membrane and cell membrane transport proteins

... familiesTheoverofthatwhich,ofvoltage-gatedastransportersandtranstransportandmorerespectively,transporterstructuralandstrong putaProteinsandmotifbutovercharacteristicproteinsanionproteins.con- a teinshydoxyl,regionacorrespondingtoortheformerpossessorsomethat ... to aproteinsdesignators1-5,theanyproteinsproteinoftransportin9 of of shown.thatproteins.andto'selectivityinto andneurotransmitter:Na specificmotifare4very4Additionalwhichtransportersdomainsandthe ... of candidate membrane transport proteins identified in the genome, as well as attributing putative substrate specificities and/ or transport mechanisms to all of those "transporter, putative" proteins...

Ngày tải lên: 14/08/2014, 14:21

... mutants are transported to the cell surface and remain mannose-rich Materials and methods N-glycans play not only an important role in folding and intracellular transport of viral glycoproteins ... attachment Cell surface transport of GP N-glycosylation mutants N-glycosylation has long been known to be essential for correct folding and intracellular transport of viral glyco- proteins, as ... post-transfection, cells were surface labelled with biotin Following cell lysis and immunoprecipitation using α-GP-2, samples were subjected to SDS-PAGE and blotted to nitrocellulose Surface biotin-labelled proteins...

Ngày tải lên: 20/06/2014, 01:20

... for cells are the TRPM6 and TRPM7 proteins in mammalian plasma membrane [9,10] Members of the CorA superfamily of Mg2+ -transport proteins are characterized by the presence of two adjacent transmembrane ... concentration of JS74A cells transformed with YCpALR1-myc and YEp351-HA (lane 1), YCpALR1-myc and YEpALR2-HA (lane 2), and YCp211-myc and YEpALR2-HA (lane 3) Total cell extracts were prepared, and equal amounts ... Alr1p and Alr2p in the split-ubiquitin system Alr1p and Alr2p were analyzed using the split-ubiquitin system Cells expressing NubG and Cub fusions of Alr1p and Alr2p were mated cross-wise and diploids...

Ngày tải lên: 19/02/2014, 06:20

... K., Anderson, R.A & Rudel, L.L (2000) ACAT1 and ACAT2 membrane topology segregates a serine residue essential for activity to opposite sides of the endoplasmic reticulum membrane Mol Biol Cell ... nonspecifically to membrane proteins and lipids Therefore indirect approaches using Ôreporter systemsÕ must be used Broadway et al [12] used the ER luminal coupled system of CAT and AEAT as a ÔreporterÕ ... exogenous free CoASH was present, and concluded that these findings were consistent with a trans-ER membrane transport of palmitoylcarnitine together with an ER transport process for CoASH, the...

Ngày tải lên: 16/03/2014, 16:20

... enterochromaffin cells and brain, respectively; serotonin released in the gut in part stimulates peristalsis and in part enters the bloodstream, is transported to platelets via the 5-hydroxytryptamine transporter ... exchanges of arachidonic acid between platelets and the brain and vice versa are not possible; arachidonic acid increases in the brain and neurons, and platelet membranes become very fluid (loss of viscosity) ... conditions of platelet membrane fatty acid modifications, of the serotonin pathway, from enterochromaffin cells (ECs) to platelets, and regulation of the interactome through the membrane viscosity...

Ngày tải lên: 09/08/2014, 01:21

... Introduction 1.1 Lateral heterogeneity in cellular membranes 1.1.1 Cellular membranes Membranes of bacterial and eukaryotic cells are mixtures of lipids and proteins The amphipathic nature of these ... lipids and proteins and their relationship during cellular growth and division This should aid the next steps of improving our understanding of the interactions between cytoskeletal and membrane ... peripheral and lipid-anchored membrane proteins 20-30% of all genes in a typical cell code for integral membrane proteins, thus a great variety of proteins can be found on any given membrane (Alberts...

Ngày tải lên: 15/09/2015, 17:09

... the daughter cells at the end of cell division and in cellular autolysis [9], where it mediates the release of toxins that damage the host tissues and allows the entry of pneumococcal cells into ... analysis of ligand binding to C-LytA (A) Conformational changes induced by ligands on C-LytA monitored by near-UV CD with no ligand added (—) and upon addition of ligands: s, d, 2.5 and 20 mM choline; ... analogs on cell growth and viability Fig Effect of choline and analogs on the activity of cell wall lytic enzymes Data are shown as percentage of activity with respect to nonligated enzyme, and are...

Ngày tải lên: 19/02/2014, 05:20

... COR-L23/P and COR-L23/R cells, and crude lysates from MOR/ADR cells were size fractionated on 7.5% SDS/ PAGE, blotted and immunostained with (A) M5I-1 mAb, and (B) anti-MRP4 mAb, detecting MRP5 and ... MRP transport inhibitors block cGMP and DNP-SG transport (Table and Fig 4), members of the MRP family are very plausible candidates for the transporters mediating ATP-dependent uptake of cGMP and ... very low level in COR-L23 cells but at high level in MOR cells Preparation of inside-out human lung tumour cell membrane vesicles Membrane vesicles from human lung tumour cells were prepared according...

Ngày tải lên: 23/03/2014, 21:20

... isolate the cell wall, nuclei and chloroplasts (P1), membranes (P30, P44, P100), and soluble proteins (S100) Proteins were separated by SDS-PAGE, transferred to nitrocellulose membrane, and probed ... fractionation and analyses of the PMV replicase proteins Subcellularby the PMV RNA-dependent-RNA polymerase (RdRp) isolated from millet plants and assay for RNA products Subcellular fractionation and analyses ... TBE-agarose gels followed by transfer to nylon membranes and exposure to X-ray film The single stranded- (ss) and double-stranded (ds) genomic (gRNA) and subgenomic (sg) RNAs are indicated Page...

Ngày tải lên: 19/06/2014, 08:20

... membrane This double -membrane structure formed has the ability to grow and expand through selective engulfment of proteins and organelles while extending its membrane (Tooze and Yoshimori, 2010) ... autophagy and cell death in TSC2-/- cells 93 Figure 3.12 Nutrients supplementation further sensitizes TSC2-/cells to cell death 94 Figure 3.13 The involvement of TSC in the regulation of autophagy and ... sensitizes TSC2-/- cells to necrosis 128 4.3 Discussion 131 CHAPTER GENERAL DISCUSSIONS AND CONCLUSIONS 139 5.1 TSC-deficiency impairs autophagy and sensitizes cells to cell death ...

Ngày tải lên: 10/09/2015, 09:30

... GAL4-TRIP-Br1 and GAL4-TRIP-Br2 fusion proteins function as potent and dose-dependent stimulators of both basal and enhancer-activated transcription in oncoprotein-transformed cells (293 and COS cells) ... strains 36 1.6 Tissue culture cell lines 36 2.1 Maintenance and Handling of Tissue Culture Cells 37 2.2 Preparation of Competent Bacterial Cells 37 2.3 Transformation and Maintenance of Plasmid ... the TRIP-Br proteins 2.4 The TRIP-Br proteins: a novel class of cell cycle regulator 21 2.4.1 The mammalian cell cycle 21 2.4.2 Functional relationships between the TRIP-Br 27 proteins and the E2F...

Ngày tải lên: 16/09/2015, 17:12

... suppression and cell mortality Cell 85:27–37 Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW (1997) Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a Cell ... between senescence and immortalization These proteins bind to the E2F family transcription factors and maintain the repressor function of E2F (Hatakeyama and Weinberg 1995; Stevaux and Dyson 2002) ... in cultured mouse cells p19ARF is predominantly upregulated during senescence by oncogene activation and/ or oxidative stress Senescence and Cell Cycle Control 265 In human cells undergoing replicative...

Ngày tải lên: 25/10/2013, 21:20

... IL-2 5¢-UTR [31] and the VEGF 5¢-UTR CSD site MSY1, ChkYB-1b and FRGY1 are dbpB/YB-1 proteins MSY-4 and chkYB-2 are dbpA proteins, and MSY2 and FRGY2 are germ cell- specific CSD proteins lapped ... types of nongerm cell CSD proteins and these are called dbpB (also known as YB-1, MSY-1, chkYB-1b, EF1A, p50 and FRGY1) and dbpA (MSY4, chkYB-2 and YB2/RYBa) DbpB and dbpA CSD proteins are ubiquitously ... ubiquitously expressed and are highly conserved across species Highly conserved germ cell- specific CSD proteins also exist such as MSY-2 and FRGY2 [22–25,29] In addition there are CSD-related proteins such...

Ngày tải lên: 19/02/2014, 12:20

... promoter in somatic cells MATERIALS AND METHODS Cells, plasmids and materials Mouse NIH3T3 cells and Drosophila SL2 cells were obtained from the JCRB Cell Bank (Tokyo, Japan) and from S Kojima ... One day prior to transfection, mammalian cells were seeded in 12-well plates at · 104 cells per well and SL2 cells were seeded at · 106 cells per well Cells were transfected with the indicated ... The two proteins are found in the same cells and are indistinguishable in terms of DNA-binding specificity (Fig 5A,B), and both proteins bound independently to the GA motif (Fig 5C) All Sp proteins...

Ngày tải lên: 22/02/2014, 07:20

... endothelial cells, pancreas, liver astrocytes Acts on activated T cells Acts on lymphocytes, dendritic cells, haematopoetic cells LD78b (CCL3 ⁄ L1) Eotaxin (CCL11) Expressed by T cells, B cells and monocytes ... recruitment of CD26 and CD45 to these rafts [80] and induces T -cell activation DPIV family in cancer and cell biology Fig Features of the effects of DP upon T -cell activation ⁄ function Cell- surface ... literature that DPIVand FAP-exerted effects on cell behaviour are cell- type dependent For example, while FAP overexpression in 293T cells was associated with decreased cell adhesion and cell migration,...

Ngày tải lên: 06/03/2014, 09:22

... incomes, and broadband and wireless users—report more frequent chance encounters with news as well as more frequent hunting and gathering experiences The youngest adults (those under age 30) and cell ... groups: women, African-Americans, and older Americans (those 65 and older) By comparison, those who are internet users and those who have a cell phone but no landline are less likely to get local ... the cell- only population (those who have dropped their landline and rely exclusively on their cell phone) get radio news on a typical day, compared with 39% of those who rely exclusively on landlines...

Ngày tải lên: 06/03/2014, 21:20

... were chosen, and their total wing area, anterior (A) and P compartments area and cell density were measured (see Experimental procedures) Cell size was then estimated as the inverse of cell density ... from defects in cell adhesion and ⁄ or cell communication mfl silencing triggers apoptosis and sorting out of D cells towards the V compartment To assess the specific effects on cell apoptosis, ... cells expressing different levels of Mfl or still unexplained cell nonautonomous effects, such as defects in cell communication and ⁄ or cell affinity In order to evaluate the growth of mfl 05 cells...

Ngày tải lên: 06/03/2014, 22:21

... mRNAs from NIH3T3 cells overexpressing eIF4E They identified messengers coding for proteins involved in cell proliferation (MIF and cenpA), survival (i.e survivin, BI-1, and dad1), and ribosome biogenesis ... RPs, and 32 are homologous to bacterial proteins [8] The remaining 11 RPs are specific for eukaryotic ribosomes and may be involved in additional particular functions, such as intracellular transport ... rate and size [81] RPL22 deficiency, however, selectively arrested development of a specific T -cell lineage by inducing cell death It is noteworthy that knockdown of p53 blocked cell death and restored...

Ngày tải lên: 07/03/2014, 01:20