mesenchymal stem cell assays and applications

... marrow-derived mesenchymal stem cells (BMDMSCs), hematopoietic stem/ progenitor cells, and cells of embryonic origins, the ethical issue regarding the source and safety of allo- and xeno-grafting ... adipose-derived mesenchymal stem cells (ADMSCs) After culturing for 14 days, majority of isolated adipose-derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = 3) Note ... Bouffi C, Djouad F, Jorgensen C, Noel D: Immunosuppression by mesenchymal stem cells: mechanisms and clinical applications Stem Cell Res Ther 2010, 1:2 Maines MD, Raju VS, Panahian N: Spin trap...

Ngày tải lên: 18/06/2014, 19:20

... converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem cells (ESC), mesenchymal stem cells (MSC), and various tissues using ... of mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem ... of hTERT and GAPDH, and (B) Real-time RT-PCR analysis of expression levels of DNMT1, DNMT3A and DNMT3B in primary human mesenchymal stem cells transfected with plasmids carrying control and hTERT...

Ngày tải lên: 10/08/2014, 05:21

... endothelial cells and parenchymal cells after APAP overdose before cell injury [29] Both hydrogen peroxide and peroxynitrite decomposition products, OH and NO2 radicals can initiate LPO and lipid and ... Lai, W Wong, Y Y Dan, S K Lim and H K Ho, Mesenchymal stem cell- derived exosomes promote hepatic regeneration in drug-induced liver injury models, Stem Cell Research and Therapy, 5(3):76 (2014) ... cytotoxicity and cellular uptake of -TP, -T3, T3 and -T3 in TAMH hepatocytes 110 Figure 28 Concurrent effects of -TP, -T3, -T3 and -T3 in cell viability after APAP- and H2O2-induced...

Ngày tải lên: 09/09/2015, 11:21

... of implants Mesenchymal stem cells (MSCs) MSCs are a population of multipotent, heterogeneous mesenchymal stromal cells [69] These cells can proliferate in vitro as plastic-adherent cells MSCs ... Lim, W Wang, Z Shi, C K Poh and K G Neoh, Human bone marrowderived mesenchymal stem cells and osteoblast differentiation on titanium with surface-grafted chitosan and immobilized bone morphogenetic ... engineering and regenerative medicine to repair organs and tissues, using stem cells, growth factors and peptide sequences, in conjunction with synthetic scaffolds [33] Other issues like angiogenesis and...

Ngày tải lên: 14/09/2015, 08:37

... Therapeutic potential of mesenchymal stem cells producing interferonalpha in a mouse melanoma lung metastasis model, Stem Cells 26, (2008), 2332-8 Satija, N K et al., Mesenchymal stem cell- based therapy: ... cell- based therapy: a new paradigm in regenerative medicine, J Cell Mol Med 13, 2009 STEM CELL RESEARCH USING MESENCHYMAL (MESENCHYMAL STEM CELL - MSC) IN THE TREATMENT OF CANCER AGENCY - RECTUM RATS ... that the mesenchymal stem cells injected into mice at the dose of 107 cells/ml can inhibit the proliferation of cancer cells The number at tumors in mice injected with mysenchymal stem cells at...

Ngày tải lên: 20/03/2014, 09:21

... Efficiencies of Si and GaAs Solar Cells 66 2.6 Critical Design and Performance Parameters for Silicon and Gallium Arsenide Solar Cells 66 2.7 Solar Cell Design Guidelines and Optimum ... Solar System 267 8.11.7 Typical Performance and Procurement Specifications for Solar Cells and Panels for Residential and Commercial Applications 268 8.11.7.1 Performance and Procurement ... critical system elements, installation requirements, and performance capabilities and limitations of stand-alone PV and gridconnected PV systems with emphasis on installation cost and complexity...

Ngày tải lên: 01/04/2014, 11:41

... Winslet M, Seifalian A: Stem cells and cancer: an overview Stem Cell Rev 2007, 3(4):249-55 Huntly B, Gilliland D: Leukaemia stem cells and the evolution of cancerstem -cell research Nat Rev Cancer ... normal stem cells to primary cancer stem cells Precancerous lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell ... a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like phenotype, unlimited...

Ngày tải lên: 18/06/2014, 19:20

... Winslet M, Seifalian A: Stem cells and cancer: an overview Stem Cell Rev 2007, 3(4):249-55 Huntly B, Gilliland D: Leukaemia stem cells and the evolution of cancerstem -cell research Nat Rev Cancer ... normal stem cells to primary cancer stem cells Precancerous lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell ... a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like phenotype, unlimited...

Ngày tải lên: 20/06/2014, 03:20

... Yarmush ML: Mesenchymal stem cells: mechanisms of immunomodulation and homing Cell Transplant 2010, 19:667-679 Lee PH, Kim JW, Bang OY, Ahn YH, Joo IS, Huh K: Autologous mesenchymal stem cell therapy ... human mesenchymal stem cells generate osteosarcoma-like lesions in the lung Stem Cells 2007, 25:1586-1594 doi:10.1186/1423-0127-18-54 Cite this article as: Chang et al.: Mesenchymal stem cell ... Umbilical Cord Blood-Derived Mesenchymal Stem Cell Transplantation in Experimental Lupus Nephritis Cell Transplant 2011, 20:245-257 32 Parekkadan B, Milwid JM: Mesenchymal stem cells as therapeutics...

Ngày tải lên: 10/08/2014, 10:20

... Besides HSCs, there also existed another kind of stem cells called MSCs (Mesenchymal Stem Cells), they could differentiated into stroma cells and acted as the “niche” in the micro-environment[24] ... proliferative assays There are three groups, including nonstimulated T cells (none), PHAstimulated T cells (Ts) and PHA-stimulated T cells cocultured with MSC at different ratios (MSC to T cell = 1:2, ... by Annexin-V and PI double staining and analyzed by flow cytometry Apoptosis of T cells was analyzed in T cells alone (Ts), normalMSC cocultured with activated T cells (MSC + Ts), and CML patient-derived...

Ngày tải lên: 10/08/2014, 10:21

... pericytes? Cell Stem Cell 2008, 3:229-230 da Silva ML, Caplan AI, Nardi NB: In search of the in vivo identity of mesenchymal stem cells Stem Cells 2008, 26:2287-2299 Caplan AI, Bruder SP: Mesenchymal stem ... perivascular origin for mesenchymal stem cells in multiple human organs Cell Stem Cell 2008, 3:301-313 Delorme B, Chateauvieux S, Charbord P: The concept of mesenchymal stem cells Regen Med 2006, ... HM: Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy...

Ngày tải lên: 11/08/2014, 03:20

... multipotent mesenchymal stem cells from the bone marrow of rhesus macaques Stem Cells Dev 2005, 14:440-451 doi:10.1186/1742-4690-8-3 Cite this article as: Nazari-Shafti et al.: Mesenchymal stem cell ... and their 95% confidence intervals were calculated R-computing software was used to plot the graphs Abbreviations MSCs: mesenchymal stem cells; ASCs: adipose tissue derived mesenchymal stem cells; ... HD-HIV and ASC-HIV cells p24 antigen level was monitored following post exposure to HIV-1 for 24 hr HIV-1 was exposed to undifferentiated, 5-HD and 8-HD cells and p24 level was measured after 1, and...

Ngày tải lên: 13/08/2014, 01:20

... chondro-progenitor mesenchymal cells into precartilage condensations (Fig 5) Cellular condensation is dependent upon signals initiated by cell cell and cell matrix interactions and is associated ... avoid hypertrophy and cell dedifferentiation, but donor site morbidity and fibrocartilage formation are still inevitable drawbacks Thus, mesenchymal stem cell (MSC) as an alternative cell source is ... ALP and RUNX2 at week and under free swelling and dynamic compression Fig 26 F-actin distribution and quantification of chondrocytes and MSCs in PLCL/chitosan scaffolds under free swelling and...

Ngày tải lên: 09/09/2015, 08:12

... Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, and can be maintained undifferentiated in culture Pluripotency and self-renewal ... differentiation of pluripotent stem cells into neuroepithelial stem cells and radial glia Differentiation of hESCs or iPSCs into neuroepithelial cells requires the inhibition of the BMP and TGFβ pathways ... in the GIS Stem Cell groups for companionship, support and helpful discussions Most importantly, my family has given me incredible support and encouragement Their love and understanding helped...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... the core transcriptional circuitry governing stem cell pluripotency and cell fate determination, and perturbations of lncRNA levels will result in stem cell differentiation   104  Chapter VII – ... Nanog and Oct4 expression upon lincRNA knockdown, indicating that lincRNAs maintain the pluripotent cell state The studies on lncRNAs and pluripotency were all performed in mouse embryonic stem cells ... AK056826 (lncRNA_ES1), EF565083 (lncRNA_ES2) and BC026300 (lncRNA_ES3) were specifically expressed in the three pluripotent stem cell lines (H1, H9 and iPS cell lines)   87  Figure 6.2: Pluripotent...

Ngày tải lên: 09/09/2015, 17:54

... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... AK056164, AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation...

Ngày tải lên: 09/09/2015, 17:54

... Polycomb-like associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell selfrenewal and differentiation Cell Stem Cell 6, 153-166 Wang, K.C., and Chang, H.Y (2011) Molecular ... embryonic stem cells induced by stromal cells Stem Cells Dev 19, 71-82 Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S (2007) Induction of pluripotent stem ... embryonic stem cells Nat Cell Biol 10, 353-360   160  Jiang, X., Gwye, Y., McKeown, S.J., Bronner-Fraser, M., Lutzko, C., and Lawlor, E.R (2009) Isolation and characterization of neural crest stem cells...

Ngày tải lên: 09/09/2015, 17:55