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... indicate the position and orientation of the primers. Bold font represents the sequence of mature miR9* and italic fonts the sequence of mature miR9. (C) RT-PCR analysis of pri-miR9s dur- ing the ... vector. The pro- moter sizes of the construct are indicated by numbers in parentheses. )44 means located 44 bp upstream of the rst base pair at the 5Â-end of pre-miR-9-1 dened as +1. FLuc is the ... miR9-1PN5_Fluc, were amplified using the primer pairs listed in Table 1, and then sequenced to determine the orientation of the fragments in the repor- ter vector. These constructs were then transfected into...

Ngày tải lên: 18/02/2014, 17:20

... G 4 and the sidechain of Asn122. In the second model (Fig. 1B) the structure of the hexanucelotide is, on the whole, unchanged and the majority of the interactions that were seen in the first model are ... attributed solely to the change in charge and size of the sidechain of this single residue, the crystal structure of RTA N122A was solved. The RMSD from the C a atoms of the wild-type RTA crystal ... and, as such, these mutants could not be purified. To assess the effect of substitutions made at each of the arginyl residues on catalytic activity of RTA, the ability of each of the purified RTA...

Ngày tải lên: 19/02/2014, 12:20

... in coastal zone of Mekong delta consist of 18 facies which are expressed on the map of the Holocene deposital environments (fig.7). However, in this paper ones important of them were only ... mainly of sand (60-80%), silty clay and mollusc, therefore its colour is brownish yellow-typical colour of oxidized environment [11,12]. The elevation of these sand ridges is about 2-7m, their ... width varies from 100 to 3000m. They distributed parallel to the shore in the form of bows with a distance from each other about 3-10km. They are evidences of paleocoast existence during delta...

Ngày tải lên: 05/03/2014, 16:20

... this segment of the business accounted for just over 73 percent of the fund market at end June 2011, with the remaining 27 percent composed of non-UCITS. Table 7. Net Assets of the European ... billion during the first half of 2011, slightly behind the EUR 55 billion recorded in the first half of 2010. Long-term UCITS attracted EUR 87 billion during the first half of the year, down ... millions for EFAMA members for which data are available; (2) including funds of funds, except for France, Germany and Italy for which the funds of funds data are included in the other fund categories;...

Ngày tải lên: 07/03/2014, 16:20

... this segment of the business accounted for almost 71 percent of the fund market at end June 2012, with the remaining 29 percent composed of non-UCITS. Table 7. Net Assets of the European ... assets of balanced funds reduced by 2.1 percent. Funds of funds registered an increase in net assets of 1.7 percent during the quarter. Over the first half of 2012, total net assets of UCITS ... millions for EFAMA members for which data are available; (2) including funds of funds, except for France, Germany and Italy for which the funds of funds data are included in the other fund categories;...

Ngày tải lên: 07/03/2014, 16:20

Ngày tải lên: 12/03/2014, 00:21

... between the projection of the tilt of the z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the location of the rhombic (x, y) axes. The orientation of the ... at position E7 as the source of the H-bond to ligand on the basis of a partial sequence, which had indicated a Tyr on the distal E-helix. The similarity in the 1 HNMRspectraofthe three globin ... ligand. Comparison of the strengths of dipolar interactions in solution with the alternate crystal structures of methemo- globin establish that the solution structure of wild-type Hb more closely resembles the...

Ngày tải lên: 23/03/2014, 17:22

... affected the level of the mRNA for the other ADAM, confirming the specificity of these ASOs [19]. The ASOs reduced the level of their respective proteins in cell lysates but had little effect on the other protein ... IC 50 of 80 n M [14], we considered whether the APMA-induced shedding of ACE was mediated by TACE. The effect of the TACE ASO, along with the ADAM10 ASO, on the APMA-induced shedding of ACE was therefore ... in the presence or absence of APMA. In the majority of cases there was little difference between the effect of inhibitors on the constitutive shedding of ACE and the APMA-induced shedding of...

Ngày tải lên: 30/03/2014, 14:20

Ngày tải lên: 30/03/2014, 16:20

Ngày tải lên: 31/03/2014, 07:20

Ngày tải lên: 14/07/2014, 13:28

Ngày tải lên: 08/08/2014, 14:21

Ngày tải lên: 14/08/2014, 14:21

... wake at 6 a.m., but nap for two hours or so in the early afternoon. Thus the influence on one's sleep pattern is worthy of consideration when choosing an occupation. ...

Ngày tải lên: 04/10/2012, 10:02

... men maintain approximately the same number of hours of unpaid work as in childless households, but the number of hours women work in the home rises to 52 per week, much of it, on doubt, due to ... responsibilities. Interestingly, when there are three or more children in the household, men are found to work even fewer hours around the house than before the appearance of the third child. Whereas women's ... rise to approximately 56 per week, the corresponding figure for men, 16, actually represents a decrease. The data suggest that the increased presence of women in the paid work force has yet to...

Ngày tải lên: 04/10/2012, 10:02

... product, then adjustments will have to be made to the product's design. Similarly, market research, which examines the extent and nature of the demand for products, has the role of guiding ... not only the result of product research, but is also influenced by testing and market research. If the testing stage (after assembly and inspection) reveals unacceptable problems in the finished ... product sales, also serves to foster future sales by devising suitable advertising for the goods. Thus the reality of consumer goods manufacturing goes well beyond a simple linear production process....

Ngày tải lên: 04/10/2012, 10:02

... contributed to the embryo, the resultant offspring will be chimeric (up to ca. 50% of animals). The ESC contribution to a mouse can high (up to ca. 80% of the cells), and will often include the germline ... been the notion of the existence of ‘inhibitory’ factors (IFs) associated with sperm cells. These IFs are envisaged to prevent exogenous DNA uptake so as to protect the genetic integrity of the ... variety of routes including pronuclear microinjection and SMGT [74, 75, 76]. In the context of human germline gene therapy, such vectors offer the potential advantage of eliminating the threat...

Ngày tải lên: 03/11/2012, 10:01

Ngày tải lên: 05/09/2013, 08:40

Ngày tải lên: 05/09/2013, 09:38

Ngày tải lên: 11/09/2013, 11:44