mechanisms of calcium associated exocytosis of striatal dopamine and dopa release studied by in vivo microdialysis
Báo cáo khoa học: Modeled ligand-protein complexes elucidate the origin of substrate specificity and provide insight into catalytic mechanisms of phenylalanine hydroxylase and tyrosine hydroxylase pptx
Ngày tải lên :
31/03/2014, 07:20
... and other binding modes to be included in further analysis Results Pterin binding to the catalytic domains of PAH and TH Docking of the native cosubstrate BH4 into the crystal structure of the PAH ... Whether binding of tyrosine in this position triggers a large change in the protein structure as shown after binding of 3-(2-thienyl)-L-alanine [22] needs to be investigated Feedback inhibition by ... demonstrated by X-ray crystallography in a binary complex of truncated PAH with bound catecholamines L -DOPA, DA, noradrenaline and adrenaline [17] The position of the amine group of the catecholamine inhibitors...
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Caffeine and a selective adenosine A2A receptor antagonist induce sensitization and cross-sensitization behavior associated with increased striatal dopamine in mice pot
Ngày tải lên :
10/08/2014, 05:21
... effect of low doses of caffeine is mediated by the inhibition of adenosine A2A receptors, involving dopamine- dependent as well as dopamineindependent mechanisms, whereas higher doses of caffeine ... neuroadaptation of dopaminergic neurons in the striatum Indeed, the expression of adenosine A2A receptors in the brain is mostly limited to the striatum [20] Dopamine depletion or blockade of dopamine receptors ... RA: Dopamine, learning and motivation Nat Rev Neurosci 2004, 5:483-494 20 Wooten GF: Anatomy and function of dopamine receptors: understanding the pathophysiology of fluctuations in Parkinson's...
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Báo cáo sinh học: "Comparing the retention mechanisms of tandem duplicates and retrogenes in human and mouse genomes" pdf
Ngày tải lên :
14/08/2014, 13:21
... with the intervening spacers containing less than 10 genes, since they were confused with tandem duplicates Finally, we preserved 410 and 680 pairs resulting from We identified 1,210 tandem duplicates ... same in yeasts and mammals [22-25] To investigate the difference in gene duplicability between tandem duplicates and retrogenes in more detail, we counted the number of each type of duplicates in ... Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, PR China, 2Graduate School of the Chinese Academy of Sciences, 19 Yuquan Road, Beijing, PR China and...
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Báo cáo khoa học: " Medication errors: a prospective cohort study of hand-written and computerised physician order entry in the intensive care unit"
Ngày tải lên :
25/10/2012, 10:39
... committee Prior to introduction of CPOE, local standards of prescribing existed specifying the tenets of good practice, including the avoidance of the use of abbreviations An ME was defined to have ... rates and types of MEs and the potential outcome of intercepted and non-intercepted errors Materials and methods In April 2002, University College Hospitals London ICU introduced the QS 5.6 Clinical ... before and after implementing CPOE HWP (no of errors and % of total errors)a CPOE (no of errors and % of total errors)a Drug prescribed on incorrect drug chart section (e.g continuous IV infusion...
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Improvement of Water Quality by Removal of Suspended Solids and High-speed Biological Filtration in Closed Water Body
Ngày tải lên :
05/09/2013, 08:40
... and one windy day were met Little influence of these disturbances was found Some sampling points were selected They are the outlet of plant, the outside of experimental area, points of horizontal ... added in the improved system, and the removal of nitrogen should be considered, too References [1] Department of Water Environment, Ministry of the Environment, Japan July 2002 Outline of Administration ... heavy rain and windy day were met These disturbances had influence on temperature, SS, turbidity and the inflow of water, but they had hardly affected the factors except transparency Two roles of...
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DEVELOPMENT OF THE OZONIZER AND OZONATION TECHNOLOGY FOR WATERWORKS IN JAPAN
Ngày tải lên :
05/09/2013, 08:40
... Okada, M and Moniwa, T (1993) Kinetic Analysis On The Effects Of Dissolved Inorganic And Organic Substances In Raw Water On The Ozonation Of Geosmin And 2-MIB OZONE SCIENCE & ENGINEERING 15, 1-18 ... rate of about 70% by ozonation and entirely by BAC treatment 2) BAS is removed by about 35% by ozonation and about 80% in all processes 3) Ammonia is not removed by ozonation, but most of it ... dispersions in values of CT (C, concentrationof dissolved ozone in mg/L; parvum and T, contact time in minutes) Studies carried out by the coauthors indicate that the CT value required for 90% inactivation...
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Tài liệu ALPHABETICAL INDEX OF REPRESENTED ORGANIZATIONS AND THEIR LEGISLATIVE AGENTS BY REGISTRATION NUMBER FOR THE THIRD QUARTER 2002 docx
Ngày tải lên :
16/02/2014, 10:20
... 1192 Insurance Council of NJ 1277 Insurance Services Office Inc 755 INTEGRIS d/b/a Bull HN Information Systems 1287 Interactive Gaming Council 1193 Interhealth LLC 551 International Assn of Color ... Assn 172 NJ Business & Industry Assn 49 NJ Business & Industry Assn 382 NJ Business & Industry Assn 396 NJ Business & Industry Assn 532 NJ Business & Industry Assn 641 NJ Business & Industry Assn ... NUMBER Independent Energy Producers of NJ 868 Independent Insurance Agents of NJ 1110 Independent Pharmacy Alliance 1215 Independent School Board Members Of NJ 216 Infineum USA LP 1136 Information...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
Ngày tải lên :
19/02/2014, 02:20
... (1994) Binding and modification of proteins by methylglyoxal under physiological conditions A kinetic and mechanistic study with Nalpha-acetylarginine, Nalpha-acetylcysteine, and Nalpha-acetyllysine, ... some of these materials (e.g Ne-carboxymethyl-lysines and Ne-carboxyethyl-lysines and pentosidine) being known to accumulate with age on tissue proteins, and at an increased rate in LDL and atherosclerotic ... rate was increased by mLÆmin)1 every 10 min, and the monocyte cell fractions collected with flow rates of 15, 16, 17, 18 and, finally, 40 mLÆmin)1 were collected and combined The presence of monocytes...
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
Ngày tải lên :
19/02/2014, 07:20
... understanding of the biosynthesis of triterpene saponins is required, including the enzymes catalyzing the sequence of reactions and the genes encoding these enzymes The biosynthesis of triterpene ... out by heterologous expression in yeast Results and Discussion P450 genes induced by elicitors in the cell culture of G max Accumulation isoflavones phytoalexin glyceollins, in the seedling infected ... branching at the hydroxylation of campestanol by two independent P450 (6-hydroxylase and 22-hydroxylase) and joining at the formation of castasterone by the same enzymes was proposed in brassinosteroid...
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Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx
Ngày tải lên :
20/02/2014, 11:20
... order of magnitude lower than that of wild-type Ydc2 (compare lanes b and g, and c and h in Fig 3B) No binding to liner duplex DNA was observed using either of the truncated proteins under any of ... mutants of Ydc2–GFP were introduced in strain JAL01 Vector pREP41–EGFP in JAL01 was used as control Expression of the fusion proteins was induced by growth in the absence of thiamine and was ... mitochondrial diseases and implicated in aging (reviewed in [39]) Understanding the molecular mechanisms and pathways of mtDNA inheritance in fission yeast, alongside budding yeast and other eukaryotic...
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Societal Burden of Child Maltreatment and Public Health’s Role in Prevention docx
Ngày tải lên :
14/03/2014, 09:20
... Maltreatment Bringing focus to evidence-driven prevention by Raising visibility about health consequences and costs Ensuring the availability of valid and reliable data Filling gaps in the evidence ... trauma prevention Information for parents of newborns About the serious adverse effects of shaking an infant Guidance on how to handle a crying infant and avoid shaking Evidence Hospital-based ... needs of Florida families Provide information on ways to ensure children are safe and nurtured and live in stable environments that promote well-being Inform and instruct education and law...
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Báo cáo khoa học: Structural features and dynamics of a cold-adapted alkaline phosphatase studied by EPR spectroscopy docx
Ngày tải lên :
16/03/2014, 01:20
... [19] By combining distance and lineshape measurements, global information can be obtained and the structure of whole domains can be determined [20], with one example being lipid-embedded protein ... of a cold-adapted AP using site-directed spin-labeling in conjunction with EPR spectroscopy The effects of cysteine mutations and spin-labeling on kinetic properties and stability were also studied ... spectra of the spin-labeled cysteine variants The dotted lines indicate the spectral width of WT C67R1 and are intended to aid the eye with respect to detecting spectral broadening The splitting of...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
Ngày tải lên :
16/03/2014, 11:20
... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in ... ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the afnity of the ... In vitro analysis of NF-jB signaling pathway 46 Peet GW & Li J (1999) IkappaB kinases alpha and beta show a random sequential kinetic mechanism and are inhibited by staurosporine and quercetin...
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Báo cáo khóa học: Membrane transport of fatty acylcarnitine and free L-carnitine by rat liver microsomes pot
Ngày tải lên :
16/03/2014, 16:20
... of unlabelled carnitine, lCi [3H]carnitine and lCi [14C]inulin At each time-point, two 50 lL samples were removed quickly and transferred to tubes containing mL of ice-cold HS buffer containing ... untreated pellet after correcting for adhering medium, which was determined from the amount of associated [14C]inulin Measurement of protein content This was by a bicinchoninic acid assay kit (Sigma) ... role in the CAT reaction, which must rely on an internal, luminal pool of CoASH Transport of L-carnitine into sealed microsomal vesicles Transport of L-[3H]carnitine was studied by measurement of...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
Ngày tải lên :
17/03/2014, 10:20
... enzyme in vivo (Tables and 2) provides genetic evidence for an essential involvement of the N-terminal cysteine pair in the in vivo function A possible function of this N-terminal CXXC motif in Erv1p ... four-helix-bundle [11] Replacing C159 by a serine destroys the disulfide bridge and the introduction of a polar group probably interferes with binding of the adenine portion of the FAD that is normally ... reduced with a few grains of Na-dithionite Release of protein-bound FAD by heat and acid treatment Protein aliquots were adjusted to an A460 of 0.200 before releasing the FAD by heat and treatment with...
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Báo cáo " Bioremediation of vegetable oil and grease from polluted wastewater in dairy factory " doc
Ngày tải lên :
22/03/2014, 09:20
... concentration of soybean oil lipid activity of strains was reduced; this can be explained by the inhibition of oxygen dissolve at high concentration of soybean oil Combination of strains In facts, ... Lipid degrading activity of mixed ratios Addition of antifoam and surfactant density was decreased and it was quite inhibited at CF-18 of 1.5% w/v This can be explained by the increasing of surface ... using in wastewater treatment system In this study, the optimal concentration of CF-18 was investigated The result (Table 1) showed that increasing of CF-18 concentration caused increasing of...
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AN IMPACT EVALUATION OF EDUCATION, HEALTH, AND WATER SUPPLY INVESTMENTS BY THE BOLIVIAN SOCIAL INVESTMENT FUND pptx
Ngày tải lên :
22/03/2014, 15:20
... storing medicines and vaccines Finally, it made drinking water available and typically installed showers As explained, the sif originally intended to make investments in all health clinics in ... effect was corrected for in three rounds: first by constructing a match in the design stage, then by using propensity score matching, and finally by using a differencein-difference estimator Health: ... effectiveness of improving infrastructure quality in education (Hanushek 1995, Kremer 1995), health (Alderman and Lavy 1996, Lavy and others 1996, Mwabu and others 1993), and drinking water (Brockerhoff and...
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Báo cáo khoa học: A comparative study of type I and type II tryparedoxin peroxidases in Leishmania major pot
Ngày tải lên :
23/03/2014, 07:20
... gel and stained with Coomassie Brilliant Blue Bands of reduced and oxidized TDPX1 wild-type were excised and digested with trypsin or chymotrypsin and peptides analysed by the Mass Fingerprinting ... found in the spectrum of the reduced protein The mass of this peak can be assigned to the sum of two peptides containing two cysteine residues ()2H + 1), namely those containing the Cys35 and the ... reduced one and again the mass tted to the sum of the two peptides ()2H + 1) containing the same cysteine residues, Cys35 and Cys83 Also, in the spectra of the oxidized and reduced protein the peptides...
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Báo cáo khoa học: Modulation of Ca2+ entry and plasma membrane potential by human TRPM4b pptx
Ngày tải lên :
23/03/2014, 09:21
... Modulation of Ca2+ entry by TRPM4b R Fliegert et al phosphorylation by protein kinase C, and binding of Ca2+–calmodulin and phosphatidylinositol 4,5-bisphosphate [8,9] Nilius ... overexpressing TRPM4b (black lines) either activated by ionomycin (addition of lM final concentration of ionomycin indicated by arrow; solid lines) or untreated (dashed lines) (B) Quantitative analysis of ... kept in serum-containing medium instead of Ringer solution [16] Interestingly, the gene encoding TRPM4 has been shown to be one of the genes upregulated during the transition from prostatic intraepithelial...
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