microrna expression in disease

Ngày tải lên: 18/06/2014, 19:20

Ngày tải lên: 20/06/2014, 03:20

Ngày tải lên: 19/06/2014, 22:20

Ngày tải lên: 20/06/2014, 07:20

... IL5 – the interleukin 5 gene, λ Ig – the λ -chain of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin 4 receptor ... effects of calpains in cancer cells [27-29] suggest that calpain participates in cell malignization by digesting the p53 tumor suppressor. Besides, calpains participate in induction of HIV ... Hodgkin’s disease; capn4: the calpain subunit 4 gene; ND4: the NADH dehydrogenase subunit 4 gene; IL5: the interleukin 5 gene; λ Ig: the λ -chain of immunoglobulin gene; γIg: the γ-chain of...

Ngày tải lên: 02/11/2012, 11:08

... tissue staining intensities influ- enced the measured values. Average value density measurements from individual Vgf immunoreactive dorsal spinal cord neurons, reflecting immunostaining intensity, ... in the serum, in the CSF, and in spinal cord motorneurons may be a biological diagnostic index for ALS. It remains to be directly tested whether restoring Vgf expression in spinal cord motor ... sequencing, and the recombinant virus was packaged by infecting the PacI linearized recombinant viral DNA into human embryonic kidney (HEK)-293 cells (Clontech, CA). The resulting recom- binant...

Ngày tải lên: 03/11/2012, 10:52

... cannot determine the global players involved in such complex phenotypes. Integration of the gene expres- sion profiling with specific modulation of gene ex- pression in relevant signalling pathway ... according the defini- tions of the Illumina’s (ILL) data as well as of the Il- lumina’s annotations (table 2). Int. J. Med. Sci. 2009, 6 http://www.medsci.org 27 weight cargo upon binding ... Genes detected and in following investigated for valida- tion A) an induced expression of the PKN1 gene B) a repressed expression of the ZCD1 gene C) a repressed expression of BSG gene...

Ngày tải lên: 03/11/2012, 11:44

Ngày tải lên: 29/09/2013, 17:10

... protein was released from the chitin column when the chitin-bound intein tag undergoes self-cleavage in the presence of DTT. Fig 12 showed chitin gel before (N1) and after (N2) releasing MCoTI-II. ... Aprotinin 4. Re MCoTI-II Fig 14. Checking the inhibiting activity of ReMCoTI-II by using diffusion method 1. PA from S. litula 2. PA of S. litula + ReMoTI-II 3. Trypsin 4. Trypsin + ReMCoTI-II ... expression: use LB medium containing 100µg/ml ampicillin, incubated in shakers at 37 o C until the OD 600 reached 0.6. Induction by IPTG. Expression and purification of recombinant MCoTI-II were followed...

Ngày tải lên: 12/02/2014, 10:20

... 2009) doi:10.1111/j.1742-4658.2009.06973.x Activating enhancer-binding protein (AP)-2e was previously described as a new regulator of integrin a 10 expression in cartilage. In this study, we ana- lyzed the expression of AP-2e in differentiated ... the Sox9-binding sites of the AP-2e promoter (Sox9_1 and Sox9_2). Incubation of in vitro-synthesized Sox9 with the labeled oligonucleo- tides containing the Sox9-binding sites resulted in a strong ... Max-Planck-Institute of Biochemistry, Martinsried, Germany The family of activating enhancer-binding protein (AP)-2 transcription factors regulate their target genes through binding to the palindromic...

Ngày tải lên: 18/02/2014, 08:20

... of cytokine induced expression of intercellular adhesion molecule 1 (ICAM-1) in an intestinal epithelial cell line, a process blocked by proteasome inhibitors. Gut 42, 779–787. 23 Lakshminarayanan ... to an increase in ICAM-1 expression in Schwann cells of sciatic nerves Aiguo Shen 1, *, Junling Yang 2, *, Yangyang Gu 3 , Dan Zhou 4 , Linlin Sun 2 , Yongwei Qin 2 , Jianping Chen 2 , Ping Wang 2 , ... LPS induces ICAM-1 expression in SCs, suggest that ICAM-1 may play a role in the focal accumulation and antigen-induced activation of T cells in inflammatory demyelinating diseases of the PNS. As...

Ngày tải lên: 18/02/2014, 18:20

... for silver staining, hot Coomassie s taining, ÔStains-AllÕ labelling or immunoblotting. T he following running conditions were used: 60 m in at 1 00 V , 6 0 m in at 500 V, 60 min a t 1000 V, and ... the deficiency in dystrophin o n the expression of Ca 2+ -binding proteins. The relatively unique combination o f the pI value and molecular mass of individual 2D spots can be useful in the initial ... carbocyanine dye ÔStains-AllÕ was u sed to determine potential changes in the expression of major Ca 2+ -binding proteins in dystrophic fibres. A comparison between the selective d ye labelling of...

Ngày tải lên: 19/02/2014, 16:20

... caveolin proteins [22]. Several studies have shown that caveolin, a 21–24 kDa integral membrane protein, plays a critical r ole in the regulation of cell proliferation, including SMC, by inhibiting ... by inhibiting signalling mol- ecules [ 45–47]. Caveolin-3 is the main isoform in muscle cells, including SMC [30]. Caveolin-1 and caveolin-3 seem to act as scaffolding proteins that negatively ... nine passages a fter primary culture. Confluent cells were maintained in quiescent mode by incubation for 24 h in serum-free m edium containing 0.2% (w/v) fatty acid-free BSA. Then, SMC were incubated or...

Ngày tải lên: 19/02/2014, 16:20

... induction of inducible nitric oxide synthase (iNOS), cyclo-oxygenase-2 (COX-2) and MMP-9. In this study, we investigated whether LXR activation inhibits inducible TF expression and activity in ... finally degenerating into a lipid core. Plaque rupture exposes active TF in the lipid core to circulating blood, trigger- ing thrombosis. As the initiator of coagulation, TF is a potential target for inhibiting ... the enhanced expression of in ammatory genes including TF during the development of atherosclerosis induced by the acti- vation of NFjB. The current data suggest that LXRs allow binding of NFjB...

Ngày tải lên: 19/02/2014, 17:20

... from both brain and intestine in L. maderae [14,15]. The finding that N-terminally extended TKRPs are predominantly expressed only in the gut and not in the brain are in line with findings of enrichment ... tachykinins, the hemokinins, were identified on a third precursor encoding gene, preprotachykinin C, expressed in hematopoietic cells of mouse, rat and humans [6,7]. In invertebrates the tachykinins ... the insectatachy kinin ⁄ tachykinin and insect kinin ⁄ Tyr-W-MIF neuropeptide family pairs. Ann NY Acad Sci 897, 388–400. 2Na ¨ ssel DR (1999) Tachykinin-related peptides in inver- tebrates: a...

Ngày tải lên: 20/02/2014, 01:20

... could also remain trapped and inhibit the intrinsic uncoupling activity of hUCP3, the digitonin treated cells were extensively incubated with the resin Dowex-K21. This extracting procedure was ... times in NaCl/P i ,thenfixed in 4% (w/v) paraformaldehyde/NaCl/P i for 60 min. The cells were w ashed four times in NaCl/P i and then i ncubated for 60 min in NaCl/P i containing 0.1 M glycine, ... permeabi- lized using 0.1% ( v/v) Triton X-100 in NaCl/P i for 3 min, followed by four NaCl/P i washes. The slides were then incubated in blocking buffer containing 5% (v/v) fetal bovine serum, 0.1%...

Ngày tải lên: 21/02/2014, 15:20

... TICK FRDM SKIN; APPLICA- TIONS TO SKIN OF TURPENTINE, IODINE, R~'TONE; REMOVAL OF TICK BY INSERTION OF NEEDLE BETWEEN MOUTH PARTS; ASPIRIN FOR PAIN; ANTIBIOTIC TREATMENT IN- ~-~CTIVE. ... in CMIT, in order of increasing 'enT.ropy' The enl=ropy is given in P.he first column; ,':he ent.ries in ~h~ nezt ii columns are ~he percent of occurrences in the 11 disease ... correspond to the disease categories represented in CMIT. This is done by assign- ing terms which occur predominantly in one category to a single vocabulary and then sorting by entropy. Tables...

Ngày tải lên: 21/02/2014, 20:20

... believes in measuring quantitatively something of such an intrinsically subjective nature as self- expression in a discipline so detached from numbers and the right-wrong answer distinction ... teacher started assessing them according to the established marking criteria. Data analysis: Insights into students’ self -expression during the learning process Self -expression problems One ... very active in class discussions: expressing their wonder at the details of the text or disagreement with others’ ideas, defending their opinions convincingly, posing very interesting questions...

Ngày tải lên: 05/03/2014, 12:20

... the present study indicate that adipophilin protein expression in muscle is involved in maintaining insulin sensitivity. Abbreviations Adfp, adipophilin; CLB, classical lysis buffer; FA, fatty ... is implicated in insulin resistance. As shown in the present study, palmitic acid indeed impaired insulin signaling in C2C12 cells, which is in line with numerous studies addressing the effect ... protein expression in muscle is increased, accompanied by indications for better insulin sensitiv- ity. Taken together, the results obtained in the present study indicate that Adfp expression in...

Ngày tải lên: 06/03/2014, 09:22

Ngày tải lên: 06/03/2014, 19:20