microarray gene expression profiling of tumours and cell lines

... region of the proximal femur and to compare gene expression in bone from OA, control and OP individuals We identified altered expression of WNT and TGF-β/BMP signalling pathway and target genes ... real-time PCR and consistency between the microarray and PCR detection of expression ratio differences for each of the genes analyzed, suggesting that the majority of the genes identified by the microarray ... fold change in gene expression expressed as ratio of OA to CTL such as MMP25 and S100A4, and downregulated genes such as IBSP, TWIST1 and TIMP4 The altered expression of these genes suggests...

Ngày tải lên: 09/08/2014, 10:21

... cellular localization and cell death (Table 2) and for down-regulated genes included regulation of progression through cell cycle, mitotic phase of cell cycle and DNA damage response and repair (Table ... culture, cells were stained with propidium iodide and analysed by fluorescence activated cell sorting (FACS); a) representative FACS profiles b) the percentage of cells in each phase of the cell ... significant cell death [see Additional File 2] Using these experimental conditions, gene expression data did indeed show changes in levels of genes relevant to cell cycle control Cell death pathway...

Ngày tải lên: 09/08/2014, 09:20

... pyrosequencing data and gene expression profiles of defence-related genes Sequence ID GenBank accession number cDNA Library Max qRT-PCR expression Similarities between 454 and qRTPCR Thaumatin ... 48:575-607 Christoffersen RE, Tucker ML, Laties GG: Cellulase gene expression in ripening avocado fruit: The accumulation of cellulase mRNA and protein as demonstrated by cDNA hybridization and immunodetection ... Cass LG, Kirven KA, Christoffersen RE: Isolation and characterization of a cellulase gene family member expressed during avocado fruit ripening Molecular and General Genetics MGG 1990, 223:76-86...

Ngày tải lên: 11/08/2014, 11:21

... of genes and individual candidates that are involved in the perturbation of mechanisms of iron homeostasis that results from Hfe disruption, and to identify differences in gene expression profiles ... characterized mouse genes, and bioinformatics tools to characterize expression networks in the duodenum and the liver of wild-type control and Hfe-/B6 and D2 mice Results Differential gene expression ... unexpectedly, we observed significant downregulation of the sideroflexin gene (Sfxn2) and upregulation of the mitoferrin gene (Slc25a37) and the Bcrp gene (Abcg2), which encode three molecules that...

Ngày tải lên: 14/08/2014, 08:20

... Traditionally, expression profiling has focused on sampling the tumor cell mass, but this does not take into account the genetic and phenotypic heterogeneity of tumors Moreover, individual genes are identified ... Validation of selected genes by flow cytometry and immunocytochemistry Validation of selected genes by flow cytometry and immunocytochemistry (a) Flow cytometry analysis of prostate cancer cells co-stained ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were...

Ngày tải lên: 14/08/2014, 08:21

... Although somatic cell division is restricted to the A1.0 and A1.5 developmental stages and the two pollen mitoses (first of the initial cell and then of the generative cell to generate the two ... Fernandes J, Walbot V: Comparative profiling of the sense and antisense transcriptome of maize lines Genome Biol 2006, 7:R22 Ma J, Duncan DS, Morrow DJ, Fernandes J, Walbot V: Transcriptome profiling ... Heatmap of relative expression values for genes similar to zinc finger Heatmap of relative expression values for genes similar to zinc finger proteins Genes are sorted by the cluster generated...

Ngày tải lên: 14/08/2014, 21:20

... panel of BL cell lines We next sought to compare quantitative EBV gene transcription profiles in a panel of EBV-harboring BL cell lines The reference cell line B95.8 displays a latency III expression ... profile of EBV gene transcription of a panel of BL cell lines Furthermore, we showed that EBNA1 transcription levels are similar across BL cell lines, suggesting tight transcriptional control of ... Selection of microarray probes Selection of microarray probes The specificity of EBV gene probes was tested in the reference cell line B95.8 as positive control (A) and in the EBV-negative cell line...

Ngày tải lên: 20/06/2014, 01:20

... Quantification and validation of 19 genes by qPCR The expression of 19 genes in test lung cancer cell lines (black and gray) and four validation cell lines (white) was quantified by qPCR and compared ... expression profiling of the 19 genes using four validation cell lines (RERF-LC-MS [AD], LC1/sq [SQ], 86-2 [LC], and MS-1-L [SC]) and the normal control (MRC-9) The results of expression profiling are ... Identification of candidate genes by microarray analysis We selected 100 genes from public DNA microarray data [1-3,6-10] and examined their expression in eight lung cancer cell lines (A549 [AD],...

Ngày tải lên: 15/02/2014, 04:20

... adhesion genes ↑ genes ↓ genes ↑ genes ↓ genes ↑ genes ↓ cell structure genes ↑ 20 genes ↓ 14 genes ↑ 15 genes ↓ 19 genes ↑ 18 genes ↓ metabolism 11 genes ↑ 25 genes ↓ 34 genes ↑ 26 genes ↓ 22 genes ... small cell lung cancer n = 404 41 genes ↑ 56 genes ↑ gene ↓ DNA-repair gene ↓ genes ↑ gene ↓ 14 genes ↑ oncogenes/tumor related genes gene ↑ 11 genes ↓ genes ↑ 11 genes ↓ 13 genes ↑ 10 genes ↓ cell ... ↓ 22 genes ↑ genes ↓ transport genes ↑ 12 genes ↓ 11 genes ↑ 15 genes ↓ genes ↑ 15 genes ↓ development gene ↑ genes ↓ genes ↑ genes ↓ 12 genes ↑ genes ↓ calcium-binding genes ↑ genes ↓ gene ↓...

Ngày tải lên: 18/06/2014, 15:20

... changes in expression of thousands of genes [9,12,13] Another challenge is that the specificity and sensitivity of a given RNA profiling platform are affected by the abundance and variability of the ... spectrophotometry and quality was assessed using an Agilent Bioanalyzer and the Agilent RNA 6000 Nano kit (data not shown) Gene expression profiling Aliquots of each sample were profiled for gene expression ... Gathof BS, Pillai R, Zander T, Schultze JL: Comparison of different isolation techniques prior gene expression profiling of blood derived cells: impact on physiological responses, on overall expression...

Ngày tải lên: 18/06/2014, 16:20

... publication of results achieved with the current state of methodology is essential in the exchange and development of different approaches to gene expression profiling and in comparing selected candidates ... collating of information and development of molecular network models will be essential and will provide the basis for functional interpretation Current status of gene expression profiling in ... limited number of genes Concordance or divergence of results can therefore be estimated only from the selection of genes published in more detail Up to now, gene expression profiling has given...

Ngày tải lên: 09/08/2014, 01:23

... diagnosis of RA, the large variety of cell types (cells of the immune system and of synovial joints) involved in autoimmune arthritic processes, and the extreme genetic heterogeneity of the human ... further analyses Effect of the numbers of replicates on data variability gene expression experiments Average coefficient of variation with increasing number of replicates of gene expression experiments ... phase of arthritis Expression patterns of early arthritis genes R203 Hierarchical clustering of a large number of phase-specific genes (n = 507) (Fig 4) obscured the expression pattern of a relatively...

Ngày tải lên: 09/08/2014, 06:22

... of gene expression and RNA processing data The back end offers a solution to the problem of detecting significant changes in gene expression and RNA processing, while the webbased interface offers ... export of the table into other software, such as R or OpenOffice (or Excel), for automatic conversion of the genes into other IDs (such as Ensembl or Entrez), and for the automatic generation of expression- controlled ... the position of the plus-strand end and the y-axis gives the position of the minus-strand end The colors and sizes of the points indicate the number of reads aligning to each pair of coordinates...

Ngày tải lên: 09/08/2014, 23:20

... two of the twin pairs, two pairs of autistic and unaffected siblings, and a pair of normal monozygotic twins These cell lines had all been used previously for gene expression profiling [21,40] and ... dimensionality of the microarray data and display the overall separation of samples from autistic and control groups Prediction of the potential target genes The lists of the potential target genes of the ... to the differentially expressed genes, which reduced the list of potential gene targets to 94 genes IPA analysis of this set of genes (Table 3) revealed a number of genes significantly involved in...

Ngày tải lên: 11/08/2014, 12:20

... and the gene expression profiles of these cells were compared to the ones of CECs obtained from the same patients before treatment The treatment resulted in differential expression of 2,133 genes ... circulant endothelial cell number and to clarify the molecular effects of the treatment in patients with SSc by studying CECs gene expression Page of 15 profiling before and after the treatment ... transcriptional profiles of CECs obtained from SSc patients Finally, we compared the gene expression profiles of CECs from the two subsets of SSc patients and found that 2,303 genes were significantly...

Ngày tải lên: 12/08/2014, 14:22

... 26:715-727 Cooper S: Cell cycle analysis and microarrays Trends Genet 2002, 18:289-290 Cooper S, Shedden K: Microarray analysis of gene expression during the cell cycle Cell Chromosome 2003, ... experiments in terms of variation in the phase angles of peaking of gene expression in different individual cells Thus, given a particular variation in gene expression in different cells, one can account ... pattern of gene expression during the cell cycle (something, I may add, that I am skeptical of at the start – that is, I am skeptical of whether there are a large number of genes with cell- cycle...

Ngày tải lên: 13/08/2014, 23:20

... the nonrandom co -expression of genes by comparing the number of genes assigned to a specific co -expression profile model to the expected number of genes assigned to the co -expression profile model ... categories of molecular and cellular functions SCS-BTA18-Q Genes — SCS-BTA18-q Control cells SCS-BTA18-q p-values Genes p-values Genes Cellular assembly and organization Cellular function and maintenance ... pathways and genes affecting mastitis susceptibility due Page of 17 to the SCS-BTA18-QTL using a combined approach of holistic gene expression profiling of primary bovine mammary gland epithelial cells...

Ngày tải lên: 14/08/2014, 13:21

... pituitary gland, ovary and oviduct, immediately frozen in liquid nitrogen and stored at -80° C until total RNA extraction Ducks of the second (n = 350) and third (n = 251) generations of white Liancheng ... (http://cn.expasy.org/tools/) Expression profiling To determine the tissue expression of the two type splice variants, semi-quantitative RT-PCR were carried out using total RNA from various duck tissues and a pair of primers ... is the observed value of different egg traits, μ is the population mean; Si and Gj are the fixed effects of each generation and genotype, respectively, and εij is the random error Values are...

Ngày tải lên: 14/08/2014, 13:21

... from precursor cells This process of fat cell development, known as adipogenesis, leads to the accumulation of lipids and an increase in the number and size of fat cells Adipogenesis has been ... lipogenesis and adipogenesis ADD1/SREBP1c can activate a broad program of genes that are involved in fatty acid and triglyceride metabolism in both fat and liver, and can also accelerate adipogenesis ... conducted in the set of 780 ESTs Validation of expression data Four lines of evidence support the quality of our data and its consistency with existing knowledge of fat cell biology First, our...

Ngày tải lên: 14/08/2014, 16:20

... pursued Microarray analysis of gene expression Microarray analysis of gene expression is the parallelization of the traditional northern blots Northern blotting can detect the abundance of a particular ... Induction of stem cells to cardiomyocytes 8! Use of Xenopus laevis in research 9! Microarray analysis of gene expression 13! Gene Ontology 15! Scope of this ... – Standard curves for qPCR profiled genes 92! Figure 3.10 – qPCR expression profiles of selected genes (normalized to ODC) 98! Figure 3.11 – Expression profile correlation with Nkx2-5...

Ngày tải lên: 24/08/2014, 12:29