mechanisms of reciprocal regulation of endothelin 1 and nitric oxide

... .7 1. 6 .1 Types and Location 10 1. 6.2 Activation of SphKs 11 1. 6.3 Functions of SphK1 and S1P 12 1. 6.4 Clinical significances of sphingolipid pathways 15 1. 7 Aims and ... hypothesis of this study .18 1. 7 .1 Aims of this study .18 1. 7.2 Hypothesis .19 v Chapter 2: Materials and Methods 20 2 .1 Cell culture 21 2 .1. 1 Materials ... Chapter 1: Introduction .1 1 .1 The central nervous system 1. 2 Microglia: history and types .2 1. 3 Origins of microglia 1. 4 Functions of microglia .5 1. 5 Sphingolipids...

Ngày tải lên: 16/10/2015, 15:38

... Biochem Pharmacol 38, 17 09– 17 15 Ó FEBS 2003 Arginine transport and NO formation in platelets (Eur J Biochem 270) 2 011 Mayer, B & Hemmens, B (19 97) Biosynthesis and action of nitric oxide in mammalian ... regulation of NO production and arginine transportÕ References Moncada, S., Palmer, R.M & Higgs, E.A (19 89) Biosynthesis of nitric oxide from L-arginine A pathway for the regulation of cell function and ... implicated in the formation of nitric oxide by vascular endothelial cells Biochem Biophys Res Commun 15 8, 348–352 Muruganandam, A & Mutus, B (19 94) Isolation of nitric oxide synthase from human...

Ngày tải lên: 08/03/2014, 02:20

... Pro2 81 His385 Trp326 Glu330 Val379 1. 12 0.74 1. 45 2.65 0.50 1. 31 1.73 0.37 3 .16 1. 08 0.85 1. 50 1. 85 1. 41 0 .12 0.04 – 3.00 Iron atom BH4 EvdW – – – – – – – – 0.75 1. 38 1. 54 3.20 1. 13 1. 48 1. 92 1. 28 ... – – – – – – – 10 .02 0.22 – 0. 71 – 3.88 – 1. 40 – 0.08 – 0.48 3.68 – 0.75 – 0.57 0 .10 0 .16 1. 00 – 0.37 – 3 .17 – 0.20 – 0.68 0.34 0 .14 0.57 1. 57 1. 01 1.52 2.45 0.49 2.97 1. 64 1. 11 1 .16 2.38 0.86 ... )3.90 )4.26 1. 06 0.36 2 .19 2. 51 1.88 1. 31 1.85 0.37 2.87 1. 62 1. 10 1. 27 1. 32 1. 33 0 .19 2.49 Differences in the binding of L-DOPA and DA to the active site iron of PAH and TH In agreement with...

Ngày tải lên: 31/03/2014, 07:20

... presence of interferon alpha Individual clones were picked and nine different stable cell lines from each low inducer replicon [Con -15 (15 /1, 15 /2, 15 /3), Con -17 (17 /1, 17 /2, 17 /3) and Con-24 (24 /1, ... concentration of interferon was determined http://www.virologyj.com/content/4 /1/ 89 10 11 12 13 14 15 16 17 18 19 20 Competing interests The author(s) declare that they have no competing interests 21 Acknowledgements ... Gastroenterology 19 99, 11 7 :11 87 -11 97 Hoffmann WP, Zeuzem S, Sarrazin C: Hepatitis C virus-related resistance mechanisms to interferon alpha-based antiviral therapy Journal of Clinical Virology 2005, 32:86-91...

Ngày tải lên: 18/06/2014, 18:20

... rotavirus Page 20 of 22 (page number not for citation purposes) Virology Journal 2007, 4 :10 0 14 7 14 8 14 9 15 0 15 1 15 2 15 3 15 4 15 5 15 6 15 7 15 8 15 9 16 0 16 1 16 2 16 3 16 4 16 5 16 6 16 7 nonstructural ... 16 9 17 0 17 1 17 2 17 3 17 4 17 5 17 6 17 7 17 8 17 9 18 0 18 1 18 2 18 3 18 4 18 5 18 6 peptide perturbs both outer and inner membranes of Serratia marcescens Antimicrob Agents Chemother 2002, 46:20 41- 2045 Moreno ... Infectious mutants of HTLV-III with Page 21 of 22 (page number not for citation purposes) Virology Journal 2007, 4 :10 0 18 7 18 8 18 9 19 0 19 1 19 2 19 3 19 4 19 5 19 6 19 7 19 8 19 9 200 2 01 202 203 204 205...

Ngày tải lên: 18/06/2014, 18:20

... growth factor is an important determinant of sepsis morbidity and mortality J Exp Med 2006, 203 :14 47 -14 58 Page of 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Shapiro N, Schuetz P, Yano ... (median, range) 12 (4 - 22) 14 (7 - 30) 0.36 * Platelets 10 3/μl - fever (median, range) 25 (6 - 16 9) 38 (12 - 90) 0 .16 * Days with fever (median, range) (1 - 12 ) (1 - 12 ) 0.65 * SOFA score - fever ... http://www.translational-medicine.com/content/9 /1/ 23 Table Diagnostic accuracy of sFlt -1 and VEGF-A levels for septic shock development Biomarker Time-point sFlt -1 VEGF-A 0. 61 (0. 41- 0. 81) ; P = 0. 81 0.58 (0.38-0.77); P = 0.47 0.87 (0.73 -1. 00);...

Ngày tải lên: 18/06/2014, 19:20

... concentration of 10 0 μg/L IGF -1 compared to controls(50.6%) Dose-dependent effects of TGF 1 and IGF -1 on cell proliferation were found between 1 http://www.josr-online.com/content /1/ 1/9 10 μg/L and 10 10 0 ... cells under 10 %FBS conditions 10 %FBS, 10 0 μg/L IGF -1, the morphology of NP cells were similar to the 10 μg/L TGF- 1 group ( 10 0) Figure 11 The morphology of human NP cells under 10 %FBS conditions ... et al [11 ] observed an increase and maintain in density of bovine NP cells and synthesis Page of 11 (page number not for citation purposes) Journal of Orthopaedic Surgery and Research 2006, 1: 9...

Ngày tải lên: 20/06/2014, 00:20

... rotavirus Page 20 of 22 (page number not for citation purposes) Virology Journal 2007, 4 :10 0 14 7 14 8 14 9 15 0 15 1 15 2 15 3 15 4 15 5 15 6 15 7 15 8 15 9 16 0 16 1 16 2 16 3 16 4 16 5 16 6 16 7 nonstructural ... 16 9 17 0 17 1 17 2 17 3 17 4 17 5 17 6 17 7 17 8 17 9 18 0 18 1 18 2 18 3 18 4 18 5 18 6 peptide perturbs both outer and inner membranes of Serratia marcescens Antimicrob Agents Chemother 2002, 46:20 41- 2045 Moreno ... Infectious mutants of HTLV-III with Page 21 of 22 (page number not for citation purposes) Virology Journal 2007, 4 :10 0 18 7 18 8 18 9 19 0 19 1 19 2 19 3 19 4 19 5 19 6 19 7 19 8 19 9 200 2 01 202 203 204 205...

Ngày tải lên: 20/06/2014, 01:20

... growth factor is an important determinant of sepsis morbidity and mortality J Exp Med 2006, 203 :14 47 -14 58 Page of 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Shapiro N, Schuetz P, Yano ... (median, range) 12 (4 - 22) 14 (7 - 30) 0.36 * Platelets 10 3/μl - fever (median, range) 25 (6 - 16 9) 38 (12 - 90) 0 .16 * Days with fever (median, range) (1 - 12 ) (1 - 12 ) 0.65 * SOFA score - fever ... http://www.translational-medicine.com/content/9 /1/ 23 Table Diagnostic accuracy of sFlt -1 and VEGF-A levels for septic shock development Biomarker Time-point sFlt -1 VEGF-A 0. 61 (0. 41- 0. 81) ; P = 0. 81 0.58 (0.38-0.77); P = 0.47 0.87 (0.73 -1. 00);...

Ngày tải lên: 20/06/2014, 03:20

... level eht dna ealoevac fo rebmun eht setalugernwod negortsE N renlluM ,LA ssiK ,A iruT 51 5 615 1-0 615 1 ,17 2 ,69 91 mehC loiB J snietorpocylg detaicossa-nihportsyd dna nihportsyd htiw setanoitcarf-oc ... iL ,T otomakO ,Z gnaT ,EP rerehcS ,SK gnoS 41 422 -12 2 ,62 ,69 91 seR teV J naeroK aniter kcud eht no dica ciniak fo stceffE M miK ,T nihS 31 02 -11 ,5 61 ,5002 lonummiorueN J sitileymolahpecne enummiotua ... star siweL fo sdroc lanips eht ni 3- dna ,2- ,1- niloevac fo noisserpxE Y otomustaM ,N amunaT ,M nhA ,C nooM ,KJ niJ ,H miK ,T nihS 21 5 31- 1 31 ,39 ,69 91 ASU icS dacA ltaN corP ylimaf eneg niloevac...

Ngày tải lên: 07/08/2014, 18:21

... tneicifed -1- niloevaC PM itnasiL ,GP knarF ,MT smailliW ,SG nassaH 711 1 -11 11 , 91 ,10 02 locnO nilC J 7059 lairT puorG ygolocnO 313 yparehT noitaidaR fo stluser :emrofitlum amotsalboilg rof noitaidar ... erew stolb ehT h rof ,)000,02 : 1( PAFG-itna dna )00 01 : 1( 1- niloevac-itna gnidulcni ,ydobitna yramirp hcae htiw detabucni neht dna h rof )lCaN Mm 0 51 dna ]4.7 Hp[ lCH-sirT Mm 01 ;SBT( enilas dereffub-sirT ... ,KJ niJ ,H miK ,T nihS 61 51- 01 ,332 ,6002 tteL recnaC amoleym elpitlum ni tegrat cituepareht wen laitnetop a sa 1- niloevaC CK nosrednA ,K radoP 51 2245- 914 5 ,372 ,89 91 mehC loiB J enarbmem amsalp...

Ngày tải lên: 07/08/2014, 18:21

... al Type and frequency of IDH1 and IDH2 mutations are related to astrocytic and oligodendroglial differentiation and age: a study of 1, 010 diffuse gliomas Acta Neuropathol 2009 ;11 8:469-74 12 Capper ... (p=0 .11 ) Molecular analyses and immunohistochemistry: IDH1 Four of the 14 0 patients (3%) showed an IDH1 mutation; all mutations were of the R132H variant The patients with a positive IDH1 mutations ... temozolomide IDH1 mutations occur in approximately 60 – 80 % of diffusely infiltrating gliomas of the WHO grades II and III and in secondary GBM but only in around % of primary GBM (11 ;18 -22) In our...

Ngày tải lên: 09/08/2014, 09:21

... MIP -1 IFN-γ TNF-α + LMP-420 (1 µM) 15 , 829 ± 75 24 ± 582 ± 54 7±3 16 , 512 ± 19 0 26 ± 17 ,16 0 ± 2 21 1,963 ± 17 51 ± 44 ± 80 ± 2,8 61 ± 98 93 ± 2, 714 ± 13 2 19 ,554 ± 63 424 ± 7,628 ± 11 2 10 ,670 ± 2 71 ... German Research Foundation (SFB 643), and MBP is an Alexander von Humboldt fellow http://www.aidsrestherapy.com/content/3 /1/ 8 14 15 16 17 18 References 10 11 12 13 Toossi Z, Johnson JL, Kanost RA, ... 13 2 19 ,554 ± 63 424 ± 7,628 ± 11 2 10 ,670 ± 2 71 18 ± 419 ± 13 3 1 17,249 ± 10 3 24 ± 17 ,575 ± 262 1, 766 ± 62 76 ± 33 ± 56 ± 4,794 ± 803 63 ± 14 3 ± 19 ,12 7 ± 520 262 ± 654 ± Percent Inhibition different...

Ngày tải lên: 10/08/2014, 05:20

... 8,879.37 11 ,12 1.66 1. 25 He et al Journal of Biomedical Science 2 011 , 18 :22 http://www.jbiomedsci.com/content /18 /1/ 22 Page of 10 Figure Regulation of miR -1 and miR -13 3a by IPost MiR -1 and miR -13 3a ... 2009, 14 :12 04 -12 11 14 Bostjancic E, Zidar N, Stajer D, Glavac D: Micrornas mir -1, mir -13 3a, mir133b and mir-208 are dysregulated in human myocardial infarction Cardiology 2 010 , 11 5 :16 3 -16 9 15 Xu ... 03 211 6 .1; rno-mir -13 3a, NR 0 318 79 .1) and AMOs (AMO -1 and AMO -13 3a) were synthesized by Jima Inc (Shanghai, China) The sequences of miRNA mimics and AMOs are showed in Table Mimic and AMO of miRNA...

Ngày tải lên: 10/08/2014, 05:21

... Gene 19 94, 14 5 :19 7-203 doi :10 .11 86 /14 76-9255-7-3 Cite this article as: Brodsky et al.: The anti-inflammatory effects of the tellurium redox modulating compound, AS1 01, are associated with regulation ... observed in the presence of AS1 01 (Fig 1) , with no detection of inhibitory effect on IKBa degradation and phosphorylation Effect of AS1 01 on LPS-induced NFkB translocalization and p50 DNA-binding ... Inflammation 2 010 , 7:3 http://www.journal-inflammation.com/content/7 /1/ 3 Page of Figure Effect of AS1 01 on NFkB translocalization LPS-stimulated RAW264.7 cells (1 × 10 6/ml) were treated with AS1 01 for...

Ngày tải lên: 11/08/2014, 08:22

... plasmid, and less than 0.5 PFU of H5N1 viruses per reaction, and specific for the detection of influenza A of subtype H5 The HA gene of clades 1, 2 .1, and 2.3 was amplified from both virus isolates and ... Singapore Table Primers and probe used in this study Name Sequencea Nucleotideb Sense 5’-TTGGTTACCATGCAAACAAYT-3’ 91- 111 Antisense 5’-TRTCTTGGGCRTGTGTAACA-3 15 2 -17 1 Probe 11 9 -14 3 5’-FAM-CAGGTTGACACAATAATGGAAAAGBHQ3-3’ ... infection and H5N1 subtyping Results Analytical sensitivity and specificity The analytical sensitivity of our LNA Taqman rRT-PCR for the detection of the HA gene of H5N1 was < 0.5 PFU of virus and 10 ...

Ngày tải lên: 12/08/2014, 04:21

... F38340 23 References 24 10 11 12 13 14 15 16 17 18 19 20 21 Carvalho CR, de Paula Pinto Schettino G, Maranhao B, Bethlem EP: Hyperoxia and lung disease Curr Opin Pulm Med 19 98, 4(5):300-304 Adamson ... effects of BQ123, FR139 317 or PD 14 5065 Br J Pharmacol 19 94, 11 1(4) :10 09 -10 16 Henry PJ, Carr MJ, Goldie RG, Jeng AY: The role of endothelin in mediating virus-induced changes in endothelinB receptor ... actions of endothelin- 1 in the guinea-pig lung: involvement of ETA and ETB receptors Br J Pharmacol 19 95, 11 5(2):227-236 Cunningham ME, Huribal M, Bala RJ, McMillen MA: Endothelin- 1 and endothelin- 4...

Ngày tải lên: 12/08/2014, 16:20

... synthase and endothelin- 1 in lungs of subjects who died with ARDS Chest 19 99, 11 6 :10 15 10 25 14 9 McMillen MA Sumpio BE: Endothelins: polyfunctional peptides J Am Coll Surg 19 95, 18 0:6 21 637 15 0 Lehoux ... responses to endothelin- 1 following inhibition of nitric oxide synthase in the conscious rat Br J Pharmacol 19 93, 11 0 :12 13 12 21 154 Schmeck J, Heller A, Groschler A, Recker A, Neuhof H, Urbascheck ... 2000, 396 :19 –22 13 0 Alanen K, Deng DX, Chakrabarti S: Augmented expression of endothelin- 1, endothelin- 3, and the endothelin- B receptor in breast carcinoma Histopathology 2000, 36 :16 1 16 7 13 1 Lahav...

Ngày tải lên: 12/08/2014, 18:20

... http://www.retrovirology.com/content/7 /1/ 32 13 7 13 8 13 9 14 0 14 1 14 2 14 3 14 4 14 5 14 6 14 7 14 8 14 9 15 0 15 1 15 2 15 3 15 4 macrophages persistently infected with human immunodeficiency virus J Virol 19 95, 69 :15 00 -15 09 Aillet ... principles of microRNA target recognition and mode of action Nat Rev Mol Cell Biol 2009, 10 :14 1 -14 8 12 1 Ambros V, Chen X: The regulation of genes and genomes by small RNAs Development 2007, 13 4 :16 35 -16 41 ... residual Le Douce et al Retrovirology 2 010 , 7:32 http://www.retrovirology.com/content/7 /1/ 32 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 HIV -1 replication in patients receiving...

Ngày tải lên: 12/08/2014, 23:23