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Tài liệu Báo cáo khoa học: "Logistic Online Learning Methods and Their Application to Incremental Dependency Parsing" doc
Ngày tải lên: 20/02/2014, 12:20
Cambridge.University.Press.An.Introduction.to.Law.and.Regulation.Text.and.Materials.Apr.2007.pdf
... field and an accessible and critical introduction to the subject. Morgan and Yeung set out a diverse and stimulating selection of materials and give them context with a compre- hensive and critical ... to Law and Regulation Text and Materials In recent years, regulation has emerged as one of the most distinct and important fields of study in the social sciences, both for policy-makers and for ... reading Baldwin, R. and Cave, M. 1999. Understanding Regulation: Theory, Strategy and Practice, Oxford: Oxford University Press. Baldwin, R., and McCrudden, C. 1987. Regulation and Public Law, London: Weidenfeld...
Ngày tải lên: 21/09/2012, 10:39
Glycoprotein Methods and Protocols - P1
... UV monitor and collect fractions using a fraction col- lector and subject to the appropriate carbohydrate and antibody analyses. 3.6. Analysis of Mucins Methods for the detection and analysis ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA). All these techniques have advantages and disadvan- tages. Solution methods ... serine proteases and esterases, including acetylcholinesterase, and should therefore be handled in a fume cupboard with extreme care! DFP is supplied in 1-g vials with a septum, and prior to dilution,...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P2
... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and Practice (45) provide comprehensive ... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucin staining. 1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... mammary gland, defined by antibodies to cytokeratins. J. Cell Sci. 75, 17–33. 45. Kiernan, J. A. (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK. 46. Andrew,...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P3
... volume, and superior wash- ing). For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should be included ... Chemical, Lectin, and Antibody Methods Michael A. McGuckin and David J. Thornton 1. Introduction Detection and quantitation of mucins can be important in both the research and clinical settings. ... 1 and 2 (2,3) of this volume for the preparation of secreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for a discussion of methods for mucin separation. 50 McGuckin and...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P4
... Adherent Mucous Gel Barrier 57 57 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc. , Totowa, NJ 5 The Gastrointestinal ... poly- L -lysine for 5 min and dried at room temperature. 3. Methods 3.1. Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections ( see Notes 1–4) This is a rapid and simple method ... will be greatly reduced and discontinuous. 7. Careful handling of the cryostat sections on the slide during fixing and staining is impor- tant because excessive washing and so on can remove mucus...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P5
... samples no. 1 and 2 (values a and e): x = g/c × a/e (no dimension) Biosynthesis and Secretion of Mucin 65 65 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: ... Humana Press Inc. , Totowa, NJ 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling Jan Dekker, B. Jan-Willem Van Klinken, Hans A. Büller, and Alexandra W. C. Einerhand 1. ... follows: a. Incubate in Blotto for 30 min, and incubate with antibody directed against nongly- cosylated peptide epitopes of the mucin of interest for 90 min. b. Wash two times for 15 min each. c. Incubate...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P6
... small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer. 2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... with these 77 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc. , Totowa, NJ Separation and Identification ... other identified mucins. Separation and Identification of Mucin 77 7 Separation and Identification of Mucins and Their Glycoforms David J. Thornton, Nagma Khan, and John K. Sheehan 1. Introduction This...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P7
... sizes and should be chosen according to sample volume and concentration. Heterogeneity and Size Distribution of Gel-Forming Mucins 91 mucins on these grids; and the addition of contrast, including ... 2.400 a The values for ρ and η rel. are from ref. 14. Heterogeneity and Size Distribution of Gel-Forming Mucins 87 87 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: ... sandwhich technique for preparing macromolecules for rotary shadowing. J. Ultrastruct. Res. 91, 66–76. 14. Kawahara K and Tanford C. (1966) Viscosity and density of aqueous solutions of urea and...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P8
... 113 113 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc. , Totowa, NJ 10 Amino Acid Analysis of Mucins Jun X. Yan and ... 2 min for pump and column equilibration with the initial composition of the mobile phase. 5. Figures 1 and 2 show typical chromatograms of the separation of amino acid standards and hydrolyzed ... samples and Dr. Andrew Gooley and Prof. Keith Williams for their support. References 1. Yan, J. X., Wilkins, M .R., Ou, K., Gooley, A. A., Williams, K. L., Sanchez, J C., Golaz, O., Pasquali, C., and...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P9
... L., and Gooley, A. A. (1996) Sequencing Glycopeptides 121 121 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc. , ... Kirk, M., Barnes, S., Lowary, T. L., and Hart, G. W. (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry. ... arylamine and should be avoided. 15. The EDC reagent should be mixed with the reaction buffer just prior to coupling. 16. Incubation at 4°C increases the initial yield (7). 17. To incorporate...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P10
... 129 129 From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited by: A. Corfield © Humana Press Inc. , Totowa, NJ 12 Synthetic Peptides for the Analysis and Preparation of ... monoclonal antibodies (mAbs) and fragments have been produced by various methods. Some of these have found applications for radio- immunoscintigraphy and targeted therapy of cancer, and others have been ... for the Analysis and Preparation of Antimucin Antibodies Andrea Murray, Deirdre A. O’Sullivan, and Michael R. Price 1. Introduction Since the mid-1980s, the family of high molecular weight glycoproteins...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P11
... tip, and incubate the cells on ice for 30 min. 5. Heat shock the reactions in a 37˚C water bath for 20 s, and then incubate them on ice for 2 min. 6. Add 950 µL of LB to each reaction tube and incubate ... Woitach, J. T., Davidson, E. A., and Bhavanandan, V. P. (1990) Cloning and cDNA sequence of a bovine submaxillary gland mucin-like protein containing two distinct domains. Proc. Natl. Acad. Sci. ... polymerase, and incubate the reaction at 72˚C to allow extension of the linked templates. Add 2 µg each of primers 5 and 6, and continue the PCR with 30 cycles at 94˚C for 30 s, 50˚C for 1 min and 72˚C...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P12
... Nakamura, M., and Ohkura, Y. (1987) Fluoro- metric high performance liquid chromatography of N-acetyl- and N-glycolyl neuraminic acids and its application to their microdetermination in human and animal ... acid (Neu5Gc), and keto-deoxy- nonulosonic acid (Kdn), respectively. Substituents of the hydroxyl groups present on From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: ... at 2°C/min. 5. Use injector and detector temperatures of 240 and 250°C, respectively. 174 Michalski and Capon Table 4 Elution Gradient for the Separation of Unreduced and Reduced Monosaccharides Time...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P13
... M. J. and Hounsell, E. F. (1998) HPLC and HPAEC of oligosaccharides and glyco- peptides, in Glycoscience Protocols (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 79–100. 14. Kakehi, K., and Honda, ... 0.5 cm column) and elute with three column vol of HPLC-grade water. 5. Collect the effluent and eluent and then pool (2.5-mL volume). 6. Load onto a PD10-Sephadex GM25 column and elute with HPLC-grade ... cm) in a water jacket at 55°C and HPLC pump with refractive index and fluorescence detectors. 5. GlycoSepH™ and GlycoSepC™ HPLC column (Oxford GlycoSciences). 3. Methods 3.1. Alkaline Borohydride...
Ngày tải lên: 08/10/2012, 10:25
Glycoprotein Methods and Protocols - P14
... Continued. Analysis of Mucin-Type O -Linked Oligosaccharides 193 atoms; Gal H-3 and H-4 atoms; Fuc H-5 and H-6 atoms; NeuAc H-3ax and H-3eq atoms; and CH 3 of the acetamido groups. The first step of spectrum ... or three at the most) and the facilities that are available in the laboratory environment will define From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins Edited ... was introduced in the 1970s and rapidly received a large application for analyzing the sequence of N-acetyllactosamine- and oligomannosidic-type glycans. Originally, and before the development...
Ngày tải lên: 08/10/2012, 10:25
