microarray gene ontology analysis and class distribution of differentially expressed genes

... mean and SEM of (C h and h) and (C 18 h and 24 h) independent experiments with n = 2/group/experiments ***p < 0.001 versus control (NC) (b) The amount of PGE2 released into the media (pg/mg/ml of ... expression of enzymes of prostaglandin biosynthesis and metabolism: 15-hydroxyprostaglandin dehydrogenase Prostaglandins Leukot Essent Fatty Acids 2000, 62:1-5 45 Ivanov AI, Romanovsky AA: Prostaglandin ... encoding COX and PGES isoforms are mechanosensitive or not Materials and methods Prostaglandin E2 (PGE2) is one of the major catabolic mediators involved in cartilage degradation and chondrocyte...

Ngày tải lên: 09/08/2014, 08:22

... control genes, as any variation in the expression of the reference gene between sample groups will reduce the sensitivity of the assay to detect changes in the expression of genes of interest and ... reference genes in QPCR experiments and the reliability of a panel of potential genes should be determined for individual experimental conditions [3,8] Ideally the best two or three of these genes ... reference gene was PP1A with a sumv value of 7.41 and the most reliable gene in of the 10 viral infections Other genes with low sumv values were SDHA (8.91), GAPDH (8.74), TBP (9.06) and EEF1G...

Ngày tải lên: 18/06/2014, 18:20

... selection of the ideal reference gene in gene expression analysis has to be done for each individual experimental setting by evaluat- ing several genes and using the best two or three of these genes ... increasing degree of infection The best genes obtained from all three calculation tools were TBP and PPI TBP seems to be a relative stable expressed gene during the course of virus replication of different ... the BestKeeper tool is shown in table A low standard deviation (SD) of the CT values should be expected for useful reference genes and a high SD for genes that are susceptible to virus replication...

Ngày tải lên: 18/06/2014, 22:20

... control genes, as any variation in the expression of the reference gene between sample groups will reduce the sensitivity of the assay to detect changes in the expression of genes of interest and ... reference genes in QPCR experiments and the reliability of a panel of potential genes should be determined for individual experimental conditions [3,8] Ideally the best two or three of these genes ... reference gene was PP1A with a sumv value of 7.41 and the most reliable gene in of the 10 viral infections Other genes with low sumv values were SDHA (8.91), GAPDH (8.74), TBP (9.06) and EEF1G...

Ngày tải lên: 20/06/2014, 01:20

... selection of the ideal reference gene in gene expression analysis has to be done for each individual experimental setting by evaluat- ing several genes and using the best two or three of these genes ... increasing degree of infection The best genes obtained from all three calculation tools were TBP and PPI TBP seems to be a relative stable expressed gene during the course of virus replication of different ... the BestKeeper tool is shown in table A low standard deviation (SD) of the CT values should be expected for useful reference genes and a high SD for genes that are susceptible to virus replication...

Ngày tải lên: 20/06/2014, 04:20

... set of samples Depending on the expression level of the genes tested, it is suitable to choose a reference gene with similar expression levels of the tested genes Gene expression stability of candidate ... transferred as a Microsoft Excel file (Microsoft, Redmond, WA) for further gene expression stability analysis Analysis of gene expression stability For analysis of gene expression stability and rank, geNorm ... the least stable genes respectively, while there was a slight difference in the order of stability of the other genes The second and third best reference genes were BbrizE1F4A and BbrizEF1 with...

Ngày tải lên: 12/08/2014, 03:20

... the lowest, with a CT of 21.2 In contrast, the highest and lowest expressed genes in V30 were EF1a and ACT, with CTs of 18.3 and 25.1, respectively Analysis of variance of CT values between organs ... effect of including an additional gene [4] If the pairwise variation value for n genes is below a cut-off of 0.15, additional genes are considered not to improve normalization The number of genes ... M values computed by geNorm and qBasePlus allow to rank optimal reference genes For each organ and mix of organs the two top-ranked genes are shown The number of genes required for a reliable...

Ngày tải lên: 12/08/2014, 03:21

... vivo in ducks Results Development and optimization of a TaqMan™ FQ-PCR Final concentrations of primers each of 0.5 μmol/L and probe of 0.25 μmol/L were selected, and the optimized annealing temperature ... combination of primers, probe and annealing temperature was used for subsequent experiments Standard curve establishment The amplification curves (Figure 1.a.) and standard curve (Figure 1.b.) of the ... http://www.virologyj.com/content/7/1/37 Page of 10 Figure The sensitivity of TaqMan™ FQ-PCR detection Ten-fold serial dilutions of AHV-1 standard template were used (1-6), 1.0 × 105-1.0 × 100 copies/reaction of AHV-1 standard template...

Ngày tải lên: 12/08/2014, 04:21

... Figure Gene networks and pathways of 11 genes from PCA analysis The network was analyzed using Ingenuity Pathways Analysis software and is displayed graphically as nodes (genes /gene products) and ... the expression of RAB25 and AMY2A, and positively correlated with TGM2, MALL, and IGSF3 Gene networks and gene pathways To further understand the biological networks of the 19 genes, we next ... using immunohistochemical analysis Results Identification of candidate genes by microarray analysis We selected 100 genes from public DNA microarray data [1-3,6-10] and examined their expression...

Ngày tải lên: 15/02/2014, 04:20

... comprised of multiple subgroup genes (Vb7, 12, 13A, and 17) showed a corresponding number of peaks However, some Vb subgroups comprised of multiple subgroup genes (Vb5, 6, 8, 13B, and 21) showed ... confirmed the existence of 49 functional Vb genes within 24 different Vb subgroups Due to the heterogeneity, some of the 24 Vb subgroups consist of multiple subgroup genes In this study, we designed ... primers annealing to each of 22 different Vb subgroups (36 Vb genes) to quantify expansion of T cell bearing specific Vb subgroups and subgroup genes in response to SAgs One of the important factors...

Ngày tải lên: 18/06/2014, 16:20

... Critical Care 2011, 15:412 http://ccforum.com/content/15/2/412 Page of Figure Cytomegalovirus plasma load measurements during ICU stay of four severe burn patients Table Patient characteristics Patient ... in ICU 133 130,000 Discharged from ICU 105 137,000 Died in ICU ICU stay (days) Viremia peak is expressed in copies/ml DBSA, deep burn surface area; TBSA, total burn surface area ...

Ngày tải lên: 14/08/2014, 07:21

... J, Durland R, Coleman M: Biodistribution and safety studies of hDel-1 plasmidbased gene therapy in mouse and rabbit models Journal of Pharmacy and Pharmacology 2004, 56:177-185 Mena A, Andrew ... Experiment III- analysis of gene expression and distribution of fluorescent complex of pDNA/cationic liposomes Flourescently labelled pDNAX:CDAN/DOPE complexes were prepared and injected i.m ... concentration of saline media, size of plasmid DNA, repeated gene transfer However, neither the influence of lag time between plasmid injection and electroporation nor the effect of the age of mice...

Ngày tải lên: 14/08/2014, 19:22

... identify reference genes for subsequent validation studies on smaller sets of selected genes Key words qPCR, Selection of reference genes, Human candidate reference genes, Candidate reference ... assumption of stable expression of reference genes [2] The average difference in expression level of a gene of interest after normalization with any of two randomly selected nonvalidated reference genes ... stably expressed reference genes This method is robust and independent of cDNA input amount (as long as the same amount is used Reference Genes for RT-qPCR 21 to measure all candidate reference genes...

Ngày tải lên: 05/09/2015, 10:35

... -2, and -3 in the synovium synthase-1, 2, and -3 and (OA) levels of the messages (RA) Relative expressionand rheumatoid arthritis for hyaluronanof knees in 2, and -3 and hyaluronidase-1, -2, and ... HPLC analysis for the measurement of the average molecular weight of HMW hyaluronan Analysis of message expression by quantitative realtime RT-PCR Message expression in the synovium of knees and ... levels of the messages in OA and RA are expressed by longitudinal bars relative to those of the control value expressed as 1.0 The lines outside the bars represent the standard deviation of the...

Ngày tải lên: 09/08/2014, 01:24

... the design of the study FA and WDY drafted the manuscript and participated in the statistical analysis HP and XRH conceived of the study, and participated in its design and coordination and helped ... T: Transcription of cytokeratins 8, 18, and 19 in bone marrow and limited expression of cytokeratins and 20 by carcinoma cells: inherent limitations for RT-PCR in the detection of isolated tumor ... cycle of 4), folfox6 (56 cases, with a median cycle of 6), taxol + cisplatin (4 cases, with a median cycle of 4), taxol + 5FU/ Page of CF (Fluorouracil/Leucovorin, cases, with a median cycle of...

Ngày tải lên: 18/06/2014, 16:20

... differences between HSV-1 and -2 DNA polymerase I genes Archetypal HSV-1 and -2 DNA polymerase I genes share 93% sequence identity and 82% amino acid homology The selection of strain typing pol- ... peak of the correct Tm in dissociation or melting curve analysis plots The accurate estimation of the quantity of HSV was expressed as viral copies/ml of CVL The lower limit of quantitation of ... cycles Standards of 106 to 10 copies per reaction of C trachomatis pgp3 amplicons and negative controls were included in each PCR reaction to generate a standard curve and quantities of the unknown...

Ngày tải lên: 19/06/2014, 08:20

... volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension A) Amplification plots of ... coefficients of variation of Ct values of replicates of standard curve dilutions [21,26] We first examined the fidelity of real-time quantitative PCR in detecting and quantitating JCV T-antigen gene ... each time Copies of JCV DNA in experimental samples were calculated from the standard curve and were expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR...

Ngày tải lên: 19/06/2014, 08:20

... differences between HSV-1 and -2 DNA polymerase I genes Archetypal HSV-1 and -2 DNA polymerase I genes share 93% sequence identity and 82% amino acid homology The selection of strain typing pol- ... peak of the correct Tm in dissociation or melting curve analysis plots The accurate estimation of the quantity of HSV was expressed as viral copies/ml of CVL The lower limit of quantitation of ... cycles Standards of 106 to 10 copies per reaction of C trachomatis pgp3 amplicons and negative controls were included in each PCR reaction to generate a standard curve and quantities of the unknown...

Ngày tải lên: 20/06/2014, 04:20

... volume of 20 µl of PCR mixture Copies of JCV(Mad1) T antigen gene were calculated from the standard curve and were expressed as copies of viral DNA per 100 µl of suspension A) Amplification plots of ... coefficients of variation of Ct values of replicates of standard curve dilutions [21,26] We first examined the fidelity of real-time quantitative PCR in detecting and quantitating JCV T-antigen gene ... each time Copies of JCV DNA in experimental samples were calculated from the standard curve and were expressed as copies of viral DNA per 100 µL of virus suspension The reliability of real-time PCR...

Ngày tải lên: 20/06/2014, 04:20

... Detection limit and standard curve of the multiplex real-time PCR The detection limit and standard curve of the multiplex real-time PCR was determined using S Typhimurium ATCC 14028 and S Enteritidis ... × box analysis [18] in order to compare the results of the standard culture method of isolated field and reference strains with those of the multiplex real-time PCR Results Specificity of the ... Development of a PCR system for the characterisation of Salmonella flagellin genes Acta Vet Hung 2005, 53, 163-172 International Organization for Standardization (ISO) Microbiology of food and animal...

Ngày tải lên: 07/08/2014, 23:22