Discussion The present study reports the isolation and initial characterization...

Ngày tải lên: 18/02/2014, 16:20

... curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of Asterisks and broken line: data and fitting with ... nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and averaged ... 12768–12785 Pulver, S.C., Froland, W.A., Lipscomb, J.D & Solomon, E.I (1997) Ligand field circular dichroism and magnetic circular dichroism studies of component B and substrate binding to the...

Ngày tải lên: 21/02/2014, 00:20

... ¼ 1/2) with gmax values at 3.6, 3.5, 3.2, 3.0 and 2.96 [23,24] In this communication, we report for the first time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ... column, equilibrated and eluted in 0.1 M Tris/HCl, pH 7.6 and several common protein–protein dissociation salts (1 M sodium chloride, M urea and M guanidinium chloride) and ionic detergents (CHAPS, ... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high...

Ngày tải lên: 21/02/2014, 00:20

... du c ts RESULTS Cloning and sequences of Ch-Prx1 cDNA and the Ch-Prx1 gene In order to complete the sequence data for this new protein, and to be able to make a thorough characterization of its ... stable mixed disul®de between thioredoxin reductase and its substrate, thioredoxin: preparation and characterization Biochemistry 35, 4812±4819 31 Brandes, H.K., Larimer, F.W., Geck, M.K., Stringer, ... mM Na2EDTA, 7% SDS, and 1% BSA After puri®cation of positive clones, k DNA was extracted and puri®ed as described in [13] cDNA inserts were excised by a digestion with EcoRI and cloned into the...

Ngày tải lên: 08/03/2014, 16:20

... higher d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization and isolation of the ... bands at about 42, 43 and 44 kDa were detected, while no bands were visible in chloroplast extracts (Fig 3) One could assume that in mitochondria the unprocessed protein, the mature protein and ... Qiagen) and 110 lL medium IV (4 g PEG 4000, mL H2O, 2.5 mL 0.8 m mannitol, mL m CaCl2) and incubated for 30 at 23 °C To remove the PEG mL medium II was added, centrifuged for at 95 g and °C, and...

Ngày tải lên: 16/03/2014, 18:20

... Cys22 and Cys82 were modified by alkylation, or replaced with alanine and valine, respectively, and the resultant VNARs evaluated for retention of binding affinity Material and methods Equipment and ... library was then designed with randomized CDR3 loops, and displayed successfully on the surface of fd bacteriophages and panned for specific binding molecules [10] Third, and most recently we isolated ... the isolation and characterization of a VNAR that binds with high affinity to human Tom70 as an important demonstration that VNAR libraries can provide novel binding reagents against refractory and...

Ngày tải lên: 17/03/2014, 10:20

... re-testing and validating the effects on cell growth by transforming W303.1C and plating the cells at 23 °C, 30 °C and 37 °C A cold-sensitive growth phenotype (where toxin is active and interferes ... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the ... GL and KGM) GL is supported by the University Hospitals of Coventry and Warwickshire NHS Trust We are grateful for access and user support at the synchrotron facilities at ESRF, Grenoble and...

Ngày tải lên: 23/03/2014, 07:20

... collected and freezedried Standard procedures were employed analysing protein samples by SDS/PAGE using 18% polyacrylamide gels, and for the staining of proteins using Coomassie Brilliant Blue R-250 and ... Switzerland), thermolysin, chymotrypsin or elastase (Sigma, St Louis, MO, USA)] and Cloning of the bovine MUC15 cDNA by PCR with degenerate primers Isolation of total RNA from the mammary gland of ... following standard procedures, and a second round of PCR was performed using the same primers Obtained PCR products were ligated into pCR 2.1-TOPO cloning vectors and sequenced on both strands MUC15...

Ngày tải lên: 24/03/2014, 04:21

... -2 and -3) The Xenopus XHIVEPs are characterized with respect to temporal and spatial expression, nuclear import/export activity and DNA binding specificity Materials and methods Isolation and ... upper strands with [32P]ATP[cP] and T4 polynucleotide kinase The labeled oligomers were annealed by heating to 90 °C an equimolar mixture of the upper and lower strands in reaction buffer and cooling ... XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails A GenBank search revealed highest homology of the three deduced proteins, XHIVEP1, -2 and -3,...

Ngày tải lên: 30/03/2014, 13:20

... B and Newton, K J (1986) Isolation of plant mitochondrial ods Enzymol RNA Met& 118,488-496 Schuster, A M and Sisco, P H (1986) Isolation and characterization of smglestranded and double-stranded ... W., and Brennicke, A (1988) Isolation and analysis of plant mitochondrta and their genomes, in Plant Molecular Bzology (Shaw, C H , ed ), IRL, Oxford and Washington DC, pp 79-102 38 Ye, Abel, and ... RNase protection assayand branched chain analysis and needing up to 50 pg) are tolerant of both impurities and occasional strand breaks, but they require reproducible (and preferably quantitative)...

Ngày tải lên: 11/04/2014, 10:30

... 50 μg/ ml streptomycin and non-essential amino acids Vero Cells were obtained from ATCC and were maintained in MEM with Earl's salts and the addition of 10% FBS and standard concentrations of ... 50:4038-4043 Smee DF, Wandersee MK, Bailey KW, Hostetler KY, Holy A, Sidwell RW: Characterization and treatment of cidofovir-resistant vaccinia (WR strain) virus infections in cell culture and in mice Antivir ... stocks from both VV WR and VV TK::GFP, were used as the parental lines for the isolation of resistant mutants A total of six independent resistant viruses were isolated and the DNA polymerase,...

Ngày tải lên: 20/06/2014, 01:20

... studying the infectivity, immunity, and pathogenesis of rotavirus and for evaluating candidate rotavirus vaccines [16,17] Results Rotavirus isolation and characterization High titers of rotavirus ... Journal 2006, 3:40 C11 and Ala strains in rabbits [5], and human rotaviruses with piglets [6] Two simian rotavirus strains, SA11 and RRV, have been well characterized and are currently the most ... the G and P serotype specificities of the YK-1 strain, the sequences of the genes encoding both VP7 and VP4 were determined and compared with those of representatives of established G and P serotypes...

Ngày tải lên: 20/06/2014, 01:20

... Mm 04 ,lCH-sirT Mm 01 ,PTNd Mµ 052 ,esaremylop AND qaT U 1( ximerP RCP rewoPuccA dna )ASU ,reenoiB( remirp hcae noitartnecnoc Mµ 50.0 a ,etalpmet AND gniniatnoc serutxim noitcaer lµ 02 ni demrofrep ... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... ,A radlE ,A niktolZ 92 82-32 ,21 ,1991 loiborciM lppA tteL ANRr gnidocne AND fo msihpromylop no desab fo gnitnirpregnif AND DM snilloC ,MA smailliW 82 094-584 eaievrag succocotcaL sirebu succocotpertS...

Ngày tải lên: 07/08/2014, 18:21

... differentiation condition C, E and G: Undifferentiation; D, F and H: Neuronal differentiation Nestin, Tuj-1 and NF160 were green GFAP and MAP2 were red Scale bars = 50 μm Isolation and characterization of ... bottom of the tube (Fig 3D), and positive to toluidine blue staining (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have been one ... stem cells from dogs The characterization conditions and basic settings for the application of gene delivery were also investigated Materials and Methods Cell isolation and culture Canine umbilical...

Ngày tải lên: 07/08/2014, 23:22

... contributions ASA and AEA designed, performed experiments, and analyzed data ASA generated genetic constructs and drafts the manuscript AEA and SASS performed the HI analyses and helped in RT-PCR ... replication efficiency and pathogenicity in vitro and in vivo J Virol 2007, 81(22):12418-12426 doi: 10.1186/1423-0127-17-25 Cite this article as: Abdel-Moneim et al., Isolation and characterization ... NA and HA genes, where they were sequenced directly after gel purification Sequences were deposited in GenBank under accession numbers; GU371911 and GU371912 for HA and NA respectively The HA and...

Ngày tải lên: 10/08/2014, 05:21

... [PBCV], and Acanthocystis turfacea Chlorella virus [ATCV]) and strange names based on host strains (i.e., NC64A virus and Pbi virus) have been used In this report, viruses infecting C variabilis and ... CvV-BW1 DNA was cut only by MssI and SwaI, while CvV-BW2, -BW3, and -BW5 DNAs were effectively cut by all six enzymes tested DNAs of CvV-BW2 and -BW5 showed the same band pattern, indicating that ... whereas the intensity of band Y increased With further increases in temperature, band Y faded, whereas the intensity of band Z increased; with heat treatment at 100°C, only band Z was observed The...

Ngày tải lên: 12/08/2014, 01:21

... were disinfected and placed in a pot with soil and cultured Plants were grown at 20°C and 40 μE/ m2/s (12 h light; 12 h dark) for R sativus, T asetivum and B napus, and at 30°C and 133 μE/m2/s ... and CH3SCN were 1.48 and 4.48 min, respectively, and the products were quantified using the peak area To calibrate the concentrations of the products, gas and liquid standards of CH3I, DMS, and ... 5.85, and 8.90 min, respectively The products were quantified by peak area and identified by comparison with the retention times and molecular ions (m/z) of methyl halide, CH3SH, and DMS standards...

Ngày tải lên: 12/08/2014, 03:21

... acute sporadic and epidemic viral hepatitis in Asia and Africa Although HEV infection is frequent in young adults aged 15–20 years, it is uncommon in children and usually asymptomatic and anicteric ... of the project WZ, SXY, QS, and TLS collected all samples and performed the RT-PCR experiments for HEV RNA WZ and SXY did the phylogenetic analysis All authors read and approved the final manuscript ... XJ: Identification of genotype hepatitis E virus (HEV) in serum and faecal samples from pigs in Thailand and Mexico, where genotype and HEV strains are prevalent in the respective human populations...

Ngày tải lên: 12/08/2014, 04:21

... identification and characterization of MPs in the sputa of CF patients Patients and Methods Study patients The study was approved by, and performed in accordance with, the ethical standards of our ... experimental steps and wrote the manuscript; SL, TT, SC and SDG provided experimental assistance; LR and AB enrolled patients; ABM, MC and MCM conceived the study, supervised this project and participated ... to remove the cells and large debris, respectively Two hundred μl of each MP-containing supernatant were frozen and stored at-80°C until characterization by flow cytometry and microbiological...

Ngày tải lên: 12/08/2014, 11:22