migration storage and recall of information in entire plants

... enabling these seedlings to recall stored information of hypocotyl-elongation inhibition 5.2.2 Breaking the Symmetry of Bud Growth in Bidens Seedlings Asymmetry-Index, g, of a Set of Bidens Seedlings ... degradation and that lithium inhibits the peptidic biosynthesis that normally compensates for this degradation 5.2 5.2.1 Migration, Storage and Recall of Information in Entire Plants Inhibition of Hypocotyl ... junctions of most cells and to covalent bonding in the internal part of the wall of the epidermal cells Both types of bond were coexisting in the intercellular junctions of the cortical parenchyma In...

Ngày tải lên: 29/05/2014, 23:58

... yeast strain, which is deficient in Ras signaling and regains it with the presence of interacting protein pairs The machinery of intrinsic cell survival and proliferation of Ras signaling is utilized ... and the obtaining of conformational information [26–28] Thus, yeast is definitely an attractive organism for analyzing the interactions of eukaryotic proteins Guanine nucleotide-binding proteins ... C-terminus of cytoplasmic protein X, and the protein X–Gc fusion protein and integral membrane protein Y in its native form were coexpressed in a ste18D strain The interaction between protein X–Gc...

Ngày tải lên: 06/03/2014, 11:20

... the design of and screening for small binding proteins The preparation of the small binding proteins with binding function further allows us to increase the binding strength by multibinding approaches, ... Replacement of the candidate location in NCS for grafting with the CDR loop of VHH (B) Insertion of the CDR 1-containing loop of the heavy chain in Fv into the candidate location in TOP 2008 ... A-domains consisting of  35 amino acids occur in strings of multiple domains in several cell surface receptors, and are connected via several amino acid linkers Each A A-domain in the multimer binds...

Ngày tải lên: 06/03/2014, 11:20

... (CDS) within 70 bp of an exon-intron junction Genes were grouped into bins of nine equal intervals according to the proportion of sequence within 70 bp of an exon-intron junction, and the SNP ... artifacts of the data sets [63] We obtained human protein-protein interaction data from the IntAct database [64] and examined how SNP A/S ratios are correlated with the connectivity of proteins in the ... variance in the CGC set resulting from a smaller number of genes Selective constraints and protein-protein interaction It is still debatable whether there is any correlation between protein-protein interaction...

Ngày tải lên: 14/08/2014, 08:21

... identification of binding partners for a known protein by expressing the protein in fusion with BD domain and then screening proteins in fusion with AD It also allows the identification of specific binding ... domain-containing adaptors and TLR signaling MyD88 is an essential TIR domain-containing adaptor for the induction of inflammatory cytokines via all the TLRs TIRAP/Mal is a second TIR domain-containing ... domain in its C-terminal portion and a death domain (DD) in its Nterminal portion Activation of TLR or IL-1R recruits MyD88 to the receptor The TIR domain of MyD88 binds to the TIR domains of...

Ngày tải lên: 12/09/2015, 08:20

... TAO introduces striking conformational rearrangements in the exit tunnel [60] by flipping the tip of the b-hairpin of protein L22 (Fig 6) Protein L22 consists of a single globular domain and a ... with guanine in position 2058 are somewhat less selective for the binding of 15- or 16-ring macrolides It was found that the binding mode of 15-ring macrolides to ribosomes with guanine in position ... Erythromycin and TAO (F) Side view of the upper region of the tunnel, showing TAO binding site and the native and the swung conformations of L22 b-hairpin tip A modeled poly(Ala) nascent chain is...

Ngày tải lên: 23/03/2014, 17:22

... of MS During primary turnover, the homocysteine-binding domain (dotted barrel) and the CH3-H4-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark ... (light grey) and the C-terminal activation of hMS (grid-barrel) interact with the cobalamin-binding domain For more information on hMS conformational substates, see [5] and [33] cob(II)alamin], an ... structure of B12-binding domains of methionine synthase Science 266, 1669–1674 Dixon MM, Huang S, Matthews RG & Ludwig M (1996) The structure of the C-terminal domain of methionine synthase: presenting...

Ngày tải lên: 18/02/2014, 08:20

... corresponding to the PIN domain, was removed from the entire coding sequence of the MCPIP gene The coding sequence lacking the PIN domain was obtained in two steps First, the fragment of 423 bp ... proteins interacting with MCPIP will clarify the dynamics and features of this protein Role of MCPIP in regulation of the endogenous IL-1b transcript level After identification of a PIN domain in the ... amino acids located within the PIN domain (D141A and D226A) Transcription was inhibited by addition of actinomycin D, and RNA was collected after 3, and h The mRNA level for IL-1b (top panel) and...

Ngày tải lên: 18/02/2014, 14:20

... (enzyme I of the PTS), the rst protein in the cascade of proteins forming the PTS, to HPr, the histidine-phosphocarrier protein HPr is the smallest protein in the protein cascade of the PTS and it ... HPr of S coelicolor, scHPr, contains 93 amino acid residues; it lacks cysteine and tyrosine residues, and it only contains one tryptophan and one phenylalanine residue Assignment and preliminary ... the propagation of tting errors DCp was obtained from tting of the thermal- and urea-denaturation data (using the approach of Pace and Laurents [39]) Indicated errors are tting errors to Eqn...

Ngày tải lên: 19/02/2014, 12:20

... properties In this work we took advantage of the specic labeling with PLP of Lys288 in E coli PEP carboxykinase to gain insight into conformational alterations of the protein upon ligand binding in solution ... free OAA binds in the second coordination sphere of Mn2+ [3] Binding of CO2 is not affected by the presence of cations, indicating that the interactions of Mn2+ and CO2 are independent of each ... acquisition of detailed and reliable information on the binding of several ligands and on the role of Mn2+ and Mg2+ on their binding The comparison between acrylamide quenching studies and modeled...

Ngày tải lên: 21/02/2014, 01:21

... protein, may be useful in testing our theoretical conclusions The integration of experimental information with modeling approaches will certainly provide new insights into the intramolecular and intermolecular ... between kinases and their targets In place of enzyme kinetics, the mathematical description of elementary reaction and binding steps is feasible but introduces a large number of variables and parameters, ... by a single kinase We conclude that knowledge concerning phosphorylation order and the properties of protein–protein interactions is crucial for the elucidation of the steadystate and kinetic...

Ngày tải lên: 07/03/2014, 10:20

... changes in the labeling intensity of the apparent 94 and 560 kDa bands after days of electro-stimulation, and a drastic decrease in the decoration of the 63, 94 and 560 kDa bands after 78 days of ... a tendency of reduced linkage of CSQ to TRI, JUN and the RyR1 in senescent fibers (Fig 5B) Immunoblotting of TRI in both ageing and developing microsomes showed weak and broad labeling patterns ... TRI and the CSQ-binding element JUN, show decreased protein–protein interactions during fiber type shifting and the ageing process Reduced protein coupling between the major elements regulating...

Ngày tải lên: 08/03/2014, 10:20

... of a tetramer after binding of GppNHp, and binding of GTPcS again shows an intermediate behavior (Fig 5B) The elution profiles of both hGBP5ta and hGBP5a ⁄ b in the presence of various nucleotides ... features of different members of the human guanylate-binding protein family J Interferon Cytokine Res 27, 44–52 Rupper AC & Cardelli JA (2008) Induction of guanylate binding protein by gamma interferon ... (2006) In silico genomic analysis of the human and murine guanylatebinding protein (GBP) gene clusters J Interferon Cytokine Res 26, 328–352 Cheng YS, Colonno RJ & Yin FH (1983) Interferon induction...

Ngày tải lên: 15/03/2014, 10:20

... C-terminal of the DNA-binding domain (amino acids 98–240) Lin et al [19] mapped the minimal C-terminal domain to amino acids 380–427, and our results suggest that maximal interaction involves amino ...  24 and 36% of input for IRF-3E5 and E7, respectively Binding to the corresponding regions of p300 was approximately 2–3 times weaker When binding of IRF-3 to smaller domains of the N- and C-terminal ... competing for the intra- or intermolecular interaction involving phospho-Ser385/386 and another domain of IRF-3, preventing dimerization and hence interaction with p300/CBP CONCLUSIONS In summary,...

Ngày tải lên: 17/03/2014, 10:20

... switch serines 73 and 138 and threonine 153 of the HIP/PAP protein for alanines Oligonucleotides cassettes containing the desired mutations were inserted into pcDNA3-HIP/PAPmyc-His as indicated ... serine and serine 12 in vivo and by casein kinase I J Biol Chem 268, 26712–26718 36 Cowles, E.A., Agrwal, N., Anderson, R.L & Wang, J.L (1990) Carbohydrate-binding protein 35 Isoelectric points ... consequences of HIP/PAP expression in hepatocellular carcinoma, we looked for proteins capable of interacting with this protein by screening a human HCC cDNA expression library in kgt11 using [32P]chimeric...

Ngày tải lên: 23/03/2014, 13:20

... R.S (1997) Mapping of a second actin-tropomyosin and a second troponin C binding site within the C terminus of troponin I, and their importance in the Ca2+-dependent regulation of muscle contraction ... residue of the troponin I inhibitory sequence 104–115 in the interaction with troponin C and tropomyosinactin J Biol Chem 263, 1726–1732 19 Wilkinson, J.M & Grand, R.J.A (1978) Comparison of amino ... the Ca2+-induced proximity changes of Cys48 and Cys64 in TnI during Ca2+ binding to the ternary complex of TnI–TnC–TnT In addition, the amino acid sequences of skeletal and cardiac TnC and TnI...

Ngày tải lên: 23/03/2014, 21:20

... preceding the a-helix and the C-terminal b-strand of RBP and the hyper-variable regions of Fab: loops 53–56 and 100–103 and the short helix 28–32 of chain H and loops 31–36 and 53–56 of chain L ... transthyretin J Mol Biol 366, 711–719 Transthyretin–retinol-binding protein interactions 40 Kopelman M, Cogan U, Mokady S & Shinitzky M (1976) The interaction between retinol-binding proteins and prealbumins ... Transthyretin–retinol-binding protein interactions G Zanotti et al Transthyretin (TTR), a homotetramer of approximately 55 kDa, is a thyroid hormone-binding protein present in the extracellular fluids of...

Ngày tải lên: 30/03/2014, 02:20

... (1994) Binding of a member of the NF1 family of transcription factors to two distinct cisacting elements in the promoter and 5'-flanking region of the human cellular retinol binding protein gene ... component of the DNAprotein complex, whereas the protein binding surface can be probed by amino acid side chain modification, DNAprotein crosslinking, and of course by the production of protein mutants ... Assays for the Analysis of DNAProtein Interactions Marc-Andrộ Laniel, Alain Bộliveau, and Sylvain L Guộrin 13 DNase I Footprinting Benoợt Leblanc and Tom Moss 31 Footprinting with Exonuclease...

Ngày tải lên: 08/04/2014, 12:51

... intracellular and intercellular trafficking in plants Plant Cell 1999, 11:535-548 Gafni Y, Epel BL: The role of host and viral proteins in intraand inter-cellular trafficking of geminiviruses Phys ... compromised in both respects These observations provide strong evidence in support of the importance of specific mp-cp interactions in natural infections of maize Specific binding of MP and CP has ... http://www.virologyj.com/content/5/1/61 In the small and informationally compact MSV genome, deletion or inactivation of any genes results in asymptomatic infections or loss of infectivity [13,14] and in some cases even...

Ngày tải lên: 20/06/2014, 01:20