methods for the detection of waterborne viruses

... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods for the Detection of Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... Comparison of Diagnostic Methods for the Detection of Parasites in Fish al., 1998; Anderson, 1999) Our primer sets were designed for identification of different flukes and they were useful for detection...

Ngày tải lên: 06/08/2014, 19:20

... virus detection rates The number of influenza-positive nasal swabs and the CT values were similar in the samples collected by the pediatrician and parents Furthermore, the direct involvement of ... four There were no differences in CT values suggesting less virus and lower sensitivity, and no differences in the timing of the collections or in the age or weight of the children In any case, the ... detection rates were similar in the samples collected using the two methods (Cohen's kappa = 0.86): in comparison with the pediatricians, the sensitivity and specificity of the parental collections...

Ngày tải lên: 12/08/2014, 04:20

... where the location of the unmapped read may span the breakpoint of the CNV SR analysis has the advantage of being able to pinpoint the location of the breakpoints Assembly-based AS methods, on the ... STATISTICAL METHODS FOR THE DETECTION AND ANALYSES OF STRUCTURAL VARIANTS IN THE HUMAN GENOME TEO SHU MEI A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS GRADUATE SCHOOL FOR INTEGRATIVE ... using these technologies are unfortunately limited by the density of the probes, as well as the location of the probes For example, array platforms with more than million probes have a lower detection...

Ngày tải lên: 09/09/2015, 10:14

... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting ... Moreover, to assess the performance of the prediction of stenosis independent of the prevalence of stenosis, the positive and negative likelihood ratios (LR) were calculated [24] A value of p

Ngày tải lên: 03/11/2012, 10:58

... supported by the research project The research on the development of the total evaluation technique for the hazardous impacts by the chemical substances towards human and ecology (the project ... and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated rat hepatocytes treated with 6-nitrochrysene, ... different methods (gas chromatograph-mass spectrometry, 32P postlabeling method, etc.) Therefore, it is important to develop a simple rapid in vitro method for evaluating the generation of reactive...

Ngày tải lên: 05/09/2013, 08:40

... of 95 ml) The A280 to A260 ratio of the UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide ... compared to the other methods The SDS-extracted polymer included a small amount of material other than protein Furthermore, the steaming- and washing-extracted polymers contained large amounts of phosphorus ... buffer at a flow rate of ml/h and ml fractions were collected The void volume of the column was determined by the elution of Blue Dextran (Pharmacia) Other analyses - 147 - Journal of Water and Environment...

Ngày tải lên: 05/09/2013, 09:08

... respectively One of the weaknesses of this study is the small sample size In addition, the majority of the lesions examined appear to be of the Class I type, rather than Class II lesions for which the technology ... appropriate to the purpose of the test? Use examiners representative of the target or primary clinical providers of the test? Show evidence that the test can distinguish cancer/precancer from other conditions ... predictive value Therefore, the lack of apparent control as well as documentation with respect to the manner in which the sample was selected limits the utility of this study Poate et al performed a...

Ngày tải lên: 06/03/2014, 02:21

... drops of the creatine solution, three drops of the ethanolic solution of 1-naphthol and then two drops of the potassium hydroxide solution; shake after the addition of each reagent The formation of ... incubation for 24 h ± h, examine the plates (9.4.3) for the presence of typical colonies of Salmonella and atypical colonies that may be Salmonella (see Note) Mark their position on the bottom of the ... the results of the interpretation, indicate the presence or absence of Salmonella in a test portion of x g or x ml of product (see ISO 7218) See annex C for the precision data obtained from the...

Ngày tải lên: 07/03/2014, 16:20

... the concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the literature [18] ... one of the roles of promoter may be to provide a surface for the catalytic conversion of NO to NO2 that are responsible for the high sensitivity associated with the promotion However, whether ... and was then diffracted onto the detector The detector collected data for all angles in the range 4±648, 2y, simultaneously Fig gives the diffraction spectra of the WO3 before and after the NO...

Ngày tải lên: 19/03/2014, 16:47

... scrutinized the German version of this text Prof Nirschl suggested the idea of this book Prof Reimert organized the continuation of the research unit after the DFG funding as well as Prof Kasper, Prof ... derivation due to the time-dependence of the unit vectors Using the product rule for the derivation and the fact that the time derivative of the unit vector is the cross product of !rf with this ... the scaling of the argument on index k of the measured data in reciprocal space As the sum is finite, it only represents an approximation of the delta function The position of the origin and the...

Ngày tải lên: 29/05/2014, 18:32

... those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses ... sequence information for MneRV2 consisted only of a region of the DNA polymerase which had significant sequence similarity with the macaque RV1 rhadinoviruses, and therefore was unsuitable for the desired ... quantitate the viral load of macaque rhadinoviruses belonging to the RV2 lineage of KSHV-like rhadinoviruses The primers and probe for this assay were based on sequences within the 3' end of the ORF...

Ngày tải lên: 18/06/2014, 22:20

... knowledge related to the mechanics of human movement The work at the University of California formed the basis for many of the fundamental techniques currently used for the study of human locomotion ... patterns of locomotion requires new methods that would limit the risk of producing artifact due to markers or the constraints of the testing methods For example, the constraints of the laboratory ... limiting the advancement of the study of human movement is the measurement of skeletal movement from markers or sensors placed on the skin The movement of the markers is typically used to infer the...

Ngày tải lên: 19/06/2014, 10:20

... those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses ... sequence information for MneRV2 consisted only of a region of the DNA polymerase which had significant sequence similarity with the macaque RV1 rhadinoviruses, and therefore was unsuitable for the desired ... quantitate the viral load of macaque rhadinoviruses belonging to the RV2 lineage of KSHV-like rhadinoviruses The primers and probe for this assay were based on sequences within the 3' end of the ORF...

Ngày tải lên: 20/06/2014, 04:20

... differentiated hMSCs, also in the corner areas of the microcavity (Figure 3f) The performed studies verify the biocompatibility of the Si3N4 membrane and the suitability of the microcavity for in vitro studies ... Parkinson disease, for the development of new sensitive drug screening methods and for the quantification of toxicodynamic and toxicokinetic effects Application of the MCC for the analysis of nanoparticleinduced ... exposures Therefore, the analysis and quantification of single-cell responses will provide important information on the toxicity of the tested materials The MCC-based method is therefore qualified...

Ngày tải lên: 21/06/2014, 00:20

... (12) then corresponds to the correlation of the real part, z1c(nT), of the SMF’s output, y1c(nT), with the known real symbols, an, over the known signal duration B Performance The performance of ... detectors as the phase diversity between the sources increases We still note the best performance of the completely informed detectors (C1 and O1) and the lowest performance of the less informed detectors ... statistical theory of detection, the optimal receiver for the detection of the K symbols an from both the K primary data xv(nT) and the K’ secondary data bTv(nT)’ consists in comparing to a threshold the...

Ngày tải lên: 21/06/2014, 00:20

... Page of adjust the pH of the mixture to 9.1 under stirring The NaHTe solution prepared in the first step was added to the reaction mixture under N protection The resultant mixture was stirred for ... cutoff value in succedent detection was determined as a positive result The ten HPV-16-positive samples were used to validate our hybridization assay on the basis of the cutoff value Detection of ... Page of predenatured at 95°C for 10 min, then 55°C for 30 The particles coupled with streptavidin were added into the hybridization mixtures and incubated at 37°C for 10 and enriched in the bottom...

Ngày tải lên: 21/06/2014, 01:20

... on the percentage of fully brown beans in the cut test 6: The effect of spreading of beans for two hours, prior to placement in boxes, on fermentation temperatures 7: The effect of spreading of ... reveals the presence of certain defects which may cause off-flavours and indicates the degree of fermentation of the beans which has a bearing on the flavour and quality of the beans The International ... case of larger quantities of beans e.g 250kg, partitioned boxes (Illustration 13) are used In this case, turning of beans is performed by shovelling them from one side of the partition to the other...

Ngày tải lên: 21/06/2014, 06:20

... probability of detection close to 85% could be achieved for a probability of false alarm 10% in the case of vocal fold paralysis in male utterances, while the probability of detection for vocal ... probability of detection for vocal fold paralysis in men was measured, that is approximately equal to 90% for probability of false alarm 10% The gain in the probability of detection for vocal fold ... probability of detection approximately 73% for probability of false alarm 4% [33] For the same probability of false alarm, a probability of detection, which falls between 80.95% for rectangular...

Ngày tải lên: 21/06/2014, 20:20

... selectivity when they are used just for Zn(II) detection On the other hand, the fluorescence enhancement of MWNTs-GNQ is only for Zn(II) As the use of organic–inorganic hybrid materials for bio-application ... intracellular environment for the detection of Zn(II) References ð1Þ Wherein, A is the value of the fluorescence intensity, C is the concentration of Zn(II), and the range of C is from 0.2 lM to ... happened in the temperature range from 200 °C to 450 °C due to the degradation of the GNQ grafted to the MWNTs The content of the GNQ grafted to the MWNTs is about 12 wt%, which is similar to the calculation...

Ngày tải lên: 22/06/2014, 01:20

... difj,max ferent methods: the center -of- mass and the Blais-and-Rioux operators The first uses the distribution of the grey level to retrieve the positions of the maxima, whereas the second applies ... of a widely used real-time industrial application The core component of the algorithm is the selection and the extraction of the features best describing the image contents The first step of the ... illuminations must be used for covering the complete circumference of a metallic cylinder The number NC of needed cameras depends not only on the diameter of the object DO , the width of the ray aperture’s...

Ngày tải lên: 22/06/2014, 19:20