mesh optimisation using genetic algorithms

... Secondly, the relative technical and scale efficiencies of a sample of Chinese banks are analyzed using a non-parametric technique, called Data Envelopment Analysis (DEA) This technique allows the ... the rural and urban credit cooperatives societies, other commercial banks, regional banks and housing The Agricultural Bank of China’s operations were previously subsumed within the Rural Bank ... “rice-bowl” environment, depending on the governments for their welfare needs, which included housing, medical and children’s education This eventually led to adverse selections, that is, biased...

Ngày tải lên: 11/09/2015, 14:38

... Statistical analyses Statistical analyses were performed using a one-way ANOVA parametric test and Bonferroni/Newman-Keuls analysis of variance using the GraphPad Prism software, version 2.0, as well ... all of the animals, using autologous fibroblasts as targets Many vaccination trials have been performed on patients with cervical cancer, genital warts or papillomas [51,52], using the HPV-16 E6/E7 ... animals by a single intradermal injection The presence of E6 and E7 genes in VX2T cells was assessed using CRPV primers V234 (5'-CTG-AGA-TCGCAA-CGC-ATT-GC-3') and V235 (5'-GCC-TGG-ATATAA-TCC-AAG-TT-3')...

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 07/08/2014, 21:21

... MAPK8, SMAD2, IL2RG, PLCB1, and ATF5) using real-time reverse transcription-polymerase chain reaction (RT-PCR) PCRs were performed as previously described using a Mastercycler® ep realplex (Eppendorf, ... of the supplier (Affymetrix, Santa Clara, CA, USA) Analysis of gene expression was carried out using U133A/B oligonucleotide arrays Hybridization and washing procedures were performed according ... Hewlett-Packard Company, Palo Alto, CA, USA) Background-corrected signal intensities were determined using the MAS 5.0 software (Affymetrix) Subsequently, signal intensities were normalized among arrays...

Ngày tải lên: 09/08/2014, 10:23

... Research Institute since 1984 (Tai et al, 1994) Not much is known about the genetic parameters and especially the genetic correlations for these traits in ducks Tai et al (1989) estimated heritabilities ... for of these traits in the first generation Lee et al (1992) estimated genetic parameters in each of the first generations, using variance component estimation method applied to a hierarchical ... applied to an animal model for predicting the genetic merit of candidates for selection in most species of farm animals For this purpose estimates of the genetic parameters in the base population...

Ngày tải lên: 09/08/2014, 18:22

... (A) and h (C) Cytosolic and nuclear extracts were immunoblotted using anti-p65 of NFB and anti-actin Extracts were immunoblotted using RCC1 indicating nuclear purity of the fractions (not shown) ... for the quantitative measurement of this cytokine in supernatants ChIP The ChIP assay was done using the Upstate-kit (Millipore, USA) according to manufacturer’s instructions Briefly, × 106/ml ... control Ab (Santa-Cruz Biotechnology) overnight at 4°C Immunoprecipitated complexes were collected using protein A-agarose beads The precipitates were extensively washed and then incubated in the...

Ngày tải lên: 11/08/2014, 08:22

... determined by RT-PCR using previously described methods [13] DNA was extracted from whole blood using a TIANamp bacteria DNA kit (TIANGEN) and RNA was extracted from the sera using a QIAamp viral ... increased host mortality Moreover, SS7 infection improved the distribution of the virus, thereby causing damage to more organs Therefore, secondary infection with SS7 enhances the pathogenicity of ... observation The pigs were monitored daily and scored for the severity of clinical respiratory disease by using a scoring system ranging from (normal) to (severe dyspnea and abdominal breathing) [22] In...

Ngày tải lên: 12/08/2014, 04:20

... excluded, as the ESOPO study was conducted by using QUS (quantitative ultrasound) measurements, given the lack of national epidemiologic studies performed by using DEXA (dual-energy x-ray absorptiometry), ... examined years were obtained from the Italian Institute for Statistics (ISTAT) Data were processed by using Stata (StataCorp, College Station, TX, USA) and Excel (Microsoft, Redmond, WA, USA) software ... systematically result in hospitalization, thus allowing researchers to perform epidemiologic analyses by using hospital discharge records [13] Conversely, only a small proportion of patients with osteoporotic...

Ngày tải lên: 12/08/2014, 15:22

... indicated The amount of protein was normalized using anti-actin antibody Figures below NCoA3 immunoblot indicated the results of the quantification using Image Tool (Syngene) software of the ratio ... quality was assessed using the Agilent Bioanalyzer 2100 and spectrophotometric analysis prior to cDNA synthesis Microarray experiments Microarray experiments were performed using the U133A microarrays ... http://www.retrovirology.com/content/2/1/73 immunoglobulins) Hybridizations were revealed using an ECL enhanced chemiluminescence kit (ECL, Amersham) The quantification was done using the Image Tools (Syngene) software Additional...

Ngày tải lên: 13/08/2014, 09:21

... for niche differentiation in microbial species [34] by increasing the genetic variability via the acquisition of foreign genetic material Finally, large CAGs (that is, containing 40 or more ORFs) ... were evaluated using in silico HGT simulations in order to test their performances under different genomic backgrounds The 119 genomes were analyzed and 100 simulations were performed using the core ... bacterial genomes (Figure 2b; Materials and methods) We performed two types of HGT simulations using all 11 different core genes datasets In the first simulation we chose 100 ORFs from the other...

Ngày tải lên: 14/08/2014, 21:20

... constructs and sub-constructs in this study have good theoretical support Nonetheless, future research using longitudinal data is recommended, as this would improve the understanding of the framework ... distribution would eliminate the issues of economic, environmental, and social costs (Azapagic 2003) Focusing on the Thai context of sustainability issues, Thailand ranked 23rd in the world for CO2 emissions, ... the management of distribution and transportation processes implicit in order processing, warehousing, packaging, and delivery, as well as product returns in order to minimize social and environmental...

Ngày tải lên: 28/11/2015, 14:01

...  3.28 days, respectively This suggested that using PSI as coagulant resulted to higher efficiency in mitigating 209 membrane fouling risk than using sole Fe(III) The decrease of fouling frequency ... kinetic batch experiments The pH of all solutions of the batch experiments were controlled to 7.0 using HCl and NaOH solutions during the preparation process 204 Long-term MBR experiments Fig shows ...  45     45/49.5  Analytical methods Protein and carbohydrate concentrations were measured using Lowry’s method and the anthrone-sulfuric acid reaction, respectively Iron and silicate were...

Ngày tải lên: 05/09/2013, 10:15

... then it makes sense to rush to the doctor and ask for a remedy Most of us are very great at focusing on the more important things in our lives, our career goals, our finances, our relationships, ... www.gettheedge.com How to Apply This Knowledge Quickly So far you’ve heard me rant on and on about using this stuff, well I’m going to keep on doing it! Not because I’m a maniac, but because I honestly...

Ngày tải lên: 07/11/2013, 21:15

... job), self-monitoring of capillary glycemia, foot self-care, medication adherence (was measured using Haynes-Sackett test [11]: ‘Most patients have difficulty taking tablets Do you have any difficulty ... cholesterol, HbA1c, SBP, and DBP The effect of the PRECEDE HPE was determined for these variables using the formula: mean value of the change in HPE PRECEDE - mean value of the change in conventional ... accepted level of significance was 0.05 or less, with 95% CI All the analyses were carried out using the intention-to-treat principle Statistical analysis of the data was carried out with SPSS...

Ngày tải lên: 05/03/2014, 22:21

... the difficulty of dealing with pollution created in one (autonomous) district or province but causing effects in another, and the environmental consequences of a “race to the bottom” for investment ... attributed to the provincial governor the power to use administrative coercion against someone causing environmental distress (Art 25) This gave rise to—probably unintended—overlapping competences ... These discharge their wastewater into the Citarum River, which flows from Sumedang to Bandung, causing loss of harvest to the farmers in the latter district After protests of farmers to the subdistrict...

Ngày tải lên: 29/03/2014, 21:20

... Thorens, Lausanne, Switzerland, using the DIG RNA labelling kit (Boehringer-Mannheim, Germany) Ó FEBS 2002 CDNA synthesis, PCR ampli®cations, and Southern-blot analysis Using random primers, the ®rst-strand ... until the insulin assays Insulin was measured by the radioimmunoassay method of Herbert et al [15], using rat insulin as a standard RNA isolation and Northern-blot analysis Total cellular RNA from ... adult male rats (200±225 g) by the procedure of Lacy & Kostianovsky [12] as modi®ed by Gotoh [13], using collagenase P (1±1.6 mgámL)1) and DNase I (1 mgámL)1) Pancreatic islets from fetuses and 5,...

Ngày tải lên: 31/03/2014, 15:20

... patients immunized with genetic vaccines encoding glycoprotein 100 (gp100) or MART-1, two melanoma-associated antigens We report our study on validation of the three methods using TIL cells alone ... protocol (Promega) using AMV Reverse Transcriptase with 25 μM of RT primer for IFNγ or CD8 Samples were stored at -15°C until further analysis Real Time RT-PCR analysis was performed using forward ... Time PCR analysis was done using ABI Prism 7700 software for mRNA quantification Additional statistical analysis was performed to examine assay accuracy and precision using Microsoft Excel Accuracy...

Ngày tải lên: 18/06/2014, 15:20

... liver failure [21,22] and ALS [23] Chronic stimulation of stem cell mobilization is not possible using agents such as G-CSF, due to cost and possible adverse effects such as thrombosis which would ... PBS with 0.5% BSA and resuspended in 100 ul of buffer for analysis Flow cytometry was performed using a Cell Lab Quant SC system (Beckman Coulter) equipped with 22 mW argon laser tuned at 488 ... (CD45-positive cells) Quantification of EPC based on colony forming ability EPC cultures were performed using a modification of the previously described method [28-31] Briefly, PBMC were plated on 24-well...

Ngày tải lên: 18/06/2014, 16:20

... Fourier transform infrared (FTIR) spectra were recorded using Bruker-EQUINOX55 spectrophometer PL spectra were recorded at room temperature using a 355-nm exciton wavelength of a Nd: YVO laser The ... (EFTEM) and high-resolution transmission electron microscopy (HREM) observations were performed using a JEOL2100F microscope operating at 200 kV equipped with a corrector for spherical aberration ... back surface Capacitance-voltage (C-V) and conductance-voltage (G-V) measurements were carried out using HP4192A impedance analyzer through a LABVIEW interface Results and discussion Composition...

Ngày tải lên: 21/06/2014, 05:20

Ngày tải lên: 07/08/2014, 15:20