... IL5 – the interleukin 5 gene,
λ
Ig – the
λ
-chain of immunoglobulin gene,
γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible
gene 6-16, IL4R – the interleukin 4 receptor ... effects of calpains in cancer cells [27-29] suggest
that calpain participates in cell malignization by digesting
the p53 tumor suppressor. Besides, calpains participate in
induction of HIV ... Hodgkin’s disease; capn4: the calpain
subunit 4 gene; ND4: the NADH dehydrogenase subunit 4
gene; IL5: the interleukin 5 gene;
λ
Ig: the
λ
-chain of
immunoglobulin gene; γIg: the γ-chain of...
... tissue staining intensities
influ-
enced the measured values. Average value density
measurements from individual Vgf immunoreactive
dorsal spinal cord neurons,
reflecting immunostaining
intensity, ... in the serum, in the CSF, and in spinal cord
motorneurons may be a biological diagnostic index for
ALS. It remains to be directly tested whether restoring
Vgf expressionin spinal cord motor ... sequencing, and the recombinant
virus was packaged by infecting the PacI linearized
recombinant viral DNA into human embryonic kidney
(HEK)-293 cells (Clontech, CA). The resulting recom-
binant...
...
cannot determine the global players involved in such
complex phenotypes. Integration of the gene expres-
sion profiling with specific modulation of gene ex-
pression in relevant signalling pathway ... according the defini-
tions of the Illumina’s (ILL) data as well as of the Il-
lumina’s annotations (table 2).
Int. J. Med. Sci. 2009, 6
http://www.medsci.org
27
weight cargo upon binding ...
Genes detected and in following investigated for valida-
tion
A) an induced expression of the PKN1 gene
B) a repressed expression of the ZCD1 gene
C) a repressed expression of BSG gene...
... protein was released from the chitin column when the chitin-bound intein
tag undergoes self-cleavage in the presence of DTT. Fig 12 showed chitin gel before (N1)
and after (N2) releasing MCoTI-II. ... Aprotinin
4. Re MCoTI-II
Fig 14. Checking the inhibiting activity of
ReMCoTI-II by using diffusion method
1. PA from S. litula
2. PA of S. litula + ReMoTI-II
3. Trypsin
4. Trypsin + ReMCoTI-II ... expression: use LB medium containing 100µg/ml
ampicillin, incubated in shakers at 37
o
C until the OD
600
reached 0.6. Induction by IPTG.
Expression and purification of recombinant MCoTI-II were followed...
... 2009)
doi:10.1111/j.1742-4658.2009.06973.x
Activating enhancer-binding protein (AP)-2e was previously described as a
new regulator of integrin a
10
expression in cartilage. In this study, we ana-
lyzed the expression of AP-2e in differentiated ... the Sox9-binding sites of the
AP-2e promoter (Sox9_1 and Sox9_2). Incubation of
in vitro-synthesized Sox9 with the labeled oligonucleo-
tides containing the Sox9-binding sites resulted in a
strong ... Max-Planck-Institute of Biochemistry, Martinsried, Germany
The family of activating enhancer-binding protein
(AP)-2 transcription factors regulate their target genes
through binding to the palindromic...
... of cytokine
induced expression of intercellular adhesion molecule 1
(ICAM-1) in an intestinal epithelial cell line, a process
blocked by proteasome inhibitors. Gut 42, 779–787.
23 Lakshminarayanan ... to an increase in ICAM-1 expressionin Schwann
cells of sciatic nerves
Aiguo Shen
1,
*, Junling Yang
2,
*, Yangyang Gu
3
, Dan Zhou
4
, Linlin Sun
2
, Yongwei Qin
2
,
Jianping Chen
2
, Ping Wang
2
, ... LPS induces ICAM-1 expression in
SCs, suggest that ICAM-1 may play a role in the focal
accumulation and antigen-induced activation of T cells
in inflammatory demyelinating diseases of the PNS.
As...
... for
silver staining, hot Coomassie s taining, ÔStains-AllÕ labelling
or immunoblotting. T he following running conditions were
used: 60 m in at 1 00 V , 6 0 m in at 500 V, 60 min a t 1000 V,
and ... the
deficiency in dystrophin o n the expression of Ca
2+
-binding
proteins. The relatively unique combination o f the pI value
and molecular mass of individual 2D spots can be useful in
the initial ... carbocyanine dye ÔStains-AllÕ was u sed to
determine potential changes in the expression of major
Ca
2+
-binding proteins in dystrophic fibres. A comparison
between the selective d ye labelling of...
... caveolin proteins [22]. Several studies
have shown that caveolin, a 21–24 kDa integral membrane
protein, plays a critical r ole in the regulation of cell
proliferation, including SMC, by inhibiting ... by inhibiting signalling mol-
ecules [ 45–47]. Caveolin-3 is the main isoform in muscle
cells, including SMC [30]. Caveolin-1 and caveolin-3 seem to
act as scaffolding proteins that negatively ... nine passages a fter primary
culture. Confluent cells were maintained in quiescent mode
by incubation for 24 h in serum-free m edium containing
0.2% (w/v) fatty acid-free BSA. Then, SMC were incubated
or...
... induction of inducible nitric
oxide synthase (iNOS), cyclo-oxygenase-2 (COX-2)
and MMP-9. In this study, we investigated whether
LXR activation inhibits inducible TF expression and
activity in ... finally
degenerating into a lipid core. Plaque rupture exposes
active TF in the lipid core to circulating blood, trigger-
ing thrombosis. As the initiator of coagulation, TF is
a potential target for inhibiting ... the enhanced
expression of in ammatory genes including TF during
the development of atherosclerosis induced by the acti-
vation of NFjB. The current data suggest that LXRs
allow binding of NFjB...
... from both brain and intestine in
L. maderae [14,15].
The finding that N-terminally extended TKRPs are
predominantly expressed only in the gut and not in
the brain are in line with findings of enrichment ... tachykinins, the hemokinins, were identified on
a third precursor encoding gene, preprotachykinin C,
expressed in hematopoietic cells of mouse, rat and
humans [6,7].
In invertebrates the tachykinins ... the insectatachy
kinin ⁄ tachykinin and insect kinin ⁄ Tyr-W-MIF
neuropeptide family pairs. Ann NY Acad Sci 897,
388–400.
2Na
¨
ssel DR (1999) Tachykinin-related peptides in inver-
tebrates: a...
... could also remain trapped and inhibit the
intrinsic uncoupling activity of hUCP3, the digitonin treated
cells were extensively incubated with the resin Dowex-K21.
This extracting procedure was ... times in NaCl/P
i
,thenfixed
in 4% (w/v) paraformaldehyde/NaCl/P
i
for 60 min. The
cells were w ashed four times in NaCl/P
i
and then i ncubated
for 60 min in NaCl/P
i
containing 0.1
M
glycine, ... permeabi-
lized using 0.1% ( v/v) Triton X-100 in NaCl/P
i
for 3 min,
followed by four NaCl/P
i
washes. The slides were then
incubated in blocking buffer containing 5% (v/v) fetal
bovine serum, 0.1%...
... TICK FRDM SKIN; APPLICA-
TIONS TO SKIN OF TURPENTINE, IODINE,
R~'TONE; REMOVAL OF
TICK BY INSERTION
OF NEEDLE BETWEEN MOUTH PARTS; ASPIRIN
FOR PAIN; ANTIBIOTIC TREATMENT IN-
~-~CTIVE. ...
in CMIT, in order of increasing 'enT.ropy'
The enl=ropy
is
given in P.he
first
column;
,':he ent.ries in ~h~ nezt ii columns are
~he percent of occurrences in the 11
disease ... correspond to the disease
categories represented in CMIT. This is done by assign-
ing terms which occur predominantly in one category to a
single vocabulary and then sorting by entropy. Tables...
... believes in
measuring quantitatively something of such an
intrinsically subjective nature as self-
expression in a discipline so detached from
numbers and the right-wrong answer
distinction ... teacher started
assessing them according to the established
marking criteria.
Data analysis: Insights into students’
self -expression during the learning process
Self -expression problems
One ... very active in class discussions:
expressing their wonder at the details of the
text or disagreement with others’ ideas,
defending their opinions convincingly, posing
very interesting questions...
... the
present study indicate that adipophilin protein expressionin muscle is
involved in maintaining insulin sensitivity.
Abbreviations
Adfp, adipophilin; CLB, classical lysis buffer; FA, fatty ... is implicated in
insulin resistance. As shown in the present study,
palmitic acid indeed impaired insulin signaling in
C2C12 cells, which is in line with numerous studies
addressing the effect ... protein expressionin muscle is increased,
accompanied by indications for better insulin sensitiv-
ity. Taken together, the results obtained in the present
study indicate that Adfp expression in...