mimetic peptide surfaces and scaffolds to modulate cell behavior

... is found on cell surfaces of many types of cultured cells [19–21] Indeed, flow cytometric analysis revealed the existence of annexin II on cell surfaces of WiDr cells (data not shown) To examine ... annexin II with the cell surface receptors Binding of tPA to surfaces of matrilysin-treated cells It has been shown that tPA binds to annexin II on the surfaces of human endothelial cells [18,25] In ... (MAT) on 96-well plates for h, and incubated with tPA and TAPI-1 as above To quantify tPA bound to the cell surface, the cultures were subjected to cell ELISA according to the method described in...

Ngày tải lên: 17/03/2014, 17:20

... effort to circumvent these problems by designing strategies to deliver full-length proteins into a large number of cells Morris Group [7] designed and synthesized a new type of cell penetrating peptide ... supernatants and cells were collected respectively after treatment Annexin V and propidium iodide (PI) binding assay To measure H9c2 cell apoptosis after hypoxia-reoxygenation treatment, we labeled the cells ... Medicine and housed at an appropriate temperature (25°C) and relative humidity (55%) with a fixed 12 h light/dark cycle and free access to food and water The animals were randomly divided into five...

Ngày tải lên: 18/06/2014, 19:20

... binding and presentation to CD8 + cytolytic T cells In HFE, the proximity of the two a helices and the presence of amino acid side chains that project into the groove appear to prevent peptide ... Dublin, Ireland) and phosphatase inhibitors (1 mm sodium vanadate and sodium fluoride) Cleared cell lysates were fractionated on 11% SDS/polyacrylamide gels and transferred to nitrocellulose membranes ... established that in certain cell types HFE acts to regulate intracellular iron levels by decreasing the binding of transferrin to transferrin receptors and reducing cellular iron uptake as a consequence...

Ngày tải lên: 06/03/2014, 22:21

... Biotech Monitoring T cell division and early expansion using CFSE labeled cells To monitor cells division and expansion during the early days after stimulation, cells were CFSE-labeled and monitored ... extensive anti-CD3-T cell receptor crosslinking on a surface, and the costimulation provided by cell- cell interaction between T cells and Fc receptor positive accessory cells such as monocytes ... to maintain cell numbers between 0.75 and × 10 /ml Because of the presence of irradiated autologous feeder cells in REP treated cells, viable cell counts were not used to monitor cell growth...

Ngày tải lên: 18/06/2014, 16:20

... possible to use clinical-grade lentiviral vectors in order to redirect polyclonal Treg cells to the specific targets, as well as to prevent Treg cell conversion to the Teff cells [94] Thus, Treg cells ... to be examined Page of 12 In humans, exposing CD4+ T cells to both IL-2 and rapamycin in vitro leads to an increase in the cellular frequency of FOXP3+ T cells, originating from nTreg cells and ... cytokine receptors, expressed by many cell types and tissue, whereas the IL-2Ra expression is mostly restricted to activated T cells, and Treg cells [14] In recent years, strong genetic and molecular...

Ngày tải lên: 18/06/2014, 16:20

... inflammatory responses are often a two-edged sword Fc receptor binding is known to enhance the activation and pro-inflammatory secretory responses of scavenger cells that bear Fc receptors, and microglia ... distances more proximal to Aβ aggregates In addition, microglia are now well established to express receptors that can mediate chemotactic behaviors and that appear to have Aβ as a ligand These include ... [12] and those of others [25-27] with TNF-α, IL-6, and a broad range of chemokines, cytokines, and inflammatory toxins such as reactive oxygen/nitrogen species Pathways for enhancing TNF-α and...

Ngày tải lên: 19/06/2014, 22:20

... contributes to ovarian tumor cell survival Methods Cell Lines and cell culture Human OvCa cell line, OVCAR-3, was obtained from the ATCC The cells were cultured in RPMI 1640 (Mediatech, Inc.) at 37°C and ... antibodies for 24 hours The cells were harvested and washed in cold PBS and the cell density was adjusted to 106 cells/ml Subsequently, cells were stained with Annexin V and Propidium Iodide (PI) ... SKOV-3 cells were switched to RPMI-1640 with 10% FBS Prior to each experiment, cells were cultured for 24 hours in RPMI 1640 and 2% charcoal-striped FBS Cell Proliferation Assay OvCa cells (105)...

Ngày tải lên: 20/06/2014, 07:20

... [8,9] Apoptosis is a physiological process and is a highly selective way to eliminate aged and injured cells In addition to internal pathways that trigger apoptosis mainly in response to cytotoxic ... Takeno M, Saito N, Yokoe T, Kaneko A, Asai T, Sakane T: Modulation by proinflammatory cytokines of Fas/Fas ligand-mediated apoptotic cell death of synovial cells in patients with rheumatoid arthritis ... fibroblast-like cells, and alterations in the susceptibility of such cells to Fas-induced cell death have been strongly implicated in the pathogenesis of inflammatory joint diseases such as rheumatoid arthritis...

Ngày tải lên: 09/08/2014, 01:23

... Sensitivity and specificity as diagnostic indicators for rheumatoid arthritis for antibodies against cyclic citrullinated peptide (anti-CCP Ab) and for rheumatoid factor (RF) of IgA, IgM, and IgG ... haplotypes leads to the loss of self-tolerance followed by polyclonal activation of T and B cells and the subsequent production of autoantibodies [9] This mechanism could be applicable to autoantibodies ... SE- and IgA-RF+ 11 12.3 3.6–41.7 SE+ and IgA-RF+ 14 39.2 7.9–193.9 SE- and IgM-RF- 18 71 1.0 SE+ and IgM-RF- 26 38 2.6 1.2–5.2 SE- and IgM-RF+ 5 3.7 0.99–14.3 SE+ and IgM-RF+ 14.9 2.9–76.3 SE- and...

Ngày tải lên: 09/08/2014, 01:23

... nitrogen storage and transport and here contributed to cell π, especially in the mature zone of the tap root Inorganic ions, soluble sugars and glutamine accounted for about two third of cell π ... to those in our previous work [23] and concentrations of inorganic ions, soluble sugars and amino acids were determined on a water basis in different parts of the root system and related to cell ... were pooled together In total, 56, 58 and 42 seedlings were used for the control, KD and MD treatments, respec- injector (Gilson 222 XL, Villiers le Bel, France) Guard column AG12A and column...

Ngày tải lên: 09/08/2014, 04:20

... FcγR-mediated phagocytosis (FcγRIIa-131R and FcγRIIIa-158F) and impaired antigen-dependent cellular cytotoxicity of IgG-opsonised cells (FcγRIIIa-158F) Alternatively, FcγR polymorphisms may modulate endothelial ... to play important roles in the initiation and regulation of many immunological and inflammatory processes and to amplify and refine the immune response to an infection Activating FcγRs (FcγRI, ... additionally stimulates antigen-dependent cellular cytotoxicity by natural killer cells Page of (page number not for citation purposes) Giant cell arteritis patients and controls This was an allelic association...

Ngày tải lên: 09/08/2014, 08:22

... leading to cytokine secretion and cytotoxic activity After the expansion ex vivo of NK cells in culture, it is crucial to determine whether NK cells continue to kill target cells in response to stimuli ... Thus, NK cells were tested with respect to their cytotoxicity to K562 target cells Seven days of expansion with the different cytokine regimens did not reduce the cytotoxicity to K562 target cells ... transduction pathway modulate the expression of the different receptors on NK cells to preserve an equilibrium, maintaining self-tolerance and the capacity to produce cytokines or to be cytotoxic By placing...

Ngày tải lên: 09/08/2014, 10:22

... Clinical Guide to Stem Cell and Bone Marrow Transplantation Page Introduction to Stem Cell and Bone Marrow Transplantation The pluripotent stem cells from which all committed blood cells arise (Figure ... enough—our husbands Daniel, Dan, and Keith, and our children Alexandra, Derek, and Leah, who lent support and sanity in our times of need we love you! Page vi Preface As the science and technology ... small cleaved cell Follicular mixed small cleaved and large cell Intermediate grade Follicular predominately large cell Diffuse small cleaved cell Diffuse mixed small cleaved and large cell High...

Ngày tải lên: 10/08/2014, 08:20

... fractionated TBI, 60 mg/kg of etoposide was found to be the maximum tolerated dose; stomatitis and hepatic tolerance were the dose-limiting toxicities.3 It was also shown that 110 to 180 mg/m2 of melphalan ... the sequencing of cytotoxic drugs in relation to TBI.3, The changes were made to minimize some of the toxicity-related symptoms that patients experienced C Dose escalation and TBI Dose escalation ... GVHD risk factors) C Medical evaluation Complete medical history Attention to chronic medical problems Medications Pregnancy history (female) Anesthesia history Transfusion history History of blood...

Ngày tải lên: 10/08/2014, 08:20

... started around day +7 to +8 posttransplant c) Interleukin-3 (IL-3) is used to stimulate the earliest progenitor cells of all cell lineages (red cell, platelets, and white cells) May decrease the ... processed to remove red blood cells (in the case of ABO incompatibility) or purged to remove either T cells or malignant cells D T -cell depletion involves removal of donor T cells prior to an infusion ... factor support to minimize fever and potential for infection as well as to enhance the mobilization effect Generally, stem cell apheresis is scheduled to begin as the peripheral white blood cell...

Ngày tải lên: 10/08/2014, 08:20

... Mycoplasma, and Legionella e) Gastrointestinal: stool for CMV antigen and culture Stool should always be sent for ova and parasites and C difficile toxin with any diarrheal Page 171 illness Stool for ... viral illness and conditioning-related toxicity, the stool tends to have decreased [Na] and increased stool osmotic gap (> 100 mOsm/L) f) Gastrointestinal endoscopy with culture and biopsy (rule ... Pyrazinamide Tablet: 500 mg 15–30 2g mg/kg/d PO Hepatotoxicity, hyperuricemia Streptomycin Vial: g, g IM 20–40 mg/kg/d 1g Ototoxicity, nephrotoxicity Ethambutol Tablet: 100, 400 mg PO 15–25 mg.kg/d 2.5...

Ngày tải lên: 10/08/2014, 08:20

... syndrome) and bladder involvement (severe cystitis) r) Hematopoietic system: Eosinophilia and thrombocytopenia, platelet-bound autoantibodies, autoimmune hemolytic anemia, and reduced hematopoietic ... necrosis factor and appears to reduce the number of transplantrelated complications, including acute GHVD c) Cytokine antagonists: Cloned soluble receptors for a number of interleukins and cytokines ... decreased sodium and increased stool osmotic gap (> 100 mOsm/L) d) Stool for fecal leukocytes: Leukocytes are indicative of bacterial infections e) Stool for ova and parasites and stool culture for...

Ngày tải lên: 10/08/2014, 08:20

... malabsorption d) Stool electrolytes: Secretory diarrhea tends to have an elevated stool sodium and a lower osmotic gap (< 100 mOsm/L) In malabsorption or viral illness, the stool tends to have a decreased ... amount of fecal material to a drop of saline mix, remove feces with an applicator stick, and cover Examine the specimen under low power to locate the parasites and high power to identify the parasites ... required to support the patient (e.g., ventilator), it may be difficult to perform the scan D Bone scan Method: An injection of an isotope, usually technetium 99m, to image the bony skeleton The...

Ngày tải lên: 10/08/2014, 08:20

... administration and and h after each dose (refer to individual protocol) Class uroprotectant Dose and route Special considerations Cyclophosphamide: 20% to 100% w/w of dose prior to administration and 3, ... asthenia (Paraplatin) BMT dose; 1500–2000 Dose-limiting toxicity: peripheral neuropathy, mg/m2 (consult protocol) hepatotoxicity, nephrotoxicity,ototoxicity Class: alkylating agent Carmustine (BCNU) ... BMT dose: 150–240 Dose-limiting toxicity: nephrotoxicity, ototoxic mg/m2 (consult protocol) Cyclophosphamide Tab 25, 50 mg Conventional dose: 500–1500 mg/m (Cytoxan) Injection: 500 mg BMT dose:...

Ngày tải lên: 10/08/2014, 08:20