methods for the analysis of mtdna

... with the technical aspects of data import and management, and refer the interested reader to the documentation of the software package prada for a thorough discussion of these [19] Selection of ... used for the different assays, each specifically measuring the phenotype of interest In the case of cell proliferation, the antibody detected the incorporation of the thymidine analog BrdU into the ... obtained either adaptively, for each well, or more globally, for the whole plate Because of the potential problems with over-fitting in the adaptive approach, we choose the latter, making use of the...

Ngày tải lên: 14/08/2014, 17:22

... β-CD-NH2 and the backward movement of the neutral sample partitioned in the micelles of sodium dodecyl sulfate (SDS) Stability of the inclusion complexes formed between the carrier and the solute ... displacer plug was introduced before sample injection to reduce the length of the concentrated sample zone and increase the peak height by slowing down the forward movement of the neutral sample associated ... With the high separation efficiency, high resolution and portability, CE has been proven a powerful method for the studies of many groups of compounds The improvement of sensitivity and the miniaturization...

Ngày tải lên: 14/09/2015, 10:36

... Where, Δp: the pressure difference; r: the inner diameter of the capillary; 20 t: the injection time; η: the viscosity of the buffer; L: the total length of the capillary In the case of all hydrodynamic ... macromolecules The transport of the solutes through the 15 capillary is based on the charge of the macromolecules, but the separation is dependent on the molecular size Besides the gel forming medium, other ... than the EOF as their velocity direction is the same as that of the EOF, so very rapid analysis times are therefore achieved with positively charged compounds The neutral compounds move at the...

Ngày tải lên: 14/09/2015, 10:37

... of 95 ml) The A280 to A260 ratio of the UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide ... compared to the other methods The SDS-extracted polymer included a small amount of material other than protein Furthermore, the steaming- and washing-extracted polymers contained large amounts of phosphorus ... buffer at a flow rate of ml/h and ml fractions were collected The void volume of the column was determined by the elution of Blue Dextran (Pharmacia) Other analyses - 147 - Journal of Water and Environment...

Ngày tải lên: 05/09/2013, 09:08

... assessment of the trajectory of interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) lineup of the accessions ... photosynthesis, gas exchange of plants was measured by infrared gas analysis Measurements were performed during the first h 508 of the light phase, representing the time period of photosynthetic ... terms of the absolute values but also in the progression of the acclimation process This is an important outcome, as it allows estimation of the impact of different metabolic responses on the improvement...

Ngày tải lên: 14/02/2014, 22:20

... used, rather than pure oxygen, avoiding the use of highly flammable or explosive gases in the laboratory The oxygen is added either in the spray chamber or using a T-connector before the torch ... suffer from the formation of new “cluster” species observed with the use of more reactive gases such as ammonia The performance of CRC based ICP-MS instruments cannot, however, match the detection ... analysis of the organic solvent is the preferred method of analysis, either untreated or simply diluted in a suitable solvent Direct organic solvent analysis demands some...

Ngày tải lên: 05/03/2014, 21:20

... scrutinized the German version of this text Prof Nirschl suggested the idea of this book Prof Reimert organized the continuation of the research unit after the DFG funding as well as Prof Kasper, Prof ... derivation due to the time-dependence of the unit vectors Using the product rule for the derivation and the fact that the time derivative of the unit vector is the cross product of !rf with this ... the scaling of the argument on index k of the measured data in reciprocal space As the sum is finite, it only represents an approximation of the delta function The position of the origin and the...

Ngày tải lên: 29/05/2014, 18:32

... program power system analysis These static load models are adequate for some types of power system dynamic analysis but not for others There remains a necessity for the development of accurate dynamic ... data 2.6 APPROACHES FOR STABILITY ANALYSIS The complete model for the stability analysis of a typical power system is a set of DAEs numbering on the order of thousands or tens of thousands At present, ... integration of the fault-on system In the second step, the direct methods directly determine, without numerical integration of the postfault system, whether the initial state of the postfault...

Ngày tải lên: 30/05/2014, 23:38

... knowledge related to the mechanics of human movement The work at the University of California formed the basis for many of the fundamental techniques currently used for the study of human locomotion ... patterns of locomotion requires new methods that would limit the risk of producing artifact due to markers or the constraints of the testing methods For example, the constraints of the laboratory ... Journal of NeuroEngineering and Rehabilitation 2006, 3:6 the capture and analysis of human movement For example, the Weber brothers (1836) reported one of the first quantitative studies of the temporal...

Ngày tải lên: 19/06/2014, 10:20

... on the percentage of fully brown beans in the cut test 6: The effect of spreading of beans for two hours, prior to placement in boxes, on fermentation temperatures 7: The effect of spreading of ... reveals the presence of certain defects which may cause off-flavours and indicates the degree of fermentation of the beans which has a bearing on the flavour and quality of the beans The International ... case of larger quantities of beans e.g 250kg, partitioned boxes (Illustration 13) are used In this case, turning of beans is performed by shovelling them from one side of the partition to the other...

Ngày tải lên: 21/06/2014, 06:20

... nonconductive, they severely distort the signal in the detector; therefore, many systems remove the bubbles before the optical light path However, if the system removes the bubbles at any point within the ... depends on the size and range of the scales With practice, the recommended nomographs can be read with a precision of 1% However, the overall bias of the results depends on the bias of the analytical ... data applied to the nomographs and the validity of the theoretical equations and the numerical constants on which the nomographs are based An approximate check of the bias of the calculations...

Ngày tải lên: 29/07/2014, 18:58

... Federation Standard Methods for the Examination of Water and Wastewater Organism Plate 10900 E Selected Taxonomic References The most useful references for the nonspecialist are listed below These references ... D.J REISH & J.W ANDERSON, eds 1994 Ecology of the Southern California Bight Univ California Press, Berkeley REISH, D.J 1995 Marine Life of Southern California, 2nd ed Kendall/ Hunt Publ Co., Dubuque, ... Water Environment Federation Standard Methods for the Examination of Water and Wastewater Algae General TAYLOR, W.R 1957 Marine Algae of the Northeastern Coast of North America, 2nd ed Univ Michigan...

Ngày tải lên: 29/07/2014, 18:58

... Methods for the Examination of Water and Wastewater Anacystis © Copyright 1999 by American Public Health Association, American Water Works Association, Water Environment Federation Standard Methods ... Methods for the Examination of Water and Wastewater Synedra © Copyright 1999 by American Public Health Association, American Water Works Association, Water Environment Federation Standard Methods for ... for the Examination of Water and Wastewater Ceratium © Copyright 1999 by American Public Health Association, American Water Works Association, Water Environment Federation Standard Methods for the...

Ngày tải lên: 29/07/2014, 18:58

... atomic weights of many elements are not invariant but depend on the origin and treatment of the material The standard values of Ar(E) and the uncertainties (in parentheses, following the © Copyright ... a volumes of the concentrated reagent are diluted with b volumes of distilled water to form the required solution Table B: PREPARATION OF UNIFORM SODIUM HYDROXIDE SOLUTIONS Normality of NaOH Solution ... water professionals worldwide Through educational and technical information programs, AWWA promotes the improvement of the quality and quantity of the drinking water supply Contact information:...

Ngày tải lên: 29/07/2014, 18:58

... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods for the Detection of Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... impact in many areas of parasitology, including the identification and classification of parasites, the diagnosis of infections, the epidemiology of parasites, the analysis of population genetic...

Ngày tải lên: 06/08/2014, 19:20

... volume of PBS The homogenized egg yolk was then mixed with two volumes of chloroform and incubated for hrs at room temperature After this incubation, the supernatant was collected To compare the ... respectively, after the first injection (Fig and 2) The titers of the vaccinated groups were three times higher than those of the control group Antibody profiles against OMP and DNT of B bronchiseptica ... Co., Germany) for 30 seconds After centrifugation of the sonicated bacterial culture broth at 8,000 rpm for 30 min, the supernatant was centrifuged at 20,000 rpm for hr at 40C and the pellet was...

Ngày tải lên: 07/08/2014, 15:20

... reflectance in the visible yet reduced reflectance in the NIR and SWIR regions of the spectrum Analysis of vegetation spectra 519 Figure Summary of F-ratio tests of significance of the variance of the ... differences in these factors are also conjectured for the eucalypt foliage These a priori expectations were tested with the null hypothesis of no treatment viz no species effect With the assumption of a ... colinearity, the approach should also reduce the dimensions of the data with a minimum of information loss It must accomplish this reduction without losing the ability to interpret the results Transforming...

Ngày tải lên: 08/08/2014, 01:22

... Morphological analysis In the second case of the figure, the heterozygote deficit (f) must be considered The frequency of the phenotype A+ is the sum of the frequency of the genotype P : +/+ All of the ... means of the comparison of the RAPD marker frequencies of a subsampling of these progenies and of the adult population Analysis of the morphological characters MATERIALS AND METHODS Sampling The ... classified in the space of the other species over the analyses Molecular analyses The allele frequencies of the RAPD loci in the adult trees of the stand, calculated according to the two hypotheses:...

Ngày tải lên: 08/08/2014, 18:21

... functioning of each method was not deteriorated by the other The relationship obtained with the comparison of the two methods over the whole period (n 589) is presented in figure The slope of the regression ... transpiration The combination of the two methods seems possible in the same experiment The heat balance for the small trunks, and transpiration calculation for small periods and Granier’s method for the ... Pairs of thermocouples inserted above and below the heater allow the measurement of the conduction flux (Qv) The radial outward flow (Qr) is calculated from thermopile meathermopile was composed of...

Ngày tải lên: 08/08/2014, 23:22

... validity of the new model for the analysis of the mechanisms of cartilage destruction by SFB Both MMP-1, capable of cleaving intact collagen [44], and MMP-3, responsible for the cleavage of other extracellular ... established the modified, present form of the model, performed the real-time PCR, the immunohistochemistry and the respective data analyses and wrote the manuscript D Pohlers performed some of the experiments ... higher amount of damage than the respective monocultures Collagen synthesis In view of an amplification of the potential net loss of collagen from the cartilage matrix, the synthesis of collagen...

Ngày tải lên: 09/08/2014, 01:22