metal organic interface formation studied in situ by resonant raman spectroscopy

... to quantitate protein-protein interactions in vivo The thesis contains six chapters and is structured into the following sections: Chapter introduces the basic principles and instrumentation of ... molecules in the system Chapter applies SW-FCCS to quantitate protein-protein interactions It is divided into two parts In the first part, the binding between a small GTPases protein vii Cdc42, ... photobleaching and endogenous proteins All these factors influence the quality of the determined Kd Major findings include quantitating the fraction of non-fluorescent red fluorescent proteins (mRFP...

Ngày tải lên: 10/09/2015, 08:38

... heal up In order to gain a deep insight into the origin of the cracks, stress distribution along depth was measured by cross-sectional micro -Raman spectral Raman spectra were conducted in μm steps ... stress and exceeding the critical thickness during the growth process We should explain the origin of the tensile stress Many authors observed intrinsic tensile stress using in situ measurements ... The origin of cracks in GaN film grown by MOCVDHVPE system has been analyzed by SEM, HR-XRD, Raman, and CL The stress distribution was obtained by cross-sectional Raman spectra According to the...

Ngày tải lên: 21/06/2014, 06:20

... THANH HAI, DOAN MINH THUY, WANDELT KLAUS Experimentally, scanning tunneling microscopy (STM) invented in the early 1980s by Binnig and Rohrer [16, 17, 18] is a powerful tool for the investigation ... electrochemical scanning tunneling microscopy (EC-STM) described in detail by Wilms et al [20] In order to eliminate the in uence of oxygen as well as acoustic and electromagnetic interferences from ... electrochemical STMs (EC-STM) in order to gain better research results Exemplarily, a home-built EC-STM system introduced by Wilms et al in 1999 [20] has been applied since then by the Wandelt group,...

Ngày tải lên: 30/10/2015, 20:57

... into the reactor chamber, an ex -situ cleaning procedure was performed using solvents (trichloroethylene, acetone, and methanol) for degreasing followed by a piranha etch to remove any remaining ... conduction and bring out the SS-based TI effects may include various doping engineering4,34, as well as thinning down the MOCVD films (e.g., to tens of nm) The transport measurements reported in this ... best fit (as shown by the black dash line in Fig 3) to the experimental data The temperature dependence is dominated by the exponential term (phonon scattering) and the fitting parameter 𝜃 corresponds...

Ngày tải lên: 05/03/2014, 21:20

... induced by the deposition process The o-rocking curves are shown in the inset of Fig It shows that ZnO grows in single c-axis orientation with the c-axis normal to the sapphire basal plane, indicating ... RENISHAW-Ramascope at room temperature in back-scattering configuration by using 514.5 nm Ar+ laser line excitation with an arriving power of about 70 mW The scattered light was detected by a water-cooled charge ... slight increase in intrinsic defects in ZnO due to nitrogen doping PL spectra under different excitation powers were performed We not find any shift of the position of the UV emission peak The integrated...

Ngày tải lên: 05/03/2014, 21:20

... X Ding et al / Journal of Colloid and Interface Science 320 (2008) 341–345 by oxidatively polymerizing aniline using ammonium peroxodisulfate as oxidant (NH4 )2 S2 O8 [23] There, the obtained ... particles is shown in Fig By taking advantage of the micelle reaction system, we firstly obtained colloidal PANI doped with DBSA by the one-step chemical oxidative polymerization of aniline in the presence ... of the resulting PANI/magnetite nanorods prepared by in situ self-assembly method As shown in Fig 2A, PANI/magnetite nanorods has an unique nanorods structure in shape, ca 80 nm in diameter and...

Ngày tải lên: 20/03/2014, 13:04

... The magainin %CR values were between two and four times higher than those induced by melittin or KAL, an indication that magainin is located predominantly at the lipid–water interface, causing enhanced ... data Magainin, for example, induced the fastest quenching among the three peptides in both vesicle models (Fig 6A), probably reflecting its pronounced lipid–water interface binding and interactions ... within the vesicles promotes deeper insertion of melittin and KAL, but does not seem to affect the strong interfacial binding of magainin For example, at peptide concentrations of lM, melittin induced...

Ngày tải lên: 30/03/2014, 20:20

... now being exploited in cancer, attempting to modulate their expression, reintroducing microRNAs lost in cancer, or inhibiting oncogenic microRNAs by using anti-micro oligonucleotides [38] In a ... for in situ hybridization analysis In Situ Hybridization (ISH) In situ hybridization was performed following the protocol developed by Nuovo et al [25], with some minor adjustments Digoxigenin ... semiquantitatively for cytoplasmic staining on computer screen The dominant staining intensity in tumor cells was scored as: = negative; = weak; = intermediate; = strong (Figure 1) In case of disagreement...

Ngày tải lên: 18/06/2014, 16:20

... osteoinduction by radiographic examination Microcomputed Tomography inspection of explanted spines exposed to BMP-2 was performed taking an average hours/spine (including preparation, scanning, ... the posterior paraspinal muscles resulted in situ endochondral ossification adjacent to vertebrae Mineralized tissue of varying degrees was present by radiographic examination in all treatment animals ... spines was performed first to determine optimal spinal flexion/extension conditions for testing fusion integrity Maximal intervertebral motion of untreated spines was observed at 150°of spinal...

Ngày tải lên: 20/06/2014, 04:20

... osteoinduction by radiographic examination Microcomputed Tomography inspection of explanted spines exposed to BMP-2 was performed taking an average hours/spine (including preparation, scanning, ... the posterior paraspinal muscles resulted in situ endochondral ossification adjacent to vertebrae Mineralized tissue of varying degrees was present by radiographic examination in all treatment animals ... spines was performed first to determine optimal spinal flexion/extension conditions for testing fusion integrity Maximal intervertebral motion of untreated spines was observed at 150°of spinal...

Ngày tải lên: 20/06/2014, 07:20

... results imply that most of the oxygen-containing groups of GO were reduced by the photoinduced electrons RGO obtained by in- situ reduction was analyzed by HRTEM Figure 3b shows a low magnification ... the GO film, the photoinduced electrons could be captured by GO and result in the reduction of GO Inspired by this, in this contribution, we provide an easy approach to an in- situ photoelectrochemical ... of the oxygen-containing groups are removed In addition, the atomic ratio of carbon and oxygen (C/O), obtained by taking the ratio of C1s and O1s peak areas in XPS spectra, increases from 2.7...

Ngày tải lên: 21/06/2014, 17:20

... through the formation of an ultra-thin oxidation layer caused by including acetone in the quenching solution EDS data for the resulting NPs indicate the elemental oxygen appearing in those NPs ... mixture P2 H1 H2 Inlet V2 Reducing agent and stability Inlet TC1 N2 In N2 In Y-mixer N2 Out Reaction channel N2 Out TC2 N2 Out P3 N2 in V3 Quenching inlet Cold quenching Y-mixer Quenching channel ... ROCS and SEM by the Chinese Education Ministry, and Innovative Research Team of Chinese Education Ministry in University (IRT0512) at Beihang University Y Song also appreciates the kind suggestions...

Ngày tải lên: 21/06/2014, 20:20

... Conclusion In conclusion, we have demonstrated a novel method to form p–n junction NW diodes by combining two well- 123 established doping techniques in- situ doping and ion implantation, in succession ... should result in B-doped—intrinsic (p–i) type NWs, since the boron diffusion in silicon is negligible at 525°C [7], i.e., the B atoms incorporated in the lower half will not diffuse into the upper ... from being implanted with P ions from the side This step effectively eliminates the possibility of lateral doping that is almost unavoidable in purely in- situ doping of NWs successively by two...

Ngày tải lên: 22/06/2014, 00:20

... dominance of the parent hyphae, regular, but not too frequent branching in correlation to septation, and negative autotropism between neighboring hyphae Three different models of hyphal growth in ... solutes from the fungus The solute efflux may be active or by leakage Cytokinins secreted by the fungus L.- .- I’L 1’ I tree root may influence ion release (Pohleven, 1988) Transport to the cortical ... activity in the Pinus sylvestrisltaccaria laccata ectomycorrhizal association New P O8, 329-334 7yfo/ / an although The investigations have been supported by grants from the Deutsche Forschungsgemeinschaft...

Ngày tải lên: 09/08/2014, 04:20

... determine whether cav-1 and cav-2 are in close apposition in airway epithelial cells in situ, thereby indicating an association of both proteins and formation of hetero-oligomers Distinct increase ... agonist binding [21,22] These receptors are involved in the regulation of ciliary function [23,24] In addition, several proteins involved in Ca2+-dependent signaling processes, including the Ca2+-pump ... associated in ciliated and in basal cells, indicating that both proteins are involved in the formation of caveolae Interestingly, we found considerably higher expression of cav2 mRNA than cav-1 mRNA, indicating...

Ngày tải lên: 12/08/2014, 16:20

... and approved the final article for publication Competing interests There are no financial or non-financial competing interests for any of the authors The study was financed by the Karola Jørgensen’s ... also in other areas of medical and surgical therapies Furthermore, in situ simulation is suitable for identifying logistical and operational problems in institutions as the ongoing merging of ... for in situ simulation [31] Finally, application of in situ simulation can provide considerable information with identification of systemlevel problems and performance assessment not only in cardiac...

Ngày tải lên: 13/08/2014, 23:20

... & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon Res 6, 349-360 Lin C.C., Draper P.N & De Braekeleer M (1985) ... method thereby, permitting more reproducible results The high specific activity could partly explain the efficiency of in situ hybridization This observation confirms the one given by Lin et al ... for in situ, hybridization has been described elswhere (Gellin et al., 1985), and we added only some modifications concerning the washings after hybridization: the slides were first rinsed in x...

Ngày tải lên: 14/08/2014, 19:23

... family of signaling proteins Upon agonist binding to β receptors, a signaling cascade is launched involving the heterotrimeric G protein Gq, which activates phospholipase C, increasing inositol trisphosphate ... (DβH) as shown in Figure DβH is the enzyme of the catecholamine biosynthesis pathway that converts dopamine into norepinephrine in adrenergic and noradrenergic cells Immune staining against these ... of injury[10] Second, instillation of a tetrodotoxin-containing solution into the pericardial sac acutely interrupts sympathetic responses of the heart[11] Third, myocardial infarctions involving...

Ngày tải lên: 24/08/2014, 12:57