miniaturized assays of angiogenesis in vitro

... combination of tighter binding of cadmium than zinc in metallothioneins and the preference of zinc in the more flexible b-domain In addition to the already described differences in flexibility of ... blocking of certain nitrosation sites Surprisingly, we did not observe any changes in the TOCSY NMR spectra upon the addition of GSH (data not shown), which is indicative of no specific binding ... cytotoxic and DNA-damaging effects of nitric oxide [29] So, while the binding of NO to GSH in vivo does not obviously prevent NO from interacting with MT2, we have shown that in vitro it suppresses...

Ngày tải lên: 07/03/2014, 15:20

... concentration of [2-14C] creatinine in the apical compartment in the absence of quinidine Creatinine transport was significantly (p < 0.05) inhibited in the presence of quinidine at 30 minutes The ... creatinine in the presence or absence of acyclovir (22 to 89 μM) In contrast, there was a decrease in the concentration of [2-14C] creatinine in the apical compartment in the presence of quinidine ... Creatinine continued to increase following acyclovir discontinuation Creatinine returned to normal within week Elevated urea Mild proteinuria High urea Urinary a1-microglobulin and albumin Creatinine...

Ngày tải lên: 18/06/2014, 16:20

... reaction Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for ... Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a time course of I7L-mediated ... detection of protein In vitro cleavage assay Reactions were performed at the indicated temperature in a final volume of 20 µL containing µL of substrate, 13 µL of enzyme extract, and µL of 20 mM...

Ngày tải lên: 19/06/2014, 08:20

... cortical bone longitudinal modulus of elasticity increases with increase in strain rate by a factor of 1.5 for strain rate of 1500 compared to 1.0 (1/sec), thus explaining in part the greater stiffness ... time in storage], test loading [strain] rate, relative stiffness of the potting-fixtures-testing machine [Kpft] to actual stiffness of the specimen [Ksa], fixture constraints Figure 6of the A) intact ... potting of the ends could be used to test a specimen in all three modes of loading: axial compression, torsion and bending In addition the bending fixtures were capable of applying uniform 4-point...

Ngày tải lên: 20/06/2014, 01:20

... reaction Effect of4 Figure inhibitors on in vitro processing Effect of inhibitors on in vitro processing Various concentrations of protease inhibitors were added to the in vitro processing assay for ... Processing Kinetics of Core Protein Precursors To determine the optimal temperature and kinetics of processing of the core protein precursors in the in vitro cleavage assay, a time course of I7L-mediated ... detection of protein In vitro cleavage assay Reactions were performed at the indicated temperature in a final volume of 20 µL containing µL of substrate, 13 µL of enzyme extract, and µL of 20 mM...

Ngày tải lên: 20/06/2014, 04:20

... ability of various assays in discriminating the subtle difference in the level of effect those nanoparticles could impose to cells Another consideration in assessing the sensitivity of the cell ... Dulbecco's minimum essential medium [DMEM]: Ham's F-12 media containing 10% heat inactivated foetal bovine serum [FBS], mM L-glutamine and 1% penicillin streptomycin [Penstrep] (all purchased from Invitrogen ... Our findings suggest that none of these three assays were suitable for measuring the cytotoxicity of the nanoparticles studied In contrast to Häfeli's findings [10], MTS and Titer-Blue assays...

Ngày tải lên: 20/06/2014, 20:20

... ability of various assays in discriminating the subtle difference in the level of effect those nanoparticles could impose to cells Another consideration in assessing the sensitivity of the cell ... Our findings suggest that none of these three assays were suitable for measuring the cytotoxicity of the nanoparticles studied In contrast to Häfeli’s findings [10], MTS and Titer-Blue assays ... a number of factors including an increase in the coating diameter upon addition of the PEG moiety [33], thus increasing the stability of the nanoparticles and further shielding the cells from...

Ngày tải lên: 21/06/2014, 17:20

... above Figure 2) Here, we have examined the effects of the combined treatment modality of ionizing radiation with the VEGFreceptor tyrosine kinase inhibitor PTK787 in both a spontaneous and a strongly ... results in a supraadditive treatment response Insofar our studies are of high clinical interest since PTK787 exerted a similar treatment response in the allograft and the spontaneously growing tumor ... participated in the in vivo studies MP conceived of the study, participated in its design and coordination and finalized the manuscript All authors read and approved the final manuscript Competing interests...

Ngày tải lên: 09/08/2014, 09:20

... al.: Dense genome-wide linkage analysis of rheumatoid arthritis, including covariates Arthritis Rheum 2004, 50:2757-2765 Horton MA: The αv β3 integrin ‘vitronectin receptor’ Int J Biochem Cell Biol ... because of its high frequency in healthy controls However, it may be another important step on the way to a better understanding of RA etiology and pathomechanisms, in particular the role of angiogenesis ... Haenszel W: Statistical aspects of the analysis of data from retrospective studies of disease J Natl Cancer Inst 1959, 22:719-748 de Vries RR, Huizinga TW, Toes RE: Redefining the HLA and RA association:...

Ngày tải lên: 09/08/2014, 10:21

... fibrosis in skin and reduced inflammation in lungs induced by bleomycin HE stains of mouse skin tissues (Figure 3-1) showed that four-week injections of bleomycin induced significant fibrosis in skin ... reduction of collagen in the lung tissues of bleomycin-induced mice (Figure 4-2) In bleomycin-induced mice, collagen content of lung tissues was 3.6-fold higher than that in salineinjected control ... herein were the very popular bleomycin-induced skin and pulmonary fibrosis in mice Subcutaneous injection and intratracheal instillation of siRNAs were used for tissue-specific treatments of skin...

Ngày tải lên: 12/08/2014, 12:20

... Poly(I:C) induces a dose dependent influx of inflammatory cells into the airways of mice Poly(I:C) induces a dose dependent influx of inflammatory cells into the airways of mice Mice were administered ... of mouse tracheas to poly(I:C) in vitro increases the expression of bradykinin B1 and B2 receptors on the smooth muscle and confers AHR to bradykinin [25] Notably, inhibition of the bradykinin ... acetylcholineinduced AHR following allergen sensitization and challenge [26] In contrast, AHR to histamine following parainfluenza-3 infection in guinea pigs was inhibited by a bradykinin B2 receptor...

Ngày tải lên: 12/08/2014, 14:20

... property of adherence Cells were incubated in 20% Dulbecco's modified Eagles medium (DMEM, Invitrogen UK) + 1% Glutamine + 1% Penicillin/Streptomycin (Invitrogen UK) for hour at 37°C in 5% CO2 ... significant increases in cytokine synthesis in all three types of macrophage In addition, there appears to be a There was considerable variation in the amount by which secretion of an individual cytokine ... Ltd., UK, 0.3% in 100% methanol) for minutes before immersing in Giemsa solution (Raymond Lamb ltd., UK, 1% in pH 6.4 buffer) for a further 20 minutes Cytospins were then rinsed in pH 6.4 buffer,...

Ngày tải lên: 12/08/2014, 14:20

... determined in the current study, in the joint space With respect to the role of Il-1 signalling in OA, in the one human clinical study using recombinant IL-1ra as a treatment for OA, a single injection ... IL-1ra: interleukin-1 receptor antagonist; IL-1β: interleukin-1 beta; IL-4: interleukin-4; IL-6: interleukin-6; IL-10: interleukin-10; IL-13: interleukin-13; MMP: matrix metalloproteinases; NO: ... in vitro, suggesting that, if any, in vivo effects of the TNFα in the injected ACS would have been indirect It is not clear to what extent the increased levels of IL-6, OSM and lower levels of...

Ngày tải lên: 12/08/2014, 14:22

... WJA, Creemers LB: Cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection Arthritis ... ACL surgery-preliminary report Int Orthop 2009, 33:413-418 Yang KG, Saris DB, Verbout AJ, Creemers LB, Dhert WJ: The effect of synovial fluid from injured knee joints on in vitro chondrogenesis ... diffrences from asymptomatic subjects in systemic markers of inflammation suggesting differences in innate immunity Paper presented at: Osteoarthritis Research Society International 2010 World Congress...

Ngày tải lên: 12/08/2014, 15:22

... and participated in the clinical characterization of patients as well as in obtaining informed consent HM provided the funding of the study and participated in the preparation of the manuscript ... replicative ageing of human endothelial cells J Cell Sci 2000, 113(Pt 20):3613-3622 Severino J, Allen RG, Balin S, Balin A, Cristofalo VJ: Is beta-galactosidase staining a marker of senescence in vitro ... covered a number of genes of diverse pathways that could be altered in emphysema or cellular senescence LOXL2 seemed of interest as involved in cross-linking collagens and elastin [24]; it has...

Ngày tải lên: 12/08/2014, 16:20

... molecular transport of each isoform in the interstitial space, binding of VEGF 164 to HSPG in the ECM, VEGF164/120 binding to VEGFR2 at the endothelial cell surface and internalization of these ligand-receptor ... molecular factor involved in angiogenesis, including exercise-induced angiogenesis Among several splice isoforms in the VEGF family, VEGF120 and VEGF164 (in rodents; human isoforms are VEGF121 ... formation of a first stalk cell segment ii) Following invasion of new sprouts into the tissue by extending the leading tip and stalk cell segments, the tip cell continues to migrate in the interstitial...

Ngày tải lên: 13/08/2014, 16:20

... conditions Effects of coconut water and sucrose on flowering induction Sporad analysis of pollinia Germination of pollen grains in vitro In vitro pollination and seed production in culture Results ... juvenile phase in orchids, and to induce flowering in vitro in order to achieve flowering in a shorter period of time To date, flowering in vitro has been successfully induced in Cymbidium (Kostenyuk ... importance of cytokinins in flowering was mainly observed in field experiments involving foliar spray or injection of cytokinins (Sakai et al., 2000; Blanchard and Runkle, 2008) The objective of this...

Ngày tải lên: 11/09/2015, 10:06

... for the development of in vitro models.  19 1.4 Role of inflammation in drug-induced nephrotoxicity in humans In the development of in vitro models for the prediction of drug-induced nephrotoxicity, ... HPTC-based in vitro model for nephrotoxicology developed here employed drug-induced increases in mRNA expression levels of the pro-inflammatory interleukins (IL)-6 and IL-8 as endpoint Pro-inflammatory ... validation of endpoints suitable for in vitro prediction of drug-induced nephrotoxicity in humans 57 4.3.1 Model design and identification of suitable endpoints for in vitro nephrotoxicology...

Ngày tải lên: 30/09/2015, 06:22

... taining After 48 h, germination was scored based on the percent of grains in each of categories: Class 1, pollen grains elongated greater than twice the original hydrated diameter of the grain; ... the electrode was inserted making certain all air bubbles were excluded, and stirring resumed Oxygen uptake was recorded for a minimum of using a chart speed of cm/min The rate of oxygen consumption ... value of 0.0083 For comparing the effect of hydration on assay response, a paired t-test was used = For in vitro assays, the experimental unit was as a family of pollen grains arising from a single...

Ngày tải lên: 08/08/2014, 23:22

... [16-18] In this context, IL-8 has been involved in the expression of VEGF in endothelial cells [19], induction of angiogenesis [20], tumor angiogenesis in gastric cancer [13], and acquisition of chemotherapeutic ... Mondalek et al., Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP Journal of Nanobiotechnology ... against human melanoma cells in vitro and in vivo [30] In this context, we investigated the ability of DCPIP and nanoparticle-encapsulated DCPIP to affect the expression of factors/cytokines inducing...

Ngày tải lên: 11/08/2014, 00:22