methods for the detection of ros in human sperm samples

... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... compound microscope Stretching of these metacercariae was done by hot formalin for two minutes Then, they were kept in Eppendorf tubes with neutral formalin (4%) Diagnosis of parasitic infections ... saline pH 7.0) The internal organs were exposed after a vertical incision was made from the anal opening to the Before any dissection, the exterior of the fish was observed under the dissecting...

Ngày tải lên: 06/08/2014, 19:20

... ta nim 03 rof detabucni erew setalp eht ,yltneuqesbuS llew hcae ot dedda saw noitulos etartsbus )]dica cinoflus enilozaihtzneb lyhte-3[id-oniza-2,2( STBA lµ 001 ,gnihsaw retfA h rof esadixorep ... syad evitucesnoc rof yad/gk/gm 02 ta stibbar ot ylralucsumartni deretsinimda saw nicymanaK nicymanak fo noitartsinimda ralucsumartni retfa amsalp tibbar ni nicymanak fo eliforp noitelpeD elbaT ... etis-A eht ni egnahc lanoitamrofnoc lacol a secudni gnidnib nicymomoraP DJ isilguP ,S awazihsoY ,D ymruoF 054-344 ,36 ,9791 scirtaideP yduts pu-wollof dellortnoc raey-ruof a fo stluser :nicymanak...

Ngày tải lên: 07/08/2014, 18:21

... test the null hypothesis of marginal symmetry, namely that the probability of an observation being classified into cell [i,j] in the contingency table is the same as the probability of being classified ... infected in the routine samples, there was a good correlation between routine samples and 5-sock -samples A lower number of Salmonella positive socks in the routine samples than in the 5-sock -samples ... in contingency tables, cross-classified as Salmonella positive/negative Several methods are available for testing and comparing the agreement of the applied sample types In the current study the...

Ngày tải lên: 12/08/2014, 15:20

... 2009) 22 The idea is that where the location of the unmapped read may span the breakpoint of the CNV SR analysis has the advantage of being able to pinpoint the location of the breakpoints Assembly-based ... STATISTICAL METHODS FOR THE DETECTION AND ANALYSES OF STRUCTURAL VARIANTS IN THE HUMAN GENOME TEO SHU MEI A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS GRADUATE SCHOOL FOR INTEGRATIVE ... that the costs associated with down-handling, storing and analysis of the data could be more than the production of the data There is still a need for the development of new statistical/bioinformatics...

Ngày tải lên: 09/09/2015, 10:14

... relates to the index of the intensity of HPV infection This ratio of any specimen represents empirically a relative measure of the viral load in it Cut off value (RLU of specimen/ mean RLU of PC) ... expression of the viral E6 and E7 oncoproteins, which cause deregulation of the cell cycle by inactivating p53 and pRb respectively [9] Integration often disrupts the integrity and expression of the ... pg/ml of sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 is negative for HR HPV; Cutoff ratio greater than 1.0 positive for HR HPV The cut off ratio...

Ngày tải lên: 12/08/2014, 01:22

... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated ... recommend the Ames test using these sensitive strains (especially YG1041 and/or YG1042) in combination with the standard tester strains (TA98 and TA100 series), in order to detect the mutagenicity of ... ogttST::Cmr) strain (deficient in O6-methylguanine DNA methyltransferase) sensitive to alkylating chemicals These strains were kindly supplied by Dr T Nohmi of the National Institute of Health Sciences...

Ngày tải lên: 05/09/2013, 08:40

... patterns (the lack of their long-range order and their heterogeneity may explain the poor re¯ection hump obtained in XRD) Typically, as seen in the ®gure, a region containing ca Ê 6.93 A inter-planar ... when the temperature of the Ag catalyst is increased to 250 8C, the sensitivity of the WO3 sensor kept at 150 8C 71 increased by three times Without heating the Ag catalyst or bypassing the gas ... the concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the literature [18]...

Ngày tải lên: 19/03/2014, 16:47

... QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques were positive for the presence of an RV2 rhadinovirus In all of these six cases, both ... range of the assay the MfaRV2 and 72% (r2 = 0.929) for the PcyRV2, however, the low levels of virus in these samples made it difficult to accurately determine the efficiencies, as indicated by the ... using the sum of the errors of the viral and OSM copy number determinations, as described in Materials and Methods * These results, while positive for both duplicates, were outside of the linear...

Ngày tải lên: 18/06/2014, 22:20

... improving the efficiency of troop movement During the 1950s there was a need for an improved understanding of locomotion for the treatment of World War II veterans The classic work at the University of ... University of California [5,6] provided a tremendous resource of knowledge related to the mechanics of human movement The work at the University of California formed the basis for many of the fundamental ... skin The movement of the markers is typically used to infer the underlying relative movement between two adjacent segments (e.g knee joint) with the goal of precisely defining the movement of the...

Ngày tải lên: 19/06/2014, 10:20

... QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques were positive for the presence of an RV2 rhadinovirus In all of these six cases, both ... range of the assay the MfaRV2 and 72% (r2 = 0.929) for the PcyRV2, however, the low levels of virus in these samples made it difficult to accurately determine the efficiencies, as indicated by the ... using the sum of the errors of the viral and OSM copy number determinations, as described in Materials and Methods * These results, while positive for both duplicates, were outside of the linear...

Ngày tải lên: 20/06/2014, 04:20

... assistance in providing information regarding the cocoa industry in Vietnam and in the establishment of solar driers and fermenting boxes at their demonstration farm sites in Ben Tre Province HARVESTING ... darken in colour during drying as the majority of browning reactions will occur during this process DRYING OF COCOA Drying of cocoa is an important step in cocoa processing as some of the reactions ... on the percentage of fully brown beans in the cut test 6: The effect of spreading of beans for two hours, prior to placement in boxes, on fermentation temperatures 7: The effect of spreading of...

Ngày tải lên: 21/06/2014, 06:20

... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... proteinase K pre-treatment, and overnight hybridization The practicality of digoxigenin-labeled probe is better than those of biotin-labeled probe in the hybridization assay for the detection of ... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled...

Ngày tải lên: 07/08/2014, 17:22

... saw ecnabrosba ehT noitulos )SDS( etaflus lycedod muidos %1 a fo lµ 05 gnidda yb dehcneuq saw noitcaer cinegomorhc eht dna ,nim 51 rof detabucni erew setalp ehT dedda saw )ASU ,LPK ;tanoflusnilozihtneb-lyhte-3-id-oniza-'2.2( ... 1.0/05DICT 002 gniniatnoc noisnepsus suriv 9981VK a fo emulov lauqe na htiw dexim erew ares eht fo snoitulid dlof-owt laireS sllec 401FT gnisu setalporcim llew-69 a ni demrofrep saw nim 03 rof Co65 ta ... sisongaid -ores eht ni esu laitnetop sti rof detagitsevni saw ASILE-I VEJ eht rof depoleved saw dohtem lacigolores tneinevnoc dna elpmis a ,nosaer siht roF selpmas fo rebmun detimil a htiw noitazidradnats...

Ngày tải lên: 07/08/2014, 18:21

... sensitivity of the assay The results of our study indicated that the lateral flow biosensor assay was effective, even in the detection of 10 MAP organisms in the spiked fecal samples Apart from the spiked ... assay was run in triplicate with μl of synthetic DNA with different concentrations ranging from 1-1,000 fmol μ/l The limit of detection was determined using the signal obtained for the negative ... fecal samples containing 10 organisms of MAP RFR: reflectometer reading, CFU: colony forming units A biosensor assay for the detection of Mycobacterium avium subsp paratuberculosis in fecal samples...

Ngày tải lên: 07/08/2014, 23:22

... participated in the design of the registry study and are involved in continuing optimization NW is the study chairman of RiSK, developed the design of the registry study, is involved in continuing optimization ... have been integrated into most of the study protocols of the GPOH that are relevant in view of radiotherapy Therefore additional declarations of consent are not necessary in Germany The first ... centres of the GPOH study trials The documentation forms of these patients are asked for in the departments of radiotherapy if no documentation has been performed Parents' declarations of consent for...

Ngày tải lên: 09/08/2014, 09:22

... of dominance, before the experiment is designed, then maximum power will be attained by limiting the segregating subpopulation to the single backcross showing segregation However, the power of ... emphasized These curves are nearly linear for power under 70% and, in this linear part, the slope (ie the gain in power = = = per extra individual measured) increases with a The resulting increase in ... derives from the level of the within-group variance This variance for the F2 can be estimated in the parental and Fl groups in the homoskedastic model, but not in the heteroskedastic model In the latter,...

Ngày tải lên: 09/08/2014, 18:21

... SBEIIa-D(A201T) the amino acid change induced by the EMS treatment is located in the region of the N-terminal domain of the glycogen branching enzyme family, reported to be essential for the size of the ... product showed the inclusion of the intron V, whereas the smaller one was found to contain a deletion of the first seven nucleotides of exon VI The presence of the intron V in the longer transcript ... crystallographic structure of glycogen branching enzyme of E coli, the model protein for branching enzyme family [27] On the basis of these information it has been determined that the amino acid changes...

Ngày tải lên: 11/08/2014, 11:21

... suggesting less virus and lower sensitivity, and no differences in the timing of the collections or in the age or weight of the children In any case, the detection rates of the two collection methods ... losing the sample when respiratory episodes occur Secondly, the samples can be obtained immediately after the onset of the first signs and symptoms, thus favouring the identification of the infectious ... and the CT values were similar in the samples collected by the pediatrician and parents Furthermore, the direct involvement of parents significantly increased the patient's acceptance of the...

Ngày tải lên: 12/08/2014, 04:20

... differentiate the GPV vaccine strain from the high-virulence strain; nonetheless, we could perform the study on the dynamic distribution of GPV in vivo using these methods, because the animals ... permits the simultaneous detection and quantification of DNA It is useful for understanding the pathogenesis of the disease and the Previous studies have examined the distribution of GPV in infected ... resulted in the presence of clear and distinct amplified fragments (441 bp) on the agarose gel The detection limit of the FQ-PCR was determined based on the highest dilution that resulted in the presence...

Ngày tải lên: 12/08/2014, 04:20

... developed for the specific detection of PVM in various potato samples and for the confirmation of PCR amplicons The efficacy of RT-PCR for indexing seed potato samples on large scale for PVM was ... Discussion In this study, PVM was detected in several potato samples from an experimental farm in the province of Page of Figure Sensitivity of RT-PCR using primer set PVM3/PVM4 for detecting PVM in ... reliable detection of PVM RNA by RT-PCR The approach of using composite tuber samples will greatly reduce the cost and time associated with RT-PCR for indexing seed potato lots on a large scale for the...

Ngày tải lên: 12/08/2014, 04:21