methyl d aspartate receptor antibodies
Effects of apolipoprotein e on n methyl d aspartate receptor signalling the roles of ageing and chicken extract
Ngày tải lên :
30/09/2015, 06:42
... Final volume was standardized by adding PBS and 6x loading dye containing β-mercaptoethanol were added The mixtures were heated at 95°C for minutes and loaded onto a hand-cast 7.5 or 10% Tris-glycine ... human AD brain, there is reduced NR1 in frontal cortex and hippocampus but not amygdala (Amada et al., 2005); and reduced NR2A and NR2B (Bi and Sze, 2002) in hippocampus whereas NR2C and NR 2D not ... plasticity towards LTD formation 27 Chapter 2: Materials And Methods Chapter 2: Materials And Methods 2.1 Common reagents and materials Dulbecco’s Modified Eagles Medium (DMEM) culture medium (Gibco)...
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Mechanism of hydrogen sulphide mediated signaling cascade through n methyl d aspartate receptors
Ngày tải lên :
10/11/2015, 12:35
... 3.2.4 Dose-dependent decrease in cell viability of NMDA-treated mature day neurons 58 Figure 3.2.5 NMDA and NaHS-treated neurons demonstrated significant cleavage of alpha-fodrin to 145/150 kDa ... Sodium Hydrosulfide MTT: 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide NB: NeuroBasal medium NMDA: N -methyl- D- Aspartate NO: Nitric Oxide PCD: Programmed Cell Death PCR: Polymerase ... receptors and sodium hydrosulphide (NaHS) as a H2S donor, H2S is demonstrated to induce apoptotic-necrotic continuum in a dose- and timedependent manner via activation of calcium-dependent proteases,...
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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx
Ngày tải lên :
22/02/2014, 07:20
... NADPþ, mM NADPH, mM ATP, 20 mM FAD and chromoplasts or chromoplast extract Isotope-labeled 2C -methyl- D -erythritol 4-phosphate and/or 2C -methyl- D -erythritol 2,4-cyclodiphosphate were added as ... g) were homogenized and lyophilized The dry powder was extracted with ethyl acetate (2.5 L) The solution was brought to dryness under reduced pressure The residue was dissolved in 10 mL of a Aliquots ... measured in CDCl3, and 2C -methyl- D -erythritol 2,4-cyclodiphosphate was measured in D2 O R E S U LT S Isolated chromoplasts of C annuum were incubated with mixtures of [1-3H]2C -methyl- D -erythritol...
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Báo cáo khoa học: Biosynthesis of isoprenoids – studies on the mechanism of 2C-methyl-D-erythritol-4-phosphate synthase pdf
Ngày tải lên :
16/03/2014, 06:20
... of 1-deoxy -D- xylulose 5-phosphate and its diastereomer Spectra were recorded in time intervals of days at 37 °C (A) Day (B) Day (C) Day (D) Day (E) Day The signals of [3,4,5-13C3]1-deoxy -D- xylulose ... analyzed by TLC and 13C-NMR spectroscopy, and were then combined and evaporated to a small volume under reduced pressure The solution was lyophilized The residue was dissolved in H2O and stored at ... protein was removed by ultrafiltration (3 kDa cutoff) The solution was lyophilized The residue was dissolved in mL of water, and the pH was adjusted to Sodium metaperiodate was added to a final concentration...
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Báo cáo khoa học: Cloning and functional characterization of Arabidopsis thaliana D-amino acid aminotransferase – D-aspartate behavior during germination pdf
Ngày tải lên :
30/03/2014, 04:20
... the A thaliana d- AAT D- Ala D- Asp D- Gln D- Asn D- Cys D- Met D- Thr D- Ser D- His D- Arg D- Lys D- Leu D- Val D- Tyr D- Phe D- Trp D- Pro 20 40 60 80 100 120 Relative activity (%) Fig D- Amino acid substrate specificity ... other d- amino acids metabolically related to d- Asp, in particular d- Glu and d- Ala, were determined Plants cultured in MS medium, in the absence of d- Asp, showed no detectable levels of d- Glu or d- Ala ... good agreement with that calculated from its deduced amino acid sequence The d- AAT preparation, purified as described in Experimental procedures, exhibited considerable AT activity for d- Asp and...
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Báo cáo khoa học: Caenorhabditis elegans has two genes encoding functional D-aspartate oxidases pot
Ngày tải lên :
30/03/2014, 10:20
... 1.72 1.22 0.11 ND ND ND 2113 4082 15 394 ND ND ND ND ND ND 4.29 5.31 6.13 ND ND ND 2.02 0.23 0.84 ND ND ND 2125 23 066 7300 ND ND ND 3.07 2.11 3.00 ND ND ND 0.38 0.25 1.84 ND ND ND 8066 8427 1629 ... assayed by using purified Y69Ap (A), C47Ap (B), and F18Ep (C) The substrates used were D- Ala (s), D- Met (h), and D- Arg (n) for Y69Ap, and D- Asp (d) , D- Glu (j), and NMDA (m) for C47Ap and F18Ep ... independent assays ND, Not determined; NMLA, N -methyl- L -aspartate Relative activity (%) Substrate Y69Ap D- Ala 100 ± 172 ± 19 ± 55 ± 2.2 ± < 0.1 < 0.1 34 ± 1.9 ± < 0.1 ND ND ND D- Met D- Asn D- Arg D- Asp...
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Báo cáo khoa học: Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection pot
Ngày tải lên :
30/03/2014, 20:20
... D R G RPS S T N T RSS EDNQRPS-EDNQRPS EDNQRPS 0123456ab VL CDR2 GIGG SGDTTYYADSVKG GIGG SGDTTYYADSVKG GISY DGYNKYY GDSVKG GISY DGYNKYY GDSVKG GISY DGYNKYY ADSVKG 012abc3456789012345 VH CDR2 ... RFTISRDN S KNTVDLQ I N N LR P EDT A V YYCAR RFTISRDN P KNTVDLQ M N S LR A EDT S M YYCAR RFTISRDN P KNTVDLQ M N S LR A EDT S M YYCAR RFTISRDNSKSTLYLQMNSL G ADDTAIYYCAK RFTISRDNSKSTLYLQMNSL R ADDTAIYYCAK ... were washed and HRP–streptavidin was added The mixture was incubated for h at room temperature and then treated with biotinylated tyramine as described above The beads were used to directly infect...
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Báo cáo y học: "Characterization of a panel of six β2-adrenergic receptor antibodies by indirect immunofluorescence microscopy" doc
Ngày tải lên :
12/08/2014, 15:21
... α-actin antibodies (A) C – merged image Airway epithelial cells were harvested from rat lungs, fixed and double-labeled with β2AR (Ab-13989, E and Ab-sc570, H) and E-cadherin antibodies (D, G, and ... anticipated that this antibody would recognize both the rat and human receptors Indeed, when tested in transfected HEK 293 cells, Ab-13989 recognized both rat and human β2AR (Figure 3) Table 1: Antibodies ... labeled Avidin D (Vector Laboratories Inc., Burlingame, CA) Optimal dilutions of the antibodies were determined in titration experiments Antibodies were diluted in the permeabilization buffer and...
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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf
Ngày tải lên :
19/02/2014, 02:20
... increased two-fold in endosomes isolated from CT-injected rats (closed squares), but this was not observed for CT-B-injected (closed diamonds) or diptheria toxin-injected rats (closed circles) ... kDa fragment was consistently observed in endosomal hydrolysates of CT obtained at acidic pH, and was strictly cathepsin D- dependent; its detection coincided with 32P-labeling of Gsa by CT hydrolysates ... chloroquine and monensin interfered with the ATP-dependent intraendosomal degradation of internalized radioactive CT [6] Recently, we have identified an endosomal aspartic acid protease, cathepsin D, that...
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Báo cáo Y học: Variations in receptor site-3 on rat brain and insect sodium channels highlighted by binding of a funnel-web spider d-atracotoxin pdf
Ngày tải lên :
08/03/2014, 16:20
... (see Experimental procedures) identified an iodinated Tyr22 in the first peak and an iodinated Tyr25 in the second peak Both iodinated derivatives did not differ in their binding properties to cockroach ... cockroach sodium channels [36], we identified which Tyr residue was iodinated on 125I-labelled d- ACTX-Hv1a Amino acid sequence analysis of the two radiolabelled peaks obtained during toxin radioiodination ... ligand in association kinetic determinations was adjusted to keep the reaction at pseudo-first order conditions and varied according to the Kd values of the toxin under polarized or depolarized conditions...
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Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf
Ngày tải lên :
23/03/2014, 04:21
... Calculated using MOLEd MAN [48] A stringent-boundary Ramachandran plot was used [49] b and 257–293; domain includes residues 111–256 and 295–307 In each molecule, structural sodium and calcium ... domains (Fig 1) As illustrated in Fig 2, 15 hydrogen bonds directly link protein and ligand, six of which arise from domain 1, and nine from domain Two water molecules also make hydrogen bonds ... sequence (annotated as a RbsB), but replaced by other residues in the authentic ribose-binding proteins In addition, the repressor sequences include a DNA-binding headpiece, and so are consistently...
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báo cáo hóa học: " Methyl salicylate 2-O-b-D-lactoside, a novel salicylic acid analogue, acts as an antiinflammatory agent on microglia and astrocytes" docx
Ngày tải lên :
19/06/2014, 22:20
... Medium was then removed, and DL0309 (0.1, 1.0 or 10 μM) was added for h Cells were treated with arachidonic acid, 30 μM, for another 20 min, and PGE2 levels in the medium were then measured by ... markedly increased (about 8-fold) NO production in the cells, compared to that generated in control cells; DL0309 inhibited LPS-induced NO release in microglia and astrocytes, both in a dose-dependent ... Pre-treatment with DL0309 reduced this protein expression in a dose-dependent manner in microglia Additionally, DL0309 also directly inhibited total COX (COX-1/2, Figure 4C) and COX-1 (Figure 4D) activity...
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Báo cáo y học: "Association between vitamin D receptor gene haplotypes and chronic periodontitis among Japanese men" doc
Ngày tải lên :
08/08/2014, 16:23
... considered, these effects were not noticed [10] Clearly, additional studies are needed to determine whether the combined VDR polymorphisms influence the risk of developing periodontitis under ... needed Accumulated evidence indicates that diabetes is a major risk factor for periodontitis, and severe 221 periodontitis often coexists with diabetes [40] Taylor et al [41] concluded that diabetics ... haplotype and periodontal disease that we observed was independent of current smoking status and the prevalence of diabetes as a statistical adjustment did not diminish the influence of the VDR haplotype...
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Báo cáo y học: "A functional variant of Fcγ receptor IIIA is associated with rheumatoid arthritis in individuals who are positive for anti-glucose-6-phosphate isomerase antibodies" doc
Ngày tải lên :
09/08/2014, 07:20
... FCGR3A and FCGR2A genes cDNA was genes amplified with primers and restriction digested using appropriate enzymes Digested PCR products were visualized with ethidium bromide (a) FCGR3A gene and (b) ... FCGR2A gene ND, nondigested PCR product; RE, restriction enzyme Population of anti-GPI FCGR2A genotypes antibody positive individuals, and FCGR3A and FCGR2A genotypes The study included 187 patients ... healthy individuals In individuals homozygous for the FCGR3A high affinity V/V genotype (14 RA patients and healthy individuals), both anti-human GPI antibodies and anti-rabbit GPI antibodies were...
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Báo cáo y học: "Vitamin D receptor gene polymorphisms and susceptibility of hand osteoarthritis in Finnish wome" pdf
Ngày tải lên :
09/08/2014, 07:20
... association was observed between individual VDR gene polymorphisms and the overall odds of hand OA However, the VDR aa genotype posed an almost doubled odds of symmetrical hand OA (OR = 1.93, 95% ... participated in the evaluation of reliability of radiographs' readings HR conceived of the study and participated in its design PLA conceived of the study and participated in its design and coordination ... Ann Rheum Dis 1989, 48:271-280 53 Sowers M, Lachance L, Hochberg M, Jamadar D: Radiographically defined osteoarthritis of the hand and knee in young and middle-aged African American and Caucasian...
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Báo cáo y học: "Vitamin D receptor gene BsmI polymorphisms in Thai patients with systemic lupus erythematosus" potx
Ngày tải lên :
09/08/2014, 07:20
... Materials and methods This study was conducted in accordance with the principles embodied in the Declaration of Helsinki and was approved by the ethical committees of the Ramathibodi Hospital, Mahidol ... statistically demonstrated (Table 3) Dicussion Most tissues in the body, including heart, stomach, pancreas, bone, skin, gonads, and activated T and B lymphocytes, have the nuclear receptor for 1,25-dihydroxyvitamin ... design, coordination and acquisition of data OV carried out the molecular genetic study and performed statistical analysis KN and KT participated in coordination and interpretation of data PL participated...
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Báo cáo y học: " Automated ERCC1 immunochemistry on hybrid cytology/tissue microarray of malignant effusions: evaluation of antibodies 8F1 and D-10." potx
Ngày tải lên :
10/08/2014, 09:22
... of 36 kD was observed on immunoblot using 8F1 and this band disappeared after siRNAmediated depletion [11] In this study, both 8F1 and D- 10 homogeneously and robustly stained the whole nuclear ... for many different antibodies Bioinformatics research is ongoing to generate software tools for automated analysis of TMA localization data and XLM-based standardized data capture and transfer ... confidently detected His-tagged purified ERCC1 In reply, the authors of the first NSCLC study demonstrated that in the HeLa and the A549 lung adenocarcinoma cell lines, one major band of 36 kD was...
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Báo cáo khoa hoc:" Vitamin D receptor gene polymorphisms in multiple sclerosis patients in northwest Greece" docx
Ngày tải lên :
11/08/2014, 07:21
... control individuals matched for sex and age All patients satisfied the Mc Donald criteria for MS diagnosis [17] None of the case and control individuals were receiving chronically steroids, bisphosphonates ... excluded from the study Patients and controls were asked to give 10 ml of blood for the study and they signed informed consent for testing The research protocol and consent form was approved by ... performed to examine factors independently associated with the Taq-I and Bsm-I polymorphisms of the VDR gene Gender, age, body mass index (BMI), MS diagnosis versus controls, smoking duration and lumbar...
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Báo cáo khoa hoc:" Vitamin D and oestrogen receptor polymorphisms in developmental dysplasia of the hip and primary protrusio acetabuli – A preliminary study" ppt
Ngày tải lên :
11/08/2014, 08:21
... respective signal transduction pathways Several interactions between the vitamin D and oestrogen endocrine system have been described 1,25-Dihydroxyvitamin D3 (1,25-(OH) 2D3 ) and 17β-estradiol (E2) have ... biosynthesis [19,20] and receptor We recruited 45 patient with DDH and 20 patients with PPA In all patients, a diagnosis of DDH or PPA was made on the basis of clinical and radiographic examination ... results validated by an independent, blinded observer examining the agarose gels (AF) 10% of the assays were repeated and analysed by an independent observer The PCR assays were performed with at...
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Báo cáo y học: " Severe hydrops in the infant of a Rhesus D-positive mother due to anti-c antibodies diagnosed antenatally: a case report" pot
Ngày tải lên :
11/08/2014, 11:23
... leading to a suspicion of the presence of non-anti -D antibodies A special request was sent to the blood bank to screen for uncommon rare blood groups and non -D antibodies Anti-c was detected in ... conjugated hyperbilirubinemia due to inspissated bile syndrome, which resolved on its own The baby was then managed conservatively and was discharged in stable condition on the 30th day of her ... In various retrospective studies, haemolytic disease of the newborn has been reported due to Anti-c antibodies [9-11] The frequency of D and Non -D antigens differ in different populations with...
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