microarray expression analysis of primary dupuytren s contracture cells

... MCF-7 cells & BCSCs The figure shows one array of the two hybridisations for MCF-7 cells & BCSCs a and b show microarrays for MCF-7 cells, and c and d show microarrays for BCSC cells Table S1 -MiRNAs ... S1 -MiRNAs microarray- based miRNAs expression profile of MCF-7 cells (signal value ≥800) The table shows the miRNAs expression profile of MCF-7 cells obtained through miRNAs microarray Table S2 - ... Table S2 - MiRNAs microarray- based miRNAs expression profile of ESA+CD44+CD24-/low cells (signal value ≥800) The table shows the miRNAs expression profile of ESA +CD44+CD24-/low cells obtained through...

Ngày tải lên: 10/08/2014, 10:20

... donors; B) typical electropherogram of total RNA of human RBCs Microarray analysis of RNA from human RBCs Recent proteomic studies of RBCs based on 1D/2D-electrophoresis 6, or mass spectrometry assay ... anucleate protein synthesis and its meaning in the pathophysiology of diseases Acknowledgements This work was supported by the Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 612 (to ... Georgantas RW3, Tanadve V, Malehorn M et al Microarray and serial analysis of gene expression analyses identify known and novel transcripts overexpressed in hematopoietic stem cells Cancer Res 2004;64:4434-4441...

Ngày tải lên: 03/11/2012, 10:58

... sentences in English and those in Vietnamese Scope of the study This thesis is about contrastive analysis of the primary imperative sentences, one of the three kinds of imperative sentences In this ... make speeches or address the meeting and so on + Expressives: Expressives are speech acts in which the speaker expresses psychological states, feelings or attitudes about a given state of affairs ... first utterance is suggesting, the function of the other one is advising + Commissives: Commissives are speech acts that speakers use to commit themselves to some future action They express what...

Ngày tải lên: 12/12/2013, 00:03

Ngày tải lên: 29/01/2014, 00:23

... from a snapshot of the Internet We call this kind of analysis Static Analysis However, the Internet is a growing network and its topology changes continuously Does the growth mechanism of the ... its robustness? How is the Internet s robustness changing while it is growing? Will performance and robustness of the Internet improve in the future? To answer these questions, we analyze historical ... characteristics of a network are inseparable consequences of its connectivity This linkage is apparent from [11], [12], [13] Scaling factors, such as power-law relationships and Zipf distributions,...

Ngày tải lên: 18/02/2014, 01:20

... kidney, spleen, intestine and gill tissues; however, a more pronounced expression of carp IL-10 was observed in head kidney and intestine tissues A semiquantitative analysis of IL-10 expression ... gene Analysis of IL-10 gene expression in healthy tissues by RT–PCR Total RNA extracted from the cell suspension of organs isolated from healthy carp were used for cDNA synthesis by ReverTra Dash ... Bonilla, S. , Cunningham, C & Secombes, C.J (2001) Cloning and sequencing of caspase in rainbow trout, Oncorhynchus mykiss, and analysis of its expression under conditions known to induce apoptosis Dev...

Ngày tải lên: 20/02/2014, 02:21

... excite sensations; 4, the relations of Succession and Coexistence, Likeness and Unlikeness, which subsist really only between states of consciousness These four classes are a substitute for Aristotle 's ... rests, in the last resort, on this process But as this is itself syllogistic, it is useless, as a proof of a syllogism, against a man who denies the validity of this kind of reasoning process ... recipient both of the sensations and of all the other feelings Though I call a something myself, as distinct from the series of feelings, the 'thread of consciousness,' yet this self shows itself only...

Ngày tải lên: 07/03/2014, 03:20

... pastoris A glucose-mannosebinding legume lectin from the seeds of Canavalia Expression of nucleocytoplasmic Orysata brasiliensis, a homolog of the classical vacuolar concanavalin A, was also expressed ... heterologous expression of Orysata, a JRL from rice Based on a detailed analysis of its sequence, this lectin was predicted to locate to the nucleocytoplasmic compartment of plant cells, as shown by expression ... expression of a fusion protein in tobacco cells Furthermore, the successful expression of the His-tagged Orysata in the yeast P pastoris allowed sufficient amounts of the lectin to be purified to study...

Ngày tải lên: 14/03/2014, 23:20

... expression whilst inhibiting IL-18A expression In mouse, IL-18 expression is controlled by two promoters, one responsible for constitutive expression and one for inducible expression [33] It is ... presented are for a representative experiment of three fish examined Expression studies To determine the tissue distribution of IL-18 expression, RT-PCR was performed using two pairs of primers, ... Direct comparison of two sequences was performed using the GAP analysis program within the Wisconsin Genetics Computer Group Sequence Analysis Software Package (version 10.0) [43] Signal leader...

Ngày tải lên: 16/03/2014, 16:20

... chose this time window because a live-broadcast primary debate usually lasts about two hours The bottomleft shows system statistics, including the total number of tweets, the number of seconds since ... Singapore s 2011 presidential election Past studies of political sentiment on social networks have been either post-hoc and/or carried out on small and static samples To address these issues, we built ... repetition of symbols and Unicode characters (see Figure for an example) 3.3 Sentiment Model The design of the sentiment model used in our system was based on the assumption that the opinions expressed...

Ngày tải lên: 16/03/2014, 20:20

... these domains and that there is a high selective pressure to maintain the arrangement of these exons In mammals, hypoxic stress is known to increase GLUT1 and GLUT3 expression in specific tissues ... searches were performed using the online BLAST suite of programs (NCBI, USA) Statistical analysis A nonparametric v2 test was used to test the hypothesis that the ratio of expression level in the hypoxic ... expression pattern of fish GLUTs in response to hypoxic stress Here, we have demonstrated for the first time a hypoxiaresponsive GLUT gene that is both most prominently expressed and responsive...

Ngày tải lên: 17/03/2014, 03:20

... audience until the end of the film, is one of neurosis versus psychosis The compulsive and obsessive actions that drove Marion to steal the money is recognisable, albeit unusual behaviour, that ... the introduction of Sam, Lila, and Arbogast Hitchcock uses Arbogast 's character to arouse suspicion within the audience Arbogast 's murder is not as intense as Marion 's because the audience had ... decision and continues with her journey The split personality motif reaches the height of its foreshadowing power as Marion battles both sides of her conscience while driving on an ominous and seemingly...

Ngày tải lên: 21/03/2014, 22:10

... Genomic structure and expression analysis of the Cc RNase Results Expression analysis of Cc RNase In our previous work, we reported the cloning and overexpression of a cDNA sequence of 864 bp ... and expression analysis of the Cc RNase A B FEBS Journal 275 (2008) 6217–6227 ª 2008 The Authors Journal compilation ª 2008 FEBS 6223 Genomic structure and expression analysis of the Cc RNase ... transcript (AAUAUA) is slightly different from the consensus signal that is used for formation of the longer transcript, it is possible that these transcripts are expressed at different levels...

Ngày tải lên: 23/03/2014, 06:20

... negative control Histochemical analysis of GUS activity Histochemical GUS staining was performed for T2 vegetative tissues (leaves, roots, stems, siliques, seeds, inflorescences) and pollen with ... elements found within the identified regulatory regions of the genes is discussed Finally, an evolutionary relationship based on sequence comparison of these proteases is presented Results Isolation ... expression is mainly restricted to the flowers, although staining can also be observed in young stems At the initial stages of pistil development, intense staining is observed in the stigma, style...

Ngày tải lên: 30/03/2014, 09:20

... lines by in vitro GCSF treatment Summing up, our results show significant expression of CD46 on various types of blood leukocytes including hematopoietic progenitor cells Consequently these cells ... infection [12] Recently, analysis of the short consensus repeat (SCR) regions that comprise most of the extracellular domain of CD46, was shown to have an essential role of SCR and in HHV-6 receptor ... progenitor cells from cord blood (CB) [squares], peripheral blood of adult donors (PB) [circles], leukapheresis products (LP) from G-CSF mobilised precursor cells [triangles] and MACS sorted CB CD34+ cells...

Ngày tải lên: 20/06/2014, 01:20

... the present article, we report two cases of mental disorders as clinical presentations of primary SS, suggesting that psychiatrists should keep in mind that SS can be a potential cause of psychiatric ... the psychiatric disorder In general, this consideration holds for all autoimmune diseases Psychiatric presentation of primary SS also suggests that mental disorders not occur only as a response ... patients without any potentially associated disease, primary SS may be defined as follows: (a) The presence of any four of the six items is indicative of primary SS, as long as either item (Histopathology)...

Ngày tải lên: 08/08/2014, 23:21

... gene expression analysis of active disease Statistical analysis of the difference of gene expression in samples from patients and 12 healthy controls gave the following results 1716 genes of the ... samples (expression levels > SD over background as defined above) were used for this analysis statistically differentially expressed in either patients or controls These genes passed the Student s t-test ... This analysis is based on the observation that the expression levels of the majority of genes not change in compared samples, and that expression values are normally distributed around a regression...

Ngày tải lên: 09/08/2014, 01:23

... control synovium (250% of the control value) (Fig 1) Expression profile of hyaluronidase isozyme messages Statistical analysis Statistical analysis was with Wilcoxon 's matched-pairs signed rank test ... in the synovial fluid of OA or RA patients were significantly lower than those of control donors (Table 3) Expression profile of hyaluronan synthase isoform messages Expressed messages for all ... HAS isoforms were detected in all synovial samples The expression of the messages for HAS-1 and HAS-2 was significantly less in the synovium of OA than in the control synovium (83% and 48% of...

Ngày tải lên: 09/08/2014, 01:24

... However, assume that sample A also contains a set of genes equal in number and expression that are not expressed in B Thus, sample A has twice as many total expressed genes as sample B, that is, its ... downstream statistical analysis procedures, thus accounting for the sampling properties of RNA-seq data Results and discussion A hypothetical scenario Estimated normalization factors should ensure ... gives an estimated TMM scaling factor of 1.04 between the two samples (embryoid bodies versus embryonic stem cells) , sequenced on the SOLiD™ system The M versus A plot for this dataset also highlights...

Ngày tải lên: 09/08/2014, 20:21

... biosynthesis/degradation genes EST BLAST search result Number of haplotypes SNPs InDels Approx sequence length (bp) HY09D18 Sucrose synthase 18 HF08A21 Sucrose synthase 14 550 HA31O14 Sucrose synthase ... R-based expression microarray quality assessment and analysis Plant Physiol 2010, 153:642-651 54 Sturn A, Quackenbush J, Trajanoski Z: Genesis: cluster analysis of microarray data Bioinformatics ... Contig460 _s_ at: sucrose synthase GBS3258: Contig481_at: sucrose synthase STn10: Contig481 _s_ at: sucrose synthase STn02: Contig823_at: sucrose synthase GBS3259: Contig3390_at: ADP-glucose pyrophosphorylase...

Ngày tải lên: 11/08/2014, 11:21