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Tài liệu Báo cáo khoa học: "Logistic Online Learning Methods and Their Application to Incremental Dependency Parsing" doc

Cambridge.University.Press.An.Introduction.to.Law.and.Regulation.Text.and.Materials.Apr.2007.pdf

... field and an accessible and critical introduction to the subject. Morgan and Yeung set out a diverse and stimulating selection of materials and give them context with a compre-hensive and critical ... to Law and RegulationText and Materials In recent years, regulation has emerged as one of the most distinct and importantfields of study in the social sciences, both for policy-makers and for ... readingBaldwin, R. and Cave, M. 1999. Understanding Regulation: Theory, Strategy and Practice, Oxford: Oxford University Press.Baldwin, R., and McCrudden, C. 1987. Regulation and Public Law, London:Weidenfeld...

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Glycoprotein Methods and Protocols - P1

Danh mục: Môi trường

... UV monitor and collect fractions using a fraction col-lector and subject to the appropriate carbohydrate and antibody analyses.3.6. Analysis of Mucins Methods for the detection and analysis ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linkedimmunosorbent assays (ELISA). All these techniques have advantages and disadvan-tages. Solution methods ... serine proteases and esterases, includingacetylcholinesterase, and should therefore be handled in a fume cupboard with extremecare! DFP is supplied in 1-g vials with a septum, and prior to dilution,...

Glycoprotein Methods and Protocols - P2

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... such as Culling’s Handbook ofHistopathological and Histochemical Techniques (including museum techniques) (36) orKiernan’s Histological and Histochemical Methods: Theory and Practice (45) providecomprehensive ... nature and limitations of the individual techniques, and (2) interpretation and reporting of mucinstaining.1.1. Nature and Limitations of Mucin-Staining Methods Mucin histochemistry, lectin, and ... mammary gland, defined by antibodies tocytokeratins. J. Cell Sci. 75, 17–33.45. Kiernan, J. A. (1990) Histological and Histochemical Methods: Theory and Practice,Pergamon, Oxford, UK.46. Andrew,...

Glycoprotein Methods and Protocols - P3

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... volume, and superior wash-ing). For quantitation and comparison across blots, a standard in the same buffer assamples should be titrated for use as a standard curve, and samples should be included ... Chemical, Lectin, and Antibody Methods Michael A. McGuckin and David J. Thornton1. IntroductionDetection and quantitation of mucins can be important in both the research and clinical settings. ... 1 and 2 (2,3) of this volume for the preparation ofsecreted and membrane-associated mucins, respectively, and to Chapter 7 (4) for adiscussion of methods for mucin separation.50 McGuckin and...

Glycoprotein Methods and Protocols - P4

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... Adherent Mucous Gel Barrier 5757From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc. , Totowa, NJ5The Gastrointestinal ... poly-L-lysine for 5 min and dried at room temperature.3. Methods 3.1. Observation and Measurementof Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4)This is a rapid and simple method ... will be greatlyreduced and discontinuous.7. Careful handling of the cryostat sections on the slide during fixing and staining is impor-tant because excessive washing and so on can remove mucus...

Glycoprotein Methods and Protocols - P5

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... samples no. 1 and 2 (values a and e):x = g/c × a/e (no dimension)Biosynthesis and Secretion of Mucin 6565From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: ... Humana Press Inc. , Totowa, NJ6Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic LabelingJan Dekker, B. Jan-Willem Van Klinken,Hans A. Büller, and Alexandra W. C. Einerhand1. ... follows:a. Incubate in Blotto for 30 min, and incubate with antibody directed against nongly-cosylated peptide epitopes of the mucin of interest for 90 min.b. Wash two times for 15 min each.c. Incubate...

Glycoprotein Methods and Protocols - P6

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... small gel, use 1.6 g ofagarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL ofbuffer.2. Leave to cool before pouring (hand hot) and insert well-forming comb (see ... with these77From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc. , Totowa, NJSeparation and Identification ... other identified mucins.Separation and Identification of Mucin 777Separation and Identificationof Mucins and Their GlycoformsDavid J. Thornton, Nagma Khan, and John K. Sheehan1. IntroductionThis...

Glycoprotein Methods and Protocols - P7

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... sizes and should be chosen according tosample volume and concentration.Heterogeneity and Size Distribution of Gel-Forming Mucins 91mucins on these grids; and the addition of contrast, including ... 2.400aThe values for ρ and ηrel. are from ref. 14.Heterogeneity and Size Distribution of Gel-Forming Mucins 8787From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: ... sandwhichtechnique for preparing macromolecules for rotary shadowing. J. Ultrastruct. Res. 91,66–76.14. Kawahara K and Tanford C. (1966) Viscosity and density of aqueous solutions of ureaand...

Glycoprotein Methods and Protocols - P8

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... 113113From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc. , Totowa, NJ10Amino Acid Analysis of MucinsJun X. Yan and ... 2 min for pump and column equilibration with the initial composition ofthe mobile phase.5. Figures 1 and 2 show typical chromatograms of the separation of amino acid standards and hydrolyzed ... samples and Dr. Andrew Gooley and Prof. Keith Williams for their support.References1. Yan, J. X., Wilkins, M .R., Ou, K., Gooley, A. A., Williams, K. L., Sanchez, J C.,Golaz, O., Pasquali, C., and...

Glycoprotein Methods and Protocols - P9

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... L., and Gooley, A. A. (1996)Sequencing Glycopeptides 121121From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc. , ... Kirk, M., Barnes, S., Lowary, T. L., and Hart, G.W. (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides byβ-elimination and tandem electrospray mass spectrometry. ... arylamine and should be avoided.15. The EDC reagent should be mixed with the reaction buffer just prior to coupling.16. Incubation at 4°C increases the initial yield (7).17. To incorporate...

Glycoprotein Methods and Protocols - P10

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... 129129From: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited by: A. Corfield © Humana Press Inc. , Totowa, NJ12Synthetic Peptides for the Analysis and Preparationof ... monoclonal antibodies (mAbs) and fragments have beenproduced by various methods. Some of these have found applications for radio-immunoscintigraphy and targeted therapy of cancer, and others have been ... for the Analysis and Preparationof Antimucin AntibodiesAndrea Murray, Deirdre A. O’Sullivan, and Michael R. Price1. IntroductionSince the mid-1980s, the family of high molecular weight glycoproteins...

Glycoprotein Methods and Protocols - P11

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... tip, and incubate the cells on ice for 30 min.5. Heat shock the reactions in a 37˚C water bath for 20 s, and then incubate them on ice for 2 min.6. Add 950 µL of LB to each reaction tube and incubate ... Woitach, J. T., Davidson, E. A., and Bhavanandan, V. P. (1990) Cloning and cDNA sequence of a bovine submaxillary gland mucin-like protein containing twodistinct domains. Proc. Natl. Acad. Sci. ... polymerase, and incubate the reaction at 72˚C to allow extension of the linkedtemplates. Add 2 µg each of primers 5 and 6, and continue the PCR with 30 cycles at 94˚Cfor 30 s, 50˚C for 1 min and 72˚C...

Glycoprotein Methods and Protocols - P12

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... Nakamura, M., and Ohkura, Y. (1987) Fluoro-metric high performance liquid chromatography of N-acetyl- and N-glycolyl neuraminicacids and its application to their microdetermination in human and animal ... acid (Neu5Gc), and keto-deoxy-nonulosonic acid (Kdn), respectively. Substituents of the hydroxyl groups present onFrom: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: ... at 2°C/min.5. Use injector and detector temperatures of 240 and 250°C, respectively.174 Michalski and CaponTable 4Elution Gradient for the Separationof Unreduced and Reduced MonosaccharidesTime...

Glycoprotein Methods and Protocols - P13

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... M. J. and Hounsell, E. F. (1998) HPLC and HPAEC of oligosaccharides and glyco-peptides, in Glycoscience Protocols (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 79–100.14. Kakehi, K., and Honda, ... 0.5 cm column) and elute with threecolumn vol of HPLC-grade water.5. Collect the effluent and eluent and then pool (2.5-mL volume).6. Load onto a PD10-Sephadex GM25 column and elute with HPLC-grade ... cm)in a water jacket at 55°C and HPLC pump with refractive index and fluorescence detectors.5. GlycoSepH™ and GlycoSepC™ HPLC column (Oxford GlycoSciences).3. Methods 3.1. Alkaline Borohydride...

Glycoprotein Methods and Protocols - P14

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... Continued.Analysis of Mucin-Type O-Linked Oligosaccharides 193atoms; Gal H-3 and H-4 atoms; Fuc H-5 and H-6 atoms; NeuAc H-3ax and H-3eqatoms; and CH3 of the acetamido groups.The first step of spectrum ... or three atthe most) and the facilities that are available in the laboratory environment will defineFrom: Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The MucinsEdited ... was introduced in the 1970s and rapidlyreceived a large application for analyzing the sequence of N-acetyllactosamine- and oligomannosidic-type glycans.Originally, and before the development...