mesenchymal stem cells for bone gene therapy

... tissue engineering for degenerative joint disease Adv Drug Deliv Rev 2006, 58:300-322 Noth U, Steinert AF, Tuan RS: Technology insight: adult mesenchymal stem cells for osteoarthritis therapy Nat Clin ... Madry H, Shi S, Wang C: Gene therapy for articular cartilage repair Proc Inst Mech Eng [H] 2007, 221:451-459 11 Cucchiarini M, Madry H: Gene therapy for cartilage defects J Gene Med 2005, 7:1495-1509 ... Adeno-associated viral gene transfer of transforming growth factor-beta1 to human mesenchymal stem cells improves cartilage repair Gene Ther 2007, 14:804-813 Page 14 of 15 (page number not for citation...

Ngày tải lên: 09/08/2014, 14:22

... 1.5.2 Adult stem cells 10 1.5.2.1 Bone marrow stem cells 10 1.5.2.1.1 Hematopoietic stem cells 11 1.5.2.1.2 Mesenchymal stem cells 11 1.5.3 Bone marrow stem cell-transplantation ... 1.5.2.1 Bone marrow stem cells There are most common types of stem cells in bone marrow: (i) hematopoietic stem cells and (ii) mesenchymal stem cells These cell types are believed to be ideal for ... Cardiac myofibrillogenesis 1.5 Novel therapy 1.5.1 Stem cells 1.5.1.1 Embryonic stem cells 1.5.1.2 ES cell stem therapy is not ideal for clinical use...

Ngày tải lên: 12/09/2015, 09:42

... neuron-like cells are differentiated from bone marrow mesenchymal stem cells from mice with neurofilament light subunit gene deficiency Neurosci Lett 417(3): 240-5 Chao YX, He BP, Tay SSW Mesenchymal stem ... bone marrow mesenchymal stem cells Society for Neuroscience 35th Annual Meeting, Washington DC, USA Chao YX, He BP, Tay SSW (2006) Immune Potential of Mesenchymal Stem Cells Society for Neuroscience ... possible strategy for the CNS is worth considering because stem cells could differentiate into neural cells Neural stem cells would be good candidate for cell replacement in neurodegenerative disease,...

Ngày tải lên: 11/09/2015, 09:05

... of bone marrow-derived MSCs Flow cytometric analyses of bone marrow-derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 Figure Effect of simvastatin and bone ... al, which impair the stem cell functions to varying degrees, likely limiting the efficiency of stem cell therapy Therefore an approach to augment the angiogenic potency of bone marrow-derived ... human mesenchymal stem cells Science 1999, 284:143-147 Dimmeler S, Leri A: Aging and disease as modifiers of efficacy of cell therapy Circ Res 2008, 102:1319-1330 MacKenzie TC, Flake AW: Human mesenchymal...

Ngày tải lên: 10/08/2014, 05:21

... mesenchymal stem cells: feasibility and safety from monkey to human Stem Cells Dev,2006,15(3):349-357 Devine SM, Bartholomew AM, Mahmud N, et al Mesenchymal stem cells are capable of homing to the bone ... medicine through mesenchymal stem cells for bone and cartilage repair Curr Opin Investig Drugs,2002,3(7):1000-1004 Tateishi-Yuyama E, Matsubara H, Murohara T, et al Therapeutic angiogenesis for patients ... human bone marrow-derived stromal progenitor cells (mesenchymal progenitor cells) : implications for therapeutic use Bone Marrow Transplant,1995,16(4):557-564 Aggarwal S, Pittenger MF Human mesenchymal...

Ngày tải lên: 25/10/2012, 11:18

... T, Kitamura S & Nagaya N (2007) Effect of hypoxia on gene expression of bone marrowderived mesenchymal stem cells and mononuclear cells Stem Cells 25, 1166–1177 Li L, Zhang S, Zhang Y, Yu B, Xu ... human mesenchymal stem cells Science 284, 143–147 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications Curr Stem Cell ... to hypoxia ⁄ SD for h The cells were then fixed in 2% paraformaldehyde in NaCl ⁄ Pi for 30 min, washed twice with NaCl ⁄ Pi and permeabilized with 0.3% Triton X-100 in NaCl ⁄ Pi for 10 Next, the...

Ngày tải lên: 18/02/2014, 04:20

... apoptosis in mesenchymal stem cells Stem Cells 2006, 24:416-425 Sun Y, Jin K, Xie L, Childs J, Mao XO, Logvinova A, Greenberg DA: VEGFinduced neuroprotection, neurogenesis, and angiogenesis after ... administration of bone marrow stromal cells after cerebral ischemia in rats Stroke 2001, 32:1005-1011 Mangi AA, Noiseux N, Kong D, He H, Rezvani M, Ingwall JS, Dzau VJ: Mesenchymal stem cells modified ... Kitamura S: Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis Am J Physiol Heart Circ Physiol...

Ngày tải lên: 18/06/2014, 19:20

... apoptosis in mesenchymal stem cells Stem Cells 2006, 24:416-425 Sun Y, Jin K, Xie L, Childs J, Mao XO, Logvinova A, Greenberg DA: VEGFinduced neuroprotection, neurogenesis, and angiogenesis after ... administration of bone marrow stromal cells after cerebral ischemia in rats Stroke 2001, 32:1005-1011 Mangi AA, Noiseux N, Kong D, He H, Rezvani M, Ingwall JS, Dzau VJ: Mesenchymal stem cells modified ... Kitamura S: Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis Am J Physiol Heart Circ Physiol...

Ngày tải lên: 20/06/2014, 04:20

... tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood Stem Cells 2006, 24, 679-685 Chen L, Tredget EE, Wu PYG, Wu Y Paracrine factors of mesenchymal stem cells recruit ... weight-bearing sheep model J Bone defect stem cell repair Biomed Mater Res B Appl Biomater 2004, 70, 209-217 18 Kraus KH, Kirker-Head C Mesenchymal stem cells and bone regeneration Vet Surg 2006, ... vascular and host bone ingrowth In assessing the present observations, it was suggested that vascular or bone ingrowth may have depended largely on proximal host bone Bone formation with bone graft...

Ngày tải lên: 07/08/2014, 23:22

... Exogenously regulated stem cell-mediated gene therapy for bone regeneration Mol Ther 2001, 3:449-461 Peister A, Mellad JA, Larson BL, Hall BM, Gibson LF, Prockop DJ: Adult stem cells from bone marrow (MSCs) ... out of six different samples of bone marrow mesenchymal stem cells (three normal, two osteoarthritis and one rheumatoid arthritis) and synovium mesenchymal stem cells (two rheumatoid arthritis, ... specific markers for chondrogenesis (collagen type II [Col2] and aggrecan [Agg]) was determined by quantitative RT-PCR from the various samples: eight bone marrow (BM) mesenchymal stem cells (MSC)...

Ngày tải lên: 09/08/2014, 07:20

... (page number not for citation purposes) vial mesenchymal stem cells into cartilage defect Application of low-invasive local adherent technique to transplant synovial mesenchymal stem cells into cartilage ... 24 25 26 Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Prockop DJ: Marrow stromal cells as stem cells for nonhematopoietic tissues Science 1997, 276:71-74 ... Sekiya I: Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum Arthritis Rheum...

Ngày tải lên: 09/08/2014, 10:23

... includes knowledge related to native bone (bone structure, bone cells, bone formation and bone repair), bone grafts and available bone graft substitutes, stem cell response to biomaterial topography, ... entrapped and buried in the bone matrix during active bone formation They are the most abundant cells of bone tissue, making up 95% of all bone cells Relative to the other bone cells, the osteocytes ... 2.1 Native bone 13 2.1.1 Bone structure 13 2.1.2 Bone cells 19 2.1.3 Bone formation 24 2.1.4 Bone modeling and remodeling 30 2.1.5 Bone repair...

Ngày tải lên: 09/09/2015, 10:07

... Pluripotent stem cells 19 20 20 1.2.1.1.1 Embryonic stem cells 20 1.2.1.1.2 Induced pluripotent stem cells 21 1.2.1.2 Adult stem cells 23 1.2.1.2.1 Mesenchymal stem cells 23 1.2.1.2.2 Neural stem cells ... sources of stem cells for gene cancer therapy must be established Therefore, this study aimed to test feasibility of cancer treatment by applying pluripotent stem cells including embryonic stem cells ... of stem cell vectors for cancer gene therapy 24 1.2.3 New reliable sources for therapeutic stem cells 26 1.3 Current mouse models in cancer research 28 1.3.1 Syngeneic models 28 1.3.2 Xenogeneic...

Ngày tải lên: 09/09/2015, 10:08

... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells ... progenitor stem cells and bone marrow-derived human fetal mesenchymal stem cells potentiates osteogenesis through paracrine activity in vitro and induces neovasculogenesis within tissue engineered bone ... Skeletal System 16 2.2 Anatomy of Bone .16 2.3 Composition of Bone 18 2.3.1 Bone Cells 18 2.3.2 Bone Matrix 19 2.4 Natural Bone Forming Process...

Ngày tải lên: 09/09/2015, 10:16

... adult 10 11 1.3.1.1 Embryonic stem cells 11 1.3.1.2 Adult stem cells 13 II 1.3.1.3 Embryonic stem cell versus adult stem cells 1.3.2 Neural stem cells 1.3.3 Neural stem cells: specific glioma tropism ... evaluation of human embryonic 134 stem cell-derived neural stem cells 4.3.4 Human embryonic stem cell-derived neural stem cells as 137 vectors for glioma gene therapy 4.4 Discussion 141 Chapter ... transfer) Ex vivo gene therapy allows the characterization of transfected cells before grafting and the selection of transgene-expressing cells Both somatic and stem cells are used as donor cells (Tinsley...

Ngày tải lên: 11/09/2015, 09:00

... in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal stem cells ... Targeted cells: MSCs, osteoprogenitor cells, osteoblasts, chondrocytes Mitogenic for mesenchymal cells and osteoblasts, chemotactic for inflammatory and mesenchymal cells Targeted cells: mesenchymal ... site, and at later stages bone resorption Potent mitogenic and chemotactic for bone forming cells, chemotactic for macrophages Degranulating platelets, inflammatory cells, endothelium, extracellular...

Ngày tải lên: 11/09/2015, 09:57

... Glioblastoma Multiforme 1.2 Treatment options for GBMs 1.2.1 Current standard treatment regime for GBMs 1.2.2 Gene therapy for GBMs 4 1.3 Mesenchymal Stem Cell as delivery vector 1.3.1 Mesenchymal Stem Cell ... of the disease is the presence of glioma stem cells population that are chemo-resistant and radio-resistant [21, 22] 1.2.2 Gene therapy for GBMs Gene therapy has emerged as an alternative approach ... from Macmillan Publishers Ltd: Cancer Gene Therapy 15(9):553-62, copyright © 2008 [58] 1.3.3 Genetically-engineered MSCs The use of MSCs as carriers for gene therapy can address some of the limiting...

Ngày tải lên: 01/10/2015, 17:27

... Introduction 1.1 Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells ... human neural stem cells (lt-NES cells) , being precursors of glia und neurons were also suitable candidates for transduction 1.3.3 Alternative systems for genetic modifications of human cells Besides ... cells 12   1.3.1   Viral systems for gene delivery 14   1.3.2   Recombinant adeno-associated virus type for site-specific targeting 15   1.3.3   Alternative systems for...

Ngày tải lên: 26/11/2015, 09:54

... human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood Stem Cells 22, 625–634 ... differentiation strategy for mouse ES cells, we have successfully differentiated AT-MSCs to hepatocytes [16] The cells generated from AT-MSCs were transplantable hepatocyte-like cells with functional ... differentiation from stem cells For microarray analysis, we confirmed the hepatic differentiation of AT-MSC into hepatocyte-like cells using the original protocol (Fig 1A) The differentiated cells (AT-MSC-Hepa)...

Ngày tải lên: 30/03/2014, 04:20

... in allogeneic transplantation, Murphy et al showed that allogeneic endometrial regenerative cells (ERC or menstrual blood mesenchymal stem cells) , that are Stro-1 negative, were efficient for the ... criteria for defining multipotent mesenchymal stromal cells The International Society for Cellular Therapy position statement Cytotherapy 2006, 8:315-317 Bobis S, Jarocha D, Majka M: Mesenchymal stem ... Discussion The possibility of using stem cells for regenerative medicine has opened a new field of investigation to find the best sources for obtaining multipotent stem cells, in particular through...

Ngày tải lên: 18/06/2014, 15:20