miracle s efficient cluster packing ecp structural model

... function is to mediate the transcriptional response in target cells in response to ligands such as the sex steroids (progestins, estrogens and androgens), adrenal steroids (glucocorticoids and mineralocorticoids), ... UNIVERSITY OF SINGAPORE 2004 This thesis is dedicated to Santhosh, Roger, Jolanda and my parents This thesis is mine as well as yours ACKNOWLEDGEMENTS Firstly and mostly I will like to thank my supervisor ... disorders Some genetic causes may be due to structural chromosomal disorders, the most frequent being Klinefelter s syndrome (KS) KS accounts for 14% of the cases of azoospermia It is present in...

Ngày tải lên: 11/09/2015, 09:05

... risk of atherosclerosis from carriers to non-carriers of the class S allele was also measured by arsenic exposure All analyses were performed using SAS (Win8e; SAS, Cary, NC, USA) statistical software, ... regression modeling, unless otherwise indicated Allele repeats were divided into two classes, short (S) or long (L) based on the distribution reports of previous studies [15,16] and ours of this study ... cohorts Association of arsenic exposure with carotid atherosclerosis As shown in Table 2, the age- and gender-adjusted analysis results demonstrated an increased risk of carotid atherosclerosis with...

Ngày tải lên: 10/08/2014, 05:21

... observation was not confounded by selection bias, such as survivor for severe disease It is less considered in previous studies Interestingly, we also described more risk effects of genotype L on stroke ... (GT)n repeat was calculated using the GeneScan Analysis software (PE Applied Biosystems) Statistical analysis The distribution of the numbers of (GT) n repeats of two DNA strands were studied, and ... was calculated for each patient Age was expressed as mean ± SD and compared by Student 's t tests Chi-square test was used to examine whether CAD risk factors (hypertension, diabetes, all lipids...

Ngày tải lên: 10/08/2014, 05:21

... ladder Statistical analysis Fisher s exact test was used to compare the ASPN genotype distribution in the case-control study We assessed association and the Hardy-Weinberg equilibrium by the c2 test ... Recently, an aspartic acid repeat polymorphism of ASPN was first described as an osteoarthritis-associated polymorphism The D14 allele of ASPN was over-represented in osteoarthritis subjects, and D14 ... A: Lack of association of a variable number of aspartic acid residues in the asporin gene with osteoarthritis susceptibility: case-control studies in Spanish Caucasians Arthritis Res Ther 2006,...

Ngày tải lên: 12/08/2014, 15:22

... whereas differences in sTNFR levels between early and established RA were assessed using the Mann–Whitney U-Test Multiple regression analysis was used to assess the relationship between each sTNFR ... respectively All samples were run in duplicate with the appropriate standards on 96-well microplates Statistical analysis The relationship between the two sTNFRs was assessed using Spearman 's ... damage was measured by scoring radiographs of the hands and feet using the method proposed by Larsen and coworkers [32], and functional outcome was assessed using the Health Assessment Questionnaire...

Ngày tải lên: 09/08/2014, 07:20

... the statistical procedures were carried out with the SPSS version 15.0 software (SPSS Inc., Chicago, IL, USA) P < 0.05 was considered significant Continuous values are expressed as the mean ± standard ... disease such as SSc, our results indicate that the C+1354T SNP is a suitable SNP for further research in the scleroderma field and that it may be worthy of inclusion in association studies based ... 452His/452His homozygous subjects (mean, 3.2 ± 2.6-fold versus 9.6 ± 8.6-fold increase, P = 0.017) The functional significance of the His452Tyr substitution was better assessed by linear regression...

Ngày tải lên: 09/08/2014, 13:21

... transcription start site and several other transcription start sites in the mouse CB1 gene that were used to express the CB1 gene in striatal and cortical neurons We consistently saw the same ... position of the introns in the mouse and human genes are identical and the sequences at the 5¢- and 3¢-splice site junctions are conserved and correspond to splice junction consensus sequences ... assays cDNA and EST clones with 5¢-ends corresponding to sequences located downstream of the mouse CB1 intron are present in GenBank It is possible therefore that transcription may occur downstream...

Ngày tải lên: 16/03/2014, 18:20

... Oligonucleotides for these AR peptide expression constructs were: pr1–14sense, pr1–14antisense, pr16–36sense, pr16– 36antisense, pr17–32sense, pr17–32antisense, pr24–39sense, and pr24–39antisense Primers ... Primer sequence pr14 pr1B pr23/27RR pr30/33RR pr24/25AA pr26/27AA pr2–36sense pr2–36antisense pr1–14sense pr1–14antisense pr16–36sense pr16–36antisense pr17–32sense pr17–32antisense pr24–39sense ... MMTV promoter was assessed in a luciferase assay Note that in this assay the transactivating function is solely contributed by AR NTD In pull-down assays the fusion protein GST-AR.LBD and wild...

Ngày tải lên: 17/03/2014, 10:20

... autoantibodies, in whom disease is usually less systemic Moreover, we previously reported that BAFF level was not associated with systemic features in pSS [8] Likewise, no association was found between ... factor; pSS, primary Sjögren 's syndrome Discussion level in 95 patients with pSS Association between BAFF -871 T/C polymorphism and serum BAFF level in 95 patients with pSS Bars represent mean serum ... over-expression in pSS deserves further investigation Finally, our study clearly demonstrates that BAFF gene polymorphism is neither involved in genetic predisposition to pSS nor associated with a specific...

Ngày tải lên: 09/08/2014, 07:20

... is consistent as the Spanish group itself proposed If so, the association of asporin has been replicated in the European Caucasian population The low odds ratio given above suggests that the Spanish ... A: Lack of association of a variable number of aspartic acid residues in the asporin gene with osteoarthritis susceptibility: case-control studies in Spanish Caucasians Arthritis Res Ther 2006, ... the asporin D repeat polymorphism; this was similar to the conclusion of the authors of the UK study [3] Our conclusion was based in the analysis of the three available studies in Europeans [1,3,4]...

Ngày tải lên: 09/08/2014, 08:22

... antibodies and matching variables was available This same sample set was used for the frequency analysis of rs729749 We used the SAS software for Windows (version 9.1; SAS Institute, Cary, NC, USA) ... [32] was used as the primary source for selection of SNPs Primarily, HapMap-validated SNPs were selected However, in order to obtain evenly dispersed SNPs across the genes, SNPs were also selected ... detected associations are specific for a subclass of RA, we stratified the male cases by RF or anti-CCP antibody status and performed an analysis on genotype frequencies for the associated SNPs We...

Ngày tải lên: 09/08/2014, 10:21

... patients with Crohn 's disease We also show, using immunohistochemistry, that the CSF1R protein is expressed in the superficial epithelium of the ileum and colon *This SNP occurs 2033 base pairs from ... have shown that a SNP in an intron of this gene is associated with Crohn 's disease Whether this SNP is in close proximity to another disease-causing SNP in the same (or a neighboring) gene or is ... including psoriasis, systemic lupus erythematosus, type I diabetes mellitus and rheumatoid arthritis [11,33-35] Since the CSF1R gene is a target of RUNX1 and has multiple RUNX1 binding sites in several...

Ngày tải lên: 11/08/2014, 10:23

... traditional (classic) CV risk factors (presence/absence) Statistical significance was defined as P ≤ 0.05 Calculations were performed with the statistical package SPSS 15.0 for Windows (SPSS Inc., Chicago, ... Moreover, we assessed whether these two MTHFR gene polymorphisms might be associated with an increased risk of and subclinical atherosclerosis manifested by the presence of endothelial dysfunction ... increased risk of atherosclerosis of patients with RA Conclusions The present study shows for the first time an association of MTHFR A1298C gene polymorphism with the risk of CV events and subclinical...

Ngày tải lên: 12/08/2014, 12:20

... homozygotes would be less susceptible to suppression of IL-17 production by IL-4 compared to I50 homozygotes or heterozygotes We also undertook a pilot cross-sectional study of patients with established ... exclusive) explanation is that in established RA Th17 cells become relatively refractory to IL-4, as we have observed in established CIA [16] To better assess this possibility, it will be necessary ... hours The cells were washed and × 106 cells per sample were used for staining Cells were first blocked with 20 μl of 10% human serum/10% mouse serum in PBS at 4°C for 15 minutes The cells were surface-stained...

Ngày tải lên: 12/08/2014, 15:22

... healthy staff members served as a control group Severity of illness was characterised with the Sequential Organ Failure Assessment (SOFA) scoring system [16] and the Simplified Acute Physiology Score ... pressure (MAP) and central venous pressure (CVP) in mmHg Noradrenaline dosage was measured in µg/minute SOFA, Sequential Organ Failure Assessment score; SAPS, simplified acute physiology score; ... with sepsis, although NOS inhibition ameliorates pathogenic vasodilation and lessens vasopressor requirement, the overall effect is to compromise survival [7] This suggests, in the context of severe...

Ngày tải lên: 13/08/2014, 03:20

... differences in CA3 mRNA expression (Fig 1) as shown by t-test analysis (P > 0.05) An expression pattern was also performed on the skeletal muscle of both Western Yorkshire and Chinese Meishan pigs at ... breeds were analysed MATERIALS AND METHODS 2.1 Analysis of CA3 expression levels Total RNA was extracted using TRIzoL reagent (Invitrogen, Carlsbad, CA, USA) from the longissimus dorsi of Yorkshire ... Studies have shown that the expression of the CA3 gene is strictly tissue-specific and is high in skeletal muscle and much lower in cardiac and smooth muscles [9] The porcine CA3 gene has been assigned...

Ngày tải lên: 14/08/2014, 13:22

... co-repressors such as histone deacetylases (HDACs) and the polycomb repressor, EZH2, which are implicated for cancer progression, are also commonly over-expressed in prostate cancers Interestingly, several ... each respective co-repressor Importantly, we assigned a functional role for the co-repressors in facilitating metastasis Our results showed that ERG, HDACs and EZH2 transcriptionally suppressed ... Huggins and Hodges, 2002) Clinically, most hormone naïve prostate cancers were shown to regress in response to ADT However, recurrence is common with the disease progressing into aggressive, metastatic...

Ngày tải lên: 09/09/2015, 10:14

... of STARS to induce SRF-dependent transcription in NIH 3T3, COS1 and 293T cells However, STARS does not alter the level of expression of MRTFs These observations suggest that STARS stimulates SRF-dependent ... Flightless I NLS Actinremodeling proteins Involved in gel-to-sol transformations; severs and caps polymeric actin filaments; acts in the actin-scavenging system; inhibits actin polymerization Possess ... were shown to serve as a link between STARS and SRF In NIH 3T3 cells cotransfected with expression plasmids encoding MRTFs and STARS, the MRTFs are translocated to the nucleus in the absence of serum...

Ngày tải lên: 07/03/2014, 01:20

... expression of stress-responsive proteins For example, overexpression of Ku-70 and Ku-80 or Ku-70 alone specifically inhibits HSP70 expression [56], whereas overexpression of Ku-80 alone suppresses ... Ku-80 [57] This is the first report to show that Ku-80 expression is suppressed effectively through cell-line transfection In several studies, it has been reported that cells expressing truncated ... the possibility of sequence-independent binding of Ku protein to double-stranded DNA ends The purified proteins were of the same molecular sizes as those shown by silver staining of an SDS/polyacrylamide...

Ngày tải lên: 17/03/2014, 23:20